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Restriction endonuclease analysis of a porcine isolate of bovine herpesvirus type I.
1994
Varady E. | Tuboly T. | Derbyshire J.B.
Pharmacokinetic variables and bioavailability from muscle of creatine kinase in cattle Full text
1994
Lefebvre, H.P. | Toutain, P.L. | Serthelon, J.P. | Lassourd, V. | Gardey, L. | Braun, J.P. | Physiopathologie et Toxicologie Expérimentales (UPTE) ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)
International audience
Show more [+] Less [-]Identification of subspecies- and serotype1-specific epitopes on the 80to 90-kilodalton protein region of Chlamydia psittaci that may be useful for diagnosis of chlamydial induced abortion Full text
1994
Souriau, Armel | Salinas, Julio | de Sa, Carlos | Layachi, K. | Rodolakis, A. | Unité de Pathologie Infectieuse et Immunologie [Nouzilly] (PII) ; Institut National de la Recherche Agronomique (INRA)
International audience
Show more [+] Less [-]Spectrum analysis of diaphragmatic global electromyograms in cattle, with special regard to appropriate strategy for detection of fatigue. Full text
1994
Desmecht, Daniel | Linden, Annick | Close, R. P. | Michaux, C. L. | Lekeux, Pierre
peer reviewed | Although the respiratory tract of healthy and diseased cattle has been intensively studied during the past few years, only a few attempts to detect dysfunctions of bovine inspiratory muscles have been reported. Such technique would be useful in assessing the possibility of inspiratory muscle fatigue in the context of ventilatory failure. Fatigue in skeletal muscle is associated with characteristic changes in the electromyographic power spectrum. Power spectral analysis was therefore applied to cattle diaphragmatic electromyograms (EMGdi) to precisely determine the exact influence of motion and ECG artifacts, describe its basic frequency content, and extract a spectral index capable of providing an accurate warning of fatigue. The EMGdi was recorded via intramuscularly placed fishhook electrodes in 5 healthy young bulls during resting and stimulated respiration. The EMGdi and EGC signals were analyzed by use of power spectral density analysis after band-pass filtering (20 to 1,800 Hz). The EMGdi spectrum was concentrated in the band width 20 to 530 Hz. Electrode motion artifacts were absent, and it was always possible to find an electrode pair giving ECG-free EMGdi. Of the 12 power and frequency values used to quantitate the spectrum, the most stable was the centroid frequency. It was reproducible within and between calves and was only minimally altered by changing inspiratory load.(ABSTRACT TRUNCATED AT 250 WORDS)
Show more [+] Less [-]Airway occlusion pressure and diaphragm global electromyograph analysis for evaluation of inspiratory muscle drive and neuromechanical coupling in cattle Full text
1994
Desmecht, Daniel | Linden, Annick | Rollin, Frédéric | Lekeux, Pierre
peer reviewed | Although healthy and diseased bovine respiratory tracts have been intensively studied during the last years, to the authors' knowledge, there have been no attempts to objectively examine the inspiratory drive from the brain to the nerves and muscles and its transformation in pressure. Such technique would be useful in assessing the possibility of altered ventilatory drive or inspiratory muscle fatigue in the context of an animal with ventilatory failure. The relation among ventilation, airway opening occlusion pressure generated 100 milliseconds after onset of inspiration (Pawo100ms) and 6 indexes describing diaphragmatic electromyographic activity (EMGdi) recorded via implanted fishhooks was evaluated during free and impeded CO2 rebreathing in 6 young bulls. The best significant linear correlations (r > 0.8) with inspiratory center afferent stimulation, as