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High-throughput sequencing as a potential tool in the quality control of infectious bronchitis vaccines Full text
2024
Pasik Katarzyna | Domańska-Blicharz Katarzyna | Iwan Ewelina | Bomba Arkadiusz
In Europe, veterinary vaccines are strictly controlled by the Official Medicines Control Laboratories (OMCLs) of the General European OMCL Network, coordinated by the European Directorate for the Quality of Medicines & HealthCare. Despite a meticulous verification programme for immunological veterinary medicinal products (IVMPs), the products’ genomic composition has not yet been subject to evaluation in veterinary pharmacy.
Show more [+] Less [-]Application of a liposomal subunit vaccine in chickens for reduction of Campylobacter gut colonisation Full text
2024
Łasica Anna | Godlewska Renata | Gubernator Jerzy | Jakubiak-Augustyn Anna | Majewski Paweł | Wyszyńska Agnieszka
Campylobacter are the most common cause of food poisoning, which manifests itself in diarrhoea of varying severity. Additionally, because of the increasing number of people with immune deficiencies, more frequent serious complications of Campylobacter infections are being observed. The main source of infection is the consumption of contaminated poultry meat, which is a consequence of the insufficiency of current hygiene and biosecurity to control Campylobacter or eliminate it from the poultry food chain.
Show more [+] Less [-]Simultaneous determination of sulfonamides, trimethoprim, amoxicillin and tylosin in medicated feed by high performance liquid chromatography with mass spectrometry Full text
2024
Patyra Ewelina | Kwiatek Krzysztof
The article presents a rapid and simple analytical procedure for determination of four sulfonamides (sulfadiazine, sulfamerazine, sulfamethazine and sulfamethoxazole), trimethoprim, tylosin and amoxicillin in animal medicated feed.
Show more [+] Less [-]Cytokine secretion in stem cells of cattle infected with bovine leukaemia virus Full text
2024
Szczotka Maria | Kuźmak Jacek
Bovine leukaemia virus (BLV) is a Deltaretrovirus responsible for enzootic bovine leukosis, the most common neoplastic disease of cattle. It deregulates the immune system, favouring secondary infections and changes in the blood and lymphatic tissues. Blood homeostasis depends on functional haematopoietic stem cells (HSCs). Bone marrow is populated by these cells, which express CD34+ and CD35+ surface antigens and produce and release cytokines involved in the maintenance of haematopoiesis. The aim of the study was determination of the profile of cytokine production by CD34+ stem cells of cattle naturally infected with BLV.
Show more [+] Less [-]Antibiotic resistance of Gallibacterium anatis biovar haemolytica isolates from chickens Full text
2024
Kursa Olimpia | Tomczyk Grzegorz | Sieczkowska Agata | Sawicka-Durkalec Anna
Gallibacterium anatis is an opportunistic bacteria inducing a range of clinical signs in poultry. Gallibacterium anatis strains show multidrug resistance to antibacterial substances. The purpose of this study was to examine the susceptibility of G. anatis biovar haemolytica isolates collected from the respiratory, reproduction and gastrointestinal tracts of chickens to different antibiotics from various classes.
Show more [+] Less [-]The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon Full text
2024
Szymczak Bartłomiej | Junkuszew Andrzej | Patkowski Krzysztof | Szponder Tomasz | Ngoc Dominika Nguyen | Drzewiecka Beata | Sobczyńska-Rak Aleksandra | Wessely-Szponder Joanna
Macrophages are crucial immune cells that play a role in tissue repair and can exhibit pro- or anti-inflammatory behaviour based on environmental stimulation. Their functional phenotype can be affected by platelet-derived products as determined by those products’ composition. When the inflammatory response caused by implantation is excessive, it can lead to rejection of the implant. Therefore, a thorough evaluation of implant haemocompatibility is necessary to minimise undesirable consequences.
Show more [+] Less [-]The effect of coffee husks used as pellet bedding material on the intestinal barrier, immune-related gene expression and microbiota composition in the broiler chicken caecum Full text
2024
Biesek Jakub | Dunisławska Aleksandra | Kozdruń Wojciech
Using coffee husks as waste material for bedding contributes to sustainable development. A sustainable choice of bedding has also, however, to be a safe choice for poultry. The study analysed immune-related gene expression in the intestinal mucosa and indicator bacteria in caecal content collected from broiler chickens bedded on material with coffee husk addition.
Show more [+] Less [-]Analysis of the role of Dirofilaria repens macrophage migration inhibitory factors in host–parasite interactions Full text
2024
Karabowicz Justyna | Długosz Ewa | Bąska Piotr | Pękacz Mateusz | Wysmołek Magdalena Elżbieta | Klockiewicz Maciej | Wiśniewski Marcin
Dirofilaria repens is a zoonotic parasitic filarial nematode that infects carnivores and occasionally humans. Knowledge of the host–parasite molecular interactions enabling the parasite’s avoidance of the host immune response in subcutaneous dirofilariasis remains limited. Parasitic orthologues of host macrophage migration inhibitory factor (MIF) are molecules potentially involved in this process.
Show more [+] Less [-]Bacterial microbiome diversity along poultry slaughtering lines: insights from chicken carcasses and environmental sources Full text
2024
Telli Arife Ezgi | Biçer Yusuf | Telli Nihat | Sönmez Gonca | Turkal Gamze | Güzel İsmail
This study aimed to determine the bacterial diversity of chicken carcasses and their surrounding environment at various stages along a poultry slaughter line.
Show more [+] Less [-]Murine hepatic proteome adaptation to high-fat diets with different contents of saturated fatty acids and linoleic acid : α-linolenic acid polyunsaturated fatty acid ratios Full text
2024
Liput Kamila P. | Lepczyński Adam | Poławska Ewa | Ogłuszka Magdalena | Starzyński Rafał | Urbański Paweł | Nawrocka Agata | Jończy Aneta | Pierzchała Dorota | Pareek Chandra S. | Gołyński Marcin | Woźniakowski Grzegorz | Czarnik Urszula | Pierzchała Mariusz
Some health disorders, such as obesity and type 2 diabetes, are associated with a poor diet and low quality of the fat in it. The type and duration of the diet have an impact on the liver. This investigation uses the proteomic approach to identify changes in the mouse liver protein profile in adaptation to high-fat diets with different saturated fatty acid contents and linoleic acid (18:2n-6) to α-linolenic acid (18:3n-3) fatty acid ratios.
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