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Detection of testosterone residues in farm fish tissue
2009
H. R. Abdel-Dayem | Hanaa M. Soltan | G. Naser
A total of one hundred samples of marketed fish farm; 25each of tilapia (Oreochromis niloticus), Mugil cephalus, Carp (Silver) and fry; were collected from different localities at Al- Behera and Kafr El-Shakh Provinces. The samples were examined for detection of testosterone hormone by Thin Layer chromatography (TLC) as well as it was quantitatively assayed by Radio-Immuno-Assay (RIA). The obtained result revealed that the testosterone residues were detected in flesh of farm fish of Tilapia (Oreochromis nilotocus) and Carp (Silver), each constituting 24% and 4 %; respectively but it could be failed to detect in each of Mugil cephalus and fry. The hormonal residues of testosterone was ranged from 3.25 to 34.9 ng/g with mean value 4.22±1.1 ng/g in positive samples of Tilapia, while only one sample of Carp showed 22.0 ng/g, Mugil cephalus and Fry showed no detectable level of hormonal residues. The public health significance of detectable levels was discussed as well as the recommendations to avoid health hazards from such fish were mentioned.
Show more [+] Less [-]African swine fever : transboundary diseases
2009
M-L. Penrith
African swine fever (ASF) is a devastating haemorrhagic fever of pigs that causes up to 100 % mortality, for which there is no vaccine. It is caused by a unique DNA virus that is maintained in an ancient cycle between warthogs and argasid ticks, making it the only known DNA arbovirus. ASF has a high potential for transboundary spread, and has twice been transported from Africa to other continents - Europe and subsequently the Caribbean and Brazil (1957, 1959) and the Caucasus (2007). It is also a devastating constraint for pig production in Africa. Research at Onderstepoort Veterinary Institute has made and is making important contributions to knowledge of this disease, focusing on the cycle in warthogs and tampans and transmission from that cycle to domestic pigs, resistance to its effects in domestic pigs, and the molecular genetic characterisation and epidemiology of the virus.
Show more [+] Less [-]Molasses as a possible cause of an ''endocrine disruptive syndrome'' in calves
2009
M.S. Masgoret | C.J. Botha | J.G. Myburgh | T.W. Naude | L. Prozesky | V. Naidoo | J.H. van Wyk | E.J. Pool | G.E. Swan
During the mid 1990s a potentially serious, chronic syndrome was reported in well-managed beef and dairy herds from unrelated parts of South Africa. Farmers reported that it manifested as various combinations of decreased production, decreased weaning masses, apparent immune breakdown in previously immunocompetent animals, increased reproductive disorders, various mineral imbalances in non-deficient areas and goitre, noticeable as enlarged thyroid glands. The farmers associated this syndrome with certain batches of sugar cane molasses and molasses-based products. The syndrome was reminiscent of an ''endocrine disruptive syndrome''. The objective of this study was to evaluate the suspected endocrine disruptive effect of molasses included in cattle feed. Using existing in vitro assays, four batches of molasses syrup were screened for possible inclusion in a calf feeding trial. Two batches were selected for the trial. Thirty-two, 4- to 6-week-old, weaned Holstein bull calves were included in the single phase, three treatment, parallel design experiment. In two of the groups of calves, two different batches of molasses were included in their rations respectively. The control group was fed a ration to which no molasses was added, but which was balanced for energy and mineral content. The mass gain of the calves was recorded over the 6-month study period. The calves were clinically examined every week and clinical pathology parameters, immune responses and endocrine effects were regularly evaluated. Even though endocrine disrupting effects were detected with the in vitro screening assays, these could not be reproduced in the calves in the experiment. The two batches of molasses utilized in the calf feeding trial did not induce major differences in any of the parameters measured, with the exception of a lower mass gain in one of the molasses-fed groups (Group 1), which tended towards significance. The results of the study indicate that the two batches of molasses had no endocrine disruptive or immunosuppressive effects in calves.
