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Traditional Diagnosis of Eimeria spp. in Fallow Deer at Middle Parts of Iraq Full text
2020
Kareem, Safaa M. | Kawan, May H.
Traditional Diagnosis of Eimeria spp. in Fallow Deer at Middle Parts of Iraq Full text
2020
Kareem, Safaa M. | Kawan, May H.
Eimeria is an apicomplex protozoon which affects many species of birds, domestic and wild animals including deer. This is the first study in Iraq that was designed for diagnosis Eimeria spp. in deer using traditional methods. Eighty fecal samples of Fallow deer (Damadama) were collected in different provinces in the middle part of Iraq during December 2018 to the end of September 2019. Samples were examined by direct smear and flotation with Sheather's solution. The total infection rate with Eimeria spp. was 70% (56/80), in which higher infection rate was observed in female deer (78%, 39/50). The results also showed that Fawn at age<3-11 months recorded higher rate of infection (86.3%, 19/22). Eimeria infection was more prevalent in Karbala and Baghdad provinces where recorded (73.3% and 72%) respectively, sharp increase of infection recorded during March (87.5%, 7/8). In addition, four species of Eimeria (E. crandalis, E. intricate, E. parva, and E. sordida) were detected in Iraqi deer according to morphological characterization of oocysts. This study highlighted the distribution of some Eimeria species in deer and revealed the effect of some epidemiological factors on prevalence in different middle Iraq provinces.
Show more [+] Less [-]Traditional Diagnosis of Eimeria spp. in Fallow Deer at Middle Parts of Iraq Full text
2020
Safaa M. Kareem | May H. Kawan
Eimeria is an apicomplex protozoon which affects many species of birds, domestic and wild animals including deer. This is the first study in Iraq that was designed for diagnosis Eimeria spp. in deer using traditional methods. Eighty fecal samples of Fallow deer (Damadama) were collected in different provinces in the middle part of Iraq during December 2018 to the end of September 2019. Samples were examined by direct smear and flotation with Sheather's solution. The total infection rate with Eimeria spp. was 70% (56/80), in which higher infection rate was observed in female deer (78%, 39/50). The results also showed that Fawn at age<3-11 months recorded higher rate of infection (86.3%, 19/22). Eimeria infection was more prevalent in Karbala and Baghdad provinces where recorded (73.3% and 72%) respectively, sharp increase of infection recorded during March (87.5%, 7/8). In addition, four species of Eimeria (E. crandalis, E. intricate, E. parva, and E. sordida) were detected in Iraqi deer according to morphological characterization of oocysts. This study highlighted the distribution of some Eimeria species in deer and revealed the effect of some epidemiological factors on prevalence in different middle Iraq provinces.
Show more [+] Less [-]Macroscopical Anatomy and Radiographic Study of the Non-Lactating Udder of Adult Local Iraqi Cow (Bos taurus) Full text
2020
Al-Maliki, Salah H. | Atyia, Mahdi A.
Macroscopical Anatomy and Radiographic Study of the Non-Lactating Udder of Adult Local Iraqi Cow (Bos taurus) Full text
2020
Al-Maliki, Salah H. | Atyia, Mahdi A.
The purpose of this study was to describe some anatomical and radiological features of the non-lactating udder of adult local Iraqi cow. Eight udder of local breed cow clinically appeared healthy and aged between 2-5 years were obtained from a local slaughterhouse located at Baghdad province. Five udders were used for anatomical study and the rest 3 udders were used for the radiological study. The study primed to describe the macroscopic anatomy of the udder and investigate the udder’s normal blood supply using red-colored latex. The mammography method was to describe the duct system of the glandular tissue of the mammary gland which was performed using the iodine compound and barium sulphate as contrast media. The results showed that the udder was located in the inguinal region and covered by pigmented skin with fine hair and composed of four quarters. Each quarter has one teat which was long, cylindrical in shape, opened with one orifice; and internally has longitudinal folds in its mucous membrane. The blood supply of udder by the external pudendal arteries (left and right) branched into cranial and caudal mammary ones. The radiological study showed that the glandular tissue of udder was composed of complex ducal system which included the teat canal (long narrow), teat sinus (spindle-shaped cavity), lactiferous sinus (large round structure), lactiferous ducts, cistern ducts, interlobular duct, intralobular duct and alveoli (round sac like).In conclusion, this study is considered as an important contribution of describing the anatomical features, main blood supply and a pathway of the intraglandular duct system of the non-lactating udder in local cow breed which is necessary for surgical operations in several cases of the udder.
