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Bile composition of healthy cats and cats with suspected hepatobiliary disease using point-of-care analyzers: A prospective preliminary study Full text
2020
Huve, Romain | O'Toole, Elizabeth | Gara-Boivin, Carolyn | Fontaine, Pascal | Belanger, Marie-Claude
Although hepatobiliary disease is common in cats, little is known about the bile composition in either these diseased states or in healthy cats. The objectives of this study were to evaluate several analytes from the bile of healthy cats and to investigate the usefulness of measuring these variables to predict bacterial cholangitis. Cats were prospectively enrolled and divided into 3 groups: 21 healthy cats (group 1) and 14 cats with suspected hepatobiliary disease: 9 without bacterial biliary infection (group 2) and 5 with bacterial biliary infection (group 3). Percutaneous ultrasound-guided cholecystocentesis was conducted on each cat. Bile cytology and culture were carried out and bile was analyzed for pH, lactate, and glucose levels using several point-of-care (POC) devices. Reference values for several bile analytes in healthy cats were calculated and are presented in this study. Neither the pH (P = 0.88) nor the lactate concentration (P = 0.85) was significantly different among the 3 groups. Sodium concentration was significantly higher in group 3 than in group 2 (P < 0.05). Bile pH, lactate, and glucose levels were unable to predict the presence of a bacterial infection in the bile.
Show more [+] Less [-]L-lactate in cerebrospinal fluid can be used as a biomarker of encephalitis in cattle Full text
2020
Curti, Juliana M. | Queiroz, Gustavo R. | Pereira, Priscilla F. V. | Anjos, Mayara C. | Flaiban, Karina K. M. C. | Lisboa, Julio A. N.
Cerebrospinal fluid (CSF) changes are significant for antemortem diagnoses of some neurological diseases. The aim of this study was to evaluate if the concentration of L-lactate in CSF could be used to differentiate healthy from encephalitic cattle. Cerebrospinal fluid samples from healthy cattle (n = 10) and from those naturally affected by rabies (n = 15), bovine herpesvirus type 5 meningoencephalitis (n = 16), histophilosis (n = 6), or bacterial encephalitis (n = 4), including 1 case of listeriosis, were collected and analyzed. Physical, biochemical (i.e., protein and glucose), and cellular analyses were performed in fresh samples. L-lactate, electrolytes (sodium, potassium, and chloride), calcium, and magnesium concentrations were measured in CSF samples that were kept frozen. L-lactate concentrations were also measured in plasma. Analysis of variance was used for comparison between groups and receiver operating characteristic analysis was performed considering L-lactate in CSF of healthy versus encephalitic cattle. The CSF L-lactate concentration was significantly higher in cattle with bacterial encephalitis than in healthy cattle; however, it did not differ between viral and bacterial encephalitis. The calcium concentrations were lower in cattle with encephalitis. L-lactate concentration in CSF > 3.6 mmol/L can be accepted as a cut-off value to indicate encephalitis. Thus, L-lactate in CSF is important for the diagnosis of encephalitis in cattle. Despite the small number of cases of bacterial encephalitis, it is suggested that L-lactate was not important for the differentiation between viral and bacterial encephalitis. Additional studies with a greater number of observations are necessary to clarify this, specifically in cases of listeriosis.
Show more [+] Less [-]Prevalence and mutation analysis of the spike protein in feline enteric coronavirus and feline infectious peritonitis detected in household and shelter cats in western Canada Full text
2020
McKay, L. A. | Meachem, M. | Snead, E. | Brannen, T. | Mutlow, N. | Ruelle, L. | Davies, J. L. | Van der Meer, F.