Show more [+] Less [-]Persistence mechanisms in tick-borne diseases : tick-borne diseases
2009
A.F. Barbet
The use of new, highly sensitive diagnostic methods has revealed persistent infections to be a common feature of different tick-borne diseases, such as babesiosis, anaplasmosis and heartwater. Antigenic variation can contribute to disease persistence through the continual elaboration of new surface structures, and we know in several instances how this is achieved. Known or suspected mechanisms of persistence in babesial parasites include cytoadhesion and rapid variation of the adhesive ligand in Babesia bovis and genetic diversity in several merozoite stage proteins of different Babesia spp. In Anaplasma, extensive variation in the pfam01617 gene family accompanies cycling of organism levels in chronic infection. One result from the pioneering research at Onderstepoort is the definition of a related polymorphic gene family that is likely involved in immunity against heartwater disease. We are beginning to understand the sizes of the antigenic repertoires and full definition is close, with the possibility of applying simultaneous high-throughput sequencing to the order of 1 000 small genomes. We also, for the first time, can consider modifying these genomes and looking at effects on persistence and virulence. However, important biological questions remain unanswered; for example, why we are seeing a new emerging Anaplasma infection of humans and is infection of endothelial cells by Anaplasma significant to persistence in vivo.
Show more [+] Less [-]In vivo and in vitro study on the effect of Bacillus subtilis and its byproducts on Clostridium perfringens Type A
2009
I. A. Radwan | Eman R. Hassan
The objectives of this study were to screen the possible effects of B. subtilis and its soluble byproducts against C. perfringens, a causative agent of necrotic enteritis (NE) in chickens. The use of B. subtilis strain was found to be inactive in vitro against C. perfringens but its cell filtrate byproducts produced after growth of B. subtilis at 37°C with medium pH adjusted at 5.0, having inhibitory effect in the form of inhibitory zones; measured inhibition of 12 mm. The produced anticlostridial factor was not affected by heat treatment at 70, 100 or 121°C for 15 minutes. The study recorded the responses of broiler chickens to oral administration of C. perfringens and the possible antagonistic effects of B. subtilis and its cell-free filtrate byproducts in vivo. Results revealed that B. subtilis decreased the severity of intestinal necrotic lesions produced after oral inoculation of C. perfringens and the suggested anticlostridial effect was more clear when the cell- free filtrate produced by growing B. subtilis at 37°C with pH 5.0 was added to feed at a dose of 20 ml/kg of ration.
Show more [+] Less [-]Impact of age at first calving on reproduction, lactation, postpartum disorders and longevity in Holsteins under Egyptian circumstances
2009
M. M. Hussein | A. A. A. El Agawany
A retrospective study including reproductive and productive data of 1587 primiparous Holstein cows covering a period of eight years. The reproductive data ( interval to 1st service, service period, days open, number of inseminations per conception and calving interval ) and the productive data ( total milk yield, average daily milk yield and 305 day milk yield) were studied as a function of age at first calving and postpartum disorders. Furthermore, other data including life span longevity, number of parities, the cumulative productive days and the average calving interval were also studied as a function of age at first calving. Cows were classified according to the age at first calving into young age calvers (< 24 months), mid age calvers (> 24-28 months) and old age calvers (> 28 months). The obtained data were statistically analyzed, tabulated and discussed. The overall mean age at first calving was 26.68 months in Holstein cows under Egyptian circumstances. Most of first calving (90%) were recorded in cows less than 30 month of age. The results indicated that, the young age calvers tend to have prolonged interval to 1st service, days open and calving interval and the reverse was correct in old age calvers. The postpartum disorders had significantly detrimental effects on the reproductive performance among the different age groups. Young age calvers had a higher incidence of postpartum disorders than those of mid - age calvers and much higher than had the old age group. The postpartum disorders were associated with high milk production specially in mid and old age calvers which may indicate the high predisposition of superior producer cows to postpartum disorders. Life span, longevity, number of parities and the cumulative production days were found to be increased as a function of age at first calving. Meanwhile, the average calving interval of the young age calvers was longer than the other two age groups. Finally, it was recommended to avoid insemination of maiden Holstein heifers less than 17 month of age specially those having superior productive traits.
