Regional distribution and relative frequency of endocrine cells in ten portions of the gastrointestinal tract of the Korean native cattle were observed by immunocytochemical methods using specific antisera against chromogranin, serotonin, somatostatin, glucagon, bovine pancreatic polypeptide (BPP), motilin, gastric inhibitory polypetide (GIP), neurotensin, secretin, gastrin and substance P. In the abomasum, chromograin-, serotonin-, somatostatin-, motilin-, glucagon-, gastrin-, and substance P-immunoreactive cells were found. Chromogranin- and serotonin- and serotonin-immunoreactive cells were more numerous in the fundic region than pyloric region. Somatostatin- and gastrin- immunoreactive cells were numerous in the pyloric region than in the fundic region. In the large intestine, chromogranin-, serotonin- and BPP-immunoreactive cells were widely distributed and most numerous in the rectum. Somatostatin-, glucagon- and substance P-immunoreactive cells were rarely seen in the large intestine

This study was designed to elucidate the effects of iron, a well known catalyst of lipid peroxidation, on the contents of phospholipids, unsaturated fatty acids composed in phospholipid molecules and their derivatives, prostaglandins, and the composition changes of fatty acids contained in phospholipids. Iron decreased the contents of phospholipids and its components of unsaturated fatty acids. Catalytic action of iron decreased the composition rates of linoleate and linolenate composed in phospholipid molecules, while that of arachidonate was inclined to increase. The content of arachidonate was increased and that of prostaglandins was decreased without regard to increase the precursor of prostaglandins. It may be concluded that the decreases of the activities of prostaglandins and the increase of arachidonate are due to inhibition of the activities of enzyme systems responsible for prostaglandin synthesis by lipid peroxides produced by the catalyst of iron

The phenotypes of hemoglobin, albumin and transferrin of 302 Jindo dogs in Jindo area were studied by starch gel electrophoresis for hemoglobin and albumin, and by polyacrylamide gradient gel electrophoresis for transferrin. In the hemoglobin phenotypes, three phenotypes, HbAA, HbAB and HbBB, which were controlled by two allelic genes, Hb** (A) and Hb** (B), were observed and their frequencies of appearance were 1.65%, 10.60% and 87.75% respectively. The distribution of gene frequency was calculated as 0.0695 in Hb** (A) and 0.9305 in Hb** (B). In the albumin phenotypes, three phenotypes, Alb FF, Alb FS and Alb SS, which were controlled by two allelic genes, Alb** (F) and Alb** (S) were observed and their frequencies of appearance were 12.59%, 25.56% and 61.85% respectively. The distribution of gene frequency was calculated as 0.2537 in Alb** (F) and 0.7463 in Alb** (S). Analysis of transferrin phenotypes showed 6 different types which were controlled by three allelic genes, Tf** (B), Tf** (C) and Tf** (D), and their frequencies of appearance were 54.04% in TfBB, 17.54% in TfBC, 9.82% in TfBD, 8.07% in TfCC, 7.37% in TfCD and 3.16% in TfDD. The distribution of gene frequency was calculated as 0.6772 in Tf** (B), 0.2053 in Tf** (C) and 0.1175 in Tf** (D)

In order to investigate the morphological characteristics of dendritic cells in the mammary gland of the mouse (C57 BL/6), rat (W), rabbit and cat, the fine structures of the dendritic cells have been observed by the electron microscope. The dendritic cells with the well-developed processes had an irregular shape, and lacked the desmosome. The pinocytotic vesicles and tubular invaginations of the cell membrane were frequently observed, and the mitochondria with the well-developed cristae were located in the restricted region in the cytoplasm of the dendritic cells. The nuclei of dendritic cells were indented. In the mice and rats, the dendritic cells had a few langerhans cell granules (Birbeck granules). From the above results, it is confirmed that the ATPase-positive dendritic cells in the mammary gland are the langerhans cells

Blood samples were obtained from Korean native cattle and dairy cattle of Holstein species in the slaughter house and methylene blue color tests were performed for the detection of the inherited susceptibity to hemolysis. Glucose-6-phosphate dehydrogenase activities expressed as the optical density obtained by methylene blue color test were the highest as 0.54 in male Korean cattle, 0.62 in female Korean cattle and 0.72 in dairy cattle of Holstein species. Percent hemolysis, packed cell volume and plasma protein contents were measured and compared with relation to the results of methylene blue color test and no correlation was observed in each

