In the conditions of the Republic of Belarus there were studied the biochemical changes in blood of cows with chronic dystrophic processes in liver by an example of fatty hepatodystrophy. There was given an estimation of these changes taking into account animal physiological state (pregnancy). The metabolic profile including the enzymatic block from aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, cholinesterase, serum albumin, total cholesterin, total bilirubin and thymol test can be used in clinical and biochemical diagnostics in case of hepatic steatosis. Its changes in case of hepatic steatosis were characteriszed by increase in activity of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, decrease in activity of cholinesterase, decrease in blood serum of albumins concentration, increase in general cholesterol and high values of thymol tests. Determination of gamma-glutamyl transpeptidase influence and flocculation test on apo-beta-lipoproteins showed that they should not be included in this complex due to their small information content. In the process of estimation of changes of a metabolic profile of blood serum it wasnecessary to consider the physiological state of animals (pregnancy) because the activity of some enzymes and metabolite concentration at them could essentially differ. Taking into consideration the character of hepatic steatosis course, the studied metabolic profile can be recommended for hyposthenic current chronic activity with the considerable involvement degree of hepatocytes into the process.

In the conditions of the Republic of Belarus there was realized an experimental study of a new method treatment of calves diseased with abomasoenteritis by means of application of a preparation Ranitidin. As a result of realized research it was established that the preparation had an expressed therapeutic activity. Inclusion of Ranitidin preparation into the complex therapeutic regimen of calves sick with abomasoenteritis with the enteral feeding once in day in a dose of 0,15 g promoted the reduction of duration and decrease in severity of the disease. There were presented the results of biochemical blood indicators in trial and control groups of calves, haematologic indices in trial and control groups of calves prior to the beginning of experiment and in the end of the test.

In the conditions of the Republic of Belarus there was realized the evaluation of genetic inheritance indexes of producing bulls at a cattle breeding enterprise in Belarus depending on a selection source (in accordance with an average productivity of female ancestors) in order to use them properly for the increasing of genetic potential of black-and-white Belarusian cows in Gomel region. The carried out analysis allowed to draw a conclusion, that bulls of national breeding lines according to the indexes of cow milk yields concede foreign bulls, i.e. delivered of Sweden on 2744 kg of milk or on 27,5% and from Russia and Hungary - on 1272-2906 kg of milk or 12,7-29,1% (Р less than 0,001). In accordance with butter-fat yielding capacity of bull mothers obtained at breeding farms of Belarus and in accordance with groups delivered from the other countries there were stated no essential distinctions (0,05). Butter-fat yielding capacity percentage of bull mothers varied within 3,99-4,10%. However, the yield of dairy fat per lactation the difference was in favour of bull mothers delivered from Sweden (114 kg or 27,9%) at P less than 0,001. Distinctions on milk producing ability of bull mothers from various cattle breeding enterprises of Belarus were revealed. It was established, that at mothers of bulls delivered from Hungary the variability of milk yield was the highest (3032 kg or 23%). Low variability indexes of milk yield and butter-fat yielding capacity percentage was typical for bull mothers of the Belarusian selection. The standard deviation at them made 872 kg of milk yield with 9% variability coefficient, and 0,03% - for butter-fat yield and 7% respectively. Variability on all indicators of dairy efficiency of bull mothers from various cattle breeding enterprises of Belarus in most of the cases was low - from 2,3 to 13%.

In the conditions of the Republic of Belarus there were analyzed indices of impregnating ability and efficiency of stud bull mothers of various genotypes on a kappa-casein gene (CSN3). As a result of genotyping of kappa-casein gene by DNA-diagnostics method it is revealed three genotypes - CSN3АА, CSN3AB, CSN3BB. Frequency of occurrence of homozygous genotype CSN3AA at stud bulls - 87,3%; heterozygotic CSN3AB - 20,5%; homozygous CSN3BB - 1,2%. Statistically authentic difference between these indices depending on a genotype it is not established. Thus, authentic connection between indices of impregnating ability and efficiency of stud bull mothers and their genotype on a locus of a kappa-casein gene that specifies in absence of influence of the last on the given indices is not established and gives the chance to conduct selection on gene CSN3 without decrease in breeding value of stud bulls on reproductive qualities.

