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Studies on the postnatal development of the Leydig cell in rabbits
2005
Tae, H.J. (Chonbuk National University, Jeonju, Republic of Korea) | Ahn, D.C. (Kangwon National University, Chuncheon, Republic of Korea) | Park, Y.J. (Chonbuk National University, Jeonju, Republic of Korea) | Kang, H.S. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, N.S. (Chonbuk National University, Jeonju, Republic of Korea) | Park, S.Y. (Chonbuk National University, Jeonju, Republic of Korea) | Yang, H.H. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, I.S. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: [email protected]
Changes in the rabbit Leydig cell from birth to adulthood were studied in New Zealand white rabbits of 1, 7, 21, 35, 49, 70, 105, 147, 196, and 252 days (n = 8 rabbits per group) of age. The objectives of this study were to understand the fate of the fetal Leydig cells, to determine the changes in serum testosterone levels, and leutenizing hormone-stimulated testosterone production per testis in vitro, and to quantify adult Leydig cells by number and average volume with age. Testes of rabbits were fixed by whole body perfusion using a fixative containing 2.5% glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite.
Show more [+] Less [-]Antioxidative effects of traditional medicinal plants on lipid peroxidation
2005
Hah, D.S. (Gyeongnam Provincial Government Institute of Health and Environment, Changwon, Republic of Korea) | Kim, C.H. (Jinju National University, Jinju, Republic of Korea) | Kim, G.S. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, E.G. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, J.S. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
To assess the antioxidative activity of 12 medicinal plants on lipid peroxidation, twelves traditional medicinal plants extracted with 95% methanol were investigated the antioxidative activity using DPPH, thiocyanate acid method, and thiobarbituric acid (TBA) methods. Out of 12 medicinal plants extracted with methanol, the extraction yields of Sedum kamtschaticum was the highest values (49.46%) among them and Geranicum sibiricum, Saururus chinensis root (R), Agrimonia pilosa leaf (L), Agrimonia pilosa root was the lowest value (9.97%). Radical scavenging effect of the selected traditional medicinal plants extracted from different extract solution were examined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical method.
Show more [+] Less [-]Rapid differentiation of Hanwoo and Holstein meat using multiplex allele specific polymerase chain reaction protocols
2005
Koh, B.R.D. (Gwangju Metropolitan Health and Environment Research Institute, Gwangju, Republic of Korea), E-mail: [email protected]
Here I describe a multiplex allele specific PCR-based approach for the rapid detection between Hanwoo and Holstein meat associated with Melanocortin 1 receptor (MC1R) gene. Specific and universal oligonucleotide primers were used in combination to detect the presence of a single nucleotide polymorphism within the bovine MC1R DNA sequence. The presence of the bovine MC1R gene is indicated by the production of a single control PCR product, whilst positive samples generate an alternative smaller specific product over the same region. The mutations in MC1R104 codon revealed depending on the presence or absence of an indicative fragment amplified from the wild-type allele of this codon.
Show more [+] Less [-]Contamination patterns of Listeria spp. in pork processing plants using random amplified polymorphic DNA
2005
Ha, S.Y. (Kangwon National University, Chunchon, Republic of Korea) | Choi, W.S. (Dongguk University, Gyeongju, Republic of Korea) | Bahk, G.J. (Korea Health Industry Development Institute, Seoul, Republic of Korea) | Hong, C.H. (Kangwon National University, Chunchon, Republic of Korea), E-mail: [email protected]
This study was carried out to understand the contamination patterns of Listeria in pork processing plants. A total of 402 samples were collected from carcass, pork during processing, surfaces of equipment and environment, and 238 isolates of Listeria species were identified. L. innocua was found in 64.7% of the isolates, L. monocytogenes in 33.2%, and L. welshimeri in 2.1%. Random amplified polymorphic DNA (RAPD) analysis performed to investigate the origin and routes of Listeria contamination, showed 21 composite types of L. monocytogenes and 26 composite types of L. innocua. It was confirmed that Listeria contamination begins with contaminated incoming carcass and ever-present contaminants in the processing environments.
Show more [+] Less [-]Localized and disseminated histiocytic sarcoma in two dogs
2005
Kwon, H.J. (Seoul National University, Seoul, Republic of Korea) | Cho, D.Y. (Louisiana State University, Baton Rouge, LA, USA) | Park, M.S. (Seoul National University, Seoul, Republic of Korea) | Oh, S.Y. (Seoul National University, Seoul, Republic of Korea) | Jee, H. (Seoul National University, Seoul, Republic of Korea) | Kim, D.Y. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected]
A male mixed breed dog with unknown age (case 1) and 5-year-old female golden retriever (case 2) were admitted to the Department of Veterinary Pathology at College of Veterinary Medicine, Seoul National University after sudden death. At necropsy, stifle region of case 1 was replaced by coalescing firm tan nodules approximately 1-3 cm in diameter. Neoplastic nodules were also presented in the lung. In case 2, the spleen was enlarged 3-5 times its normal size and numerous firm tan nodules were noted in the kidney, pericardium, diaphragm and lymph nodes. Histopathologically, the neoplastic masses of case 1 consisted of plump spindle cells having abundant cytoplasm and indistinct cell borders that formed interlacing bundle.
