This study aimed to evaluate and compare four double-layer closure techniques for canine gastrotomy. Twenty adult clinically healthy mongrel dogs of both sexes were involved in the present study. Dogs were randomly allocated into four groups; A, B, C, and D (n=5 for each group) based on the used technique for gastrotomy closure. In group A, the first layer of the stomach wall (mucosa and submucosa) and the second layer (tunica muscularis and tunica serosa) were closed in simple continuous suture pattern. In group B, both layers were closed using inverting suture pattern. In group C, the first layer was closed by inverting suture pattern, while the second layer was closed by simple continuous suture pattern. In the group D, the first layer was closed by simple continuous suture pattern, while the second layer was closed by stabling using the skin staplers. Physiological parameters (rectal temperature, heart rate, and respiratory rate) and the body weight were taken preoperatively, 7 and 14 days post-operatively. Dogs were euthanatized 14 days, postoperatively. The four techniques were evaluated for the procedure time, closure efficiency, postoperative complications, and histopathology.

Pathogenic bacteria causing diseases in broiler chicks are widely distributed. One hundred and fifty cases of broiler chick samples were obtained from 15 farms with complain of early mortalities. Out of them,  100 diseased and 50 freshly dead chicks aged 1 - 14 days were examined. Liver, heart blood, lung, yolk sac and thigh bones were collected . Bacteriological investigation in both diseased and freshly dead chicks revealed that the prevalence rate of E. coli was 70% with higher incidence in liver followed by yolk sac, S. aureus isolates represented as 18.5% mainly from thigh bone followed by liver, incidence of Pseudomonas aeruginosa was 20% mainly from yolk sac proceeded by liver,  Klebsiella pneumonia incidences was 13.3% with high rate from liver. Prevalence of all previous isolated bacteria was higher in freshly dead than in diseased chicks. Serological identification of 68 isolates of E. coli (64.8%) were typed with 5 different serotypes as 15 (O119:H6), 8 (O1:H7), 10 (O146:H21), 20 (O78), 8 (O29) and 7 (O144) while 37 (35.2%) were untyped.  E. coli antimicrobial resistance was performed with marked sensitivity reported with amoxicillin. PCR performed for detection of some  virulence genes mainly eaeA and iss from  E. coli isolates, and enterotoxin B (SEB) and enterotoxin D (SED) from S. aureus isolates  with positivity 100% for each genes. This study indicated presence of  some pathogenic bacteria in broiler chicks up to two weeks as  E. coli, S. aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae which cause diseases with a consequence of economic losses.

Tumors have been rarely documented in the Arabian dromedary (Camelus dromedarius). Importantly, the current study investigated multiple primary tumors in the liver of Dromedary camels, slaughtered at different abattoirs in Egypt during the period from January 2019 to February 2022. The study focused on the existence of two or more separate primary neoplasms, or a single one involving multiple sites in the same liver. The study conducted a comprehensive and accurate gross and histopathological description of the neoplastic cases. The use of special stains and diverse types of immunohistochemical-specific antibodies contributed significantly to the confirmed diagnosis of neoplastic cells. Interestingly, our results diagnosed unusual multiple primary hepatic tumors (prevalence 7/988, 0.7%), including one case each of cholangiocarcinoma-leiomyosarcoma, hemangiosarcoma-cholangiocarcinoma-leiomyoma, myelolipoma-osseous metaplasia, lymphosarcoma and three cases of leiomyomas. Detecting multiple primary hepatic tumors for the first time in the veterinary research area is a major challenge in the diagnosis and treatment strategies of tumors. Additionally, liver cirrhosis, amyloidosis, parasitic infection, and mycotic granuloma may be predisposing factors associated with increased overgrowth of primary mesenchymal hepatic tumors in camels.

Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leucosis (EBL), which is the most prevalent neoplastic disease of cattle worldwide. Few studies have been conducted on BLV detection in Egypt, and it is unknown whether BLV is prevalent in some areas. BLV seroprevalence has never been identified in Beheira province. Therefore, the main objective of this research was to determine the seroprevalence of BLV among cattle in Beheira. A total of 368 cattle plasma samples (219 dairy and 149 beef) from 6 dairy farms, 1 beef farm, and 9 slaughterhouses in eight districts covering most geographical areas of Beheira were investigated using a commercial ELISA for the detection of anti-gp51 antibodies. Data were analyzed, and the risk factors associated with BLV infection were evaluated. Out of the whole samples, 44 (11.9%) tested positive for BLV, and the seroprevalence rates in dairy and beef cattle were 31/219 (14.2%) and 13/149 (8.7%), respectively. Cattle breed had a significant risk factor on BLV seroprevalence, as in Holstein cattle, it was 21.65% (OR= 3.1, p <0.004) higher than mixed local breed (8.20%) in dairy cattle. However, Colombian cattle showed the highest seroprevalence (19.15%) among tested beef cattle breeds.  Additionally, neither age nor farming system had a potential risk on BLV seroprevalence in the tested dairy or beef cattle (p > 0.1). It is concluded that BLV infection is widespread among cattle in Beheira province's various localities, with a potential risk for cattle of foreign breeds to contract the BLV infection.