Show more [+] Less [-]Pharmacokinetic profile and some pharmacodynamic aspects of cefquinome in chickens
2009
A. A. M. El-Gendy | M. A. Tohamy | Abeer M. Radi
The pharmacokinetic profile and some pharmacodynamic aspects of cefquinome were studied after intramuscular (IM) and subcutaneous (SC) administration of a single dose of 2 mg kg-1 b.wt. in chickens. Tissue distribution and residues of cefquinome after repeated IM injection for 5 consecutive days were also estimated. Cefquinome was rapidly absorbed after IM and SC injection as indicated by short half-lives of absorption (t0.s(ab)) of 0.170 and 0.262 h., respectively, while the elimination half-lives (t05(el)) were 3.428 and 25.023 h., respectively. Repeated IM doses of cefquinome (2 mg kg-1 b.wt., once daily) for 5 consecutive days caused no change in serum enzyme activities of ALT and AST, but induced significant increase in serum uric acid concentration after 72 to 120 hours of administration. The withdrawal time of cefquinome from tissue of chickens is 5 days following the last dose. Cefquinome has a wide spectrum of activity against Esherchia coli, Proteus mirabilis and Pseudomonas aeroginosa.
Show more [+] Less [-]Identification and partial sequencing of a crocodile poxvirus associated with deeply penetrating skin lesions in farmed Nile crocodiles, Crocodylus niloticus
2009
F.W. Huchzermeyer | D.B. Wallace | J.F. Putterill | G.H. Gerdes
When large numbers of crocodile skins were downgraded because of the presence of small pin pricklike holes, collapsed epidermal cysts were found deep in the dermis of juvenile crocodiles while forming cysts were observed in hatchlings. Histopathology of these forming cysts showed the presence of intracytoplasmic inclusions in proliferating and ballooning epidermal cells. Pox virions were seen in electron microscope preparations made from the scabs of such early lesions. The partial sequencing of virus material from scrapings of these lesions and comparison of it with the published sequence of crocodile poxvirus showed the virus associated with the deep lesions to be closely related, but different. To differentiate between the two forms of crocodile pox infection it is suggested that the previously known form should be called ''classical crocodile pox'' and the newly discovered form ''atypical crocodile pox''. The application of strict hygiene measures brought about a decline in the percentage of downgraded skins.
Show more [+] Less [-]Studies on Edwardsiella tarda infection in catfish and Tilapia nilotica
2009
Heidy Abo El-Yazeed | Mai D. Ibrahem
Edwardsiella tarda was recovered from cultured freshwater catfish (Claries gariepinus) with a prevalence rate of 0.42% in summer season. Experimental infectivity studies of E. tarda in Catfish and Tilapia nilotica were done using the intraperitoneal (I/P) and intramuscular (I/M) routes. Experimentally infected Catfish with a dose of 0.3 ml from E. tarda bacterial suspension of 108 CFU/ml manifested pathognomonic clinical signs and postmortem lesions than the experimentally infected Tilapia nilotica. Application of PCR technique for rapid detection of E. tarda infection in the experimentally infected fishes gave positive reaction in 4th day post infection in catfish and the 7th day post infection in Tilapia nilotica.
Show more [+] Less [-]The future of private veterinary practice in South Africa : animal health management in the 21st
2009
C. Marwick
Private practice became the major vocation of local veterinarians from about 1945. Soga was probably the first qualified South African veterinarian to practice privately on a limited scale in the 1800s. Jack Boswell was the first locally qualified (in 1934) veterinarian to go directly into practice without first working for the government and is regarded as the father of private practice in South Africa. Boet Bekker worked for the government before setting up a successful practice in 1935.
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