This experiment was carried out to provide the data of blood chemistry in use of application to clinical diagnosis in racehorses. Blood was collected from 30 racehorses (female 17, male 13). Calcium, inorganic phosphorus, blood glucose, blood urea nitrogen, cholesterol, bilirubin, fibrinogen and ketone body in serum were analyzed. The values of calcium and inorganic phosphorus were maintained a certain level without much variation regardless of sex and species. Ca/P ratio was in the range of 3.13-3.35. The value of blood glucose was 60mg/100ml. Age and sex of racehorses make no difference in the value. The value of blood urea nitrogen and total cholesterol showed a tendency to increase according to the age. Total cholesterol value was somewhat higher in male than in female. The value of total bilirubin showed a tendency to increase according to the age. There was no difference between sexes in the value. The value of fibrinogen showed no apparent difference with age and sex. Ketone body was negative in all of the cases. There was no variation on the species of the racehorses

The prevalence of yeasts in mammary glands of dairy cows and teat cups of milking machines was studied in Chinju area. The rate of subclinical yeast infection in 330 quarters was 3.6 %. Of 12 isolates from the milk, 4 Candida pseudotropicalis, 3 C. tropicalis, 2 C. krusei, 2 C. albicans and 1 Rhodotorula spp were identified. The 91.7 % of the isolates belonged to the genus Candida and C. pseudotropicalis was the predominant species. From 20.5 % of 200 teat cups tested, 51 strains of yeasts were isolated. These were 13 C. pseudotropicalis, 9 C. guilliermondii, 7 C. tropicalis, 5 C. krusei, 5 C. parapsilosis, 3 C. albicans, 2 Torulopsis glabrata, 2 Geotrichum candidum and 5 unidentified yeasts. C. pseudotropicalis was most frequently encountered

Blood coagulation time, bleeding time, clot retraction ability, thrombocyte counts and hematological values under electroacupuncture anesthesia and medicament anesthesia, using 10 mongrel dogs were compared. Blood coagulation time under electroacpuncture anesthesia was shorther than that under medicament anesthesia (p0.001). Bleeding time under electroacupunture anesthesia was shorter than that under medicament anesthesia (p0.01). Clot retraction ability under electroacupuncture anesthesia was better than that under medicament anesthesia (p0.01). Thrombocyte counts under electroacupuncture anesthesia was more increased than that under medicament anesthesia (p0.05). Erythrocyte counts, hematocrit values, hemoglobin contents and leukocytes counts were decreased (p0.01) under medicament anesthesia in comparison with control group, but there was no significant difference under electroacupuncture anesthesia

To elucidate the distribution of mating type of Microsporum canis, originated from animals in Korea, a total of 272 strains of M. canis isolated from dogs and cats were mated with the tester strains of Nannizzia otae. Among 272 strains of M. canis examined, 217 strains(79.8%) were N. otae-, but none of strains showed + mating type. And 55 strains(20.0%) reacted with neither N. otae+nor N. otae-

To examine the selectivity of verapamil, used in the cardiovascular diseases, on alpha-1 and alpha-2 adrenoceptor-induced pressor responses, effects of verapamil on alpha-adrenoceptor agonist-induced pressor responses were investigated in urethane-anesthetized rabbits, spinal rabbits, rats and pithed rats. To evaluate the effects of verapamil on each pressor response induced by norepinephrine, phenylephrine and clonidine, these agonists were previously injected into an ear vein, and the same procedures were performed 1-2 min after treatment with intravenous verapamil. Intravenous verapamil produced dose-dependent depressor response in rabbits and rats. Pressor responses to intravenous norepinephrine (10 micro g/kg) and phenylphrine (30 micro g/kg) were inhibited by pretreatment with intravenous verapamil in rabbits and no difference was noted between the degree of both inhibitions of the pressor response by verapamil. Pressor responses to intravenous norepinephrine (3 micro g/kg), phenylephrine (20 micro/kg) and clonidine (300 micro g/kg) were inhibited by pretreatment with intravenous verapamil in spinal rabbits. No difference was noted between the inhibition of norepinephrine-induced pressor response and that of phenylephrine-induced pressor response by verapamil. The inhibition of clonidine-induced pressor response by verapamil was more prominent than that of norepinephrine- or phenylephrine-induced pressor response. Pressor responses to intravenous norepinephrine (3 micro g/kg) and phenylephrine (10 micro g/kg) were inhibited by pretreatment with intravenous verapamil in rats and no difference was noted between the degree of both inhibitions of the pressor response by verapamil