The current study utilized 20 postpartum anestrus buffaloes maintained at an organized dairy farm in Vuyyuru, Krishna district to compare the metabolic (glucose), mineral (calcium) and anti-oxidant (superoxide dismutase) levels in ovsynch-48 (n=10, G48) and ovsynch-56 (n=10, G56) postpartum anestrus graded Murrah buffaloes. G48 group of buffaloes received 20 µg of GnRH on Day 0, followed by 500 µg PGF2 on Day 7, a final dose of 20 µg of GnRH was given 48 hours after PGF2α on Day 9. Whereas, G56 group of buffaloes received 20 µg of GnRH on Day 0, followed by 500 µg of PGF2α on Day 7, a final dose of 20 µg GnRH was given 56 hours after PGF2α on Day 9. In both groups, FTAI was performed at 16-21 hours interval after observing estrus. Results revealed that on different days of treatment, serum glucose and calcium levels did not differ significantly (P>0.05) in both treatment groups; however, Superoxide dismutase levels varied significantly (P<0.05) on different days of treatment in both groups. The results of the study parameters could play pivotal role in reproductive function and may provide insights into the underlying mechanism of anestrus.

The present study was aimed to assess the efficacy of oocyte retrieval through transvaginal ovum pick-up (OPU) in Ongole (Bos indicus) cows. Cows (n=18) were divided in to two equal groups; cows in group 1 cows were subjected to two OPU sessions (OPU1 and OPU2) at 96 h interval irrespective of the stage of estrus cycle. Cows of group 2 were subjected to FSH pre-stimulation before OPU 1 followed by OPU 2, 96h later. Thus, a total of 36 OPU sessions were performed on 18 animals.  The number of follicles available for aspiration (17.89 ± 1.78 vs 27.06 ± 1.75), number of medium (4 – < 8 mm; 4.11 ± 0.69 vs 16.00 ± 1.76) and large follicles (≥ 8mm; 1.06 ± 0.23 vs 6.33 ± 0.79), follicles aspirated (11.95 ± 1.42 vs 17.45 ± 2.07), COCs recovered (5.72 ± 0.78 vs 10.06 ± 1.78), and viable COCs collected (4.23 ± 0.67vs 8.34 ± 1.79) were significantly higher in group 2 than in group 1.The mean number of follicles aspirated, the mean oocyte recovery and the viable oocytes collected were significantly higher at OPU 1 in both the groups than at OPU 2. It was concluded that pre-treatment with FSH increased the OPU efficiency in terms of oocyte yield and viable oocytes collected. OPU 2 performed at shorter interval after OPU 1 is not advantageous due to limited number of follicles available for aspiration at OPU 2 and consequently, reduced oocyte recovery.

The present study was conducted to study the effect of maize (Zea mays) cob on replacing paddy straw in the Total Mixed Ration (TMR). Twelve cross bred calves of about 5 to 8 months of age with body weight ranging from 41 to 79 kg were divided into two groups of six each in completely randomized design. Five complete diets were prepared (TMR1 to TMR5) using maize cobs at the level of 0%, 25%, 50%, 75% and 100% replacement of paddy straw in the diets containing 12 % CP and 60 % TDN of 50:50 concentrate to roughage ratio. There was significant (P<0.01) differences among the diets in OM, CF, NFE, TA, AIA, NDF, ADF, Lignin, Hemicellulose and Cellulose contents. In vitro rumen fermentation study showed significantly (P<0.01) higher total gas (ml/200 mg/48 h), in vitro dry matter and organic matter degradability in maize cob based ration than paddy straw contained ration (51.17 vs 36.00; 62.87 vs 57.25; 64.80 vs 59.93). Paddy straw (100%) based diet as control ration and maize cob (100 % replacement of paddy straw) based diet as treatment ration fed to growing calves for sixty days in growth trial. A seven day digestion trial was conducted in the middle of the experiment. The digestibility (%) of DM, OM, CP, EE, CF and NFE were significantly (P<0.01) higher in the maize cob based diet fed group than paddy straw based diet fed group. The average body weight gain (kg) and FCR (kg DMI/ kg gain) were significantly (P<0.05) higher in maize cob fed animals (19.47; 6.10) than paddy straw fed animals (16.02; 7.34). The feed cost per unit of weight gain in calves fed paddy straw based diet was numerically higher (30.78 %) than calves fed on maize cob based ration. It could be concluded that maize cob based ration could replace paddy straw at 100 % level in total mixed ration without affecting feed intake and nutrient digestibility and may also improve the body weight gain and reduce feed cost in growing calves.

This case report details the episiotomy approach employed in managing vulval hypoplasia in a two year old Miniature Pomeranian bitch. The animal was presented to the Teaching Veterinary Clinical Complex, Veterinary Gynaecology and Obstetrical Unit, Madras Veterinary College, displaying a history of greenish black discharge and continuous straining for two hours. The animal was bred before 64 days. Physical examination revealed a small vaginal opening, measuring approximately 1.5 cm in length. Digital examination indicated fetal hind limbs and tail extending into the vaginal passage, while all other vital parameters were within the normal range. Attempts to induce uterine contractions using Inj. Oxytocin and 10 % Calcium gluconate in 5 % DNS proved unsuccessful. Therefore, under local anesthesia (2 % Lignocaine injection), median episiotomy was made and a single live male puppy was successfully delivered with mild traction. Closure of the incision site was accomplished using PGA 1/0 with a simple interrupted suture pattern. The patient had uneventful recovery after 5 day treatment regimen, involving a single dose of subcutaneous Inj. Cefovecin (antibiotic) and anti-inflammatory drugs, along with meticulous wound management.

