The increasing number of diseases with urogenital chlamydiosis became a serious problem for the modern veterinary science. The importance of urogenital clamidiosis in the infectious pathology of animals was presented by the direct multifocal affect of urogenital system and after-effects of a disease influencing the reproductive function, as well as the potential hazard of its transformation into a source of chlamydia infections of other localization. In the conditions of the Republic of Belarus the chlamydial endometritis of cattle in the majority of cases was associated with Staphylococcus aureus in 25,7% of cows. Chlamydial endometritis of cows was usually preceded by: embryotocia (9,9%), pathological calving (4,4%), retention of placenta (14,8%), subinvolution of uterus (27,1%). The biochemical tests in trial group showed the 23% decrease of carotin on twofold decrease vitamin A. In course of the immunologic studies it was established, that dilution of A class immunoglobulins was on 31% higher in animals of a trial group in comparison with the control group. The level of IgG immunoglobulin G and immunoglobulin M slightly differed between groups

Influence of 33 imported heads Landrace breed boars of foreign selection on feeding performance and meat productivity of swine of the Belarusian Meaty breed was studied in the conditions of the Republic of Belarus for the enhancement of Belarusian breeds genealogical structure. There was stated the positive influence of Landrace Breed on feeding performance and meat productivity of mixed bred stores obtained after the introductive crossing with sows of Belarusian Meaty breed which was expressed in the predominancy of mixed bred hogs over the purebred ones at the stage of fattening performance test: at the age of reaching 100 kg body weight – on 0,7%; at average daily weight gain - on 1,5%; at feed consumption - on 1,2%. Mixed bred stores also had thinner back fat (on 0,9 mm) and bigger loin (on 0,4 square cm). There was stated the one-sided influence of boars of Landrace Breed on feeding performance and meat productivity indices of mixed breeds. It was established, that hybrids of four lines (Zaboj, Zalet, Zont and Bison) with regard to control groups showed the effect of heterosis at age of achievement of body weight of 100 kg, at a daily average weight gain and at forage consumption in the following rates: 1,5% (Р less than 0,05), 2,8% and 1,5% (Р less than 0,05) for Zaboj line; 2,3%, 4,9% and 2,1% (Р less than 0,01) for Zalet lines; 1,4%, 3% and 1,5% (Р less than 0,05) for Zont lines; 3% (Р less than 0,001), 6,7% and 2,7% for a line of Bison. In meat productivity indices there was stated the in-between character of inheritance which caused the decreasing of fat thickness and increasing of loin eye area at descendants of trial groups of lines: Zaboj, Zalet, Zenit, Zont on 0,88 mm (Р less than 0,05) and 0,6 square cm, 1,7 mm (Р less than 0,01) and 2,1 square cm (Р less than 0,001), 1,3 mm (Р less than 0,01) and 0,9 square cm, 1,4 mm

Research of the reproductive qualities of sows of different genotypes depending on duration of gestation period was realized in the conditions of an industrial pig complex of the Republic of Belarus. In course of research there were analysed 500 sows and 1991 farrowings and was stated that only 61,4% sows had gestation period in the range of physiological standards (at the average -11,5 days). In 17,8% of sows the mentioned above parameter was on 1,6 days shorter and in 1/5 of all sows under investigation it was 0,6 days longer. A higher productive performance was marked in double-breed sows of a genotype (White Large x Belarusian Meaty) with the average gestation period of 114,2 days. There were no essential deviations marked in the duration of the gestation period depending on the ordinal number of farrowing, though for the third farrowing the duration of the gestation period made 114,8 days and was 0,2-0,4 days longer as compared with the first and the second farrowings

