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Vigilância Epidemiológica da presença do vírus da raiva a partir do exame de imunofluorescência direta aplicada ao tecido cerebral de uma amostra da população canina do município de Mogi-Guaçu, SP, Brasil: ensaio probabilístico a partir de série histórica de 1989 a 1999 | Epidemiologic surveillance of the rabies virus using the brain immunoflorescence test on a sample of a canine population: probality and analysis with actual cases from Mogi-Guaçu, SP, Brazil, During the 1989-1999 period Full text
2005
Haroldo de Barros Ferreira Pinto | Omar Miguel | Alair Assis
Foi analisada a validade do exame laboratorial sistemático do sistema nervoso de uma amostra da população canina de uma dada área, como estratégia destinada a vigilância epidemiológica da circulação do vírus da raiva. Foi empregado o banco de dados do município de Mogi-Guacú, SP, Brasil, referente a série histórica compreendida entre janeiro de 1989 a dezembro de 1999. Neste período foram examinados 1167 animais dos quais 130 (11,2%) foram positivos ao teste de imunofluorescência aplicada a raiva. O tamanho da amostra para a detecção de pelo menos um animal positivo foi calculado pela fórmula n = {1-(1-±)1/d} (N - d/2) + 1. No período de 1989 a 1994 o tamanho da amostra foi calculado a partir do número real de casos registrados. Nos anos de 1995 a 1999 como não houve novos casos de raiva canina, a análise considerou hipoteticamente a presença de um caso confirmado. Também foi efetuada a simulação do número de casos de raiva que deveriam ocorrer para que a amostra efetivamente utilizada pelo Serviço de controle da raiva fosse capaz de revelar a presença de pelo menos um animal positivo. Os resultados obtidos demonstraram que no período de 1989 a 1994 em que a freqüência anual de casos de raiva canina variou de 5 a 75 o tamanho das amostras ideal seria de 12.400 a 12.922; já no período de 1995 a 1999, em que não foram diagnosticados casos de raiva canina, se ocorresse pelo menos um registro, o tamanho da amostra seria de 13.257 a 14.698. Do exposto depreende-se que em termos probabilísticos, a estratégia proposta não é indicada para a vigilância epidemiológica da presença do vírus da raiva, quando em situação de controle, pois o número de animais a serem examinados é inviável para situações concretas. | The validty of a systematic laboratory exam that consists in examining the nervous system of a canine population sample in a given area was anlyzed, as a proper strategy for epidemiological survilance of the rabies virus presence. The analysis was based on the databank of the County of Mogi-Guaçu, SP, Brazil, referring to the historical period between January 1989 and december 1999. During this period 1,167 animals were examined and an immunofluorescence test applied to rabies showed that 130 animals (11.2%) were positive. The sample size for detecting at least one positive animal was calculated by using the formula n = {1-(1-±)1/d } (N - d/2} + 1. Between 1989 and 1994 the size of sample was calculated based on the real number of recorded cases. Between 1995 and 1999, as there were no new cases of canine rabies, the analysis considered a hypothetical presence of a confirmed case. It was also carried out a simulation of the number of rabies cases that should occur so that the sample effectively used by the Rabies Control Service would be able to reveal the presence of at least one positive animal. Results showed that in the period from 1989 to 1994, in when the annual frequecy of canine rabies cases varied from 5 to 75 cases, the ideal size of sample should be from 12,400 to 12,922. In the period from 1995 to 1999, when no canine rabies cases were recorded, the sample size would be from 13,257 to 14,698 if at least one case occurred. Thus, one can understand that in therms of probability, the proposed strategy is not recommended for the epidemiological survilance of rabies virus presence, since the number of animal to be examined is not feasible in real situation.
