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Pathogenicity and immunosuppressive properties of GM-97 strain of infectious bursal disease virus in commercial broiler chickens Full text
2014
Murmu, R | Islam, MN | Juli, MSB | Khan, MAS | Harun-ur-Rashid, SM | Hossain, FMA | Rahman, MM
The current study was conducted to evaluate the pathogenicity and immunosuppressive effects of GM-97 strain of infectious bursal disease virus in commercial broiler chickens. A total of 500 broiler chickens were vaccinated with the virus through oral route at 10 and 17 days of age (102-103 EID50/dose). Chickens were also vaccinated with Newcastle disease virus (Hitchner B1) orally at 14 and 21 days old. Chickens were euthanized (at 12, 14, 16, 20, 23, 26 days of age) after measuring body weight. Bursa of Fabricius was examined for any gross lesion, weighed and processed for histological investigations. Bursa to body weight ratio and bursal lesion scoring were made to evaluate pathogenicity of the virus. Blood samples were analyzed for antibody response to ND vaccine virus using HI test. Results showed that the GM-97 strain of IBDV induced mild to moderate depletion of lymphoid cells in the center of bursal follicles and non-significant difference in bursa to body weight ratio amongst vaccinated and unvacci-nated chickens. Chickens responded well to ND vaccine by mounting high level of serum NDV specific HI antibody titers. It can be concluded from the present study that GM-97 strain of IBDV has mild pathogenicity but is not immunosuppressive.
Show more [+] Less [-]Fatty acid composition, anti-inflammatory and analgesic activities of Hibiscus sabdariffa Linn. seeds Full text
2014
Sumaia Awad Elkariem Ali | Abdelwahab Hassan Mohamed | Galal Eldin Elazhari Mohammed
Hibiscus (H.) sabdariffa (commonly known as “Karkadeh” in Arabic) is widely used in various pharmacological applications in Sudan. The present study was carried out to investigate the anti-inflammatory and analgesic activities of H. sabdariffa seed extracts using rat models. In acute anti-inflammatory models, oral administration of petroleum ether extract of H. sabdariffa seeds inhibited the hind paw edema (p<0.01) which was induced by carrageenan. The petroleum ether extract exhibited significant (p<0.01) inhibition of vascular permeability in rats induced by intraperitoneal injection of acetic acid (0.6%). In cotton pellet granuloma method, the petroleum ether extract of H. sabdariffa seed showed significant inhibition of granuloma. The extract reduced (p<0.001) abdominal constrictions which was induced by injection of acetic acid (0.7%). Analysis of seed oil of H. sabdariffa using Gas Chromatography revealed the presence of three fatty acids; these were linolelaidic acid, arachidic acid, and palmitic acid. In conclusion, H. sabdariffa seeds possess anti-inflammatory and analgesic activities in rat model.
Show more [+] Less [-]Radio-diagnosis of fecoliths and its non-surgical management in dogs Full text
2014
Khan, A | Gazi, MA | Bashir, S | Dumralia, DM | Bagate, MS
Radio-diagnosis of fecoliths and its non-surgical management in dogs Full text
2014
Khan, A | Gazi, MA | Bashir, S | Dumralia, DM | Bagate, MS
Two cases with a history of cessation of feces for 2 weeks, depression, lethargy and anorexia in two different dogs; a Labrador male and a Doberman female, were presented. On physical examination, abdominal distension along with intense pain was observed. Blood samples were collected from the dogs for hematological and biochemical analyses. The examination revealed an increased percentage of packed cell volume (PCV). By radiological examina-tion, radiopaque material was seen in the large intes-tinal tract. The condition was suggestive of fecolith. The cases were managed by means of non-surgical management. Through an 11-day long medicinal treatment, the dogs recovered uneventfully.
Show more [+] Less [-]Radio-diagnosis of fecoliths and its non-surgical management in dogs Full text
2014
Arif Khan | Mohsin Ali Gazi | Suhani Bashir | D.M. Dumralia | M.S. Bagate
Two cases with a history of cessation of feces for 2 weeks, depression, lethargy and anorexia in two different dogs; a Labrador male and a Doberman female, were presented. On physical examination, abdominal distension along with intense pain was observed. Blood samples were collected from the dogs for hematological and biochemical analyses. The examination revealed an increased percentage of packed cell volume (PCV). By radiological examination, radiopaque material was seen in the large intestinal tract. The condition was suggestive of fecolith. The cases were managed by means of non-surgical management. Through an 11-day long medicinal treatment, the dogs recovered uneventfully.