judged by end-tidal CO2 concentration in expired air, were found for Pawo100ms, peak moving time average or variance EMGdi, and mean integrated EMGdi, whatever had been the respiratory impedance. However, with an inspiratory load, Pawo100ms responses systematically had greater increase for a given change in the driving EMGdi, implying dependence of the former not only on neural input, but also on configurational factors that determine inspiratory muscle excitation-pressure generation couplings. The reproducibility of EMGdi absolute values and changes was satisfactory up to 10 hours, but could not be repeated from one day to the other. It was concluded that, provided the constancy of the electrical coupling of the recording system to the tissue being studied is ensured, specific EMGdi and Pawo100ms values correlate reliably with amount of CO2 during free and loaded breathing.(ABSTRACT TRUNCATED AT 250 WORDS)
Show more [+] Less [-]Critical appraisal of pressure-frequency relation for estimation of diaphragm function in conscious calves Full text
1994
Desmecht, Daniel | Linden, Annick | Lekeux, Pierre
peer reviewed
Show more [+] Less [-]Quantitative Assessment of Aerial Ammonia Toxicity to the Nasal Mucosa by Use of the Nasal Lavage Method in Pigs Full text
1994
Urbain, B. | Gustin, Pascal | Prouvost, J. F. | Ansay, Michel
peer reviewed | Effects of atmospheric ammonia (NH3) on the nasal mucosa and somatic growth were investigated in pigs exposed to 4 NH3 concentrations (0; 25; 50; and 100 ppm) for 6 days in a specifically designed air-pollutant exposure chamber. Nasal lavage (NAL) was applied to quantify the ammonia-induced inflammatory response by measuring the number of neutrophils and the albumin (porcine serum albumin) concentration in the NAL liquid. In control pigs, these variables remained unchanged throughout the exposure period. In all other groups, an important ammonia concentration-related increase was recorded. The equation of the linear regression line established between the mean values of the number of neutrophils (x 10(3)) per milliliter of NAL liquid (y) recorded at the end of the exposure period and the ammonia concentrations (ppm) was: y = 69.7 + 3.3 [NH3] (r = 0.979; P < 0.020). The increase in the neutrophil count was significant (P < 0.05) at concentrations as low as 25 ppm. For albumin concentration nanograms per milliliter, the corresponding equation was: y = 574 + 14.3 [NH3] (r = 0.953; P < 0.045). However, the first significant change (P < 0.05) in this variable was only obtained for the higher concentration (100 ppm). In exposed pigs, a concentration-related depression of somatic growth was observed. The equation of the regression line plotted relating the individual values of the changes in body weight gain recorded over the exposure period expressed as percentage of the initial body weight (y) and the ammonia concentration was: y = 3.507-0.203 [NH3] + 0.001 [NH3]2 (r = 0.55; P < 0.010).(ABSTRACT TRUNCATED AT 250 WORDS)
Show more [+] Less [-]Approche directe pour la mise en évidence des phénomènes radicalaires lors de myopathie postanesthésique équine: étude préliminaire | Direct Approach for Demonstrating Free Radical Phenomena During Equine Postanesthetic Myopathy: Preliminary Study Full text
1994
Serteyn, Didier | Pincemail, Joël | Mottart, E. | Caudron, I. | Deby, Christiane | Deby-Dupont, G. | Philippart, C. | Lamy, Maurice
peer reviewed | This preliminary study demonstrated the existence of a free radical generation during an experimental postischemic muscular reperfusion in a halothane anesthetized horse. The authors used alpha-phenyl-N-tert-butylnitrone as a spin trap agent and the electronic paramagnetic resonance method to observe in vivo a free radical generation.