Show more [+] Less [-]Uso de acondicionamento ambiental para matrizes suínas em gestação | Environmental modification for gestating sows Full text
2005
Patrícia de Sousa | Irenilza de Alencar Nääs
O efeito das altas temperaturas sobre os animais tem sido extensivamente estudado a fim de se obter a situação ideal de alojamento, com melhor desempenho, tanto produtivo como reprodutivo. O objetivo deste trabalho foi avaliar o efeito do resfriamento adiabático associado à ventilação controlada (SRAVC), utilizado no alojamento de matrizes suínas gestantes, no seu desempenho reprodutivo. Foram comparados dois tipos de sistemas SRVC e ventilação natural (Natural). Um total de 144 matrizes Dalland® foram alojadas em duas diferentes estações, verão e inverno, resultando em 4 tratamentos (33 matrizes cada): sistema controlado com ventilação forçada e nebulização durante o verão (SRAVC/Verão); sistema de ventilação natural durante o verão (Natural/Verão); sistema controlado com ventilação forçada e nebulização durante o inverno (SRAVC/Inverno) e sistema de ventilação natural durante o inverno (Natural/Inverno). O sistema era regulado a partir de um termostato onde os ventiladores eram acionados a uma temperatura ambiente de 25ºC e a nebulização a 27ºC, permanecendo ligada por cinco minutos e desligada durante 20 minutos. Foram avaliados os seguintes parâmetros produtivos: número de leitões nascidos vivos (NV), número de mumificados (MF), número de natimortos (NM), peso médio ao nascer (PN), número de leitões desmamados (NLD), peso médio ao desmame (PD) e ganho de peso dos leitões (GP). O sistema SRAVC proporcionou a redução do número de leitões mumificados no plantel, enquanto a estação do ano contribuiu para melhorar no peso ao desmame dos leitões. | The effect of high environmental temperature on animals has been extensively studied in order to search the ideal housing and better performance both productive and reproductive. The objective of this research was to evaluate the effect of the evaporative cooling system associated to controlled ventilation (SRAVC) used in gestating sow's housing on their reproductive parameters response. Two systems were evaluated: SRAVC and natural ventilation (Natural). A total of 144 Dalland® sows were used during two seasons, summer and winter, resulting in 4 treatments (33 sows each) such as: the use of evaporative cooling associated to controlled ventilation during summer (SRAVC/summer), natural ventilation during summer (Natural/summer), evaporative cooling associated to controlled ventilation during winter (SRAVCwinter) and natural ventilation during winter (Natural/winter). The SRAVC system control was adjusted by thermostat, and the ventilation started when the ambient temperature reached 25ºC, and fogging at 27ºC, keeping it on for five minutes and then turning it off for the next twenty minutes. The following reproductive parameters were evaluated: number of piglets born alive (NV); mortality of embryo (MF), mortality at birth (NM), weight at birth (PN), number weaned piglet (NLD), weaned piglet's weight (PD), and piglet's weight gain (GP). The forced ventilation system decreased the embryo mortality, but the only the season influenced the final weight of weaned piglets.
Show more [+] Less [-]Caprine arthritis encephalitis: productive and reproductive aspects evaluation in infected and no infected goats | Artrite encefalite caprina: avaliação dos aspectos produtivos e reprodutivos de animais infectados e não infectados Full text
2005
Elizabeth Bohland | José Luiz D'Angelino
The present study was carried out to compare the productive and reproductive aspects of Caprine Arthritis Encephalitis virus (CAEv) antibody-positive and antibody-negative dairy goats. The agar gel immunodiffusion test (AGID) was performed, using the following antigens from the CAEv: gp135, a glicoprotein and p28, an internal structural protein. A goat herd coming from the contry of São Paulo state was evaluated for 24 months. Three serum tests were performed with intervals of 12 months. The frequency of occurrence of CAE, obtained for the three different moments of observation was 60,1%, 74,1%, 70,5%, respectively, and the total rate was 68.3% (566/829). The percentage of reagents to the CAEv rose significantly as the age grew. The average lactation length (in days) was lower for positive goats, significant values were found for goats of 24 to 36 months and for 36 to 48 months ones. The CAEv antibody-positive goats presented a mean daily milk yield (kg./day) lower than CAEv antibody-positive goats, with significant values for the groups: 12 to 24 months and 48 months or more. The total production for lactation was 21.5% lower for positive goats. The CAE did not influenced the reproductive aspects in this study on first kidding and on kidding interval. However, the average of kidding age was bigger between the reagents, when compared with not reagents, being statistically significant. Also the weight of kids, born from positive goats with age of 48 months or higher, was lower than that of the ones born from negative mothers. | O objetivo foi avaliar os aspectos produtivos e reprodutivos, de caprinos leiteiros, reagentes e não reagentes à prova de imunodifusão em ágar gel (IDAG), utilizando os antígenos gp135 e p28 do vírus da Artrite Encefalite Caprina (CAE). Os animais pertenciam a uma propriedade localizada no interior de São Paulo e foram acompanhados por um período de 24 meses, tendo sido realizados 3 testes sorológicos, com intervalos de 12 meses. A freqüência de ocorrência da CAE, nos três momentos foi respectivamente, 60,1%, 74,1%, e 70,5% (média de 68,3% - 566/829). O percentual de animais reagentes aumentou significativamente com a idade. A duração média da lactação (dias) foi menor para reagentes e significativa nas faixas etárias 24 a 36 meses e 36 a 48 meses. A produção leiteira (kg./dia) foi menor para reagentes e significativa nas faixas etárias: 12 a 24 meses maior ou igual a 48 meses. A produção total por lactação foi 21,5% menor para as cabras soropositivas. A CAE não influenciou os aspectos reprodutivos neste estudo no que se refere à idade ao primeiro parto, ao intervalo interpartos. No entanto, a média de idade ao parto entre os animais reagentes foi maior e estatisticamente significante e o peso das crias nascidas de mães reagentes com idade maior ou igual a 48 meses foi menor do que os animais nascidos de cabras não reagentes.