In the research the genetic structure of populations of bulls Vitebsk of cattle breeding state farm and cows of Belarusian Black-and-White breed of Joint-Stock Company Olgovskij of Vitebsk region (Republic of Belarus) on a locus of a gene of kappa-casein was analyzed. As a result of genotyping a gene of kappa-casein by DNA-diagnostics method there were revealed three genotypes CSN3**AA, CSN3**AB, CSN3**BB. Frequency of occurrence of homozygous genotype CSN3**AA at cows has made 69,5%, at bulls of Vitebsk cattle breeding state farm - 78,3%; heterozygotic genotype CSN3**AB - 26,3% and 20,5%; homozygous genotype CSN3**BB - 4,2% and 1,2%, respectively. Frequency of CSN3**A allele in population of cows and bulls has made 0,83 and 0,88; CSN3**B - 0,17 and 0,11%, respectively. The presence of authentic influence of polymorphic variants of a gene of kappa-casein on level of milk yield and fat and protein content in cow milk was stated. The highest milk productivity in course of 305 days of lactation was observed at cows with homozygous genotype CSN3**BB (5984 kg), that was on 890 kg higher than in animals with genotype CSN3**AB (5094 kg), and on 1406 kg higher (Р less than 0,05), than at animals with CSN3**AA genotype (4578 kg). Cows with B kappa-casein allele in genome were characterized by higher fat content in milk (CSN3**AB - 3,61%, CSN3**BB - 3,72%) than animals with a CSN3**AA genotype - 3,60%. Cows with genotype CSN3**BB had higher level of protein in milk (3,28%) in comparison with the animals with CSN3**AA genotype (3,18%). In animals with CSN3**AB genotype the mentioned above index 3,19%. Research results showed that genetic variants of the gene of kappa-casein are important for selection practice as they could be used as breeding-genetic markers which could raise milk yields and protein content in milk

Research of possibility of application of sapropel (putrid mud) of the Chervonnoe lake (the Republic of Belarus) in diets of bred and milking sows as one of the ingredients of mixed fodders, as well as the evaluation of its influence of sow development and digestibility of nutritive elements were studied. Application of dry sapropel a source of energy, protein, mineral and vitamin nutritive elements for mixed fodder production rendered the positive influence on sows productive qualities: there was stated the increased number of born piglets on o,5 heads with a higher growing capacity; there was noted the increasing of coefficients of nutritive elements metabolism (crude protein – on 2,7%, crude fat – on 2,9%, free-nitrogen extracts – on 1,3%, and dietary fibre – jn 4,7%) with the simultaneous increasing of nitrogen, calcium, and phosphorous uptake by sows. Nutritional value of the analysed sapropel was 0,23 fodder units and 3,13 MJ of exchange energy per 1 kg of dry matter. Inclusion of 3% sapropel into mixed fodders for pregnant and suckling sows promoted the increasing of quantity of pigs at a birth, body weight of one pig to 3-week age and has ensured young growth with higher energy of growth. Use of sapropel in mixed fodders for sows makes active a metabolism and promotes increase of digestibility of the basic nutrients of a rations

Traditional raw materials in Belarus for mixed fodders production are wheat, barley, oats, rye, peas, lupine, sunflower and rape cakes, bran. However, productive action of these forages is limited because of presence of antinutritious factors and the raised cellulose content. In the Republic of Belarus there was organized production of national enzyme fodder additive Fekord 2004. Peculiar feature of the presented enzyme complex was addition of into its structure of enzyme glucoamylase, promoting hydrolytic starch splitting, and accordingly, the increasing of absorbency of difficult carbohydrates of forages. It was especially important for stores at early stages. Thereupon it was expedient to study the efficiency of application specified multienzyme composition in diets of growing and fattened stores. It was established, that enrichment of mixed fodder with the analysed fodder fermental additive at the rate of 0,13 and 0,15 kg per ton of mixed fodder made it possible to increase the growth rate of pigs and lower charges for forages per unit of body weight gain. Higher economic return on the fodder enzymic additive an additional gain of body weight appeared at its introduction in quantity of 0,15 kg per ton of mixed fodder

