Antiviral effect of crude aqueous extracts of Neem leaves and Neem bark (Azadirachta indica) belonging to the family Meliaceae againstvelogenic Newcastle Disease virus was studied. Maximum non- toxic dose and determination of antiviral activity by in vitro and in vivo virus inhibition assay was carried out using embryonated SPF chicken eggs and SPF chickens. Different concentration content of the aqueous neem extract from branches of neem tree storing at 4°C reacted against velogenic ND virus was conducted. Determination of antiviral activity by in vivo assay in SPF chickens was compared to the group of untreated with Neem extract.

The faecal egg count reduction test (FECRT) was conducted on local goats in a smallholder farm on the outskirts of Ipoh. Levamisole drug tested for resistance against strongyles, was administered orally at the rate of 10 mg/kg body weight. Results showed that thestrongyle worm population was resistant to levamisole. The percentage reduction was 75% and lower confidence limit less than 90%. The worm population was made up mainly of Haemonchus contortus(71%), followed by Oesophagostomum sp.(18%) and Trichostrongylus sp. (11%). This finding indicates that anthelmintic resistance is an existing problem in Malaysia, even on a small farm. Alternativeapproaches to chemical anthelmintics have been recommended to controlnematodes in goats such as improved grazing management, herbal medication or biological control, to delay the occurrence of chemical resistance and prevent severe helminth infections in goat flocks.

A case of a cestode parasite (Choanotaenia infundibulum) egg wasfound in the intestine of chicken which was sent to the Veterinary Research Institute, Ipoh in February 2014 for post-mortem. Other parasites found in the intestine were adult tapeworm and oocyst ofcoccidia. The examination of the chicken intestine for helminth ova was undertaken using direct examination of a simple wet smear of the intestinal content which was observed under compound microscope.The cestode parasite was identified based on helminthological keys by Soulsby, 1982.

The reports on wing bone fractures are common in avian species, butare scanty with peafowl. An Indian peahen (Pavo cristatus) was presented with a wounded unstable left wing and inability to fly. Crepitus could be felt on palpation and the radiographic examination showed an open, transverse, mid-diaphyseal fracture of humerus. Open reduction, internal fi xation and wound closure were performed under general anaesthesia followed by external coaptation of the operated wing. Post-operative antibiotics and analgesics were administered. Bythird week, clinical signs of healing were noticed and physical therapy was advised.The bird recovered uneventfully and was released. A unique case on management of transverse humerus fracture rarely reported in peafowl is documented.

Blastocystis is considered to be a zoonoses and it is believed that animals such as chicken constitute large reservoirs for human infection via the faecal-oral route. Therefore, Blastocystis infection was surveyed in free-range chicken and cage reared chicken comprising broiler birds for consumption as well as jungle fowls andsilkie chicken kept for recreation. Fresh faecal samples collected were examined by wet smear preparation and were cultured in Jones medium supplemented with 10% horse serum. Out of 107 chickens, it was found that most of the free-range chicken was positive for Blastocystis sp. with a high prevalence rate of 80%–100% in village chicken, jungle fowl and white silkie chicken. However, the cage-reared chicken, consisting of broiler chicken had no infection. The vacuolar form was the most common Blastocystis cell form found in cultures, similar to B. hominis.These cells were usually spherical and vary greatly in size, ranging from 10 μm to 30 μm in diameter. Owing to the free ranging and scavenging habits, the likelihood of acquiring the infection fromthe environment contaminated with the faecal material of animals with Blastocystis is high in free-range chicken as compared to caged chicken.

A study carried out on the presence of parasites, both internal andexternal, in 29 stray and 38 pet dogs in Ipoh, Perak indicated that four species of ectoparasites and four species of endoparasites were identified. Samples collected were diagnosed at the VeterinaryResearch Institute (VRI) whereby it was found that a higher percentage of stray dogs were infected (76%) with parasitesas compared to pet dogs (16%) that were presented at the government veterinary clinic. Parasitic infections in dogs are especially important as Demodex sp., Giardia sp., Toxocara sp. and Ancylostomasp. are zoonotic and can cause skin infections, mange, diarrhoea and anaemiain humans. Regular screening of pets is important to stave off unwanted infections. As for stray dogs, strict enforcement tocontrol stray dog population and public awareness campaigns on uncontrolled breeding of dogs needs to be emphasised.

This study was conducted for the detection of Cryptosporidium (C.) parvum infection in neonatal calves. A total of 220 fecal samples (diarrheic 110 and non-diarrheic 110) of neonatal calves were collected from Mosul city, Iraq over a period of 16 months from November 2010 to March 2012. The age of the calves ranged from 1 to 30 days. All the fecal samples were analyzed by capture direct ELISA. The infection was found in 29.0% (n=32/110) of the diarrheic calves. The infection was mostly prevalent (p<0.001) in the calves of three weeks of age. No C. parvum infection could be detected in the non-diarrheic animals.

This study aimed to determine the seroprevalence of Mycoplasma gallisepticum (MG) infection in the chicken population of Bhola district, Bangladesh, during the period from April 2011 to March 2012. A total of 480 blood samples from chickens were collected from different upazilas (sub-districts) of Bhola district. The sampling considered the types of chicken (backyard and commercial layer), age groups (pullet, adult and old) and seasons (summer and winter). On the basis of the serum plate agglutination test, 55.83% (n=268/480) chickens were found positive for MG. The MG infection was higher (62.5%) in backyard chickens as compared to those being reared in commercial farming systems (53.61%). With respect to age groups, the prevalence was highest in pullets (60.63%) followed by adults (55.63%) and old chickens (51.25%). Moreover, chickens reared in winter showed higher prevalence of MG (60.42%) as compared to those reared in summer (51.25%). In conclusion, MG infection is prevalent in the chicken population of Bhola district, Bangladesh. Appropriate strategies should be taken for successful prevention and control of this disease in Bangladesh.