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Cryptosporidium immunolabeling in paraffinembedded ileum of CD-1 neonatal mice Full text
2018
Mammeri, Mohamed | Huet, Hélène | Julien, Christine | Vallée, Isabelle | Cordonnier, Nathalie | Adjou, Karim, Tarik | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR) ; École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | École nationale vétérinaire d'Alfort (ENVA) | Phileo by Lesaffre
International audience
Show more [+] Less [-]Genotypic characterisation of Avian paramyxovirus type-1 viruses isolated from aquatic birds in Uganda. Full text
2018
Wanyana, Agnes | Mugimba, Kizito K | Bosco, Omony J | Kirunda, Halid | Nakavuma, Jessica L | Teillaud, Angelique, A. | Ducatez, Mariette | Byarugaba, Denis K | Makerere University [Kampala, Ouganda] (MAK) | Interactions hôtes-agents pathogènes [Toulouse] (IHAP) ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)
International audience | Avian paramyxovirus type-1 (APMV-1) viruses of the lentogenic pathotypes are often isolated from wild aquatic birds and may mutate to high pathogenicity when they cross into poultry and cause debilitating Newcastle disease. This study characterised AMPV-1 isolated from fresh faecal droppings from wild aquatic birds roosting sites in Uganda. Fresh faecal samples from wild aquatic birds at several waterbodies in Uganda were collected and inoculated into 9-10-day-old embryonated chicken eggs. After isolation, the viruses were confirmed as APMV-1 by APMV-1-specific polymerase chain reaction (PCR). The cleavage site of the fusion protein gene for 24 representative isolates was sequenced and phylogenetically analysed and compared with representative isolates of the different APMV-1 genotypes in the GenBank database. In total, 711 samples were collected from different regions in the country from which 72 isolates were recovered, giving a prevalence of 10.1%. Sequence analysis of 24 isolates revealed that the isolates were all lentogenic, with the typical 111GGRQGR'L117 avirulent motif. Twenty-two isolates had similar amino acid sequences at the cleavage site, which were different from the LaSota vaccine strain by a silent nucleotide substitution T357C. Two isolates, NDV/waterfowl/Uganda/MU150/2011 and NDV/waterfowl/Uganda/MU186/2011, were different from the rest of the isolates in a single amino acid, with aspartate and alanine at positions 124 and 129, respectively. The results of this study revealed that Ugandan aquatic birds indeed harbour APMV-1 that clustered with class II genotype II strains and had limited genetic diversity.
Show more [+] Less [-]Zootechnical performance of Girolando cattle at Kpinnou Breeding Farm, South-West of Benin Republic Full text
2018
Alassane, Yacoubou | Ahounou, Serge Gbênagnon | Toleba, Soumanou Seibou | Adjakpa, Achille Amour | Dotche, Ignace Ogoudanan | Houaga, Isidore | Moula, Nassim | Antoine-Moussiaux, Nicolas | Hornick, Jean-Luc | Youssao, Abdou Karim Issaka
Zootechnical performance of Girolando cattle at Kpinnou Breeding Farm, South-West of Benin Republic Full text
2018
Alassane, Yacoubou | Ahounou, Serge Gbênagnon | Toleba, Soumanou Seibou | Adjakpa, Achille Amour | Dotche, Ignace Ogoudanan | Houaga, Isidore | Moula, Nassim | Antoine-Moussiaux, Nicolas | Hornick, Jean-Luc | Youssao, Abdou Karim Issaka
peer reviewed | Objective: The aim of the study was to assess the weight and reproductive performances of Girolando cattle at Kpinnou Breeding Farm (KBF) in the south western of Benin Republic. Materials and methods: The weight performance and body measurements were taken on a total of 150 Girolando cattle. The study of the reproductive traits was based on the recorded demographic indicators between 2012-2013 of 67 cows. Results: From calving to the age of 3 months, the monthly average weight of calves did not vary with the season of calving (P>0.05). Between the 4th and 9th month, the weight varied with the birth seasons and the sex of the calf. Beyond 9th month, the calf’s average weight was the same regardless the calving season. At the age of 24 months, the highest weight (393.33 kg) was obtained during the long rainy season. The highest average weight was 398.22 Kg for males against 364.25 Kg for females at 24 months. The fertility rates ranged from 91.18% to 100% and the fecundity rate ranged from 85.29% to 103% during the study period. The apparent fertility rate was 91.18%. As for the calving and abortion rates, they were 93.55% and 6.45%, respectively. The mortality rate before weaning was 6.90% while the weaning viability was 93.10%. Calving occurred from January to June and from August to December. Conclusion: In overall, the Girolando performances are satisfactory and show a good adaptation at KBF.
