Three preparations of dried oleander leaves were prepared. The first one was by extraction with hexane; the second by extraction with 70% ethanol while the third was suspended as a whole ground leaves in distilled water. Different concentration of each form was prepared and the range of lethal dose in mice had estimated by pilot studies. The acute toxicity study was carried out by determine the median lethal dose (LD50) after administration of each preparation using 5 subgroups (10 mice) each receive dose range from 60-64 mg/kg, 504-536 mg/kg and 1100-1300 mg/kg for the hexane extract , ethanol extract and leave suspension respectively. The lethal dose calculated by employing probit method and found to be; 62.6 mg/kg for hexane extract, 521.0 mg/kg for ethanol extract and 1164.8 for leave suspension. This result indicates that the toxic constituents of the leaves are mostly non-polar, also that the potency of toxicity is far less than that mention in some literature.

The activity of water and ethanol extracts of Ruta graveolens and Cuminum and the essentioal oils of the groups A and H were evaluated on the termites workers of Microcerotermis gabriles in the laboratory with different concentrations. Result showed that ethanol extracts of R.graveolens , C. cyminum were the most effective in mortality significant differences from other extracts in filter paper treatment which cause 71.6%, 73% respectively in concentration 10% in 24 hour and increase to 100% after 72 hour of treatment Direct method contact showed that powder of C. cyminum was the most effective in mortality with rates 66.6 % for period 10 minutes and increase to 100% after 72 hour of the same period. All tested plants extracts was repellent to termites and the essential oil of C. cyminum was the effective once.

Results of the present investigation on infection of Trypanorhychid cestode infestation of marine fishes of Khor –Abdulla North west Arabian Gulf throws June 2006 to May 2007 . revealed to presence of four different species Trypanorhychid cestodes:-. :Callitrahynchus gracilis , Dasyrhynchus pacificus from Scomberoides cammmersoniaus ; Nybelinia lamonteae from Saurida undosquamis ; Otobothrium penetratus from Synaptura orientalis ;Pterobthrium hira from Illisha elongata. which all represented as a new locality recorded parasite

This present study was designed to investigate the effects of two levels of cadmium chloride (30 and 40 part per billion (ppb)) in drinking water on body weight, relative weight of testes and epididymus (head, body, tail), diameter and thickness of seminiferous tubules. Eighteen mature male rabbits were randomly divided into three equal groups; the control group (GI) were given ordinary tap water and animals in the first treated group (GII) were received 30 part per billion (ppb) of cadmium chloride in drinking water; while animals in the second treated group(GIII) were given 40 ppb of cadmium chloride in drinking water. Weight of animals were taken at the pre-treated period and every three weeks during the treated period. At the end of experiment the animals were killed, testis and epididymus were taken in order to measuring their weight. Samples testis were taken for histological study and for measuring the diameter of the seminiferous tubules and the thickness of the cells lining their. The results showed a significant increase (p

Out of one hundreds heads of cattle slaughtered in Dohuk abattoir of Iraq – Kurdistan region , Sarcocystosis showed to be occur in percentage of 81 – 96 % for microscopic cysts( microcysts ) of Sarcocystis spp. of cattle by several diagnostic techniques ( gross examination , trichinoscopy , squeezing method and post trichinoscopy drop examination ) which are varies in their efficiency depending on organ and the parasitic stage that can be detected by the used technique . The seasonal effect showed to be not effect in the distribution of the infection and that very important in the epidemiology of the disease as occur through statistical analysis.

The present work making histological investigation of certain part of medulla oblongata on seven one humped camel ( Camelus dromedarius ) in different ages and sexes, the Trigeminal V, Abducent VI, Facial VII, and Vestibulocochlear VIII nerve nuclei in camel was noticed. These nuclei are in close resamble in other mammals in location and the cells ( neuron ) type found in these nuclei. This study help to present an information on these nuclei cytoarchitecture and thier location, and may answers of many question about this animal (Camel ) which walking across huge deserts.

Polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) are ubiquitous and can contaminate the food chain. A study monitoring PCD/Fs in imported meat was conducted at the National Veterinary Research and Quarantine Service (NVRQS, Republic of Korea) in order to maintain food safety from the bioaccumulative PCDD/Fs. Seventeen PCDD/Fs with toxic equivalency factors (TEFs) established by World Health Organization (WHO, 1998) were analyzed in imported beef, pork, and chicken by high resolution gas chromatography/mass spectrometry (HR-GC/MS). Results of the monitoring for the last 5 years are presented. The levels of PCDD/Fs were similar to other studies except two unusually high concentrations in pork and beef. Excessive levels greater than the Korean provisional maximum residue limit of PCDD/Fs were found in a sample of pork imported from Chile and a sample of beef imported from U.S, and those products were rejected and returned. These was no obvious trend or differences with respect to time or origin of meat in this study.

The trend in the prevalence of bovine tuberculosis (BTB) in Korean dairy cattle was investigated in relation to test programs used between 1961 and 2004, during which a total of 8,961,061 dairy cows were tested and 10,248 confirmed to have BTB. The annual prevalence increased in the late 1960s, then decreased during the 1970s and 1980s, and started to increase again from the late 1990s. It seemed that the prevalence varies according to the different test program used. The prevalence of BTB was higher when the tests were performed with heat-concentrated synthetic medium (HCSM) or purified protein derivative (PPD) tuberculin alone compared to that when using combined HCSM and PPD tuberculin testing.

Akabane disease is caused by an arthropod-borne viral pathogen and leads congenital abnormalities of the central nervous system in infected ruminants. One isolate, KV0505, showed cytopathic effect in Vero cells. The KV0505 isolate was obtained from plasma, which was collected from a cattle raised on Jeju Island in May 2005. Jeju Island is located near the southern part of the Korean peninsula. The isolate was confirmed as Akabane virus (AKAV) by immunofluorescence assay using AKAV specific monoclonal antibodies and reverse transcription polymerase chain reaction (RT-PCR). Suckling mice inoculated with the isolate showed signs of paralysis and died within 10 days post-inoculation. Comparisons of the KV0505 N gene sequence with 39 other known AKAV strains revealed nucleotide homologies ranging from 83.6% (MP496 strain) to 99.7% (M171 strain). When compared with the K-9 strain, which was isolated from a cow in Korea in 1994, the nucleotide sequence homology with the N gene was 99.7%. Thus, genes of the KV0505 isolate were closely related to those of the M171 strain, which were clustered into the Ic group of AKAV.

In total, 1,142 serum samples were collected from 223 goat flocks rising in five different regions of Korea. These samples were screened for the presence of border disease virus (BDV) antibodies using an enzyme linked immunosorbent assay. Of the 1,142 samples, we found 47 bovine viral diarrhea virus (BVDV) positive cases (4.1%). These positive serum samples were also examined further by using the virus neutralization test against BDV. In addition, samples were tested for both BVDV and classical swine fever virus (CSFV). All of the samples that were seropositive for BDV also demonstrated positive antibody titers against BVDV and CSFV. Due to their common antigenicity, we also determined further the prevalence and carried out virus neutralization test against three pestiviruses: 314 of the goat samples were screened using reverse transcription polymerase chain reaction with primer pairs specific to common pestivirus genome regions. Overall, 1.6% (5/314) of the samples tested was positive for pestivirus. Based on the nucleotide sequence data and the phylogenetic analysis, three isolates were characterized as BVDV type 1 and two isolates as BVDV type 2. However, none of the isolates could be classified as BDV. These results indicate that BVDV-1 and BVDV-2 are the pestivirus strains circulating among Korean goat populations.