Show more [+] Less [-]Divisão e distribuição dos nervos frênicos no diafragma de búfalo (Bubalus bubalis Linnaeus, 1758) da raça Murrah | Division and distribution of phrenic nerves in the diaphragms of Murrah buffaloes (Bubalus bubalis Linnaeus, 1758) Full text
1994
Tocantins Neto, Arnóbio Amanajás | Ferreira, Nilson | Miglino, Maria Angélica | Souza, Wilson Machado de
Divisão e distribuição dos nervos frênicos no diafragma de búfalo (Bubalus bubalis Linnaeus, 1758) da raça Murrah | Division and distribution of phrenic nerves in the diaphragms of Murrah buffaloes (Bubalus bubalis Linnaeus, 1758) Full text
1994
Tocantins Neto, Arnóbio Amanajás | Ferreira, Nilson | Miglino, Maria Angélica | Souza, Wilson Machado de
Com base na dissecação de 30 diafragmas de búfalos, adultos, machos, da raça Murrah para estudar a divisão e a distribuição dos nervos frênicos. os autores chegaram às seguintes conclusões: os nervos frênicos terminam por bifurcação, tanto à direita (36,7%), quanto à esquerda (100,0%), ramo dorsal e tronco ventrolateral. Terminam por bifurcação somente à direita (46.7%), em ramo ventral e tronco dorsolateral. Terminam por trifurcação. apenas à direita (16,7%), em ramos dorsal, lateral e ventral. Dividem-se simetricamente (40%) por bifurcação, em ramo dorsal e tronco ventrolateral. O ramo dorsal do nervo frênico esquerdo (23,3%) apresenta distribuição heterolateral. O ramo ventral do nervo frênico esquerdo (10%) mostrou-se com distribuição heterolateral. Conexões homolaterais foram encontradas entre os ramos dorsal e lateral direitos (3,3%) entre filetes dos ramos dorsal esquerdo (3,3%), ou do lateral esquerdo (3,3%). | Based on the dissection of 30 diaphragms of adult male buffaloes of the Murrah breed, the authors studied the division and distribution of the phrenic nerves and the results found were as the follow: the phrenic nerves terminate in bifurcation both in dorsal branches and ventrolateral trunks at right (36.7%) and into left (100%) sides. The phrenic nerves terminate in bifurcation only to the right (46.7%) branches and dorsolateral trunks. The phrenic nerves terminate by trifurcation, only at the right sides (16.7%), in dorsal, lateral and ventral branches. The phrenic nerves were divided symmetrically (40%) by bifurcation into dorsal branches and ventrolateral trunks. The dorsal branches of the left phrenic nerves (23.3%) had heterolateral distribution. The ventral branches of the left phrenic nerve (10%) had heterolateral distribution. Homolateral connections were verified between the dorsal and lateral right branches (3.3%);between divisions of the left dorsal branches (3.3%) or left lateral branches (3.3%).
Show more [+] Less [-]Divisão e distribuição dos nervos frênicos no diafragma de búfalo (Bubalus bubalis Linnaeus, 1758) da raça Murrah Full text
1994
Arnóbio Amanajás Tocantins Neto | Nilson Ferreira | Maria Angélica Miglino | Wilson Machado de Souza
Com base na dissecação de 30 diafragmas de búfalos, adultos, machos, da raça Murrah para estudar a divisão e a distribuição dos nervos frênicos. os autores chegaram às seguintes conclusões: os nervos frênicos terminam por bifurcação, tanto à direita (36,7%), quanto à esquerda (100,0%), ramo dorsal e tronco ventrolateral. Terminam por bifurcação somente à direita (46.7%), em ramo ventral e tronco dorsolateral. Terminam por trifurcação. apenas à direita (16,7%), em ramos dorsal, lateral e ventral. Dividem-se simetricamente (40%) por bifurcação, em ramo dorsal e tronco ventrolateral. O ramo dorsal do nervo frênico esquerdo (23,3%) apresenta distribuição heterolateral. O ramo ventral do nervo frênico esquerdo (10%) mostrou-se com distribuição heterolateral. Conexões homolaterais foram encontradas entre os ramos dorsal e lateral direitos (3,3%) entre filetes dos ramos dorsal esquerdo (3,3%), ou do lateral esquerdo (3,3%).