Show more [+] Less [-]Zootechnical performance of Girolando cattle at Kpinnou Breeding Farm, South-West of Benin Republic Full text
2018
Yacoubou Alassane | Serge Gbênagnon Ahounou | Soumanou Seibou Toleba | Achille Amour Adjakpa | Ignace Ogoudanan Dotche | Isidore Houaga | Nassim Moula | Nicolas Antoine-Moussiaux | Jean-Luc Hornick | Abdou Karim Issaka Youssao
Objective: The aim of the study was to assess the weight and reproductive performances of Girolando cattle at Kpinnou Breeding Farm (KBF) in the south western of Benin Republic. Materials and methods: The weight performance and body measurements were taken on a total of 150 Girolando cattle. The study of the reproductive traits was based on the recorded demographic indicators between 2012-2013 of 67 cows. Results: From calving to the age of 3 months, the monthly average weight of calves did not vary with the season of calving (P>0.05). Between the 4th and 9th month, the weight varied with the birth seasons and the sex of the calf. Beyond 9th month, the calfs average weight was the same regardless the calving season. At the age of 24 months, the highest weight (393.33 kg) was obtained during the long rainy season. The highest average weight was 398.22 Kg for males against 364.25 Kg for females at 24 months. The fertility rates ranged from 91.18% to 100% and the fecundity rate ranged from 85.29% to 103% during the study period. The apparent fertility rate was 91.18%. As for the calving and abortion rates, they were 93.55% and 6.45%, respectively. The mortality rate before weaning was 6.90% while the weaning viability was 93.10%. Calving occurred from January to June and from August to December. Conclusion: In overall, the Girolando performances are satisfactory and show a good adaptation at KBF. [J Adv Vet Anim Res 2018; 5(2.000): 123-130]
Show more [+] Less [-]Ameliorative effects of Helianthus annuus against nephrotoxic, cardiac, and haematological disorders in alloxan-induced hyperglycaemia in albino rats Full text
2018
Onoja Samuel Okwudili | Udem Samuel Chukwuneke | Anaga Aruh Ottah
The study evaluated the ameliorative effects of Helianthus annuus leaf extract on nephrotoxicity, cardiac, and haematologic disorders in alloxan-induced hyperglycaemic rats.
Show more [+] Less [-]Avian poxvirus infection in Polish great tits (Parus major) Full text
2018
Kozdruń Wojciech | Styś-Fijoł Natalia | Czekaj Hanna | Indykiewicz Piotr | Sandecki Rafał | Niczyporuk Jowita Samanta
Introduction: Avian poxvirus infections are widespread in the domestic poultry population but are also reported in wild birds. In poultry, these infections cause significant economic losses, while wild birds may be a reservoir for poxvirus which affects breeding poultry. However, wild birds may also exhibit characteristic anatomopathological changes. This study concerns the infection of wild-living great tits (Parus major) with the avian poxvirus in Poland.
Show more [+] Less [-]Detection of Dinophysis species and associated okadaic acid in farmed shellfish: a two-year study from the western Mediterranean area Full text
2018
Bazzoni Anna Maria | Mudadu Alessandro G. | Lorenzoni Giuseppa | Soro Barbara | Bardino Nadia | Arras Igor | Sanna Giovanna | Vodret Bruna | Bazzardi Riccardo | Marongiu Edoardo | Virgilio Sebastiano
Diarrhoetic shellfish poisoning (DSP), an alimentary intoxication known to lead to intestinal symptoms, and caused by toxins produced by some dinoflagellates (including several Dinophysis), represents a serious threat to public health. The aim of this paper was to provide information about the occurrence and abundance of potentially toxic harmful algal species causing DSP, and the associated concentration of okadaic acid (OA) toxins. The departing assumption was that in the study area there was an increase in the presence both of Dinophysis species and OA and its derivates that could result in a risk to the health of seafood consumers.
Show more [+] Less [-]Antioxidant enzyme activities in rabbits under oxidative stress induced by high fat diet Full text
2018
Skowron Marta | Zalejska-Fiolka Jolanta | Błaszczyk Urszula | Chwalińska Ewa | Owczarek Aleksander | Birkner Ewa
The aim of this study was to investigate whether the type and form of oil (raw/non-oxidised (N) or post-frying/oxidised (O)) consumed in high-fat diets affect the oxidative status of an organism, as observed by malondialdehyde (MDA) concentration as an oxidative factor and antioxidant enzyme activity.