A case of fetal mummification among one of the quadruplet kids was reported in a fourth parity Kanni adu doe.

The present case report explains management of cervico-vaginal prolapse in a eight months pregnant Holstein Friesian crossbred cow. The proper therapeutic and management practices were applied for easy reposition and correction of cervico-vaginal prolapse. The results conclude that cervico-vaginal prolapse occurs due to one or more etiological factors including impaired endocrine function due to feeding with mustard fodder.

The use of metallic silver as an antimicrobial agent has been recognized for centuries. Silver nanoparticles (SNP) are now incorporated in apparel, wound dressings, appliances, cosmetics, paints and plastics for their antimicrobial properties. Generally, silver nanoparticles are prepared by a variety of chemical methods. In this study, we have used Phyllanthus amarus and Andrographis paniculata herbal plants leaves and stem extracts to synthesis SNP and were characterized by UV-Vis spectroscopy and Transmission Electron Microscopy (TEM). SNP capped with plant extracts gave absorption peak at 420 nm as expected for silver and broadening of peak were also noticed. TEM images suggested that they were of almost spherical shape and in the range of 7-60 nm in size. Antibacterial activity of plant extracts capped SNP were tested in Mueller-Hinton agar by well diffusion method against Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, Escherichia coli and Salmonella Typhimurium.  SNP of 1 mg/ml and 5 mg/ml of P.amarus exhibited almost similar antibacterial activity, whereas, 5mg/ml, not 1 mg/ml of SNP capped with A. paniculata exerted antibacterial activity. The highest zone of inhibition was noticed against B.subtilis followed by S.aureus and P.vulgaris and the least zone of inhibition was observed against E.coli and S.Typhimurium. Green synthesis of SNP is cost effective and environment friendly. Further studies are required to explore the possibility of use of P.amarus capped silver nanoparticles for the treatment of burn and wound.

The present study was carried out to find out the histopathological alterations in gill, liver and kidney of Goldfish (Carassius auratus) under sublethal concentrations of Zinc. Fishes were exposed to sub-lethal concentrations of 0.572, 1.145, 2.289, 4.578 and 9.156 mg.l -1 for 28 days which are 1/10 of their 96hr LC. The histological samples of gill, liver and kidney were collected from 14 th, 50th  and 28th day and stained by haematoxylin and eosin, observed under trinocular microscope. Vacuolation, hyperplasia, epithelial lifting and lamellar fusion, shrinkage of blood vessels, secondary lamellar damage, breakdown of epithelial cells, blood congestion and necrosis were observed in gill. Rupture of nucleus, congested blood vessels, hemorrhage in liver sinuses, increased size of kupffer cell, increased pycnotic nucleus, hypertrophied hepatocytes, vacuolation, cellular necrosis, rupture of hepatocytes were observed in liver of zinc treated fish. Shrunken glomerulus, vacuolation, blood congestion, increased in space of periglomerular, increased in space of peritubular, melanomacrophages, loss of cytoplasm, fat deposition, degenerated tubules and degenerated glomerulus were seen in kidney. The above results showed that sub-lethal toxicity of Zinc alter the internal organs of goldfish at lower concentration.

Restraining gadget for Indian poisonous snakes like Indian cobra and Russell’s Viper were designed, using available infrastructures. Tongs, collapsible tubes and bags were designed and applied in the poisonous snakes in order to have better restraining activities. These snakes reared at Guindy Snake Park Trust were successfully restrained, using the gadgets developed. However these gadgets were found not suitable for restraining procedure associated with Saw scaled viper and Common krait. The gadgets developed were useful for zoo veterinarians for control of these serpentines.

A study was undertaken to evaluate carbohydrate and protein fractions of crop residue based complete rations as per the procedure of Cornel Net Carbohydrate and Protein (CNCPs) system.  Three iso-nitrogenous complete rations were prepared comprising of maize stover (T1) straw , red gram straw (T2) and black gram (T3) and concentrate mixture in 60: 40 ratio, respectively and were analyzed for CNCPS fractions. Maize stover contained lower (P<0.01) lignin (% NDF) compared to red gram or black gram straws. Lignin (% NDF) was lower (P<0.01) and starch (% NSC) was higher (P<0.01) in T1  when compared to T2 or T3. NDICP (% CP) was higher (P<0.01) and ADICP (% CP) was lower (P<0.01) in T1 when compared to other complete rations. Carbohydrate fraction CA (% CHO) was higher (P<0.01), while fraction CC (% CHO) was lower (P<0.01) in T1 when compared to other complete rations. Protein fractions PB1 and PB3 (% CP) were higher (P<0.01) in T1 while the fraction PC (% CP) was similar among the complete rations under study. Similar trend of higher (P<0.01) CA, PB2 and PB3 and lower (P<0.01) CC fractions were observed in maize stover than in red gram or black gram straw. The present study concluded that complete ration (T) comprising of maize stover was better in terms of carbohydrate and protein fractions with higher (CA, PB1and PB3) and lower CC and PC fractions when compared to other complete rations.