Bovine brucellosis is an enduring and formidable challenge in Bangladesh. In this first comprehensive review, we explored the historical, current, and future perspectives of bovine brucellosis outbreaks in Bangladesh. Data spanning from 1984 to 2023 regarding bovine brucellosis in Bangladesh were gathered from literature, reviews, conference papers, and online reports using various search engines and software tools. We considered 29 published documents and analyzed them thoroughly to evaluate the current status of bovine brucellosis for the present comprehensive review. We also suggest policy and other frameworks to mitigate and control the disease on a national scale. Bovine brucellosis in Bangladesh affects various livestock and poses economic burdens due to reduced milk and meat production with potential risks to human health. Over the past decade (2013-2023), the ruminant population in Bangladesh has increased substantially (between 1.46 and 26.95 million individuals), with goats showing the most significant growth, while financial losses due to bovine brucellosis have risen steadily, emphasizing its economic impact (basis on species between Euro currency 12.824 and 298.272). This review highlights brucellosis prevalence, diagnostic challenges, and traditional management practices contributing to its spread. Our findings indicate that bovine brucellosis was reported and prevalent in mild to severe forms across 26 districts of Bangladesh. Bangladesh has initiated measures such as vaccination and awareness campaigns, but effective control remains challenging due to diverse farming systems and resource constraints. A one-health approach is advocated for future strategies, emphasizing community engagement and multidisciplinary efforts to address the complex challenges posed by bovine brucellosis in Bangladesh, ultimately aiming to safeguard livestock health, public health, and economic stability. [J Adv Vet Anim Res 2024; 11(4.000): 888-913]

Objectives: This study aimed to analyze the effects of the use of binders on the physical quality and digestibility of Alabio ducks (Anas platyrinchos Borneo). Materials and Methods: Pellet binders used tapioca meal (TM) (Manihot utilissima), sago meal (SM) (Metroxylon sagu Rottb.), and sweet potato meal (SPM) (Ipomoea batatas) pelleted feed. Laying Alabio ducks, around 120 birds, aged 20 weeks with an average body weight of 1,426 ± 113.5 gm, were used. A fully randomized design with 4 treatments and 15 repeats was used in this study. The variables measured include the physical quality and digestibility of pellet feed. Data analysis used a Fisher test. For the distinction between treatments, the Duncan multiple-range test was conducted. Results: The finding showed that the plant-based pellet binder had a natural effect on physical properties, including pellet durability index, moisture content, threshold power, stack density, and stack compacted density. The strength of the pellet binder is seen in the durability index of TM 98.12%, SM 97.64%, and SPM 97.35%, respectively. However, these variables did not differ significantly in terms of specific gravity and stack angle. Pellet binders considerably affect the consumption of feed and vary markedly in dry matter, organic matter, and metabolizable energy digestibility. Conclusion: Plant-based pellet binders influence the physical quality and digestibility of pelleted feed in Alabio ducks. TM can maintain physical quality and digestibility compared to SM and SPM as plant-based pellet binders. [J Adv Vet Anim Res 2024; 11(1.000): 93-99]

Objective: In the context of the fast-expanding shrimp farming industry in Sri Lanka, this study aimed to evaluate the distribution and understanding of BMPs, examine the relationship between BMPs and disease outbreaks, and analyze the current antibiotic usage trends through a fact-finding survey. Materials and Methods: A questionnaire survey was conducted in 131 shrimp farms located in Puttalam District in North Western Province, where shrimp farming is thriving in Sri Lanka. The survey was conducted from September to October 2021. In addition, in August 2022 and August 2023, interviews were conducted with shrimp farms in Puttalam district and Batticaloa district, shrimp hatcheries, and export companies. Data were analyzed using descriptive statistics, Probit, and Tobit regression analysis. Results: The item-count technique revealed a significant (p < 0.05) difference in the use of antibiotics without consulting experts, signifying inappropriate use. No aquaculture farmer reported being unaware of BMPs, and among the 45 farmers who attended a BMPs seminar, 30 claimed to possess a good understanding of BMPs. Probit and Tobit regression results revealed that the rate of understanding of BMPs, education level, and obtaining information on the sanitation management of shrimp farming from extension and guidance organizations were inversely associated with both disease incidence in shrimp farming and shrimp discards. Conclusion: Although the use of antibiotics is prohibited in BMPs, the analysis results suggest inappropriate use of antibiotics. The findings indicate that enhanced BMP understanding can reduce disease incidence and shrimp discards, emphasizing the need for incentives to promote BMP adoption and reduce the necessity for antibiotics. [J Adv Vet Anim Res 2024; 11(1.000): 33-39]