Show more [+] Less [-]Establishment of values of bone mineral density (BMD) from radius´s metaphysary and diaphysary regions in Poodle by Radiographic Optic Densitometry | Estabelecimento de valores de densidade mineral óssea (DMO) das regiões metafisária e diafisária do rádio em cães da raça Poodle por meio da Densitometria Óptica Radiográfica Full text
2005
Caterina Muramoto | Franklin de Almeida Sterman | Ana Carolina Brandão de Campos Fonseca Pinto
Radiographic Optic Densitometry was used to measure bone mineral content by radiographic images. This method contributes to the diagnosis of osteopenia, preventive estrategies associated to bone metabolism and monitoring of series of measurement of density to accompany pathologies, surgeries, therapies and. The optic density was measured by the image processing software, ImageLab; the applicability of this method was tested and canine reference values of bone mineral density (BMD) were compared to a standard aluminium scale radiographed with the bone. Correlations between the BMD values and the variables weigth, age, sex and width and thickness of the distal third of the 112 Poodles´s right radius-ulna were studied. It was observed that diaphysary BMD values were higher than metaphysary BMD values due to the differences between cancelous and compact bones. However, these differences in the BMD values were not observed in the younger group, up to about one year of age, probably due to the higher bone metabolism during the growing period in the metaphysary region. The average BMD values for each weight category showed that the best method to establish reference values was by weight and bone region. Average values of metaphysary region (M) and diaphysaries 1 (D1) and 2 (D2) were established in three different weight categories. For dogs up to 2.5kg in weight these values were BMD-M=2.55±0.30, BMD-D1=2.89±0.40 and BMD-D2=3.16±0.43mmAl; for dogs from 2.6 up to 5.0kg these values were BMD-M=3.16±0.43, BMD-D1=3.67±0.47 and BMD-D2=3.96±0.55mmAl and for dogs from 5.1 up to 7.5kg these values were BMD-M=3.72±0.50, BMD-D1=4.31±0.52 and BMD-D2=4.60±0.62mmAl. | A Densitometria Óptica Radiográfica permite aferir o conteúdo mineral do osso por meio de imagens radiográficas, contribuindo para o diagnóstico de osteopenia, monitoração seriada da densidade em resposta a enfermidades ósseas, a intervenções cirúrgicas e de terapia, e a estratégias preventivas relacionadas ao metabolismo ósseo. As densidades ópticas foram medidas por meio do software de processamento de imagens ImageLab, cuja aplicabilidade foi testada para determinação de valores de referência de DMO, comparando-os a uma escala padrão confeccionada em alumínio, radiografada junto ao osso. Correlacionou-se os valores de DMO com as variáveis peso, idade, sexo e largura e espessura do terço distal do rádio-ulna direito de 112 cães Poodle. Pela diferença na proporção de osso esponjoso e compacto entre as regiões metafisária e diafisária, os animais apresentaram a DMO da metáfise menor que a da diáfise, fato não observado no grupo de animais de até um ano de idade, devido ao alto metabolismo ósseo na região metafisária durante o período de crescimento. A análise dos valores médios de DMO obtidos para cada categoria avaliada, permitiu verificar que a classificação mais adequada para estabelecer valores de referência foi a por peso e região óssea. Assim, foram estabelecidos valores médios para a região metafisária (M) e para as diafisárias 1 (D1) e 2 (D2) em três categorias de peso. Para cães de até 2,5kg os valores definidos foram: DMO-M=2,55±0,30, DMO-D1=2,89±0,40 e DMO-D2=3,16±0,43mmAl; para os de 2,6 a 5,0kg foram: DMO-M=3,16±0,43, DMO-D1=3,67±0,47 e DMO-D2=3,96±0,55mmAl e para os de 5,1 a 7,5kg foram: DMO-M=3,72±0,50, DMO-D1=4,31±0,52 e DMO-D2=4,60±0,62mmAl.
