The study was carried out on male zebrafish containing technical grade deltamethrin (98.5%). A total of 20 males were randomly divided into two groups (Group I) and Group II (Group II) and their pre-exposure spawn count, fecundity, and embryo mortality were calculated at weekly intervals within each group. Results show significant (p0.05 and 0.01) reduction in total count of embryos over 21 days in Group I. Similarly, in Group II it was 36.49 0.54, 72.740.09 and 86.370.06 at 7, 14 and 21 days respectively postexposure of males to deltamethionine.

Biometry and follicular parameters of porcine ovaries sourced from organized and unorganized-slaughterhouses and relative comparison of oocyte retrieval methods, namely aspiration and slicing, are reported in the present study. Average length and width of the ovaries, and numbers of surface and cystic follicles were higher in ovaries sourced from organized slaughterhouses. Average number of oocytes recovered per ovary by slicing (12.93±1.49) was significantly higher (P<0.01) than aspiration (6.36±1.02). However, aspiration yielded higher percentage (65.21±5.71) of good quality oocytes (A) (P<0.01) suitable for upstream applications of assisted reproductive technologies (ART). Further, our study reveals that ovaries sourced from unorganized slaughterhouses can also be used for ART, and use of aspiration method may yield better quality oocytes.

The plant Limonia acidissima belongs to family Rutaceae. Various parts of this plant like fruit, seed, rind, bark, leaves and roots are being used to treat human and animal ailments. The fruits of the plant are most essential therapeutic portion containing bioactive molecules. This study is intended to reveal presence of some phytoconstituents from leaf extracts and review it thoroughly in order to validate its medicinal use. In the study it was found that aqueous and ethanolic extracts revealed maximum types of phytochemical classes as compared to acetone and petroleum ether extract which can be used for treatment with reference to its mentioned use. Many phytochemicals namely alkaloids, carbohydrates, glycosides, proteins, saponins, phenols, resins, etc. were detected which  reflects its therapeutic value.

Contamination of locally fermented cow milk (Nono) by pathogenic bacteria constitute a major public health problem. This was because contaminated milk was a good medium that can support the growth and propagation of microbial pathogens. This study was designed to assess the bacteriological quality of locally fermented cow milk consumed in Maiduguri, northeastern Nigeria. Totally, forty (n = 40) samples of fresh, locally fermented cow milk (Nono) were collected and used in this study. Ten (10) milk samples each were collected from four randomly selected selling points (Monday Market, Custom Market, Kasuwan Shanu and Tashan Bama). The bacteriological quality of each milk sample were analysed using standard procedures for isolation, identification and enumeration of pathogenic bacteria. The mean total plate count ranged from 1.44x10 8 CFU/mL to 10.31x10 8 CFU/mL, while the mean total coliform count range from 0.57x10 8 CFU/mL to 11.17x10 8 CFU/mL. Statistically, there was a significant difference (p<0.05) in the mean total plate count and coliform count of milk samples collected from the four sampling points.Bacteriological culture and biochemical identification of all the isolates revealed Escherichia coli29(72.5%), Klebsiella specie3(7.5%), Salmonella specie 2(5%), Staphylococcus aureus 14(35%) and Streptococcus specie15(37.5%) respectively. All the results were above the acceptable limits (1.0 to 3.0 x 10 5 CFU/mL for total plate count and no coliform count per 100ml of milk sample) set by NAFDAC. Therefore the Nono products consumed in Maiduguri was not safe and wholesome for public consumption. Hence, the need to ensure the maintenance of standard hygienic protocols during the collection, storage, processing and marketing of locally fermented cow milk (Nono) in order to minimize microbial contamination.

Milk is one of the primary protein diets of high biological value available to the human population. As bovine beta casein gene is highly polymorphic with functional implications, this study was designed to detect the beneficial A2 genotype in the native Umbalachery cattle breed. Genomic DNA was isolated from blood samples of Umbalachery cows and A1 and A2 variants were detected by Tetra ARMS polymerase chain reaction. It was observed that the A2A2 genotype frequency was 82% and that of A1A2 was 18%. The A1A1 genotype was not detected in Umbalachery cattle in the present study. It may be concluded that majority of the Umbalachery cows retain their original beta casein protein variant that produce safer A2 milk for human consumption and that Tetra ARMS PCR is an efficient and rapid method that allows detection of single nucleotide polymorphism of A1 and A2 variants of beta casein gene.