Show more [+] Less [-]Preliminary studies on the prevalence and genotyping of Echinococcus granulosus infection in stray dogs in Van Province, Turkey Full text
2018
Oguz Bekir | Ozdal Nalan | Kilinc Ozlem Orunc | Deger M. Serdar
Introduction:Echinococcus granulosus is a zoonotic helminth of the Taeniidae family living in the small intestines of dogs. The hydatid cyst, which is the larval form of this parasite, is observed in sheep, goat, cattle, and many other organisms including humans. It causes a disease called cystic echinococcosis. Identification of strains of E. granulosus in dogs is critical in parasite control and eradication where possible. This study aims to determine the genotype of E. granulosus eggs and prevalence of this parasite in the faeces of dogs in the Van Province using the copro-PCR method.
Show more [+] Less [-]Salmonid alphavirus (SAV) Full text
2018
Deperasińska Izabela | Schulz Patrycja | Siwicki Andrzej K.
Salmonid alphavirus (SAV), genus Alphavirus, family Togaviridae, is a single-stranded RNA virus affecting Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). It is known to be responsible for pancreas disease (PD) and sleeping disease (SD) which are increasing problems, causing high fish mortality and economic losses in the European aquaculture industry. Pancreas disease was first described in Atlantic salmon in Scotland in 1976 and a similar disease caused by the closely related sleeping disease virus was first described in rainbow trout in France. There have also been reports of salmonid alphavirus infections from other European countries, including Ireland, England, Norway, Germany, Italy, and Spain. Salmonid alphaviruses have been classified into six subtypes (SAV1–6). SAV1 and SAV4–6 cause pancreas disease in Atlantic salmon in Ireland or Scotland, SAV2 is the causative agent of sleeping disease in rainbow trout, and SAV3 has been detected in Atlantic salmon in Norway. The aim of this paper was to summarise current knowledge of infections caused by salmonid alphavirus and diagnostic methods including the newest techniques, and to briefly describe prevention from SAV infections by vaccination.
Show more [+] Less [-]Ready-to-eat meat products as a source of Listeria monocytogenes Full text
2018
Kurpas, Monika | Wieczorek, Kinga | Osek, Jacek
Ready-to-eat meat products as a source of Listeria monocytogenes Full text
2018
Kurpas, Monika | Wieczorek, Kinga | Osek, Jacek
In 2015 in the European Union member states listeriosis caused 270 deaths. Food is the route of transmission in 99% of all human infection cases. Several studies from different countries have shown that the presence of Listeria monocytogenes in food can be as high as 58.3%. One of the most important ways to protect food from these microorganisms is to prevent the spread of the bacteria at processing plants at different stages of food production chain. The ability of L. monocytogenes to survive in extreme conditions and to form biofilms on various surfaces is a significant challenge for food safety. Removal of these bacteria from niches in processing plants is difficult and requires the use of sanitisers and precise equipment cleaning. The presence of L. monocytogenes in processing environment at slaughterhouses, deli meat factories or in retail may be a reason of cross-contamination. Proper hygienic systems applied by workers in food preparing places and knowledge about different routes of spreading of these bacteria may effectively decrease the risk of food contamination. Standardised legal regulations and control of meat product manufacture should be a fundamental way to protect food from L. monocytogenes contamination.
Show more [+] Less [-]Ready-to-eat meat products as a source of Listeria monocytogenes Full text
2018
Kurpas Monika | Wieczorek Kinga | Osek Jacek
In 2015 in the European Union member states listeriosis caused 270 deaths. Food is the route of transmission in 99% of all human infection cases. Several studies from different countries have shown that the presence of Listeria monocytogenes in food can be as high as 58.3%. One of the most important ways to protect food from these microorganisms is to prevent the spread of the bacteria at processing plants at different stages of food production chain. The ability of L. monocytogenes to survive in extreme conditions and to form biofilms on various surfaces is a significant challenge for food safety. Removal of these bacteria from niches in processing plants is difficult and requires the use of sanitisers and precise equipment cleaning. The presence of L. monocytogenes in processing environment at slaughterhouses, deli meat factories or in retail may be a reason of cross-contamination. Proper hygienic systems applied by workers in food preparing places and knowledge about different routes of spreading of these bacteria may effectively decrease the risk of food contamination. Standardised legal regulations and control of meat product manufacture should be a fundamental way to protect food from L. monocytogenes contamination.
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