Show more [+] Less [-]Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells | Detecção de citocinas no sítio de inoculação subcutânea de Leishmania (Leishmania) amazonensis em camundongos depletados de células Natural Killer Full text
2005
Márcia Dalastra Laurenti | Andres Örn | Idércio Luiz Sinhorini | Carlos Eduardo Pereira Corbett
<!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG /> </o:OfficeDocumentSettings> </xml><![endif]--> <p class="MsoNormal" style="text-align: justify;"><span style="mso-ansi-language: EN-US;" lang="EN-US">BALBI c mice depleted and non-depleted of Natural Killer (NK) cells were infected subcutaneously with 10<sup>7</sup> stationary phase promastigotes of Leishmania (Leishmania) amazonensis and samples were taken at 24 hours and 7 days after infection. In NK cell-depleted mice, the NK cytotoxic activity of spleen cells decreased at 7 days after infection and more parasites were found in the lesion. The NK cell populations were analyzed by immunohistochemistry in spleen cryosections. An increase of NK1.1<sup>+</sup> expression and a decrease of NK5E6<sup>+</sup> antigen expression was observed in NK cell-depleted mice compared to non-depleted mice. When the presence of IFN-g, IL-12 and IL-4 at the site of parasite inoculation was analyzed by immunohistochemistry, a large amount of cytokines was detected in K cell-depleted mice at 24 hours and 7 days after infection. In non-depleted mice, there was a small amount of IL-12 at 24 hours and of IL-4 at 7 days after infection. These data cells suggest that K cell depletion by 90Srresults in increased parasitism in the lesion. 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mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-fareast-language:EN-US;} </style> <![endif]--> | <!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG /> </o:OfficeDocumentSettings> </xml><![endif]--> <p class="MsoNormal" style="text-align: justify;">Camundongos BALB/ c depletados e não depletados em células Natural IVller (NK) foram infectados subcutaneamente com 10<sup>7</sup> promastigotas de Leishmania (Leishmania) amazonensis em fase estacionária de crescimento e amostras foram colhidas às 24 horas e 7 dias de infecção. Nos camundongos depletados em células NK, a atividade citotóxica NK de células esplênicas estava diminuída aos 7 dias de infecção e mais parasitos foram encontrados na lesão. Populações de células NK foram analisadas por imuno-histoquimica em cortes congelados de baço. Foi observado aumento na expressão de células NK1.1<sup>+ </sup>e diminuição na expressão do antígeno NK5E6<sup>+</sup> nos animais depletados em células NK comparados aos camundongos não depletados. A presença de IFN-g, IL-12 e IL-4, analisada por imunohistoquimica no sítio de inoculação dos parasitos, mostrou que maior quantidade de citocinas foram detectadas nos camundongos depletados em células NK às 24 horas e 7 dias depois da infecção. Nos camundongos não depletados, havia pequenas quantidades de IL-12 às 24 horas e de IL-4 aos 7 dias de infecção. Estes dados sugerem que a depleção de células NK por <sup>90</sup>Sr resulta em aumento de parasitismo na lesão. O aumento na expressão de células NK1.1<sup>+</sup>, as quais produzem principalmente IL-4, pode representar um dos mecanismos que colaborariam para a progressão da infecção.</p> <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>PT-BR</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:SplitPgBreakAndParaMark /> <w:EnableOpenTypeKerning /> <w:DontFlipMirrorIndents /> <w:OverrideTableStyleHps /> </w:Compatibility> 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Show more [+] Less [-]Morphological aspects of the uterine tube in agouti (Dasyprocta aguti, Mammalia: Rodentia) | Aspectos morfológicos da tuba uterina de cutias (Dasyprocta aguti, Mammalia: Rodentia) Full text
2005
Eunice Anita de Moura Fortes | Maria Acelina Martins de Carvalho | Mônica Marcos de Almeida | Aírton Mendes Conde Júnior | Nádia Expedita de Almeida Cruz | Antônio Chaves de Assis-Neto