Show more [+] Less [-]Biologia reprodutiva de jumentos. II. Características físicas e morfológicas do sêmen | Reproductive biology of donkey. II. Physycal and morfhologycal characteristics of semen Full text
1994
Morais, Rosana Nogueira de | Mucciolo, Raul Gastão | Viana, Wilson Gonçalves
Biologia reprodutiva de jumentos. II. Características físicas e morfológicas do sêmen | Reproductive biology of donkey. II. Physycal and morfhologycal characteristics of semen Full text
1994
Morais, Rosana Nogueira de | Mucciolo, Raul Gastão | Viana, Wilson Gonçalves
Foram estudadas as características físicas e morfológicas do sêmen de seis (06) jumentos da raça Pêga, com idade variando entre 3 e 9 anos. As colheitas de sêmen foram realizadas durante a estação de monta, numa freqüência de 2 ejaculações por semana, totalizando setenta (70) amostras. Os valores médios encontrados para os parâmetros espermáticos foram os seguintes: volume total (59,88 ± 15,54 ml), volume de gel (8,19 ± 4,07 ml), motilidade espermática total(81,93 ± 6,27%), motilidade progressiva (72,86 ± 7,20%), vigor espermático (4,63 ± 0,49), concentração espermática (444,11 ± 182,72 x 10³/mm³), total de espermatozóides por ejaculado (24,88 ± 9,59 x 109), total de defeitos espermáticos (15,60 ± 3,62%), defeitos maiores (8,55 ± 2,14%) e defeitos menores (7,04 ± 2,56%). Essas médias não diferem muito daquelas reportadas para asininos. No entanto, ao serem comparadas com dados relativos a eqüinos, observa-se a existência de diferenças em alguns aspectos. O volume total do ejaculado de jumentos é inferior, porém com menor incidência e volume de gel, portanto, a porção rica em espermatozóides apresenta praticamente o mesmo volume. Entretanto, a concentração espermática e o número total de espermatozóides são maiores do que em garanhões. Além disso, pela avaliação da motilidade e vigor espermáticos, constata-se que os jumentos apresentam, de um modo geral, ejaculados de melhor qualidade. Em programas de inseminação artificial estas características indicam a possibilidade de maior número de doses por ejaculado e melhores índices de congelabilidade. | Seminal characteristics of six donkeys of the "Pega" breed, ranging from 3 to 9 years of age were studied. Between October 1988 and April 1989, seventy (70) ejaculates were collected by using artificial vagina, twice a week. The following average values, for the parameters studied were found: total volume (59,88 ± 15.54 ml), gel volume (8,19 ± 4.07 ml), total spermatozoal motility (81,93 ± 6,27%), progressive motility (72,86 ± 7,20%), sperm vigor (4,63 ± 0,49), sperm concentration (444,11 ± 182,72 x 10³/mm³), total sperm number per ejaculate (24,88 ± 9,59 x 10 9), total abnormal cells (15,60 ± 3,62%), major defects (8,55 ± 2,14%) and minor defects (7,04 ± 2,56%). These results were quite similar to that reported in the literature for donkeys. Compared with the values found by other authors lor stallions, both species are very similar, differing only in some aspects. The total volume of jackass ejaculate was inferior, however, since the gel occurrence was much lower than in stallions, the "sperm-rich" fraction volume was approximately the same. The sperm concentration and total sperm number per ejaculate were higher for asses. Furthermore, the parameters of spermatozoal motility and vigor demonstrated that jackass semen was, almost always, of superior quality. In artificial insemination programmes, these characteristics would indicate the possibility of higher number of individual-doses per ejaculate and better freezability levels.
Show more [+] Less [-]Biologia reprodutiva de jumentos. II. Características físicas e morfológicas do sêmen Full text
1994
Rosana Nogueira de Morais | Raul Gastão Mucciolo | Wilson Gonçalves Viana
Foram estudadas as características físicas e morfológicas do sêmen de seis (06) jumentos da raça Pêga, com idade variando entre 3 e 9 anos. As colheitas de sêmen foram realizadas durante a estação de monta, numa freqüência de 2 ejaculações por semana, totalizando setenta (70) amostras. Os valores médios encontrados para os parâmetros espermáticos foram os seguintes: volume total (59,88 ± 15,54 ml), volume de gel (8,19 ± 4,07 ml), motilidade espermática total(81,93 ± 6,27%), motilidade progressiva (72,86 ± 7,20%), vigor espermático (4,63 ± 0,49), concentração espermática (444,11 ± 182,72 x 10³/mm³), total de espermatozóides por ejaculado (24,88 ± 9,59 x 109), total de defeitos espermáticos (15,60 ± 3,62%), defeitos maiores (8,55 ± 2,14%) e defeitos menores (7,04 ± 2,56%). Essas médias não diferem muito daquelas reportadas para asininos. No entanto, ao serem comparadas com dados relativos a eqüinos, observa-se a existência de diferenças em alguns aspectos. O volume total do ejaculado de jumentos é inferior, porém com menor incidência e volume de gel, portanto, a porção rica em espermatozóides apresenta praticamente o mesmo volume. Entretanto, a concentração espermática e o número total de espermatozóides são maiores do que em garanhões. Além disso, pela avaliação da motilidade e vigor espermáticos, constata-se que os jumentos apresentam, de um modo geral, ejaculados de melhor qualidade. Em programas de inseminação artificial estas características indicam a possibilidade de maior número de doses por ejaculado e melhores índices de congelabilidade.
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