Influence of various levels of the organic form of selenium of Sel-Pleks on the formation of reproductive function of breeding bull-calves is studied in the conditions of the Republic of Belarus. Feeding of organic selenium to bull-calves promoted volume increase of ejaculate on 5,0%, quantities of male germ cells in ejaculate - on 9,1% and concentration of male germ cells in ejaculate - on 7,5% in comparison with animals in which rations sodium selenit was used. Application in winter period of the premix with inclusion of selenium in a dose of 0,4 mg per 1 kg of a dry matter of a ration allows to increase the quantity and quality of sperm of bull-calves: volume ejaculate - on 9,5%, concentration of male germ cells in ejaculate - on 16,4% (Р less than 0,05) and activity of male germ cells - on 8,9% (Р less than 0,001). Increasing of a dose of selenium up to 0,4 mg per 1 kg of a dry matter in rations of bull-calves in summer period allows to increase quantity and quality of sperm. At bull-calves of the third experimental group the ejaculate volume has increased on 4,5%, concentration of male germ cells in ejaculate - on 15,7% (Р0,05) and activity of male germ cells - on 7,3% (Р less than 0,001).

On the basis of the spent research high enough preventive efficiency of Ehrakond application, low intensive laser radiation together with a constant magnetic field is defined that allows to recommend them for wide application in the program of immunocorrection of an organism of agricultural animals. At the first stage of the research in immune deficiency when in blood of newborn calves almost there are no antibodies, influence on biologically active points of an udder of cows (intensity 10 mW/square cm, expositions of seconds) has made positive impact on increase of immune properties of colostric milk. Use of immunostimulants Ehrakond also has increased immunocompetent properties of cow milk (7,5 mg on 1 kg of body weight once a day within 15 days). At feeding of colostrum the physiological condition, growth and development of calves have considerably improved. In the second (when colostric protection factors in an organism decline, and own organism forms them insufficiently) and the third (decrease in immune reactance at changes in feeding and the maintenance of calves) immune deficiency, application of immunostimulants Ehrakond and influence of low intensive laser radiation together with a constant magnetic field on reflexogenic zones on a young growth organism has made positive impact indices of natural resistance of calves. It has led to activization of factors of natural nonspecific protection of an organism that has considerably improved a physiological condition and has increased natural immunity of an organism to diseases. However, the best results on natural resistance, efficiency and safeties of calves have been reached at an irradiation at the BAP initial stage of a mammary gland at cows by low intensive laser radiation together with a constant magnetic field, with the subsequent bottle-feeding of the irradiated colostrum to newborn calves; in 10 days of a life to calves the preparation Ehrakond was entered. | На основании проведенных исследований определена достаточно высокая профилактическая эффективность применения Эраконда, низкоинтенсивного лазерного излучения совместно с постоянным магнитным полем, что позволяет рекомендовать их для широкого применения в программе иммунокоррекции организма сельскохозяйственных животных. На первом этапе исследований в период иммунного дефицита, когда в крови новорожденных телят почти отсутствуют иммуноглобулины, воздействие на биологически активные точки вымени коров (интенсивность 10 мВт/см2, экспозиции секунд) оказало положительное влияние на повышение иммунных свойств колострального молока. Использование иммуностимулятора Эраконд также повысило иммунокомпетентные свойства молока коров (7,5 мг на 1 кг живой массы один раз в сутки в течение 15 дней). При скармливании колострального молозива значительно улучшились физиологическое состояние, рост и развитие телят. В период второго (когда колостральные факторы защиты в организме угасают, а собственный организм образует их недостаточно) и третьего (снижение иммунной реактивности при изменениях в кормлении, содержании телят) иммунного дефицита, применение иммуностимулятора Эраконд и воздействие низкоинтенсивного лазерного излучения совместно с постоянным магнитным полем на рефлексогенные зоны на теле молодняка оказало положительное влияние показатели естественной резистентности телят. Это привело к активизации факторов естественной неспецифической защиты организма, что значительно улучшило физиологическое состояние и повысило естественную невосприимчивость организма к болезням. Однако, наилучшие результаты по естественной резистентности, продуктивности и, сохранности телят были достигнуты при облучении на начальном этапе БАТ молочной железы у коров низкоинтенсивным лазерным излучением совместно с постоянным магнитным полем, с последующим выпаиванием облученного молозива новорожденным телятам; через 10 дней жизни телятам вводили препарат Эраконд.