Show more [+] Less [-]Macroscopical Anatomy and Radiographic Study of the Non-Lactating Udder of Adult Local Iraqi Cow (Bos taurus) Full text
2020
Salah H. Al-Maliki | Mahdi A. Atyia
The purpose of this study was to describe some anatomical and radiological features of the non-lactating udder of adult local Iraqi cow. Eight udder of local breed cow clinically appeared healthy and aged between 2-5 years were obtained from a local slaughterhouse located at Baghdad province. Five udders were used for anatomical study and the rest 3 udders were used for the radiological study. The study primed to describe the macroscopic anatomy of the udder and investigate the udder’s normal blood supply using red-colored latex. The mammography method was to describe the duct system of the glandular tissue of the mammary gland which was performed using the iodine compound and barium sulphate as contrast media. The results showed that the udder was located in the inguinal region and covered by pigmented skin with fine hair and composed of four quarters. Each quarter has one teat which was long, cylindrical in shape, opened with one orifice; and internally has longitudinal folds in its mucous membrane. The blood supply of udder by the external pudendal arteries (left and right) branched into cranial and caudal mammary ones. The radiological study showed that the glandular tissue of udder was composed of complex ducal system which included the teat canal (long narrow), teat sinus (spindle-shaped cavity), lactiferous sinus (large round structure), lactiferous ducts, cistern ducts, interlobular duct, intralobular duct and alveoli (round sac like).In conclusion, this study is considered as an important contribution of describing the anatomical features, main blood supply and a pathway of the intraglandular duct system of the non-lactating udder in local cow breed which is necessary for surgical operations in several cases of the udder.
Show more [+] Less [-]The Relationship Between Diabetes Self-Management and Blood Glucose Control in Patients With Type 2 Diabetes Mellitus in Ulee Kareng Subdistrict, Banda Aceh Full text
2020
Saminan, Saminan | Rabbany, Naufal | Aini, Zahratul | Zulkarnain, Zulkarnain | Murzalina, Cut
The Relationship Between Diabetes Self-Management and Blood Glucose Control in Patients With Type 2 Diabetes Mellitus in Ulee Kareng Subdistrict, Banda Aceh Full text
2020
Saminan, Saminan | Rabbany, Naufal | Aini, Zahratul | Zulkarnain, Zulkarnain | Murzalina, Cut
Diabetes mellitus is a type of chronic disease that is non-transmitted. Aceh province is the province with the seventh-highest prevalence of diabetes mellitus in Indonesia at 2.4%. People with type 2 diabetes mellitus are at risk of various complications and can cause death. Self-management of diabetes is one of the things that can be done by people with diabetes mellitus in controlling various complications. This study aimed to find out the relationship between diabetes self-management and blood glucose control of type 2 diabetes mellitus patients in Ulee Kareng Subdistrict, Banda Aceh. This type of sampling method in this research was cross-sectional with the number of samples in this study as many as 88 respondents. The study was conducted using the DSMQ questionnaire filling method to assess diabetes self-management level and blood glucose level value when respondents assess blood glucose control. The results of the study obtained are 30.7% of respondents have a good level of diabetes self- management, 44.3% enough, and 25% bad. As for blood glucose control, 65.9% of respondents had uncontrolled blood glucose levels, and 34.1% controlled. The results of the chi-square test obtained a p-value of 0.000 and the spearman correlation test obtained a p-value of 0.000, as well as a correlation coefficient of 0.492. Based on the data analysis, it can be concluded that there is a significant relationship between moderate and unidirectional levels between diabetes self-management and blood glucose control of type 2 diabetes mellitus patients in Ulee Kareng Subdistrict, Banda Aceh.