Objectives: Keeping in view the economic and veterinary public health importance of brucellosis, this research was conducted to determine its seroprevalence and associated risk determinants in small ruminants in district Khanewal, Southern Punjab, Pakistan. Materials and Methods: Two-stage cluster sampling technique was used for sampling, and the sample size was calculated using C-survey 2.0. Accordingly, sera samples (n = 392) were collected from small ruminants in the study area from October 2022 to July 2023. All the samples were tested for the presence of anti-Brucella antibodies by Rose Bengal Plate Test (RBPT), followed by confirmation of all the samples using an enzyme linked immunosorbent assay (ELISA) kit (ID.vet®, France; sensitivity and specificity=100%, each). Results: The seropositivity rate of brucellosis was 7.14% [n = 28/392; 95% confidence interval (CI) = 4.87%–10.12%] by RBPT, whereas the results of ELISA showed an overall seroprevalence rate of 7.40% (n = 29/392; 95% CI = 5.11%–10.37%) in the study population. Univariate analysis of risk factors revealed that abortion history (AH), retained fetal membranes (RFMs), repeat breeding, flock size (FS), educational status of farmers (ESFs), awareness about brucellosis (AB), and farm hygiene had a significant association with the seroprevalence of brucellosis (p < 0.05). The multivariate analysis using a binary logistic regression model revealed that variables including tehsil, FS, AH, RFM, ESF, AB, and farming system were significant factors (p < 0.05) associated with brucellosis in the target population. Conclusion: Brucellosis is prevalent in small ruminants in Khanewal, Pakistan. The disease burden can be reduced by improving the reproductive health of animals, farm hygiene, and farmers' awareness about the diseases. Further studies are needed on a larger scale to devise stringent disease control strategies to avoid losses associated with brucellosis at regional, national, and global levels. [J Adv Vet Anim Res 2024; 11(1.000): 9-18]

Objective: The study was primarily conducted to assess the stakeholders' knowledge regarding the contamination caused by heavy metals in poultry feedstuffs. The concentration of some heavy metals (lead, chromium, cadmium, and nickel) and macro-minerals (sodium, potassium, and calcium) was also analyzed in poultry feeds collected from selected local markets in Sherpur district, Bangladesh. Materials and Methods: A well-structured questionnaire survey was used to investigate different stakeholders' perspectives in relation to metal contamination in feed. Heavy metals and calcium were determined by atomic absorption spectrophotometry. The flame emission spectrophotometric technique was applied to determine sodium and potassium. Results: The majority of the stakeholders (90%) were found to have no knowledge regarding heavy metal contamination. Lead and nickel concentrations were below the detectable level in the collected samples. The average concentration of chromium in Jhenaigati upazila was four times higher than in Nalitabari upazila, at 21.806 mg kg−1 and 5.452 mg kg−1, respectively. The concentrations of cadmium in both brand and nonbrand samples exceeded the maximum allowable limit set by the European Union at 1.329 mg kg−1 and 1.328 mg kg−1, respectively. Sodium, potassium, and calcium were found in the ranges of 0.0011%–0.0035%, 0.0010%–0.0013%, and 0.0080%–0.0305%, which were extremely low in concentration compared to the minimum requirement in poultry feed. Conclusion: Regular surveillance and governance systems should be incorporated into national policy to cease the hazardous impacts of heavy metals through feed contamination. From a nutritional viewpoint, poultry feeds need to be critically formulated. [J Adv Vet Anim Res 2024; 11(1.000): 47-54]

