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HIGH EXPRESSION OF P53 PROTEIN IN TOXOPLASMOSIS AMONG WOMEN WITH SPONTANEOUS MISCARRIAGE IN BASRAH Full text
2015
Emarah | Maysoon sharief | Ghazi Y . AL | Muntaha A. H. Nasir
Recurrent abortion is a worldwide problem, with undefined causes. Apoptosis could play a major role in the process. The Objective of the work to detect the expression of p53 protein at the materno-fetal interface in patients with recurrent pregnancy loss (RPL). Immunohistochemistry analysis of P53 protein using paraffin embedded sections of curate samples obtained from 40 women divided into three groups : 16 women with recurrent abortion as postive with toxoplasmosis,10 women with recurrent abortion negative of toxoplasmosis and14 women with no histry of abortion as control group . The mean value of the expression of P53 protein was (40.87± 7.54),which is significantly higher than that of the second group(28.2± 4.89),and the third group (13.07± 4.49). The high expression of p53 protein in women with RPL may have a role in accelerating placental apoptosis leading to failure of pregnancy.
Show more [+] Less [-]STUDY THE PROTECTIVE ROLE OF QUERCETIN AGAINST O-ANISIDINE TOXICITY ON SOME HEMATOLOGICAL PARAMETERS OF LABORATORY MALE RATS (RATTUS NORVEGICUS). Full text
2015
Wissam Sajid Al-Uboody | Muhammed Ali Al-Diwan
The study was designed to assess the protective role of Quercetin against Oanisidine toxicity. 24 male rats (Rattus norvegicus) were used and divided into 3 equal groups of 8 male rats each. The first group was the control group in which the animals were fed on a standard ration for 15 days, then they were terminated. The second group was fed on a ration contains 1000mg/kg O-anisidine hydrochloride for 15.The third group was fed on a ration contains 1000mg/kg O-anisidine hydrochloride + 80mg/kg Quercetin dihydrate for 15. The results revealed that the treatment with Oanisidine hydrochloride for 15 days (1rst treated group) caused significant decrease in the R.B.C. count, Hb concentration, P.C.V. percentage, neutrophil and lymphocyte counts and it caused significant increase in platelets count, total leukocytes, monocytes, eosinophil and basophil counts, as compared with control group. When Quercetin dihydrate was offered as a protective agent in the ration of the 2nd treated group, it showed a significant ameliorating effect by increasing the R.B.C. count, Hb concentration, P.C.V. percentage, neutrophil and lymphocyte counts and it caused significant decrease in platelets count, total leukocytes, monocytes, eosinophil and basophil counts, as compared with the 1rst treated group. For the blood indices (RDW, MCH, MCHC, MCV, and MPV) there were no significant differences among all the experiment groups except for the mean platelet volume (MPV), where Oanisidine hydrochloride caused significant decrease in the MPV of the 1rst treated group as compared with control and 2nd treated group at (P≤0.05).
Show more [+] Less [-]- EFFECT OF SEASON ON SOME HEMATOLOGICAL, BIOCHEMICAL AND SOME HORMONE OF LOCAL IRAQI BLACK FEMALE GOATS Full text
2015
Waleed Y. Kasim | Abd-Alkareem A. Babe | Murtada F. AL-Hellou
This study was conducted at animal farm / Collage of Agriculture, Basrah University, during the period from April to December . A total of 8 blood samples were taken from dry female goats at (2.5-3.5) years old during first day of April , July , October , December months .The aim of this study to evaluate the effect months of year on blood (WBC , RBC, PCV and Hb) and biochemical parameters (Fe , Phosphate, Albumin, Total protein, Cholesterol and Glucose in addition to some hormones like Estrogen and thyroxin. The present study resulted that there were significant (P
Show more [+] Less [-]DETECTION OF FOOT AND MOUTH DISEASE ـVIRUS INFECTION ASSOCIATED ANTIBODIES IN SHEEP SERA IN BASRA BY AGID TEST Full text
2015
Wessam M. Muhammed Saleh et al.,
This study was performed to detect the antibodies against the virus-infectionassociated antigen (VIAA) in previously diseased sheep in Basra. The test is valuablein epizootiological surveys because only infected animals with foot and mouth diseasevirus will give positive reaction.Seventy five sheep sera were re-tested by AGID test to VIA antibodies from241 sheep sera examined by ELISA-VIAA test, collected from 13 suspicious infectedsheep flocks with FMD from two areas in Basra.It was found -by AGID test that 56% of the total positive tested sheep sera toELISA build specific VIA antibodies against FMD virus, and that 96% of the negativeELISA samples were negative to AGID test.The results indicated that ELISA technique was more sensitive than AGIDtechnique
