Citrobacter freundii strains, like other representatives of the same genus, are often described as aetiological factors of diseases in humans and animals, including farmed and ornamental fish. The aim of the study was the isolation and identification of C. freundii from ornamental fish in Poland and investigation of the virulence factors and antibiotic resistance among the isolates.

Piglets are vulnerable to stress during weaning because of changes in the feeding environment, nutrients, and other growth-impacting conditions. In this study, stress injury was modelled by continuous intraperitoneal injection of lipopolysaccharide (LPS) and was used to investigate the dynamics of antioxidant indices and immunoinflammatory factors in the piglet thymus.

Staphylococci are still a challenge in veterinary medicine, as they are one of the aetiological factors causing clinical and subclinical mastitis in small ruminants. The aim of the study was to analyse the occurrence of staphylococci in milk obtained from Świniarka (SW) and Uhruska (UHR) sheep and to characterise their drug resistance and virulence.

Food business incubation centre (FBIC) under the Tamil Nadu Veterinary and Animal Sciences University established during 2014 hadfacilitated the farmers, students and entrepreneurs in terms of training, consultancy, external project and incubation services. To evaluate the performance of the Food Business Incubation Centre on incubation services, data were collected for a period of 10 years from 2014-15 to 2023-24 and analyzed. The study also included the available data from the financial year 2018-19 to 2023-24 to assess the types ofvisitors and their purpose of visit to the incubation centre and the data were analyzed. It was concluded that majority of the incubatees utilized the FBIC for the incubation services in the area of dairy processing. Totally 14 members successfully graduated of which 50 % of them were female. The highest number of visits was recorded during the financial year 2022-2023 and the maximum of the visitor category was students from schools and colleges and only 9.7 % of the visitors were business aspirants out of the total visitors during the study period.

The present investigation was undertaken to study the occurrence of enterotoxigenic B. cereus from 200 samples including 100 raw milk, 50pasteurized milk and 50 ice cream samples sold in Puducherry. The results revealed that 68 samples (34 %) belonged to B. cereus group which included 36 from raw milk (36%), 9 from pasteurized milk (18%) and 23 from ice cream samples (46 %). On PCR analysis, 64 out of 68 isolates showed the presence of gyrB gene which differentiates B. cereus from B. cereus group isolates. Multiplex PCR revealed the presence of four enterotoxigenic genes hblA, nheA, cytK and entF Min 32.35 %, 77.94 %>, 54.41 % and 94.12% of the 64 isolates. On biofilm production assay, out of 64 enterotoxigenic B. cereus isolates, 60 (93.75 %) isolates showed the ability to form biofilm in which 13 (20.31 %) were strong, 21 (32.81 %) were moderate and26 (40.63 %) were weak biofilm producers. All the isolates were resistant to ampicillin and penicillin G and sensitive to ciprofloxacin, gentamicin, vancomycin, streptomycin and norfloxacin. The current study directs the need for proper hygienic measures during collection, production, processing and storage of milk to reduce the contamination level. It also indicates the need for surveillance and action to reduce the contamination of enterotoxigenic B. cereus in milk and milk products in Puducherry.

A Dalmatian dog -was brought to the Referral Veterinary Polyclinic, ICAR- Indian Veterinary Research Institute, Izatnagar with a complaint of erythematous lesions, alopecia and intense itching and had been treated for a long time. Atopic dermatitis was diagnosed based on the history and the exclusion of other infectious agents as potential causes of the dermatitis. The dog was treated with oclacitinib, antibacterial, fatty acids and antihistaminic medications and showed remarkable improvement after therapy.

Organophosphates (OP) and carbamates are widely utilized insecticides, but they also pose significant risks as sources ofintoxicationfor both humans and animals. A male non-descript dog, seven years old was presented with a chronic respiratory distress, dysphonia, exercise intolerance, and neurologic signs to Veterinary Clinical Complex, VCRI, Orathanadu on April 2023. Physical examination revealed elevated rectal temperature and congested mucous membrane with elevated heart beat and respiratory rate. Thoracic auscultation revealed muffled heart sound. The dog's medical history indicated a potential case of organophosphate poisoning. Treatment involved administering atropine sulfate, normal saline, ceftriaxone, and furosemide. However, throughout the treatment process, the dog continued to experience upper respiratory stridor and dyspnoea. Clinical examination and radiographic observations confirmed the diagnosis of laryngeal paralysis. This case highlights the occurrence of laryngeal paralysis polyneuropathy complex in a canine affected by organophosphate poisoning.

In India, small and marginal farmers, comprising 85% of the farming community, are pivotal to the rural economy. Addressing the rising demands amidst agricultural resource scarcity requires a holistic approach like Integrated Farming System (IFS). This method combines crop production, livestock, fishery, waste management, and poultry production synergistically. IFS boosts productivity, utilizes resources efficiently, and enhances food production, stabilizing income and ensuring nutritional security. A study in Karaikal district, Union Territory of Puducherry, which was an agricultural land and the entire farm revealed a benefit­cost ratio of 1.75, demonstrating IFS's potential in overcoming population growth and resource challenges.

Rational drug design, is the inventive process of finding new medications based on knowledge of the biological target. Drug design involves the design of small molecules that are complementary in shape and charge to the bimolecular target to which they interact and therefore will bind to it. In the experiment based approach, drugs are discovered through trial and error. With high R&D cost and consumption, computational drug discovery helps scientists gain insight into drug receptor interactions and reduce time and cost. Scientists can predict whether the molecule will succeed or fail in the market. Currently, the process of drug designing increasingly relies on computer modeling techniques. This type of modeling is often referred to as computer-aided drug design. In computational drug discovery, different computational tools, methods, and software are used to simulate drug receptor interactions. Using computational drug discovery helps scientists gain insight into drug receptor interactions with less time and cost.

Food bait preferences of house flies were evaluated using olfactometry bioassays. A single cage dual port glass olfactometer was used to assess preferences of house flies towards sugar, fish meal and molasses in no choice and choice bioassays. Flies were allowed into olfactometer individually, observed for five minutes. Time spent in bait port and decision chamber, number of entries into test/control port and port selected five minutes post-exposure were recorded. In the second study in a four arm acrylic olfactometer, three food baits viz., high fish meal low molasses cake, low fish meal high molasses cake and mango essence were tested In the glass olfactometer, flies showed positive response towards food baits compared to control in no choice bioassay. In choice bioassay, flies preferred fish meal than both sugar and molasses and molasses was preferred over sugar. In four arm acrylic olfactometer bioassay, 43.87, 30.96 and 25.16 per cent flies were attracted to high fish meal low molasses cake, low fish meal high molasses cake and mango essence, respectively. Results revealed that molasses and fish meal based baits can be used in housefly traps. Olfactometry bioassays were found effective in bait preference studies of house flies.