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Characteristic of bacterial flora from the uterus in HanWoo cattle
2014
Kim, K.J., angwon National University, Chuncheon, Republic of Kroea | Park, S.Y., angwon National University, Chuncheon, Republic of Kroea | Cho, Y.J., angwon National University, Chuncheon, Republic of Kroea | Jung, B.D., angwon National University, Chuncheon, Republic of Kroea | Park, J.J., Animal Reproduction and Biotechnology Center, Hoengseong, Republic of Korea | Hahn, T.W., angwon National University, Chuncheon, Republic of Kroea
Uterine sterilization is important for improving fertility in cattle. This study compared bacterial flora in the uterus between healthy and repeat breeder cows (RBCs). The uterine flushing of six heifers, 13 healthy HanWoo cows and eight RBCs (HanWoo) were sampled, and 15 frozen semen samples were selected. Overall, 35 bacteria were identified from in HanWoo uterine flushing and semen. The bacterial genera identified from HanWoo uterine flushing were Alloiococcus, Bacillus, Enterobacter, Enterococcus, Erysipelothrix, Gardnerella, Granulicatella, Kocuria, Pantoea, Pasteurella, Rothia, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The bacterial genera identified from HanWoo semen were Bacillus, Escherichia, Kocuria, Oligella, Pseudomonas, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The prevalence and presence of the identified bacteria between healthy cows and RBCs differed significantly. Further studies are needed to determine the role of these bacteria in the uterus of HanWoo cattle with reproductive disorder.
Show more [+] Less [-]Bovine cysticercosis and its food safety implications in Harari People's National Regional State, eastern Ethiopia Full text
2014
Terefe, Yitagele(Haramaya University) | Redwan, Feysel(Ambo University) | Zewdu, Endrias(Ambo University)
Taenia saginata cysticercosis is one of the zoonotic diseases that threaten food safety and food security, particularly in developing countries. A cross-sectional study was conducted to estimate the prevalence and cyst distribution in infected cattle, and food safety implications of Taenia saginata cysticercosis in Harari People's National Regional State, eastern Ethiopia. Post-mortem inspection of carcasses and organs of slaughtered cattle in Harar Municipal Abattoir, cyst viability tests and interviews with randomly selected meat consumers were undertaken. The post-mortem inspection showed that of the 898 local zebu cattle slaughtered for human consumption and examined for the presence of cysticerci of T. saginata, 19.7% (177/898; 95% CI = 17.2-22.5) harboured at least one cyst in the muscles or organs inspected. Of the edible anatomical sites with cysticerci, shoulder muscle, liver and heart together represented 65.4%, 66.0% and 65.4% respectively of relative prevalence, total cyst count and cyst viability. These edible sites are preferred above others by local people for preparation and consumption of raw or inadequately cooked meat dishes that are locally served as kurt, kitffo and dullet. The interviews revealed that among the 300 study participants, 182 (60.7%) had been infected by taeniosis at least once during the previous year and of these 99.0% had eaten raw or undercooked beef, the majority (88.3%) obtained from butchers assumed to provide officially inspected meat that was fit for consumption. This indicated that existing meat inspection processes were inadequate to prevent carcasses infected with T. saginata cysticerci from reaching consumers. The high prevalence of viable cysts in the edible parts of beef together with the widespread consumption of raw or undercooked beef indicated the importance of T. saginata cysticercosis as a food safety problem in eastern Ethiopia. The promotion of policies to upgrade existing meat inspection procedures and public education to ensure effective prevention of T. saginata taeniosis in humans were recommended.
Show more [+] Less [-]The changing landscape for health research in Africa: The focus of the Southern African Centre for Infectious Diseases and Surveillance Full text
2014
Rweyemamu, Mark M(Sokoine University of Agriculture) | Karimuribo, Esron D(Sokoine University of Agriculture) | Mboera, Leonard E.G(National Institute for Medical Research)
Screening for foot-and-mouth disease virus in livestock-wildlife interface areas of Tanzania Full text
2014
Peter, Emma(Sokoine University of Agriculture) | Kasanga, Christopher J(Sokoine University of Agriculture) | Sallu, Raphael(Tanzania Veterinary Laboratory Agency) | Mathias, Mkama(Tanzania Veterinary Laboratory Agency) | Yongolo, Mmeta(Tanzania Veterinary Laboratory Agency) | Mulumba, Misheck(Southern African Development Community Secretariat) | Ranga, Ezekia(Ministry of Livestock Development and Fisheries) | Wambura, Philemon N(Sokoine University of Agriculture) | Rweyemamu, Mark M(Sokoine University of Agriculture)
Full genome sequencing to study the evolutionary characteristics of foot-and-mouth disease virus in southern Africa Full text
2014
Kasanga, Christopher J(Sokoine University of Agriculture) | Valdazo-González, Begoña(The Pirbright Institute) | Dwarka, Rahana(University of Pretoria) | Wadsworth, Jemma(The Pirbright Institute) | Knowles, Nick J(The Pirbright Institute) | Wambura, Philemon N(Sokoine University of Agriculture) | Rweyemamu, Mark M(Sokoine University of Agriculture) | Mulumba, Misheck(Southern African Development Community Secretariat) | Deve, Jimis(Ministry of Agriculture and Livestock) | King, Donald P(The Pirbright Institute)
The changing landscape of the molecular epidemiology of foot-and-mouth disease virus in southern Africa north of Limpopo and east Africa Full text
2014
Kasanga, Christopher J(Sokoine University of Agriculture)
The risk factors for human cysticercosis in Mbulu District, Tanzania Full text
2014
Mwang'onde, Beda J(University of Dar es Salaam) | Nkwengulila, Gamba(University of Dar es Salaam) | Chacha, Mwita(University of Dar es Salaam)
The objective of this study was to explore the reasons for the persistence of human cysticercosis (HCC) transmission in Mbulu District, northern Tanzania. The study was carried out in 25 villages, whereby five major risks were identified. The risks were indiscriminate defaecation and improper use of toilets; a free-range system of keeping pigs; indiscriminate or unregulated slaughtering and inadequate meat hygiene and inspection; consumption of undercooked and porcine cysticerci infected pork; and social structure and roles. All of the identified risks were backed up by the immanent lifestyles of the community involved. These findings are important for the development of intervention strategies in the study area.