Show more [+] Less [-]TOXOPATHOLOGICAL STUDY OF METHAMYL EFFECT ON THE ROCK PIGEONS (CULUMBA LIVIA GADDI) Full text
2015
Bushra H. Faris | Dhiaa J. Hamzah | Saif S. Rasheed | Mohammed A. Abo- Ktifa
This Study was conducted in animal house in Faculty of veterinary medicine of university of kufa. This study was designated to assess the neuropathy of Organophosphorus pesticide (Methomyl) after long–term administration of low dose in Rock pigeons. The Clinical signs, gross lesions and histopathological assessment of nervous tissue were described. A total of 20 pigeons, 3months old and about 500g average body weight pigeons were used in this study. The pigeons were equally divided in to tow groups. The dose was calculated based on study at close pilot at 0.02 ml and gradually lose the dose down to the toxic non-lethal dose. The toxic dose was 0.01 ml. One group was daily administered via oral gavages with 0.01 ml of methomyl; for 21 days where as the other group were leave control without administration. Organophosphorus pesticide treated groups exhibited both muscarinic and nicotinic signs of toxicity.
Show more [+] Less [-]EFFECT OF LOW INTENSITY PULSED ULTRASOUND ON REPAIR OF TENOTOMIZED ACHILLES TENDON OF RABBIT Full text
2015
Saddam Khalid Humadi | Hiba Abdulaziz Shekho
Tenotomized Achilles tendon Rabbits were used to investigate the effect of lowintensity pulsed ultrasound (LPUS) to accelerate the repair of injured tendon. The medial Achilles tendons of 18 local breed male rabbits were transected of all animals without suture, and bandaging was performed to immobilization the injured limb. Nine animals were chosen randomly to receive low-intensity pulsed ultrasound, the other 9 animals served as control. pulsating ultrasound with intensities 500 mW/cm2 and frequency 2.5 MHz were given over the healing tendons for 10 minute daily, using a gel as the coupling agent between the ultrasound probe and the skin. The healing tendons were evaluated clinically, macroscopically and microscopically for three periods at 7, 14 and 21 days respectively after tenotomy, three rabbits for each period. In the ultrasound-treated group at 14 and 21 days postoperatively, the macroscopic and histological studies demonstrated that the low-intensity pulsed ultrasound enhanced the healing of tendomize Achilles tendon. The ultrasound treated group showed more mature organization at all end points of injured tendons compared
Show more [+] Less [-]HISTOLOGICAL AND HISTOCHEMICAL STUDY OF THE LIVER OF IRAQI LOCAL DUCKS Full text
2015
Maha Abd Al- Hadi Al- Abdulla
The aim of study is provide basic data about the histological features of the liver of local Iraqi ducks and to study the glycogen content in the cytoplasm of the hepatocyte. The anatomical examination showed that liver laid cranial part of the abdominal cavity caudally and ventrally to the heart and associated to the gizzard. The liver of Mallard is divided into two undivided lobes right and left lobes (the right lobe is larger than left lobe). Histological examination revealed that the liver consist of several lobules separated from each other by thin trabeculae of connective tissue extend from delicate capsule that enclosed the liver. The basic unit of the parenchyma of liver is hepatocyte which arranged in plates or cords like that radiated around the central vein and between these cords there is sinusoids lined by alayer of fenestrated endothelial cells and Kupffer's cells. In the boundary of each lobule showed portal area which consist of hepatic artery; hepatic vein and bile duct lining by cuboidal cells. The histochemical study showed formed differ size of glycogen granules in cytoplasm of hepatocytes and in some speciment the glycogen large granules arranged around the central vein because the bird in starvation period .the liver of Mallard generally is closely similar to the liver of another avian birds and vertebrates
Show more [+] Less [-]PREVALENCE OF GASTROINTESTINAL NEMATODES PARASITES FROM SHEEP AND EVALUATION OF SOME ANTHELMINTHIC RESISTANCE IN ERBIL GOVERNORATE Full text
2015
Khalid Jabar Aziz | Ahmed Ibrahim Ahmed | Sherzad Othman Abdullah