In animal feeding technology much attention is given to application of biologically active substances for metabolism stimulation. One of such biologically active substances is carnitine, an anabolic preparation of nonhormonal nature, cofactor in system of oxidation of fat acids. It participates in processes of acetylation at oxidation of fat acids together with acyl-CoA and other enzymes, promotes carrying over of cytoplasmatic Coa-derivatives of fat acids inside mitochondrions. It increases absorption speed from a digestive path of nutrients of forage, raises use of fat acids for the power purposes. Under its influence synthesis of triglycerides decreases and synthesis of phospho-lipids increases. Carnitine also protects cells against toxic accumulation of components of acyl-CoA of endogenic or exogenous origins, such as acylic groups of carnitine ethers which then can pass into liver for dissimilation or in kidneys for allocation in urine. Carnitine synthesis satisfies body requirements, but in the conditions of a hightened stress, high productivity, as well as in the conditions of lowered carnitine content in forage or in the conditions of the increased content of fats it should be applied as additive to diet. As a result of the realized study it was established, that the optimal quantity of carnitine application in diets for stores husbandry and fattening is dose of 50 g/t of mixed fodders. Application of carnitine in an optimum dose renders the positive impact on animal organism, promotes the increasing average daily weight gain on 5,1% (Р less than 0,001). Application of carnitine in diets of swine stores increases a slaughter yield on 2,1%, meat content per carcass - on 2,3%, a loin eye area - on 8,8%. Carnitine application in a dose 50 g/t of mixed fodders makes it possible to receive the additional profit at a rate of 4,57 USD per 1 head

Study of the regeneration processes and raising the efficiency of rape (Brassica napus) organs and tissues cultivation in vitro methods, as well as the achievement of a stable yield of proper regenerants were realized in the conditions of the Republic of Belarus. The obtained data specified the possibility of generation of rape finished regenerants in vitro culture without additional passages and transfers by means of effectively selected concentrations of phytohormones and biologically active substances, which made it possible to realize principles of totipotency and polarity of crop vegetative organs. On the explant part which was submerged into culture medium there was formed a root, and on opposite one - the morphogen structures. The possibility of obtaining rape regenerants from hypocotyls segments by straight regeneration was studied. Application of the analyzed substances considerably simplified and reduced the price of process of full regenerants formation, and made it possible to receive a wide spectrum of various forms that was important for selection process due to the opportunity to select valuable (in protein content) samples for the further studies and their inclusion into production process

Analysis of the efficiency of application of fermented milk supplementary feed DKM (Belarus) produced on the basis of bacteria strain Lactobacilus acidophilus 55/06 in calve diets was realized in the conditions of the Republic of Belarus. Influence of feeding with acidified with the trial milk additive on forage palatability, biochemical blood structure and calve productivity was studied; economic efficiency of DKM use for processing of whole milk and its use in rations of calves was defined. Experimental batches of DKM fermented milk supplementary feed were produced at Grodno Medical Preparations Plant (Belarus). It was issued in the sterile corked polyethylene 5, 10 and 20 liter bottles or canisters. The fermented milk supplementary feed DKM could be stored at the temperature from 0 deg С up to 15 deg С. Shelf-life – 3 months from the moment of production. The additive represents the homogeneous mass of viscous consistence of cream color of various shades with a sour-milk smell. The carried out economic calculations showed, that as a result of growing capacity increase it animals of a trial group and reception of an additional total live weight gain there was stated the 6,5% decrease of expenses for fodder unit production in the experimental group. However, due to a high cost of acidified milk the cost of fodder unit in trial group was on 6,8% higher. As a result, the cost price of 1 kg weight gain in trial group (despite high additional weight gains) was stated at the level of control group. Application of fermented milk supplementary feed DKM in calve diets made it possible to lower calf disease incidence and to increase calf productivity on 11,8% without increasing the cost price of production

Balanced complete feeding proved to be one of the major conditions of animal productivity increase and improvement of their reproductive qualities. Full value of feeding could be obtained at the expense of forage quality improvement and perfection of structure of the rations providing the increasing of metabolic energy use by breeding bull-calves. Analysis of the efficiency of metabolic energy use by the replacement bull calves under conditions of various sugar and protein ratio was realized in the Republic of Belarus. Research results showed that enrichment of replacement bull calves (with 325-405 kg of live weight) rations with easy digestible carbohydrates (sugar and starch) in ratio of 32% from a dry matter ration, including 15% of stable starch made it possible to increase the transformation of metabolic energy into energy of body weight gain on 21,73 - 23,96 MJ or on 10,3%, that as a result correspondingly increased the daily average weight gains on 7,9% and reduced the expenses for forage energy for 7,1% counting on an energy unit. According to the ejaculate volume indices the bull calves of the second experimental group surpassed analogues of a control group on 14,8%, and according to sperm concentration indices - on 12%. The average quantity of the frozen doses of sperm has made 65%