Physico-chemical parameters of fresh milt and mitochondrial activity of fresh spermatozoa of the endangered golden mahseer, Tor putitora (Hamilton) were investigated. Cryopreservation and fertility evaluation of cryopreserved-thawed spermatozoa of T. putitora were carried out. The concentration of spermatozoa and the spermatocrit values of the fresh milt were estimated to be 3.96±0.12 x 107 spermatozoa/ml and 70.00±1.53% respectively. The motility percentage of spermatozoa in the fresh milt was estimated to be 94.84±0.43. The elemental composition analysis revealed that the concentration of K+ was higher i.e., 14.13±0.32 mg/l when compared to that of Na+, Ca+, Mg+ and Zn+. The concentration of total reducing sugars and total proteins were estimated to be 51.79±0.47mg/100 ml and 40.57±0.75 mg/100ml respectively. The absorbance value of the fresh spermatozoa in Sperm Mitochnodrial Activity Index (SMAI) assessment by Nitro Blue Tetrazolium (NBT) assay was estimated to be 0.33±0.012. The optimum sperm: egg ratio was estimated to be 3.96 ±0.12 x 104+ no./egg. Hatching percentage of 45.97±1.72 was recorded for the cryopreserved-thawed  spermatozoa while the fresh milt control recorded a hatching percentage of 73.10±0.82.

The present study was undertaken to induce gonadal maturation of Mrigal, Cirrhinus mrigala using Human Chorionic Gonadotropin (HCG) implants in captive conditions. The male and female fishes of C. mrigala were implanted intramuscularly with hCG implants at the dosage of 1000 IU/kg body weight, to study the effect of hCG hormone. Hormone implants were given once in two months during the experimental period of February to June. Histological observation in the ovary of C. mrigala revealed the presence of four types of growing oocytes namely perinucleolar, previtellogenic, vitellogenic and hydrated oocytes. Histological observation of the testis of C. mrigala showed the presence of five types of spermatogenic cell types namely spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa. Hence, the present study recommends that hCG implants can successfully be used to induce sustained maturation of C. mrigala in captivity even during off season. 

The prime objective of the study was to analyze the ecological features and to identify the fish species present along the upstream region of Pechipparai reservoir. Seventeen species of freshwater fishes belonging to 11 genera and 8 families have been recorded. Cyprinidae was the dominant family (6 species) in the study area. Of the 17 species, Dawkinsia arulius belong to the endangered category, Hyporhamphus xanthopterus and Dawkinsia rohani to vulnerable and Ompok bimaculatus to near threatened category. All the 17 fish species are found to be endemic to India and ornamentally appreciated.

Milk marketing agencies (Tamil Nadu Cooperative Milk Marketing (TNCMM) co-operatives and private agency) and Department of Animal Husbandry (DAH) of the Government of Tamil Nadu were providing different services viz., breeding, therapeutic, preventive, input and extension services related to dairy farming. Availability and accessibility of the above services to the dairy farmers need to be judged from the perspective of the dairy farmers, so as to streamline the services in a more efficient manner. This study was conducted following exploratory research design to understand the perceptions of the milk producers on the availability and accessibility of these services in Salem district. Multistage random sampling was adopted for selection of 150 respondents (75 milk pourers of TNCMM co-operatives and 75 milk pourers of private agencies) from the blocks of Salem district. Randomly selected respondents were interviewed personally for collection of primary data. Majority of the respondents (80 %) of both the groups perceived that AI services were accessible to them both at institution as well as at doorstep. The study also reveals that none of the agencies were able to deliver the dairy husbandry services to the farmers in a comprehensive manner. The dairy farmers preferred to have services delivered at their doorsteps for the obvious reason of the difficulty of taking the animal to the established centres. 

A study was carried out to check the prevalence of Salmonella spp. in ice cream marketed in Thrissur city, Kerala. Altogether 80 ice cream samples belonging to 10 different brands were randomly collected from different retail outlets of the city and were analysed for total viable count, coliform count and for the presence of Salmonella spp. The mean total viable count and coliform count in ice cream samples were 11.90±0.06 and 3.72±0.17 log cfu/g respectively. Thirty per cent of the ice cream samples exceeded the standards prescribed for coliform count by Food Safety and Standards Act (2006). Salmonella spp. was isolated from one of the ice cream samples. The presence of potential pathogen such as Salmonella in ice cream samples reveals the importance of strengthening the quality control measures to be followed during manufacture as well as storage and distribution of ice cream in order to reduce public health risk.