In the conditions of the Republic of Belarus there were analyzed indices of impregnating ability and efficiency of stud bull mothers of various genotypes on a kappa-casein gene (CSN3). As a result of genotyping of kappa-casein gene by DNA-diagnostics method it is revealed three genotypes - CSN3АА, CSN3AB, CSN3BB. Frequency of occurrence of homozygous genotype CSN3AA at stud bulls - 87,3%; heterozygotic CSN3AB - 20,5%; homozygous CSN3BB - 1,2%. Statistically authentic difference between these indices depending on a genotype it is not established. Thus, authentic connection between indices of impregnating ability and efficiency of stud bull mothers and their genotype on a locus of a kappa-casein gene that specifies in absence of influence of the last on the given indices is not established and gives the chance to conduct selection on gene CSN3 without decrease in breeding value of stud bulls on reproductive qualities.

In the conditions of the Republic of Belarus there was studied the interrelation between content of major nutrient elements (calcium and phosphorous) and trace elements (iron, copper, cobalt, and manganese) in forages and blood of first-calf cows at the first and last stages of lactation. Also, there were developed formulas of mixed forages and a premix for these animals. The studies were realized at the Agricultural Production Co-operative Olgovskoe of Vitebsk region (Belarus). Research of analysis of forages and state of mineral metabolismat heifers made it possible to draw following conclusions: in cow rations there was marked the deficiency of vitamin D, as well as some trace elements (cobalt and manganese) in the conditions of calcium and phosphorus excess. In spite of a high content in a calcium in diets, it was noted than in blood plasma the concentration of this element was below the standard for the majority of animals. Lack of cobalt and manganese of whole blood of cows was connected with a low level of these elements in cow diets. The surplus contents of copper in forages and blood of animals against the background low activity of ceruloplasmin the weakened antioxidant defense of heifer cows. For preventive treatment of possible hypo trace element deficiencies and metabolic disorders of heifer cows at the final stage of lactation there can be recommended the correcting of cow diets with inclusion into their composition of the developed for compound of mixed fodder and premix.

On the basis of the spent research high enough preventive efficiency of Ehrakond application, low intensive laser radiation together with a constant magnetic field is defined that allows to recommend them for wide application in the program of immunocorrection of an organism of agricultural animals. At the first stage of the research in immune deficiency when in blood of newborn calves almost there are no antibodies, influence on biologically active points of an udder of cows (intensity 10 mW/square cm, expositions of seconds) has made positive impact on increase of immune properties of colostric milk. Use of immunostimulants Ehrakond also has increased immunocompetent properties of cow milk (7,5 mg on 1 kg of body weight once a day within 15 days). At feeding of colostrum the physiological condition, growth and development of calves have considerably improved. In the second (when colostric protection factors in an organism decline, and own organism forms them insufficiently) and the third (decrease in immune reactance at changes in feeding and the maintenance of calves) immune deficiency, application of immunostimulants Ehrakond and influence of low intensive laser radiation together with a constant magnetic field on reflexogenic zones on a young growth organism has made positive impact indices of natural resistance of calves. It has led to activization of factors of natural nonspecific protection of an organism that has considerably improved a physiological condition and has increased natural immunity of an organism to diseases. However, the best results on natural resistance, efficiency and safeties of calves have been reached at an irradiation at the BAP initial stage of a mammary gland at cows by low intensive laser radiation together with a constant magnetic field, with the subsequent bottle-feeding of the irradiated colostrum to newborn calves; in 10 days of a life to calves the preparation Ehrakond was entered. | На основании проведенных исследований определена достаточно высокая профилактическая эффективность применения Эраконда, низкоинтенсивного лазерного излучения совместно с постоянным магнитным полем, что позволяет рекомендовать их для широкого применения в программе иммунокоррекции организма сельскохозяйственных животных. На первом этапе исследований в период иммунного дефицита, когда в крови новорожденных телят почти отсутствуют иммуноглобулины, воздействие на биологически активные точки вымени коров (интенсивность 10 мВт/см2, экспозиции секунд) оказало положительное влияние на повышение иммунных свойств колострального молока. Использование иммуностимулятора Эраконд также повысило иммунокомпетентные свойства молока коров (7,5 мг на 1 кг живой массы один раз в сутки в течение 15 дней). При скармливании колострального молозива значительно улучшились физиологическое состояние, рост и развитие телят. В период второго (когда колостральные факторы защиты в организме угасают, а собственный организм образует их недостаточно) и третьего (снижение иммунной реактивности при изменениях в кормлении, содержании телят) иммунного дефицита, применение иммуностимулятора Эраконд и воздействие низкоинтенсивного лазерного излучения совместно с постоянным магнитным полем на рефлексогенные зоны на теле молодняка оказало положительное влияние показатели естественной резистентности телят. Это привело к активизации факторов естественной неспецифической защиты организма, что значительно улучшило физиологическое состояние и повысило естественную невосприимчивость организма к болезням. Однако, наилучшие результаты по естественной резистентности, продуктивности и, сохранности телят были достигнуты при облучении на начальном этапе БАТ молочной железы у коров низкоинтенсивным лазерным излучением совместно с постоянным магнитным полем, с последующим выпаиванием облученного молозива новорожденным телятам; через 10 дней жизни телятам вводили препарат Эраконд.

In the conditions of the Republic of Belarus as a result of task-specific selection and breeding work there was developed and approbated Berezinsky breed type of Belarusian meaty breed. Sows of the studied breed type were notable for high indices of reproductive signs: the average multiple pregnancy in three breeding swine herd was 11,1 pigs per farrowing; milkability - 55 kg, litter size at weaning stage at 35-day age - 87,1 kg. The predominancy over the forecast exponent indices made 4,7-5,7%. In course of the study there were analyzed the forecasted indexes of the basic breeding signs of Berezinsky breed type of Belarusian meaty breed for 2005-2010; productivity indices of the experimental sows; coefficient of variations of sow productivity.