Mainstay anti-fascioliasis drugs are faced with the threat of drug resistance, hence, the need for new alternative chemotherapy for the management of the disease. The present study was conducted to determine the anthelminthic efficacy of ethanolic leaf extract of Calotropis procera and Morinda lucida against the adults of Fasciola gigantica. The ethanolic extract of air-dried pulverized leaves of C. procera and M. lucida was tested on matured flukes at different concentrations; 10, 20, 40, 50 and 100 mg/ml. The anti-Fasciola efficacy of M. lucida and C. procera extract was both time and concentration dependent (p < 0.05). The lowest death time (i.e. highest flukicidal activity) 26.67±5.77 min and 40±0.00 min was recorded at 100 mg/ml extract concentration in C. procera and M. lucida respectively. Generally, the adult flukes’ death time decreased with increase in the concentration of the plants extracts. The LC50 and LC90 of C. procera and M. lucida within 110 min exposure time were 4.61 and 10.87 mg/ml, and 24 and 46.8 mg/ml respectively. The study showed that C. procera and M. lucida have anti-Fasciola activities. The activity of C. procera however, was superior to that of M. lucida.

The aim of this study was to elucidate current perspectives of redox status in dairy cows and their effects on energy metabolites and immune-inflammatory status during the close-up period. The study was conducted on dairy cows at various stages of lactation, between November 2019 and January 2020. An observational study was conducted on 36 cows, at ~ 4 weeks (28 day ± 2 day; means ± SD) before the expected time of calving. Cows were proved healthy on both clinical and laboratory examinations. A blood sample was drawn from each cow on the 28th day before the expected time of calving to quantify selected biochemical variables. The study cows were allocated into two groups based on serum oxidative stress index (OSi) concentrations, the first group included twenty-seven cows with high values of OSi, while the second group included nine cows with low values of OSi and served as negative controls. All cows were clinically healthy and showed no signs of illness throughout the study period. Cows with high OSi had higher serum levels of advanced oxidation protein products, reactive oxygen species (ROS), malondialdehyde, superoxide dismutase, non-esterified fatty acids, β-hydroxy butyric acid, glucose, serum amyloid A, haptoglobin, and immunoglobulin G than negative controls; while had low levels of serum anti-oxidant capacity (SAC), glutathione peroxidase, vitamin E, and insulin. The results herein confirmed that cows during the close-up period were likely under oxidative stress, and the latter makes cows vulnerable to the development of negative energy balance and significant immune-metabolic alterations. The results of OSi, ROS, and SAC could be used as reference variables to assess the redox status of transition dairy cattle.

This study was conducted to evaluate the microbial aspect of some meat products by performing S. aureus count, detection of S. aureus enterotoxins as well as isolation and identification of some food poisoning bacteria. Therefore, fifty random samples of beef luncheon and kofta (25 of each) were equally collected from different localities in Gharbia governorate, then bacteriologically examined, where values of S. aureus count were 7.61×102± 0.54×102 in beef luncheon, 2.82×103± 0.39×103 (cfu/g) in kofta samples. Meanwhile, in beef luncheon enterotoxin B and enterotoxin (A&D) were detected with a percentage of 4% of each, while in kofta samples enterotoxin A, enterotoxin D, and enterotoxin (A&C) were detected with a percentage of 8%, 4%, and 4%, respectively. Moreover, Salmonellae, E. coli, Klebsiella pneumoniae, and Listeria were detected at 16%, 20%, 20%, and 20% in the examined beef luncheon. while, in kofta samples 24 %, 36%, 28% and 16%, respectively. Meanwhile, E. coli identified as O26 : H11, O44 : H18, O78, O91 : H21, O103 : H2, O111 : H4, O127 : H6, O146 : H21 and O159 with various percentages. Salmonella spp. identified as S. Enteritidis, S. Typhimurium, S. Labadi, S. Infantis, and S. Molade. On the other side, Listeria species serologically identified as L. monocytogenes, L. ivanovii, L. innocuai, and L. welshimeri. Moreover, isolated serotypes of K. pneumonia as K1 and K2 were 16%, and 4% in luncheon samples. While 16% and 12% were in kofta samples.  Furthermore, high virulent K. pneumonia (HVKP) and classic K. pneumonia (CKP) were 12% and 8% in luncheon, while were 20% and 8% in kofta samples, respectively. Achieved results in the current study proved that most of the examined meat products were contaminated with E. coli, Salmonella spp., S. aureus, Listeria spp. and K. pneumonia, this considered objectionable, as they render the product of inferior quality and unfit for consumption