Show more [+] Less [-]The quest for One Health: Human Resource training aspects Full text
2014
Kiwara, Angwara(Muhimbili University of Health and Allied Sciences) | Semakafu, Ave-Maria(Muhimbili University of Health and Allied Sciences) | Frumence, Gasto(Muhimbili University of Health and Allied Sciences)
Appropriately trained Human Resources for Health (HRH) are key inputs into One Health. '... more than 50% of all infectious diseases of humans originate from animals and that, of the emerging diseases about 75% could be traced back to animal origin' (Rweyemamu et al. 2006). A comprehensive understanding of the social determinants of health, through an appropriate training model for HRH, is a key input. This study aimed to explore if human and veterinary medical schools were using such a model or providing time for this model in their curricula. Specific objectives were to: determine the time that human and veterinary medical schools' curricula provide for subjects or courses related to the social determinants of health; analyse the curricula contents to establish how they relate to the social determinants of health; and explore how a bio-medical model may influence the graduates' understanding and practice of One Health. A review of human and veterinary graduate-level medical schools' curricula in East Africa was performed in April 2013 and May 2013. The findings were: in the curricula, SDH contents for knowledge enhancement about One Health are minimal and that teaching is Germ Theory model-driven and partisan. Out of the total training time for physicians and veterinarians, less than 10% was provided for the social determinants of health-related courses. In conclusion, the curricula and training times provided are inadequate for graduates to fully understand the social determinants of health and their role in One Health. Furthermore, the Germ Theory model that has been adopted addresses secondary causes and is inappropriate. There is a need for more in-depth model. This article suggests that a vicious cycle of ill-health model must be taught.
Show more [+] Less [-]Evaluation of commercial polyclonal- and monoclonal-antibody-based immunohistochemical tests for 2 genotypes of Porcine circovirus type 2 and comparison with in-situ hybridization assays Full text
2014
Seo, Hwi Won | Han, Kiwon | Oh, Yeonsu | Kang, Ikjae | Park, Changhoon | Joo, Hye Eun | Kim, Sung-Hoon | Lee, Bog-Hieu | Chae, Chanhee
The objective of the present study was to evaluate polyclonal- and monoclonal-antibody-based immunohistochemical (IHC) tests for the detection of 2 genotypes of Porcine circovirus type 2 (PCV2), a and b, in formalin-fixed, paraffin-embedded lymph-node tissue from pigs with experimental or natural postweaning multisystemic wasting syndrome and to compare the IHC results with those of in-situ hybridization (ISH) assays. The ISH assays proved more sensitive than the IHC tests for the detection of PCV2a and PCV2b. According to these findings, polyclonal-antibody-based IHC testing is the most practical routine diagnostic method for the detection of PCV2 regardless of genotype because IHC testing is less technically complex than ISH testing. However, ISH assays are useful to differentiate between PCV2a and PCV2b in surveillance programs for the monitoring of PCV2 in swine herds.
Show more [+] Less [-]ANATOMICAL DESCRIPTION OF ORBITAL REGION IN ONE HUMPED CAMEL (camelusdramadarius ) Full text
2014
Masarat .S.Almayahi
This work is important for the demonstration the orbital region as far as the fine anatomical description site of view which have a great important for veterinarian surgeon who work s in the field of eye surgery. In this study we use six skulls of adult camels ofeachsex and by mean of fine topographical and gross anatomical description of the orbital bone and foraminae, they revealed that, the supraorbital foramina was absent at the skull of one humped camel, with the presence of super facial fissure which engaged with semi foramina for transmission of supra orbital nerve.
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