Show more [+] Less [-]Milk Ring Test for spot identification of Brucella abortus infection in single cow herds Full text
2014
Najibullah Mohamand | Lakshmanasami Gunaseelan | Bharathy Sukumar | Kannan Porteen
In this study, milk samples were collected from 109 dairy cows to detect antibodies against Brucella (B.) using Milk Ring Test (MRT). Overall, 18.35% (n=20/109) of the milk samples were positive by MRT. The cows were divided into three groups based on lactation number viz., 1st, 2nd to 4th and ≥5th lactations; the prevalence of brucellosis in the groups were found to be 0.92% (n=1/109), 15.60% (n=17/109) and 1.83% (n=2/109), respectively. Considering simplicity and cost effectiveness, the MRT can be used for the preliminary screening of B. abortus infection especially in single cow herds.
Show more [+] Less [-]Seroprevalence of Neospora caninum and Toxoplasma gondii infections in aborted cattle in Hamedan, Iran Full text
2014
Jamal Gharekhani
The aim of current study was to assess the seroprevalence of Neospora (N.) caninum and Toxoplasma (T.) gondii infections in aborted cattle and subsequent estimation of role of these parasites in cattle abortion in Hamedan province, Iran. Blood samples (n=85) of aborted cattle were collected from different locations of Hamedan province for a period of two years from January, 2010 to December, 2012. All the samples were evaluated for the presence of IgG-antibodies against N. caninum and T. gondii using Enzyme Linked Immuno Sorbent Assay (ELISA). The results showed that 61.2% (n=52/85), 5.9% (n=5/85) and 3.5% (n=3/85) animals were positive for N. caninum, T. gondii and co-infection of these two, respectively. There was no significant correlation between seroprevalence rates, age groups and breeding (p>0.05). However, a significant difference was found between T. gondii infection and the type of cattle (p=0.05), unlike to N. caninum (p=0.52). This is the first report of N. caninum and T. gondii co-infection in cattle in Iran. Although the rate of T. gondii infection was lower but this parasite might partly be responsible to be transmitted to humans. Further investigations and appropriate control strategies in management of cattle farms are highly recommended.
Show more [+] Less [-]Experimental fracture healing with external skeletal fixation in a pigeon ulna model Full text
2014
Ahmed Tunio | Abu Jalila | Goh Yong Meng | Intan Shameha
This study was conducted to assess the healing of transverse ulna fracture when fixed with external skeletal fixation (ESF) in pigeon. Ulna fracture was created under Isoflurane anesthesia in pigeons. A total of 12 pigeons were used in this study, and they were divided into three equal groups: Group-1, Group-2 and Group-3. The birds of the three groups were sacrificed at 3, 6, and 12 weeks after successful fracture surgery. It was found that bone union occurred between three and six weeks in all groups. Bone alignment, and callus remodeling were evident at 12 weeks (p<0.05) post fracture fixation. There were no radiographic evidence of callus remodeling in any bird at 3 and 6 weeks post-surgery. The radiographic observations of fracture healing in this study have confirmed that ESF fixation is an effective method to stabilize ulna fracture in pigeons and results in faster fracture healing.
Show more [+] Less [-]Influence of dietary supplementation of chromium on the carcass traits of crossbred pigs Full text
2014
Guikinglung Pamei | P. Tensingh Gnanaraj | T. Sivakumar | K. Thilak Pon Jawahar | T. Muthuramalingam | P. Pothiappan
Influence of dietary supplementation of chromium on the carcass traits of crossbred pigs Full text
2014
Guikinglung Pamei | P. Tensingh Gnanaraj | T. Sivakumar | K. Thilak Pon Jawahar | T. Muthuramalingam | P. Pothiappan
This study was conducted to assess the influence of chromium on the carcass traits in crossbred (Large White Yorkshire X Landrace) pigs fed with swill feeding for a period of 5 months. Early-weaned crossbred piglets (n=24) were selected for this study, and the piglets were randomly divided into three equal groups; Group I, II, and III. The piglets were reared by following standard health coverage protocols. The feeds of Group I and II were supplemented with chromium in the form of chromium tripicolinate at 0.1 and 0.2 mg/kg of swill feed respectively, and Group III was kept as control. Carcass weight, carcass length, and bone percentage showed no significant difference among the three groups. However, better dressing percentage was observed in Group I (p<0.05). Liver and kidney weights were reduced in chromium supplemented groups (p<0.05). Chromium supplemented groups showed lower backfat thickness and fat percentage (p<0.01); whereas, loin eye areas and muscle percentage were increased as compared to the control group. Thus, it was concluded that chromium supplementation in feed of crossbred piglets influenced positively in their carcass traits.