Feline infectious peritonitis (FIP) is a fatal disease for which no simple antemortem diagnostic assay is available. A new polymerase chain reaction (PCR) test has recently been developed that targets the spike protein region of the FIP virus (FIPV) and can identify specific mutations (M1030L or S1032A), the presence of which indicates a shift from feline enteric coronavirus (FeCV) to FIPV. This test will only be useful in the geographical region of interest, however, if the FIP viruses contain these mutations. The primary objective of this study was to determine the presence of the M1030L or S1032A mutations in FeCV derived from stool samples from a selected group of healthy cats from households and shelters and determine how many of these cats excrete FeCV. The secondary objective was to evaluate how often these specific FIPV mutations were present in tissue samples derived from cats diagnosed with FIP at postmortem examination. Feline enteric coronavirus (FeCV) was detected in 46% of fecal samples (86/185), all were FeCV type 1, with no difference between household or shelter cats. Only 45% of the FIPV analyzed contained the previously reported M1030L or S1032A mutations. It should be noted that, as the pathological tissue samples were opportunistically obtained and not specifically obtained for PCR testing, caution is warranted in interpreting these data.
Show more [+] Less [-]Comparative study of the virulence of 3 major Korean porcine circovirus type 2 genotypes (a, b, and d) Full text
2020
Cho, Hyejean | Kang, Ikaje | Oh, Taehwan | Yang, Siyeon | Park, Kee Hwan | Min, Kyung-Duk | Ham, Hee Jin | Chae, Chanhee
The objective of this study was to compare the virulence of 3 major Korean porcine circovirus type 2 (PCV2) genotypes in terms of clinical signs, PCV2 viremia and antibody titers, lymphoid lesions, and PCV2-antigen within lymphoid lesions in experimentally infected pigs. Pigs were infected at 7 weeks with PCV2a, PCV2b, and PCV2d strains and necropsied at 28 days post-infection. No statistical differences were observed in clinical signs, PCV2 viremia and antibody titers, lymphoid lesions scores, and numbers of PCV2 antigens among the 3 major Korean PCV2 genotypes. The results of this study indicate that the 3 major Korean PCV2 genotypes, PCV2a, PCV2b, and PCV2d, have similar virulence.
Show more [+] Less [-]A descriptive study of the histopathologic and biochemical liver test abnormalities in dogs with liver disease in Thailand Full text
2020
Assawarachan, Sathidpak N. | Maneesaay, Phudit | Thengchaisri, Naris
The present study describes the serum biochemical alterations and histopathological abnormalities in the liver tissue of dogs with liver disease. A survey of hepatic lesions was conducted using ultrasound-guided percutaneous needle biopsies. The hematologic and biochemical changes in dogs with liver lesions were recorded. Chronic hepatitis was the most common liver histopathologic finding (37.9%). Other common findings included liver fibrosis (19.5%), vacuolar hepatopathy (10.3%), cholangiohepatitis (9.2%), hepatocellular carcinoma (4.6%), cholangitis (3.4%), cholangiocarcinoma (2.3%), and congestive hepatopathy (2.3%). Greater than 2-fold elevation of serum alanine aminotransferase (ALT) was a useful indicator, with a sensitivity of 40% to 65% for diagnosing all liver pathologies. Greater than 2-fold elevation of serum alkaline phosphatase (ALP) without significant elevation of ALT was useful for diagnosing liver diseases affected by inflammatory or regressive changes (sensitivity of 40% to 50%). Elevation of ALT, ALP, or a combination of ALT and ALP had a high sensitivity of up to 90% for identifying dogs with liver pathology. Hepatic injury and cholestasis enzymes should be interpreted together with patient history, clinical signs, and liver ultrasonographic appearance before performing a liver biopsy.
Show more [+] Less [-]Molecular diagnosis using RNAscope in-situ hybridization in canine malignancies Full text
2020
Shiomitsu, Keijiro | Bechtel, Sandra M. | Thompson, Patrick M. | Frasca, Salvatore Jr
Immunohistochemistry has been used extensively to evaluate protein expression in clinical and research settings. However, immunohistochemistry is not always successful in veterinary medicine due to the lack of reliable antibody options, poor tissue preservation, labor-intensive staining, and antigen-retrieval optimization processes. RNAscope in-situ hybridization (ISH) is a powerful technology that uses a specific sequence probe to identify targeted mRNA. In this study, we demonstrate RNAscope ISH in 4 common canine malignancies, which are traditionally diagnosed by histopathology and immunohistochemistry. Probes were designed for commonly targeted mRNA markers of neoplastic tumors; these included c-kit in mast cell tumor, microphthalmia-associated transcription factor in malignant melanoma, ionized calcium-binding adapter molecule(-1) in histiocytic sarcoma, and alkaline phosphatase in osteosarcoma. A strong staining signal was obtained by these 4 targets in each canine malignancy. These results support the use of RNAscope ISH for definitive diagnosis in canine malignancies.