It was analysed the uterine tube structure in Agouti. We have used three adult females, caming from Wild Animals Preservation and Study Nucleous of "Universidade Federal do Piauí". Soon after anaesthesia and euthanasia we got collected uterine tube fragments from cranial, medial and caudal regions. The cuts of 3 to 5m were stained with HE, and analysed and photographed with a light microscope. We could observed that the uterine tubes have three distincts stratums: mucous membrane, muscular and serous with histologicals differences that characterize the infundibulum, ampulla and isthmus regions. The uterine tube light is irregular, on cranial and medium portions. It is bigger and irregular than caudal portion. The mucous membrane is coat with simple columnar epithelium with ciliar and nonciliar cells. The own lamina, formed of slack conjunctive tissue, without glands, on ampulla region, become small and we can observe many cells. On isthmus mucous we observe ephithelial tissue with low cells. The muscular, constituted of smooth fibres, that is observed like a circular and longitudinal stratum, and the serous stratum full of vessels. Histologically, the uterine tube in Agouti is similar to the others domestic and wild animals. | Foi analisada a estrutura da tuba uterina de cutias utilizando-se três fêmeas adultas, oriundas do Núcleo de Estudos e Preservação de Animais Silvestres da Universidade Federal do Piauí. Após anestesia, foi realizada a eutanásia dos animais, em seguida, obtidos fragmentos da tuba uterina correspondentes às regiões cranial, média e caudal. Cortes de 3 a 5m de espessura foram corados pela Hematoxilina/Eosina e analisados e fotografados ao microscópio de luz. Foi evidenciado que, a parede da tuba uterina da cutia apresenta três camadas histologicamente distintas: mucosa, muscular e serosa, caracterizando as regiões infundíbulo, ampola e istmo. A mucosa, revestida por tecido epitelial simples colunar apresentando células ciliadas e não ciliadas, e, com grande quantidade de pregas em diferentes níveis. No epitélio de revestimento da mucosa do istmo, foram observadas áreas com células variando de cúbicas a pavimentosas. A luz do órgão, nas porções cranial e média, mostra-se bem maior e mais irregular que na porção caudal. A lâmina própria, constituída de tecido conjuntivo frouxo, desprovida de glândulas, e na região da ampola, torna-se mais estreita e bastante celularizada. A camada muscular, formada por fibras musculares lisas dispostas em uma subcamada circular interna e outra longitudinal externa. A serosa mostra-se bastante vascularizada. As tubas uterinas da cutia guardam muita semelhança com as dos demais mamíferos quanto aos aspectos microscópicos.
Show more [+] Less [-]Origem do plexo braquial do macaco Cebus apella | Origin of the Brachial Plexus of the monkey Cebus apella Full text
2005
Adriana Rodrigues Ribeiro | Irvênia Luiza de Santis Prada | Zenon Silva | Roseâmely Angélica de Carvalho Barros | Daniela Cristina de Oliveira Silva
O Cebus apella, animal das matas do continente Sul-americano, distribui-se geograficamente por quase todo o Brasil. Apresenta satisfatória adaptação ao cativeiro, condição em que se reproduz com facilidade. Assim, focalizamos o Cebus apella, na origem de seu plexo braquial. Nosso objetivo é dar seqüência ao conhecimento de sua anatomia, visando também ao fornecimento de subsídios para interpretações anátomo-funcionais, comparativamente ao homem e aos animais domésticos. Foram utilizados 20 animais, sendo 10 machos e 10 fêmeas, adultos, pertencentes ao acervo de pesquisas da Universidade Federal de Uberlândia, Uberlândia - MG. Preparamos as peças anatômicas segundo a metodologia usual em estudos anatômicos. Dos espécimes dissecados, o plexo braquial do Cebus apella está constituído por raízes de C5 a T1 em 55,00 ±± 11,12%, de C5 a T2 em 25,00 ±± 9,68%, de C4 a T1 em 15,00 ±± 7,98% e de C4 a T2 em 5,00 ±± 4,87%. Observou-se a ocorrência de pré e de pós-fixação do plexo, bem como o seu deslocamento cranial e caudal. Em conclusão, o plexo braquial do Cebus apella está constituído por raízes de C5 a T1. | The monkey Cebus apella, native to South American forests, is geographically distributed through the Brazilian land and presents satisfactory adaptation to the captive life showing great capacity of reproduction. Thus, we have described the origin of the brachial plexus in the monkey Cebus apella. The aim of this study was to add information for morphofunctional and comparative studies focusing on Cebus apella, humans and domestic animals. Twenty adult animals, 10 males and 10 females, belonging to the collection of Anatomy Laboratory of the Federal University of Uberlândia were obtained and prepared through fixation and dissection. In the dissected specimens, the brachial plexus of Cebus apella was comprised of nerve roots from C5 to T1 (55,00 ±± 11,12%), from C5 to T2 (25,00 ±± 9,68%), from C4 to T1 (15,00 ±± 7,98%) and from C4 to T2 (5,00 ±± 4,87%). In addition, the occurrence of pre- and post- fixation of the plexus as well as its cranial and caudal extent have been discussed. In conclusion, the brachial plexus of Cebus apella is constituted by nerve roots from C5 to T1.