Show more [+] Less [-]Influence of dietary supplementation of chromium on the carcass traits of crossbred pigs Full text
2014
Pamei, Guikinglung | Gnanaraj, P. Tensingh | Sivakumar, T. | Jawahar, K. Thilak Pon | Muthuramalingam, T. | Pothiappan, P.
This study was conducted to assess the influence of chromium on the carcass traits in crossbred (Large White Yorkshire X Landrace) pigs fed with swill feeding for a period of 5 months. Early-weaned crossbred piglets (n=24) were selected for this study, and the piglets were randomly divided into three equal groups; Group I, II, and III. The piglets were reared by following standard health coverage protocols. The feeds of Group I and II were supplemented with chromium in the form of chromium tripicolinate at 0.1 and 0.2 mg/kg of swill feed respectively, and Group III was kept as control. Carcass weight, carcass length, and bone percentage showed no significant difference among the three groups. However, better dressing percentage was observed in Group I (p<0.05). Liver and kidney weights were reduced in chromium supplemented groups (p<0.05). Chromium supplemented groups showed lower backfat thickness and fat percentage (p<0.01); whereas, loin eye areas and muscle percentage were increased as compared to the control group. Thus, it was concluded that chromium supplementation in feed of crossbred piglets influenced positively in their carcass traits.DOI: http://dx.doi.org/10.5455/javar.2014.a22J. Adv. Vet. Anim. Res., 1(3): 125-129, September 2014
Show more [+] Less [-]Serology based comprehensive study of Neospora infection in domestic animals in Hamedan province, Iran Full text
2014
Jamal Gharekhani | Heidar Heidari
Serology based comprehensive study of Neospora infection in domestic animals in Hamedan province, Iran Full text
2014
Jamal Gharekhani | Heidar Heidari
This study was conducted to determine seroprevalence of Neospora infection in cattle, sheep, horses, donkeys, and dogs in Hamedan province, Iran. Blood samples (n=2254) from the animals were collected randomly during 2009 to 2012. Sera were prepared from the collected blood samples, which were then examined for the presence of antibodies against Neospora using enzyme-linked immunosorbent assay (ELISA), Neospora modified direct agglutination test (N-MAT), and indirect fluorescent antibody test (IFAT). The seroprevalence rates of Neospora were found as 17.4% (n=245/1406) in cattle, 2.2% (n=8/358) in sheep, 40.8% (n=49/120) in horses, 52% (n=52/100) in donkeys, and 27% (n=73/270) in dogs. In this study, higher levels of Neospora infection were detected in cattle, horses, donkeys, and dogs. This is the first comprehensive study of Neospora infection in domestic animals in Iran. Further researches on molecular and bioassay studies and designing appropriate control strategies against neosporosis in Iran are necessary and strongly recommended.
Show more [+] Less [-]Serology based comprehensive study of Neospora infection in domestic animals in Hamedan province, Iran Full text
2014
Gharekhani, Jamal | Heidari, Heidar
This study was conducted to determine seroprevalence of Neospora infection in cattle, sheep, horses, donkeys, and dogs in Hamedan province, Iran. Blood samples (n=2254) from the animals were collected randomly during 2009 to 2012. Sera were prepared from the collected blood samples, which were then examined for the presence of antibodies against Neospora using enzyme-linked immunosorbent assay (ELISA), Neospora modified direct agglutination test (N-MAT), and indirect fluorescent antibody test (IFAT). The seroprevalence rates of Neospora were found as 17.4% (n=245/1406) in cattle, 2.2% (n=8/358) in sheep, 40.8% (n=49/120) in horses, 52% (n=52/100) in donkeys, and 27% (n=73/270) in dogs. In this study, higher levels of Neospora infection were detected in cattle, horses, donkeys, and dogs. This is the first comprehensive study of Neospora infection in domestic animals in Iran. Further researches on molecular and bioassay studies and designing appropriate control strategies against neosporosis in Iran are necessary and strongly recommended.DOI: http://dx.doi.org/10.5455/javar.2014.a23J. Adv. Vet. Anim. Res., 1(3): 119-124, September 2014
Show more [+] Less [-]Seroprevalence of Mycoplasma gallisepticum infection in backyard and commercial layer chickens in Bhola district, Bangladesh Full text
2014
Mahfuzul Islam | Jayedul Hassan | Md. Shahidur Rahman Khan
Seroprevalence of Mycoplasma gallisepticum infection in backyard and commercial layer chickens in Bhola district, Bangladesh Full text
2014
Mahfuzul Islam | Jayedul Hassan | Md. Shahidur Rahman Khan
This study aimed to determine the seroprevalence of Mycoplasma gallisepticum (MG) infection in the chicken population of Bhola district, Bangladesh, during the period from April 2011 to March 2012. A total of 480 blood samples from chickens were collected from different upazilas (sub-districts) of Bhola district. The sampling considered the types of chicken (backyard and commercial layer), age groups (pullet, adult and old) and seasons (summer and winter). On the basis of the serum plate agglutination test, 55.83% (n=268/480) chickens were found positive for MG. The MG infection was higher (62.5%) in backyard chickens as compared to those being reared in commercial farming systems (53.61%). With respect to age groups, the prevalence was highest in pullets (60.63%) followed by adults (55.63%) and old chickens (51.25%). Moreover, chickens reared in winter showed higher prevalence of MG (60.42%) as compared to those reared in summer (51.25%). In conclusion, MG infection is prevalent in the chicken population of Bhola district, Bangladesh. Appropriate strategies should be taken for successful prevention and control of this disease in Bangladesh.