Show more [+] Less [-]Analgesic efficacy of an intravenous constant rate infusion of a morphine-lidocaine-ketamine combination in Holstein calves undergoing umbilical herniorrhaphy Full text
2020
Hartnack, Amanda K. | Neihaus, Andrew J. | Lakritz, Jeffrey | Coetzee, Johann F. | Kleinhenz, Michael D.
OBJECTIVE: To assess the analgesic efficacy of an IV constant rate infusion (CRI) of a morphine-lidocaine-ketamine (MLK) combination in calves undergoing umbilical herniorrhaphy. ANIMALS: 20 weaned Holstein calves with umbilical hernias. PROCEDURES: Calves were randomly assigned to receive a CRI of an MLK solution (0.11 mL/kg/h; morphine, 4.8 μg/kg/h; lidocaine, 2.1 mg/kg/h; and ketamine, 0.42 mg/kg/h) for 24 hours (MLK group) or 2 doses of flunixin meglumine (1.1 mg/kg, IV, q 24 h) and a CRI of saline (0.9% NaCl) solution (0.11 mL/kg/h) for 24 hours (control group). The assigned CRI was begun after anesthesia induction. A pain-scoring system and incisional algometry were used to assess pain, and blood samples were obtained to measure serum cortisol concentration at predetermined times for 120 hours after CRI initiation. RESULTS: Mean pain scores did not differ significantly between the MLK and control groups at any time. Mean algometry score for the MLK group was significantly greater (calves were less responsive to pressure) than that for the control group at 4 hours after CRI initiation. Mean cortisol concentration decreased over time for both groups and was significantly greater for the MLK group than the control group at 1, 4, and 18 hours after CRI initiation. CONCLUSIONS AND CLINICAL RELEVANCE: A CRI of MLK provided adequate postoperative analgesia to calves that underwent umbilical herniorrhaphy. However, the technical support required for CRI administration limits its use to hospital settings. Kinetic analyses of MLK infusions in cattle are necessary to establish optimal dosing protocols and withdrawal intervals.
Show more [+] Less [-]Ventilated postmortem computed tomography to evaluate the lungs of dogs with and without focal lung lesions Full text
2020
Lau, Michelle Pai Yan | Yong Foo Timothy Siang | Podadera, Juan Manuel | Makara, Mariano
OBJECTIVE To identify the optimal ventilation pressure for ventilated postmortem CT assessment of the lungs in cadaveric dogs and compare the optimal ventilation pressures between dogs with and without focal lung lesions. SAMPLE 12 cadaveric dogs. PROCEDURES CT was performed with dogs positioned in sternal recumbency within 30 to 180 minutes after death. After orotracheal intubation, lungs were aerated to ventilation pressures of 0, 10, 15, 20, 25, 30, and 35 cm H2O. Lung attenuation measurements were made at 5 predetermined anatomical locations with use of a multi-image analysis graphic user interface tool. Lungs were considered hyperaerated (−1000 to −901 HU), normo-aerated (−900 to −501 HU), poorly aerated (−500 to −101 HU), and nonaerated (−100 to 100 HU) on the basis of lung attenuation values. Optimal ventilation pressure was defined as the pressure at which the percentage of normo-aerated lung was greatest. For analysis, dogs were assigned to one group when focal lung lesions were evident and to another group when lesions were not evident. RESULTS Median optimal ventilation pressure was significantly higher for those dogs with lung lesions (35 cm H2O), compared with those without (25 cm H2O). CONCLUSIONS AND CLINICAL RELEVANCE A ventilation pressure of 35 cm H2O may be considered for ventilated postmortem CT to determine the presence of focal lung lesions; however, further investigation is required.