In the conditions of the Republic of Belarus as a result of task-specific selection and breeding work there was developed and approbated Berezinsky breed type of Belarusian meaty breed. Sows of the studied breed type were notable for high indices of reproductive signs: the average multiple pregnancy in three breeding swine herd was 11,1 pigs per farrowing; milkability - 55 kg, litter size at weaning stage at 35-day age - 87,1 kg. The predominancy over the forecast exponent indices made 4,7-5,7%. In course of the study there were analyzed the forecasted indexes of the basic breeding signs of Berezinsky breed type of Belarusian meaty breed for 2005-2010; productivity indices of the experimental sows; coefficient of variations of sow productivity.

In the conditions of the Republic of Belarus there was realized a comparative study of meat productivity of mongrel Gereford х black-and-white heifers ad purebred black-and-white heifers in the conditions of different levels of concentrated feedstuff in cattle diets; and determine the most efficient and long-term method of animal breeding for meat. Research results showed that breeding of meaty of hybrids of Gereford х black-and-white heifers of the second generation without using the feeding concentrates in their diets or on using rations with a low rate of feeding concentrates (10-20%) proved to be more effective, than breeding of purebred black-and-white herdmates (for which specific density of the concentrated forages in diets made up 20% through pregnancy). Hybrids on Gereford breed in accordance with meat productivity level surpassed the purebred black-and-white animals. The most considerable results were marked at hybrid heifers with relative density of concentrates in a ration of 20% which in accordance with slaughter weight indexes surpassed the herdmates of black-and-white breed on 39,1 kg or on 23,68%, in accordance with slaughter yield - on 8,2%, in accordance with fresh carcass weight - on 37,9 kg or on 23,8%, in accordance with weight of inner porc - on 1,19 kg or on 20,62%.

In the conditions of the Republic of Belarus there was determined the productivity and foraging advantages of fodder galega (Galega officinalis) in comparison with the traditional perennial leguminous grasses (alfalfa (Medicago sativa); alsike clover (Trifolium hybridum); cookshead (Onobrychis viceaefolia)) and influence of the analyzed crop of state and internal microbiota of agricultural animals. There was studied the qualitative structure of galega in accordance with the phases of crop development and its influence on physiological functions of animals. It is established, that in the conditions of northern region of Belarus the fodder galega provided the yielding capacity of dry matter of 11,3 t/ha that exceeded the indices of alfalfa on 13,9%, alsike clover - in 2,7 times and cookshead - in 2,1 times. By the third decade of May it formed 18-20 tons green mass that made it possible to feed animals on 10-15 days earlier, than with other leguminous grasses. High degree of leafage (65,7%) preconditioned its high nutritional value. Fodder galega has high level of calcium, potassium, phosphorus and some microelements: copper, zinc, manganese, cobalt. Studying of vitamin value of fodder galega showed that its green mass was vitamin-rich, had enough of irreplaceable amino acids. It was established, that hay of fodder galega had high level of the non-decomposed protein (68,6-71%) that was very important for feeding of highly productive cows. It increased the level of crude protein in experimental animals on 8%.

In the conditions of the Republic of Belarus there was created an adhesive antigen on the basis of bacterial suspension of Escherichia which were separated from culture media by means of purification and concentration on membranes UMP-300 and UMP-5 that made it possible to obtain a cleaned-up preparation the specific activity of which was in 2-4 higher that of the unpurified one. In course of the study there was developed a method of purification and concentration of adhesive Escherichia antigens with the help of membrane technology. There were obtained biologically active adhesive antigens E.coli K88, K99, F41, 987P; there were selected the ultrafiltration membranes providing the optimal selectivity and productivity of the process; there were studied the influence of some factors on the process of adhesins concentration by means of ultrafiltration method. During the experiments there was analysed the agglutinating activity of antigents prepared by different methods.