This experiment was conducted to compare six serological tests for diagnosing bovine brucellosis standard plate agglutination test (SPT), standard tube agglutination test (STT), complement fixation test (CFT), Rivanol test (RT), agar gel precipitation test (AGP) and counter immunoelectrophoresis (CIEP) in using 38 sera from brucella reactors and 222 sera from cattle. The SPT gave 1.6% apparent false negative reactions and 15.4% apparent false positive reactions when compared with STT which is an official test for bovine brucellosis in this country. The distribution of antibody titers determined by STT showed that 37.5% of 38 reactors had antibody titers of 400 or higher. When 38 reactors judged by STT were tested by CFT and RT, 32 cattle (82.4%) were positive by CFT and 33 catlle (86.8%) were positive by RT respectivily. This results suggest that Rt is comparable to CFT in the diagnosis of bovine brucellosis. The results also indicated that both AGP and CIEP were insensitive to detect brucella infection in cattle

A seroepidemiological survey for antibodies to bovine leukemia virus in dairy and Korean native cattle in Kyungbuk area, Korea [R.] was performed using BLV p and gp antigens by the ID test. In the dairy cattle (Holstein), thirty of 106 sera (28.3%) form 14 dairy farms were positive for BLV antibodies and 12 of 14 pastures were contaminated with BLV. In the Korean native cattle, seventeen (2.4%) of 699 sera from the slaughter house in Taegu city were positive for BLV antibodies. Of 47 gp-ID positive cattle, 5(10.6%) had antibodies to p antigen

A polyvalent live Eschericia coli oral vaccine were prepared by the use of 5 field isolates of enteropathogenic E. Coli, serotypes 08: K87, K83a, b; 0138: K81, K88a, c; 0141: K85a, b, K88a, b: 0149: K91, K88a, c and 0157: K'V17' K88a, c. Some field experiments were conducted to investigate the effectiveness of the polyvalent live E. coli oral vaccine in the prevention of neandatal colibacillosis in piglets suckling orally vaccinated sows. Seventy-nine pregnant sows in and intensive pig farm were vaccinated with oral vaccine 4 weeks prior to farrowing and 19 sows were chosen for control. The piglets suckling vaccinated sows showed a significant protection against neonatal enteric colibacillosis during the 2 weeks observation period from the farrowing but no significant differences in protection between vaccinated and control group were observed with piglets older than 15 days

Pathological studies on 6 milk goats administered with urea were carried out to determine the effects of acute and chronic poisoning on the animal. Clinical signs appeared about 10 minutes after ingestion of the urea and they consisted chiefly in dullness, staggering, uncontrolled urination, dyspnoea, frothy salivation, bloating, muscular tremor, humping of back and tetanic spasms. Macroscopic lesions were failure of blood clot, frothy and bloody contents in the highly congested respiratory tract, pulmonary edema, rumenitis and abomatitis accompanying with petechiation of the upper small intestine. Microscopically, the liver showed vacuolar degeneration, fatty change and areas of necrosis and partial proliferation of the interlobular connective tissues in chronic cases. The renal epithelium displayed vacuolar degeneration, necrosis and hyaline casts in their lumens. In the lungs, there were hyperemia, edema, hemorrhages, and acute catarrhal bronchitis. Neuronal degeneration and necrosis in the central nervous system, cattarhal rumenitis, abomatitis, doudenitis and myocaridal hemorrhage were also demonstrated

This is a study of bovine viral leukosis in a young cattle (10 months old). Main clinical symptoms observed were emaciation, digestive disorder, and marked enlargement of superficial lymph nodes. Biopsied specimen of the lymph nodes revealed a characteristics histological picture of bovine leukosis. A severe degree of leukocytosis (108,000/cmm) accompanied by marked lympocytosis (96%=680/cmm) was detected from the hematological examination of peripheral blood. Most of the lymphocytes (94%=101,520) were large-sized, immature, and abnormal in their morphology. In addition, a pronounced anemia (1.78 million/cmm) without regenerative evidence of erythrocyte was noted. The serum of the patient showed positive reaction against gp antigen of bovine leukosis virus in agar gel diffusion test

This paper dealt with histopathological observations of the nephroblastoma in the pregnant pig which died 8 days before parturition day. In the necropsy findings the pregnant pig died of circulative and respiratory disturbance by compression of fetus and renal tumor weighing about 12 kg. In the microscopical findings of the renal tumor it was composed of well differentiated tumor cells and connective tissue elements. As differentiation of the neoplastic epithelial cells progressed, tubular and cystic structures, and papillary structures resembling glomeruli appeared. This tumor was regarded as nephroblastoma

This study was done to clarify the histopathological changes of pigs naturally infected with hog cholera. Microscopic observations of the necrotic lesion and inclusion body in the lymphoid organs were carried out in the natural cases of hog cholera and experimental cases inoculated with ALD (challenge) virus and isolated virus strains. Electron microscopic findings of the intranuclear inclusion bodies in the retincular cell of spleen and lymph node were also observed in the experimental cases. The histological findings of necrosis of lymphoid organs were found to contain 35.0% in the natural and 37.5% in the experimental cases. Intranuclear inclusion bodies were found mainly in the reticular cells of lymhoid organ, the epithelium of bronchiole and alevolus, and the vascular endothelium of brain. These inclusion bodies were seen in 40.0% of the natural cases and in all of the experiment cases. The inclusion body appeared to be composed of activated nucleoli and chromatin granuls (interchromatin and perichromatin) by electron microscopy

A seroepidemiological survey on bovine leucosis virus was conducted. Serological studies were analyzed by age groups and the sizes of herds. The number of reactors increased gradually with the advance in the age of cattle and the herd size. The highest rate of BIV carriers was found in the ages between 6 and 8 years, and in the size of herds with 20 to 50 heads. Two out of 6 specimens (33.3%) from the reactors showed the typical type C virus with the size of 90 to 110 nm around microvilli and in intracytoplasmic vacuoles

This experiment was carried out to know the 133 isolates of yeast-like fungi. The antifungal agents tested were nystatin, griseofulvin, cycloheximide, 5-fluorocytosine, miconazol, clorimazole and tolnaftate. In general, clotrimazole, miconazol and 5-fluorocytosine were more effective in antifungal activity in vitro against the test organisms than the rest of the agents tested. However, some of the isolates showed higher resistance to certain antifungal agents compared to the other isolated of the same species. They were: 1C. albicans isolate to 5-fluorocytosine: 1 C. albicans to 5-fluorocytosine, miconazol and clotrimazole: 1 c. krusei to 5-fluorocytosine and cycloheximide; and 11 C. tropicalis isolates to cycloheximide. The minimum inhibitory concentrations (MIC) of clotrimazole were 12.5 mg/ml or lower for all isolates tested except one C. albicans isolate, for which MIC of the drug was 100 g/ml. On the other hand, the MIC's of cycloheximide were 6.5 mg/ml or lower for all isolates except the following: all isolates of C. albicans (100 mg/ml), 11 C. pseudotropicalis (200 mg/ml) and Rhodotorula sp. (25-50 mg/ml), 11 C. tropicalis isolates (100 mg/ml) and 1 C. Krusei isolate (200 mg/ml)