In the conditions of the Republic of Belarus there was studied the influence of various doses of dolomite on productivity, egg quality and a mineral metabolism of laying hens. The research was realized by an example of laying hens of Highsex Brown. The experimental hens at the age of 250 days old were divided into 4 groups on 60 heads in each by means of analog method. Laying hens of the first (control) group were fed with a basic diet including 5% of a soya cake (cockle-shell), and hens of the second, third and fourth (experimental) groups instead of a soya cake were fed with a mixed fodder with addition of a mineral additive of dolomite. Hens of the second group were fed with the additive at a rate of 2%, the third group - 3%, and the fourth group - at a rate of 5% of weight of dry matter of forage. During the experiments there were maintained optimum parameters of microclimate, recommended temperature and light regimes and a sufficient ultra-violet irradiation. As a result of the realized research it was established, that use of the mineral dolomite additive in feeding of laying hens proved to be possible as a substitute of an expensive import cockleshell. Use of various doses of dolomite promoted improvement of mineral metabolism, increasing of productivity and improvement of egg quality of laying hens.

In the conditions of the Republic of Belarus there was determined the productivity and foraging advantages of fodder galega (Galega officinalis) in comparison with the traditional perennial leguminous grasses (alfalfa (Medicago sativa); alsike clover (Trifolium hybridum); cookshead (Onobrychis viceaefolia)) and influence of the analyzed crop of state and internal microbiota of agricultural animals. There was studied the qualitative structure of galega in accordance with the phases of crop development and its influence on physiological functions of animals. It is established, that in the conditions of northern region of Belarus the fodder galega provided the yielding capacity of dry matter of 11,3 t/ha that exceeded the indices of alfalfa on 13,9%, alsike clover - in 2,7 times and cookshead - in 2,1 times. By the third decade of May it formed 18-20 tons green mass that made it possible to feed animals on 10-15 days earlier, than with other leguminous grasses. High degree of leafage (65,7%) preconditioned its high nutritional value. Fodder galega has high level of calcium, potassium, phosphorus and some microelements: copper, zinc, manganese, cobalt. Studying of vitamin value of fodder galega showed that its green mass was vitamin-rich, had enough of irreplaceable amino acids. It was established, that hay of fodder galega had high level of the non-decomposed protein (68,6-71%) that was very important for feeding of highly productive cows. It increased the level of crude protein in experimental animals on 8%.

In the conditions of the Republic of Belarus there was created an adhesive antigen on the basis of bacterial suspension of Escherichia which were separated from culture media by means of purification and concentration on membranes UMP-300 and UMP-5 that made it possible to obtain a cleaned-up preparation the specific activity of which was in 2-4 higher that of the unpurified one. In course of the study there was developed a method of purification and concentration of adhesive Escherichia antigens with the help of membrane technology. There were obtained biologically active adhesive antigens E.coli K88, K99, F41, 987P; there were selected the ultrafiltration membranes providing the optimal selectivity and productivity of the process; there were studied the influence of some factors on the process of adhesins concentration by means of ultrafiltration method. During the experiments there was analysed the agglutinating activity of antigents prepared by different methods.

In the conditions of the Republic of Belarus there was studied the application of sodium chloride obtained by electrolysis method on Akvamed installation in a complex of activities for the preventive measures of animal helminthoses. Electrochemical activation of aqueous solution of sodium chloride proved to be a progressive and rational technology which made it possible to substantially reduce the material costs and power consumption for the development of highly efficient and ecologically safe disinvasion solution. Application of sodium chloride solution with the minimal content of available chlorine of 3,0 g/l provided the death of 94-95% of Taeniidae eggs in course of 30 minutes after applicated of the preparation on the treated surface. The proposed method can be recommended as the most efficient and economically effective for the disinvasion of external environment.

In the conditions of the Republic of Belarus there was analyzed fauna of parasites of gastro-intestinal tract and natural resistance of horses in the conditions of Polesye State Radiation Ecological Reserve. There were worked the most efficient antihelminthic compounds. Analysis of helminthological state showed that extensiveness helminthic and gastrophilosis invasion of horses was at a high level. In this regard the nematodosis and gastrophilosis (Gastrophilus intestinalis) invasions prevailed. In mature and growing horses there was marked the dominance of nematode worms of Strongylidae / Trichonematidae families (78,9-83,8%) and Gasterophilidae (95,8%). Next, at growing horses there were stated nematodes Strongyloides westeri and cestoid worms of Anoplocephalidae family, at mature horses – of Gasterophilidae, Parascaris equorum and Oxyuris equi families. The following antihelminthic compounds ans preparations showed the best efficiency: Univerm; Avermektin paste 1%; Fenbendazoli, Rivertin 1%.

In the conditions of the Republic of Belarus there was analyzed the influence of inclusion into broiler chickens diets of probiotoc preparation Bifidoflorin zhidkij (liquid) and prediotic veterinary drug Biofon AIL on the natural resistance, hematopoiesis, growth, metabolic function, development and viability of broiler chickens. In course of the study there was analyzed the dynamics of total haemoglobin, total protein of blood serum, albumins and globulins of broiler chickens which were fed with veterinary drugs probiotoc Bifidoflorin zhidkij and prediotic Biofon AIL. Indexes of chicken viability and life weight gain were marked. Research results showed that application of the presented veterinary preparations rendered the growth promoting action, lowering of disease incidence, increasing of chicken viability, increasing of live weight gain and stimulation of metabolic processes in the most intense period of poultry growing. All these factors were expressed in highest degree after the combined application of the probiotic and prebiotic veterinary drugs.