Show more [+] Less [-]Histopathological classification of the bovine lymphoma caused by bovine leukemia virus in Korea
2005
Yoon, S.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: [email protected] | Han, H.R. (Seoul National University, Seoul, Republic of Korea) | Lee, K.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Bae, Y.C. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Jean, Y.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kang, M.I. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, O.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Several studies have been performed on the bovine leukemia since bovine leukemia virus (BLV) had been detected in 1982 in Korea. We have conducted histopathological study on the bovine lymphoma caused by BLV because only limited results were reported on the pathological characterization of lymphoma. Lymphoma tissues were obtained from cattle necropsied and slaughtered during a designated period. Lymphoma was classified histopathologically according to the National Cancer Institute Working Formulation. Leukotic tissues consisted of fairly uniform sheets of closely packed medium to large lymphocytes without any architectural arrangement in all 30 cases.
Show more [+] Less [-]Effects of conjugated linoleic acid and selenium on body composition and serum cholesterol concentration in broiler chickens
2005
Jun, H.J. (Konkuk University, Seoul, Republic of Korea) | Kim, J.Y. (Konkuk University, Seoul, Republic of Korea) | Lim, J.J. (Konkuk University, Seoul, Republic of Korea) | Lee, S.H. (Konkuk University, Seoul, Republic of Korea) | Lee, B.H. (Konkuk University, Seoul, Republic of Korea) | Jeong, S.W. (Konkuk University, Seoul, Republic of Korea) | Park, H.M. (Konkuk University, Seoul, Republic of Korea) | Chung, B.H. (Konkuk University, Seoul, Republic of Korea), E-mail: [email protected]
The purpose of this study was to examine whether conjugated linoleic acid and selenium supplementation in broiler chicken diets would be effective, enhance indices of immune status and body weight, and modulate serum lipid concentration. Forty Hyline brown chickens, 1 weeks of age, were divided into 5 groups of 8 chickens. Chickens were fed the experimental diets supplemented with 1% CLA (conjugated linoleic acid; Group 1), 1% CLA + selenium (Group 2), 1% safflower-seed-oil as LA (Group 3), 1% safflower-seed-oil as LA + selenium (Group 4) or nothing (Control) for 4 weeks. After 4 weeks, serum, liver, spleen and abdominal fat were taken. Measurement of total immunoglobulin were executed using sandwich ELISA.
Show more [+] Less [-]Coexpression of PCNA and p21 for DNA repair in small intestinal crypt cells of mouse with ∨60Co γ-rays irradiation
2005
Hong, S.J. (Cheju National University, Jeju, Republic of Korea) | Hwang, I.S. (Cheju National University, Jeju, Republic of Korea) | Ahn, M.J. (Cheju National University, Jeju, Republic of Korea) | Shin, T.K. (Cheju National University, Jeju, Republic of Korea) | Joo, H.G. (Cheju National University, Jeju, Republic of Korea) | Park, H.J. (Cheju National University, Jeju, Republic of Korea) | Jee, Y.H. (Cheju National University, Jeju, Republic of Korea), E-mail: [email protected]
The irradiation of radioactive γ-ray induces apoptosis of radiosensitive organs for homeostasis. In this study, we investigated the repair mechanisms for homeostasis in the small intestine after cell damage by ∨60Co γ-ray irradiation. The apoptosis was most frequently observed in the crypt cells of the small intestine after four and six hours by radioactive γ-ray irradiation, and the frequency of apoptosis was proportional to the amount of irradiation. Also, the number of apototic cells was coincident with expression pattern of p53.
Show more [+] Less [-]Incidence of micronuclei in lymphocytes of pig in the high background radiation area (Cheongwon-gun and Boeun-gun)
2005
Lee, H.J. (Chonnam National University, Gwangju, Republic of Korea) | Kang, C.M. (Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea) | Kim, I.H. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, T.H. (Kyungpook National University, Daegu, Republic of Korea) | Ryu, S.Y. (Chungnam National University, Daejeon, Republic of Korea) | Jo, S.K. (Korea Atomic Energy Research Institute, Daejeon, Republic of Korea) | Kim, S.R. (Chonnam National University, Gwangju, Republic of Korea) | Lee, J.H. (Chonnam National University, Gwangju, Republic of Korea) | Kim, J.S. (Chonnam National University, Gwangju, Republic of Korea) | Kim, J.C. (Chonnam National University, Gwangju, Republic of Korea) | Kim, S.H. (Chonnam National University, Gwangju, Republic of Korea), E-mail: [email protected] | Choi, S.Y. (Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea)
Cytogenetic and hematological analysis was performed in peripheral blood obtained from pigs bred in the high background radiation areas (HBRA) (Cheongwon-gun and Boeun-gun) and a control area. The frequencies of gamma-ray induced micronuclei (MN) in the cytokinesis-blocked (CB) lymphocytes at several doses were measured in three pigs. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the swine lymphocytes exposed in vitro to radiation over the range from 0 mGy to 1,969 mGy.
Show more [+] Less [-]Morphological study on the development of the tongue in fetuses and neonates of Korean native goat (Capra hircus)
2005
Cho, C.H. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, J.H. (Konkuk University, Seoul, Republic of Korea) | Kim, C.S. (Gyeongsang National University, Jinju, Republic of Korea) | Koh, P.O. (Gyeongsang National University, Jinju, Republic of Korea) | Kang, B.I. (Gyeongsang National University, Jinju, Republic of Korea) | Won, C.K. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
This study was carried out to investigate the morphological development of the tongue in the 60-, 90- and 120-day-old fetuses and neonates of Korean native goats by light microscopy. In 60-day-old fetuses, the tongue tissues were differentiated into epithelium, lamina propria, and muscle layer. The primordia of filiform, conical, lentiform, fungiform, and vallate papillae appeared by this time, and rudiments of taste buds were observed in the epithelia of the primordia gustatory papillae. The dorsal lingual epithelia were PAS positive.
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