Show more [+] Less [-]The Relationship Between Diabetes Self-Management and Blood Glucose Control in Patients With Type 2 Diabetes Mellitus in Ulee Kareng Subdistrict, Banda Aceh Full text
2020
Saminan Saminan | Naufal Rabbany | Zahratul Aini | Zulkarnain Zulkarnain | Cut Murzalina
Diabetes mellitus is a type of chronic disease that is non-transmitted. Aceh province is the province with the seventh-highest prevalence of diabetes mellitus in Indonesia at 2.4%. People with type 2 diabetes mellitus are at risk of various complications and can cause death. Self-management of diabetes is one of the things that can be done by people with diabetes mellitus in controlling various complications. This study aimed to find out the relationship between diabetes self-management and blood glucose control of type 2 diabetes mellitus patients in Ulee Kareng Subdistrict, Banda Aceh. This type of sampling method in this research was cross-sectional with the number of samples in this study as many as 88 respondents. The study was conducted using the DSMQ questionnaire filling method to assess diabetes self-management level and blood glucose level value when respondents assess blood glucose control. The results of the study obtained are 30.7% of respondents have a good level of diabetes self- management, 44.3% enough, and 25% bad. As for blood glucose control, 65.9% of respondents had uncontrolled blood glucose levels, and 34.1% controlled. The results of the chi-square test obtained a p-value of 0.000 and the spearman correlation test obtained a p-value of 0.000, as well as a correlation coefficient of 0.492. Based on the data analysis, it can be concluded that there is a significant relationship between moderate and unidirectional levels between diabetes self-management and blood glucose control of type 2 diabetes mellitus patients in Ulee Kareng Subdistrict, Banda Aceh.
Show more [+] Less [-]Effect of Cultural-Based Breast Self-Examination Educational Program on BSE Self Efficacy Among Nursing Student, Indonesia Full text
2020
Juanita, Juanita | Jittanoon, Piyanuch | Boonyasopun, Umaporn
Effect of Cultural-Based Breast Self-Examination Educational Program on BSE Self Efficacy Among Nursing Student, Indonesia Full text
2020
Juanita, Juanita | Jittanoon, Piyanuch | Boonyasopun, Umaporn
The clinical breast self-examination is one of the strategies to prevent breast cancer to women. Previous study was conducting to promote breast self-examination, but the study did not consider about the role of culture on health behavior. The purpose of this quasi-experimental design was to examine the effect of cultural-based breast self-examination (BSE) educational program on BSE self-efficacy among nursing students. The cultural-based BSE educational program was developed based on Banduras Social Cognitive Theory and Islamic Culture. A stratified proportionate random sampling method was employed to recruit nursing students at Public Nursing College, Faculty of Medicine, Syiah Kuala University in Aceh, Indonesia. Seventy-six students who met the inclusion criteria were recruited, 36 students were in the experimental group and 40 students were in the control group. The experimental group received the cultural-based BSE educational program including exploring Islamic mandate on prevention and individual responsibility in health promotion and cultural related beliefs toward BSE, health education, BSE training, and follow-up. The BSE self-efficacy scores were examined after 3 weeks of program implementation. The statistical analysis was conducted using dependent t-test and independent t-test. The results showed, the BSE self- efficacy of the nursing students in the experimental group were significantly higher than before participation ( t (35) =-5.02, p .001). Further, the BSE self-efficacy score in the experimental group was significantly higher than thecontrol group after participation the program (t (74) = 3.49, p .01). The result showed differences of the overall BSE self-efficacy and the subscale: BSE procedural efficacy (p .01), but not significant differences for the subscale: BSE barrier management efficacy (p .05). The cultural-based BSE educational program is effective in enhancing BSE self-efficacy among nursing students. The study suggests that the application of the cultural-based BSE educational program would be extended for other women and other nursing students in different areas.