Prevalence and anthelmintic resistance status of gastrointestinal nematodes in 430 sheep investigate by faecal examination was conducted in Qushtapa, Khabat, Benslawa, Salahaddin subdistricts in Erbil, from April to July 2012. The mean eggs per gram (EPG) count determined by using modified McMaster technique, showed that (40.46%) of the sheep were infested, the higher infection was 17.2% Nematodirus spp., 13.02% Strongylus spp., 4.18% Marshaligia spp., 2.79% Trichurus spp. and 3.25% with coccidia. Four farms were selected randomly each divided to control and three treatment groups: Levamisol, Albendazole and Ivermectin groups. Anthelmintic efficacy was measured after treatment dates were investigated with faecal egg count reduction test, the FECRT percentage for Levamisol in sheep ranged from 80.3 to 94.7 which show most effective in Khabat with FECRT of 94.7% while with Albendazole ranged from 75.5% to 80.2%, suspected resistant in all group, but the injectable Ivermectin in sheep ranged from 85.2 to 94.8 and show effective in Bnaslawa and Salahaddin with FECR of 94.6% and 94.8% respectively. All the anthelmintic were found to be low effective, but resistance to Albendazole was suspected. Based on the findings, it was concluded that development of anthelmintic resistance could be prevented by avoiding frequent dosing and under dosing, while strategic deworming should be practiced by both animal health workers and animal owners.
Show more [+] Less [-]EVALUATION OF THE ISOLATION AND DETECTION METHODS FOR SALMONELLA SPP. FROM EGG SHELL CONTAMINATION USING MULTIPLEX PCR. Full text
2015
Israa Adnan Ibraheam Al-Baghdady | Ashwak Bassim Jassim | Zainab Khudher Ahmed
A total of seventy four egg samples were collected from different markets sources, to investigated for Salmonellaspp. the bacteria was isolated and identified using culturing on selective media, in addition to, biochemical and serotyping by monovalent antisera. Multiplex Polymerase Chain Reaction(PCR) detection of theinvA; and DH genes was used for conformation of the identification of the Salmonella spp.. Twenty eight samples from seventy four eggs provide positive results with PCR as Salmonella spp. depending on InvA gene which is the target for the identification at the genus level.
Show more [+] Less [-]AN INVESTIGATION ON LIGHT COLOR AND STOCKING DENSITY ON SOME PRODUCTIVE PERFORMANCE OF BROILERS Full text
2015
Mudhar A. S. Abu Tabeekh | Riyad K. Mosa | Rabia J. Abbas
This study was designated to investigate the effect of light color and stocking density on some productive performance of broilers. A total of 675 Ross 308 one-dayold broiler chicks were used in this study. The birds were exposed to white light (WL) as a control , red light (RL), blue light (BL), green light (GL), and Blue – Green mix light (BGL) by a light-emitting diode system (LED) applied for 24 hours daily in separated rooms. The birds were randomly divided and housed into 9 wooden sealed pens of 1m2in three replicates for each density 12, 15 and 18 birds/m2in the room. The results showed that the broilers reared under BGL significantly increased (P< 0.05) body weight (BW) and weight gain (WG) at 1st, 3rdand 5thweek. Obviously, stocking density had no significant effect on BW and WG at 1stweek but significantly increased under 12 birds/m2at 3rdand 5thweek. Feed intake (FI) recorded a significant increase (P< 0.05) in broilers reared under WL at 1stweek, but no statistical difference was found at 3rdweek whereas, FI significantly increased (P< 0.05) in broilers reared under BL at 5thweek. The stocking density affected FI values which were significantly differed (P< 0.05) at 1st, 3rdand 5thweek under 12 birds/m2compared with other densities. The results of the study revealed a significant increase (P< 0.05) of Feed conversion ratio (FCR) in broilers reared under BL at 1stweek and RL at 3rdweek but there was no significant difference between groups at 5thweek. The results indicated a significant difference of FCR value in 18 birds/m2at 1st week but there were no significant differences at 3rdand 5thweek at different densities. In conclusion, the results of this study indicated that chickens reared under five different color lighting schedules with three bird densities showed that broilers reared under mixing blue – green light under 12 birds/m2has a significant positive effects on production performance compared with other light programs and bird densities
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