Show more [+] Less [-]Seroprevalence of Mycoplasma gallisepticum infection in backyard and commercial layer chickens in Bhola district, Bangladesh Full text
2014
Islam, Mahfuzul | Hassan, Jayedul | Khan, Md Shahidur Rahman
This study aimed to determine the seroprevalence of Mycoplasma gallisepticum (MG) infection in the chicken population of Bhola district, Bangladesh, during the period from April 2011 to March 2012. A total of 480 blood samples from chickens were collected from different upazilas (sub-districts) of Bhola district. The sampling considered the types of chicken (backyard and commercial layer), age groups (pullet, adult and old) and seasons (summer and winter). On the basis of the serum plate agglutination test, 55.83% (n=268/480) chickens were found positive for MG. The MG infection was higher (62.5%) in backyard chickens as compared to those being reared in commercial farming systems (53.61%). With respect to age groups, the prevalence was highest in pullets (60.63%) followed by adults (55.63%) and old chickens (51.25%). Moreover, chickens reared in winter showed higher prevalence of MG (60.42%) as compared to those reared in summer (51.25%). In conclusion, MG infection is prevalent in the chicken population of Bhola district, Bangladesh. Appropriate strategies should be taken for successful prevention and control of this disease in Bangladesh.DOI: http://dx.doi.org/10.5455/javar.v1i1p11-15 J. Adv. Vet. Anim. Res., 1(1): 11-15, March 2014
Show more [+] Less [-]A case of Trypanosoma congolense savannah type infection and its management in a dog Full text
2014
Peter Kimeli | Ambrose Ngeno Kipyegon | Willy Edwin Mwangi | John Demesi Mande
A case of Trypanosoma congolense savannah type infection and its management in a dog Full text
2014
Peter Kimeli | Ambrose Ngeno Kipyegon | Willy Edwin Mwangi | John Demesi Mande
A case of Trypanosoma congolense savannah type infection in a 4-year old German shepherd dog weighing 26-kg was presented to the Small Animal Clinic, University of Nairobi, Kenya, with the history of anorexia and difficulty in breathing. The clinical manifestations were fever, pale mucous membrane, dyspnea and wasting. Blood examination revealed the existence of trypanosome parasites, and showed mild anemia. Internal Transcribed Spacer (ITS) based polymerase chain reaction confirmed the presence of Trypanosoma congolense savannah type. Along with supporting therapy, the case was successfully managed using diminazene aceturate injection (dosed at 3.5 mg/kg body weight) through intramuscular route. Complete recovery of the case was observed on day 6 of post-treatment.
Show more [+] Less [-]A case of Trypanosoma congolense savannah type infection and its management in a dog Full text
2014
Kimeli, Peter | Kipyegon, Ambrose Ngeno | Mwangi, Willy Edwin | Mande, John Demesi
A case of Trypanosoma congolense savannah type infection in a 4-year old German shepherd dog weighing 26-kg was presented to the Small Animal Clinic, University of Nairobi, Kenya, with the history of anorexia and difficulty in breathing. The clinical manifestations were fever, pale mucous membrane, dyspnea and wasting. Blood examination revealed the existence of trypanosome parasites, and showed mild anemia. Internal Transcribed Spacer (ITS) based polymerase chain reaction confirmed the presence of Trypanosoma congolense savannah type. Along with supporting therapy, the case was successfully managed using diminazene aceturate injection (dosed at 3.5 mg/kg body weight) through intramuscular route. Complete recovery of the case was observed on day 6 of post-treatment. http://dx.doi.org/10.5455/javar.2014.a33
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