Objective: The study was designed to show the effect of adding different levels of dried fruit extracts for 14 days on sensory and chemical parameters in dairy drinks. The survival of Pseudomonas aeruginosa and Bacillus cereus in artificially contaminated dairy drinks fortified with these extracts was also studied. Materials and Methods: The freshly watery extracts and nonaqueous extracts of dried fruits were prepared by rotary evaporators and solvents, respectively. The determination of the minimum inhibitory concentration of dried fruit extracts was achieved using the disc diffusion test. The sensory evaluation of samples was done, while the chemical parameters of the examined samples were determined by the calibrated analyzer. In addition, the degree of survival of P. aeruginosa and B. cereus in inoculated milk samples was also estimated. Results: In pasteurized and Rayeb milk samples, the water extract of carob and all alcoholic dried fruit extracts had a significant effect on compositional parameters in comparison to control samples. At day 14 of pasteurized milk storage, the watery (20.0%) and alcoholic (10.0%) extracts of carob significantly improved its sensory parameters. Conclusion: Based on the survival results, all utilized dried fruit extracts had a significant inhibitory effect on P. aeruginosa and B. cereus growth in the fortified milk samples at the end of storage. This trial of the survival of these new dairy drinks is the first investigation, particularly in the Middle East. Extracts of utilized dried fruits have prospective functions that enhance dairy drink characteristics. [J Adv Vet Anim Res 2024; 11(1.000): 55-64]

Brucellosis is a serious disease that affects both animals and humans. It is caused by consuming unpasteurized dairy products that are contaminated with the Brucella bacteria. To study the pathobiology of this disease and develop preventive strategies, researchers rely on in vivo and in vitro models. A systematic literature search was conducted in January 2024, which revealed 38 studies that used these models in the previous four years. Mice were the most commonly used model for studying the disease's virulence genes, immune responses, vaccination, and treatment testing. Out of the 38 articles discussing infection models in brucellae, 6 used only in vivo models, 9 used only in vitro models, and 24 used both models. In addition, there were 32 studies with in vitro experiments, most of which utilized macrophages to study intracellular survival mechanisms and host-pathogen interactions. The studies mainly focused on B. abortus, as it had a significant impact on public and livestock health. Both in vivo and in vitro models were used to understand comprehensive intracellular mechanisms, immune responses, and treatment evaluations. However, there were several challenges in using these models, such as ethical concerns and host pathogen-specific immune responses. While both models provided important insights, the final selection choice of the model mostly depended on the research objectives, pathogen type, and availability of resources. Nevertheless, validation and understanding of these models are important to predict responses in the natural hosts

A laboratory-based surveillance was conducted to study the transmission of Salmonella infection in Ukraine during the period 2012-2023. The study focused on the different categories of food products, feed, and animals as the main transmission factors and tried to analyze the relationship between them. The serological profile of Salmonella was predominantly observed in samples from objects of veterinary control, including biological/pathological material from animals and biomaterials from poultry within the National Poultry Salmonellosis Control Program. The study found that the most frequently isolated serovars were S. Enteritidis (20.03%), followed by S. Typhimurium (14.76%), S. Pullorum (without biovar identification;10.71%), S. Pullorum biovar Pullorum (10.50%), S. Pullorum var. Gallinarum (6.62%), S. Choleraesuis (5.79%), S. Livingstone (2.53%), and S. Infantis (1.70%). In 2021, an isolate of monophasic S. Typhimurium was identified for the first time in pathological material from pigs. The study also found that the most frequent Salmonella-positive categories of food products in Ukraine were meat and meat products (78.16%), eggs and egg products (11.75%); dairy products (3.319%), fish products (2.71%), ready-to-eat food products (1.96%). The largest specific share of Salmonella isolates from food products and feed was S. Enteritidis, followed by serotypes such as S. Infantis, S. Typhimurium, S. Livingstone, S. Virchow, and rare serotypes such as S. Nigeria and S. Thompson. The dominance of certain serovars such as S. Enteritidis, S. Typhimurium, S. Infantis, S. Livingstone, and S. Virchow in biomaterials from sick animals indicates their primary role in the infection of food products of animal origin. Hence, a stress to enhance diagnostic and monitoring frameworks at animal herd levels. The findings of this study can be used as a basis for evidence-based epidemiology, as well as for the implementation of joint steps to improve the effectiveness of control measures against salmonellosis in each region.