Show more [+] Less [-]Effect of Cultural-Based Breast Self-Examination Educational Program on BSE Self Efficacy Among Nursing Student, Indonesia Full text
2020
Juanita Juanita | Piyanuch Jittanoon | Umaporn Boonyasopun
The clinical breast self-examination is one of the strategies to prevent breast cancer to women. Previous study was conducting to promote breast self-examination, but the study did not consider about the role of culture on health behavior. The purpose of this quasi-experimental design was to examine the effect of cultural-based breast self-examination (BSE) educational program on BSE self-efficacy among nursing students. The cultural-based BSE educational program was developed based on Bandura’s Social Cognitive Theory and Islamic Culture. A stratified proportionate random sampling method was employed to recruit nursing students at Public Nursing College, Faculty of Medicine, Syiah Kuala University in Aceh, Indonesia. Seventy-six students who met the inclusion criteria were recruited, 36 students were in the experimental group and 40 students were in the control group. The experimental group received the cultural-based BSE educational program including exploring Islamic mandate on prevention and individual responsibility in health promotion and cultural related beliefs toward BSE, health education, BSE training, and follow-up. The BSE self-efficacy scores were examined after 3 weeks of program implementation. The statistical analysis was conducted using dependent t-test and independent t-test. The results showed, the BSE self- efficacy of the nursing students in the experimental group were significantly higher than before participation ( t (35) = -5.02, p .001). Further, the BSE self-efficacy score in the experimental group was significantly higher than the control group after participation the program (t (74) = 3.49, p .01). The result showed differences of the overall BSE self-efficacy and the subscale: BSE procedural efficacy (p .01), but not significant differences for the subscale: BSE barrier management efficacy (p .05). The cultural-based BSE educational program is effective in enhancing BSE self-efficacy among nursing students. The study suggests that the application of the cultural-based BSE educational program would be extended for other women and other nursing students in different areas.
Show more [+] Less [-]The Effect of Ethanol Extract of Malacca Leaves (Phyllanthus emblica) on The Number of Fibroblast Cells in White Rats (Rattus norvegicus) Burns Wound Full text
2020
Ajirni, Ajirni | Asmilia, Nuzul | Nazaruddin, Nazaruddin | Sutriana, Amalia | Masyitha, Dian | Isa, Muhammad
The Effect of Ethanol Extract of Malacca Leaves (Phyllanthus emblica) on The Number of Fibroblast Cells in White Rats (Rattus norvegicus) Burns Wound Full text
2020
Ajirni, Ajirni | Asmilia, Nuzul | Nazaruddin, Nazaruddin | Sutriana, Amalia | Masyitha, Dian | Isa, Muhammad
Burns are caused by heat exposure, such as fire, radiation, electricity or chemicals that can damage the skin and affect the body's systems. The aim of this study was to find out the effect of the ethanol extract of Malacca leaves (Phyllanthus emblica) on the number of fibroblast cells in white rats (Rattus norvegicus) that have burned. This study used 24 white rats (Rattus norvegicus) were divided into 4 groups that smeared with aquadest as a negative control (P1), 5% ethanol extract gel of Malacca leaves (P2), 10% ethanol extract gel of Malacca leaves (P3), and positive control applied with bioplasenton gel (P4). The IIA degree burn were created by placing a 2x2 cm hot iron plate on the back of the rat for 5 seconds. The euthanasia performed to all animal and the skin samples were collected after 15 days of treatment. Then histopathological preparations were made using HE staining. The number of fibroblast cells were analyzed by ANOVA test. The average number of white rats (Rattus norvegicus) fibroblast cells that suffered burns P1 (negative control) had a number of 7 1.4 cells/visual. Whereas th e P2 group had a number of 4.2 1.58 cells/visual. This value has a significant difference with the negative control. But the P2 and P3 values (3 1.51 cells/visual) there is no significant different with the P4 value (positive control) with an average number of P4 fibroblast cells were 2 0.4 cells/visual. The results of this study concluded that the ethanol extract of malacca leaves 5% and 10% had an effect againts accelerating burns healing in white rats ( Rattus norvegicus).
