As a fundamental study to increase the reproductive efficiency in cattle, highly motile sperm were separated and collected from raw semen, extended semen and frozen semen by different methods using various concentrations of bovine serum albumin or Tyrode's solution. Various characteristics and light microscopic and electron microscopic morphology of sperm separated by different methods were compared

The in vitro activity of penicillins (penicillin G, ampicillin, carbenicillin, methicillin and cloxacillin) and gentamicin to Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitic milk samples was determined. The growth of all isolates of staphylococci tested was inhibited by cloxacillin and methicillin at concentration of 0.78 micro g/ml and by gentamicin at a minimum inhibitory concentration (MIC) of 1.56 micro g/ml

In this study lactating female rabbits and strains of coliforms previously isolated from acute and chronic mastitis cases in dairy cattle were used. The pathological changes on mastitis experimentally induced with the coliform strains and the mammary glands after infusions of Escherichia coli suspension together with dexamethasone, dextran iron or transferrin were grossly and microscopically observed. In the bacterial suspension-infused groups by E. coli, Klebsiella pneumoniae and Enterobacter aerogenes, the affected quarters of udder showed gross swelling, hyperemia, hemorrhage, focal necrosis and firmness

The study was to find out the changes of hormone levels in blood serum and milk of Holstein cows during the estrous cycle. The progesterone, estradio 1-17beta from the blood serum and milk samples were assayed by radioimmunoassay methods. During 20 to 25 days after artificial insemination, the accuracy of pregnancy diagnosis from the blood serum progesterone levels were 87.5% for non pregnant cows (2.Ong/ml) and 83.3% for pregnant cows (3.Ong/ml). The accuracy of pregnancy diagnosis from the milk progesterone levels were 75.0% for non-pregnant cows (2.4ng/ml), and 94.4% for pregnant cows (3.2ng/ml)

A migratory bird, the white stork Ciconia boyciana was captured from a pond in the Chejudo, Korea R. on the 18th of Dec. 82. The morphological characteristics were described as seen in the binocular stereoscopic microscope and further fine structures were observed by means of the scanning electron microscope (model; ISI-DS-130). The bird was examined for ectoparasites at the Cheju Provincial Veterinary Research Lab. Two kinds of specimens were collected from the head and body feathers. The specimens were sent to the Inst. of Veterinary Research in Anyang, Korea R. for classification and identification

Scanning electron microscope (model; SEM ISI-DS-130) was used to observe the fine surface structures of the predominant cattle tick Haemaphysalis longicornis Neumann 1901 (Acarina; Ixodidae) in Korea R. and some SEM microphotographs were presented in this pictorial monograph. A total of 18 photographs was selected from the numerous SEM photographs of the female and male specimens of H. longicornis for the observations of the fine surface structure and the comparison between the female and male structures

Cells with an epithelioid morphology were isolated from the rabbit myometrium and were grown in culture. The cells had a doubling time of 53 hrs when grown in the presence of 10% fetal calf serum in Basal Eagle's medium with 3mM glutamine. In the presence of estrogen plus insulin, doubling time was reduced to 40 hrs. Creatine kinase activity upon reaching confluency was determined to be 0.019 uit per mg protein. Approximately 30% of the activity was extractable only in high ionic strength buffer. Cells also contained plasminogen activator with a specific activity of 140 CTA units per million cells

The research was conducted to confirm the agent responsible for the antimicrobial activity contained in the fermented tomato juice with Lactobacillus acidophilus to extract and purify the antimicrobial agent to find the biological, physical and chemical properties of the agent. The agent responsible for the inhibitory activity was confirmed by both well assay method using fermented tomato juice with L. acidophilus and turbidimetric technique using the cell-free filtrate or neutralized filtrate of tomato acidophilus culture and found exerted antimicrobial agent other than lactic acid

To investigate the factors influencing the artificial transformation in Escherichia coli, E. coli C 600 was transformed by pBR322 DNA with tetracycline and ampicillin resistant gene purified by CsCL-EtBr equilibrium density gradient centrifugation from E. coli HB 101. The best conditions for the transformation of E. coli C 600 with pBR322 DNA were treatment with 100mM CaCl2, ice incubation at 0 deg C for 45 minutes, heat shock at 42 deg C for 4 min., 30 min. of ice incubation and incubation at 37 deg C for 100 min. for gene expression in that order

Gross and histological findings were obtained on cysts formed in the reproductive organs, particularly in the mesosalpinx, mesovarium, and fimbria, of 144 gilts and 37 sows. Of the 181 gilts and sows, 63 (34.8%) were found to have cysts which totaled 89. The number of cysts totaled 38 for the gilts and 51 for the sows, with relative frequencies of 18.1% 48.6%, respectively. The total number of cysts and the incidence were greater in the sows than in the gilts