Show more [+] Less [-]Comparação entre duas técnicas de biópsia renal guiadas por laparoscopia em eqüinos | Comparison of two laparoscopic guided renal biopsy techniques in horses Full text
2005
Alexandre de Faria Tabet | Luís Cláudio Lopes Correia da Silva | Idércio Luis Shinhorini | André Luis do Valle de Zoppa
Renal biopsy is a useful diagnostic technique for the identification to the type of lesion, severity of the disease and its evolution. The high incidence of renal lesions in horses, which usually are a consequence of digestive and respiratory diseases and are frequently associated with endotoxemia, prompted us to study the laparoscopic technique for renal biopsy compair the use of needle and forceps. For the purpose of this study ten helthy horses were randomly chosen and divided into two grups of five animals each: in one group laparoscopic guided renal biops was performed using tru-cut needles and in the other group using 5mm laparoscopic forceps. Procedures were carried out with the animals in standing position. Animals were given a sedative analgesic combination and local anesthetic was infiltrated in two specific points on the left flank. One point was used to introduce the trocar and laparoscopic, the other was used to introduce the forceps or the needle. The techniques were compared as regards safety, intensity of bleeding, time for execution and significance of the sample obtained. In all of the procedures, were possible to inspect the abdominal cavity, and chose the place to collect samples, without acidents or complications. The histological analysis of the sample showed a higher percentage of adequate biopsies in the group where laparoscopic forceps were used that permit us to viable both of them with preference for the forcps use. | A biópsia renal é técnica de diagnóstico útil para identificação do tipo e severidade e acompanhamento da evolução da doença renal. A alta incidência de lesões renais em eqüinos, geralmente em conseqüência de distúrbios digestivos ou respiratórios, freqüentemente associados a endotoxemia, nos motivou a estudar a técnica de biópsia renal guiada por laparoscopia, comparando o uso de agulha com pinça de biópsia. Foram utilizados 10 eqüinos hígidos, distribuídos aleatoriamente em dois grupos. Em cinco animais foi realizada biópsia renal com agulha descartável do tipo tru-cut e nos outros cinco com pinça de biópsia de 5mm, sendo que o procedimento foi guiado em ambos os grupos com auxílio da laparoscopia. Os animais permaneceram em estação durante a biópsia, que foi realizada mediante sedação e analgesia e bloqueio anestésico local em 2 pontos no flanco esquerdo, sendo um para introdução do trocarte e laparoscópio e outro para introdução da agulha ou pinça. As técnicas foram comparadas quanto à segurança, intensidade do sangramentro, tempo de execução e significância da amostra colhida. Em todos os procedimentos, foi possível a inspeção da cavidade peritoneal e escolha do local de colheita do material, com ausência de acidentes ou complicações o que nos permitiu validar ambas as técnicas. A análise histológica dos fragmentos demonstrou maior porcentagem de biópsias consideradas adequadas no grupo em que foi utilizada pinça laparoscópica o que nos leva a indicar preferencialmente seu uso.