Poultry bacterial pathogen is a major problem in poultry farms, with serious consequences for poultry and human. Two hundred samples of apparently health and freshly died broiler were collected from different commercial farms at Sharkia governorate, Egypt. Salmonella spp. detected in (29%) of examined specimens and serologically identified into S. Typhimurium, S. Kentucky, S. Infantis, S. Enteritidis and S. Agama with percentages 9.5%, 6.5%, 5%, 4.5% and 3.5%, respectively. Meanwhile, Pseudomonas spp. detected in (19%), the most prevalent serotype was P. aeruginosa O2, O5, O9, and O11. However, the antimicrobial-resistant strains of pathogens continuously emerge, with ineffective of medical treatments, thus, the isolates were examined for detection of multidrug resistant (MDR), doxycycline exhibited the highest antimicrobial activity against Salmonella spp. (55.17%); and ceftriaxone and doxycycline against Pseudomonas spp. (52.63%). Uniplex PCR examination for ampC, stn, tetA(B), integrase genes on MGEs were detected in all Salmonella spp. isolates, and mexR, tetA (B), integrase genes in all examined MDR P. aeruginosa isolates, meanwhile, exoU detected in 80% on MGEs. A novel antibacterial strategy was achieved to minimize economic burdens and the health associated with antimicrobial resistance which obliterate pathogens without any adverse effects on poultry and human. Therefore, the application of a trial using M. Oleifera nanoemulsion in order to control the multidrug resistant genes expression. These findings demonstrated that M. oleifera nanoemulsion was a good choice to its potential as a drug that can be used against Salmonella and P. aeruginosa in poultry industry.

For poultry farmers and quails producers’, one of the biggest challenges is dealing with natural diet contaminants like mycotoxins. Worldwide, mycotoxins are present in all feed sources, primarily in corn, and they significantly reduce the health, immune function, and performance of birds. For this purpose, the effect of citric acid (CA) supplement on contaminated diet with Aflatoxins (AFL) in the liver biochemical, histological, and ultrastructural studies of male Japanese quail (Coturnix coturnix). Influences of experimental diets were assessed in 3 replications of 6 birds each (n = 18 per treatment). Quails two weeks old were assigned into 4 equal groups. The control quails fed only basal diet, AFL group quails were given basal diet contaminated with 2.5 mg AFL/kg diet, citric group quails fed basal diet with 10 g citric acid/Kg, and AFL/citric group quails fed basal diet contaminated with 2.5 mg AFL /Kg and augmented with 10 g/Kg citric acid. After four weeks, feeding AFL to quails induced hepatotoxicity as evidenced by significant decline in body weight, serum albumin and total protein while it significantly increased serum ALT, and AST activities. AFL also induces liver oxidative stress by the elevation of lipid peroxidation and reducing GPx, ADH, SOD and catalase activities. Descriptive hepatic histological and ultrastructural alteration were also noted in the AFL group. Treatment with CA induced an increase in total protein, albumin, SOD, GPx, ADH and significantly decreased ALT and AST activities and MDA level. Moreover, it also improved the histological and ultrastructure alternations induced in the liver of AFL group. It was concluded that supplementation of CA into the AFL polluted diets lessened the adverse influences of AFL on quail’s liver.

The study investigated the effect of the administration of chitosan–nanoparticles (ChNP) on body performance, gut microbiota, some immunological parameters, expression of digestibility, and antioxidant-related genes in broilers. A total of 80 one-day-old Ross mixed-sex chicks were divided into two groups (40 birds each) in open door system: Chicks in group 1 were fed a basal diet as control, whereas chicks in group 2, were fed a basal diet, and drinking water supplemented with 50-mg ChNP/L. The experimental period lasted three weeks with a daily observation of body performance. Serum and tissue samples were collected for immunological evaluation, microbiota counts, and estimation of mRNA expression levels. ChNPs supplementation significantly increased beneficial microbiota and diminished coliforms, improved growth performance, kidney functions, total antioxidant capacity, and gene expressions of Glut, CAT1, CAT, SOD, and GPX1 in broilers. In conclusion, supplementation of ChNP led to an upregulation of body performance and antioxidant activity of broiler chicks phenotypically and genotypically.