Show more [+] Less [-]Development and assessment of a novel ex vivo corneal culture technique involving an agarose-based dome scaffold for use as a model of in vivo corneal wound healing in dogs and rabbits Full text
2020
Berkowski, William M. Jr | Gibson, Daniel J. | Craft, Serena L. | Whitley, Robert D. | Schultz, Gregory S. | Plummer, Caryn E.
OBJECTIVE To develop and assess a novel ex vivo corneal culture technique involving an agarose-based dome scaffold (ABDS) for use as a model of in vivo corneal wound healing in dogs and rabbits. SAMPLE Corneas from clinically normal dogs (paired corneas from 8 dogs and 8 single corneas) and rabbits (21 single corneas). PROCEDURES 8 single dog corneas (DCs), 1 DC from each pair, and 10 rabbit corneas (RCs) were wounded with an excimer laser; 1 DC from each pair and 11 RCs remained unwounded. Corneas were cultured for 21 days on ABDSs (8 pairs of DCs and all RCs) or on flat-topped scaffolds (8 single DCs). The surface area of corneal fluorescein retention was measured every 6 (DCs) or 12 (RCs) hours until full corneal epithelialization was detected. Changes in corneal clarity were evaluated at 0, 7, 14, and 21 days. RESULTS Median time to full epithelialization for wounded dog and rabbit corneas was 48 and 60 hours, respectively; among wounded DCs, time to full epithelization did not differ by scaffold type. After 21 days of culture on ABDSs, all DCs and RCs that epithelialized developed a circular, diffuse, cloud-like pattern of optical haze, whereas DCs cultured on flat-topped scaffolds developed a focal, crater-like region of optical haze. All corneas on the ABDSs maintained convex curvature throughout the study. CONCLUSIONS AND CLINICAL RELEVANCE Wounded ex vivo DCs and RCs cultured on ABDSs reliably epithelialized, formed optical haze (consistent with in vivo wound healing), and maintained convex curvature. This culture technique may be adaptable to other species.
Show more [+] Less [-]Anatomic Characteristics and Locations of Nutrient Foramen in Humerus of Domestic Animals Full text
2020
Yılmaz, Osman
This study aimed to determine the numbers, directions, localizations, diameters, morphometric values of the nutrient foramina (NF) in humerus of domestic mammals and to reveal the differences between the right and left humerus in animal species. In the study, a total of 223 humerus, large ruminants (56), small ruminants (60), equidae (29), sus (24), carnivora-dog (42), and carnivora-cat (12), were examined in the Department of Anatomy, Faculty of Veterinary Medicine, Van Yuzuncu Yil University. The numbers, shapes, directions, localization sites and localized surfaces of the NF’s were observed with the naked eye, and recorded. The locations of the NF’s were confirmed by calculating the Foraminal Index (FI). The diameters of the NF’s were measured using 1.2 mm (18 Gauge: G), 0.9 mm (20 G), 0.7 mm (22 G), 0.55 mm (24 G), and 0.1 mm (34 G) needles. In animal species, morphometric measurements were taken such as total length of the humerus (TLH), distance between the NF with the proximal end of the humerus (NFP), distance between the NF with the distal end of the humerus (NFD), FI and performed statistical analysis of the measured values.There was found a single NF in 99% of the examined humerus in the study. In general, it was seen that the NF’s were directed downwards, and located in the middle 1/3 with lower 1/3 segments. NF’s were determined to be localized to the facies caudalis in 100% of sus, in 93% of large ruminants and carnivoradogs, and in 85% of small ruminants; however, in equidae and carnivora-cats were all localized to the margo medialis. According to the statistics, no statistically significant difference (p>0.05) was observed between the right and left humerus NF measurement values in terms of morphometric properties. But only, the diameter of the NF in the small ruminants was statistically significant (p<0.05).It was found that the morphological and morphometric differences of NF’s in right and left humerus of domestic mammals. Moreover, in these animals, it is thought that the study may help veterinary clinicians and surgeons in evaluating of the pathological conditions related to humeral NF and planning of the operative applications to be performed in this region.
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