Show more [+] Less [-]Histological evaluation of goat's hair at different ages | Avaliação histológica de peles de caprinos em idades diferenciadas Full text
2005
Maria Auxiliadora de B. L. Dal Monte | Roberto Germano Costa | Marinei Grotta | Gerson Bragagnoli | Manuel Antônio Chagas Jacinto | Ariosvaldo Nunes de Medeiros
The objective of theis research was to analyze the skin of goats, identifying the structural differences in the cell level. It was used 16 animals, males, crossbred Alpine, using 4 animals in each slaughter, at the age of 180 days and older, used intervals of 45 days between each slaughter. After flaying the animals, it was removed samples of the skin from dorsal, palette, ventral, hind and lateral regions. These samples were placed in a Bouin solution an analyzed at cellular level, according to the paraffin inclusion routine. The 5 mm cuts were photomicrographed and analyzed. The goats skin that were 180 days old shown a thicker thermostatic layer of the skin, consequently with a greater density of follicles and glands. With age the reticular layer develops leading to the sheaves of fibers of the collagen. | Objetivando-se analisar a pele de caprinos, identificando as diferenças estruturais histológicas, utilizou-se 16 animais machos mestiços da raça Alpina, com 4 animais em cada abate a partir dos 180 dias com intervalos entre cada abate de 45 dias. Após o sacrifício foram retiradas amostras de pele das regiões dorsal, paleta, ventre, anca e lateral. Estas amostras foram fixadas em Bouin e processadas histologicamente segundo rotina de inclusão em parafina. Os cortes de 5 mm foram analisados e fotomicrografados. A pele dos caprinos, com 180 dias de vida, mostrou-se com maior espessura da camada termostática da derme, consequentemente com grande densidade de folículos e glândulas. Com o aumento da idade os feixes de fibras do colágeno se desenvolveram aumentando a espessura da camada reticular.
Show more [+] Less [-]Características estruturais e ultraestruturais da espermatogênese do cascudo-cinza Liposarcus anisitsi (Holmberg, 1893) (Teleostei, Siluriformes) | Structural and ultrastructural characteristics of the spermatogenesis of the grey armored catfish Liposarcus anisitsi (Holmberg, 1893) (Teleostei, Siluriformes) Full text
2005
Claudinei da Cruz | Antônio Marcos Orsi | Karina Simões | Carlos Alberto Vicentini
Liposarcus anisitsi is a freshwater teleost from Siluriform order with external fertilization that shows cystic spermatogenesis process with spermatogonial unrestricted lobular structure of the testes. In this species, isolated or intracystic primary spermatogonia were noted as the ¨stem cells¨ of the spermatogenetic lineage. Thus, as consequence of the cystic spermatogenesis process of Liposarcus anisitsi all the three germinative cells are observed inside specific spermatogenetic cysts, since the primary and secondary spermatogonias until the spermatocytes and early and late spermatids. Spermatogonia show spherical shape with central nuclei and great amount of round mitochondria and ER profiles mainly of rough type in their cytoplasm. Primary spermatocytes have inter-relations through cytoplasmic bridges and are clearly identified by presence of synaptonemic complexes inside the nuclei. Early spermatids beginning maturational phases, as well as the late spermatids, tend to exhibit spherical nuclei, variable chromatin pattern with progressive condensation during the end steps of the spermiogenesis. In this end steps, the late spermatids show the nuclear fossa formed to the fixation of the flagella. The spermatozoon of Liposarcus anisitsi shows round head, round nuclei, very dense chromatin, lack of acrosoma, short middle piece and long tail (principal and end pieces of the flagellum). | Liposarcus anisitsi é um teleósteo de água doce da ordem Siluriforme, com fertilização externa, possui um processo de espematogênese cística com estrutura espermatogonial lobular irrestrita dos testículos. Nesta espécie, a espermatogônia primária intracística ou isolada é considerada como a "stem cell" da linhagem espermatogenética. Então, como consequência do processo da espermatogênese cística de Liposarcus anisitsi todas as três células germinativas são observadas no interior de cistos espermatogenéticos específicos, desde as espermatogônias primárias e secundárias até os espermatócitos e espermátides jovens e maduras. A espermatogônia possui forma esférica, com núcleo central e grande quantidade de mitocôndrias redondas e retículo endoplasmático, principalmente do tipo rugoso, em seu citoplasma. Os espermatócitos primários se inter-relacionam através de pontes citoplasmáticas e são claramente identificados pela presença de complexos sinaptonêmicos no interior de seus núcleos. Espermátides jovens no início da fase maturacional, tanto quanto as espermátides maduras tendem a exibir núcleos esféricos, um modelo variável de progressiva condensação cromatínica durante o estágio final da espermiogênese. Nestes estágios finais, as espermátides maduras mostra uma fossa nuclear formada para a fixação do flagelo. O espermatozóide de Liposarcus anisitsi possui cabeça redonda, núcleo redondo, cromatina densa, ausência de acrossoma, curta peça intermediária e longa cauda (peças principal e terminal do flagelo).
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