Show more [+] Less [-]The Effect of Ethanol Extract of Malacca Leaves (Phyllanthus emblica) on The Number of Fibroblast Cells in White Rats (Rattus norvegicus) Burns Wound Full text
2020
Ajirni Ajirni | Nuzul Asmilia | Nazaruddin Nazaruddin | Amalia Sutriana | Dian Masyitha | Muhammad Isa
Burns are caused by heat exposure, such as fire, radiation, electricity or chemicals that can damage the skin and affect the body's systems. The aim of this study was to find out the effect of the ethanol extract of Malacca leaves (Phyllanthus emblica) on the number of fibroblast cells in white rats (Rattus norvegicus) that have burned. This study used 24 white rats (Rattus norvegicus) were divided into 4 groups that smeared with aquadest as a negative control (P1), 5% ethanol extract gel of Malacca leaves (P2), 10% ethanol extract gel of Malacca leaves (P3), and positive control applied with bioplasenton® gel (P4). The IIA degree burn were created by placing a 2x2 cm hot iron plate on the back of the rat for 5 seconds. The euthanasia performed to all animal and the skin samples were collected after 15 days of treatment. Then histopathological preparations were made using HE staining. The number of fibroblast cells were analyzed by ANOVA test. The average number of white rats (Rattus norvegicus) fibroblast cells that suffered burns P1 (negative control) had a number of 7 ± 1.4 cells/visual. Whereas th e P2 group had a number of 4.2 ± 1.58 cells/visual. This value has a significant difference with the negative control. But the P2 and P3 values (3 ± 1.51 cells/visual) there is no significant different with the P4 value (positive control) with an average number of P4 fibroblast cells were 2 ± 0.4 cells/visual. The results of this study concluded that the ethanol extract of malacca leaves 5% and 10% had an effect againts accelerating burns healing in white rats ( Rattus norvegicus).
Show more [+] Less [-]High Prevalence of Cryptosporidium meleagridis in Domestic Pigeons (Columba livia domestica) Raises a Prospect of Zoonotic Transmission in Babylon Province, Iraq Full text
2020
Altamimi, Mohammed K.A. | Al-Zubaidi, Mohammed Th. S.
High Prevalence of Cryptosporidium meleagridis in Domestic Pigeons (Columba livia domestica) Raises a Prospect of Zoonotic Transmission in Babylon Province, Iraq Full text
2020
Altamimi, Mohammed K.A. | Al-Zubaidi, Mohammed Th. S.
Cryptosporidium is one of the most common protozoan’s parasites with remarkable infectivity of a wide range of animals, including mammals and birds. Domestic pigeons (Columba livia domestica) act as a potential reservoir for several species of Cryptosporidium because they live in close proximity to humans. This study was conducted to assess the genetic diversity of Cryptosporidium in domestic pigeons in Iraq. A total of one hundred samples obtained from feces of domestic pigeons in Babylon province were included. After being exposed to microbial examination, all fecal samples were subsequently screened by nested polymerase chain reaction (PCR) for the possible recognition of Cryptosporidium species. Microscopy tests detected only 14/100 (14%) of infection with Cryptosporidium, while molecular tests detected 21/100 (21%) of the same targeted parasite. Sequencing experiments showed a high prevalence of C. meleagridis with 13/21 (61.90%), followed by C. baileyi with 7/21 (33.33%), while only one infection was detected with C. hominis (1/21) (4.76%). No co-infection with mixed Cryptosporidium spp. was observed, and sex factor was not found to affect the infection rate. In conclusion, this study informed a high prevalence of C. meleagridis in domestic pigeons than both C. baileyi and C. hominis, respectively, signifying a higher zoonotic potential of C. meleagridis between domestic pigeons and their handlers. This finding may raise more questions with regard to the increasing infectivity of C. meleagridis in human. This is the first important screening study in Iraq that uses molecular methods for the detection of Cryptosporidium in domesticated pigeons.
Show more [+] Less [-]High Prevalence of Cryptosporidium meleagridis in Domestic Pigeons (Columba livia domestica) Raises a Prospect of Zoonotic Transmission in Babylon Province, Iraq Full text
2020
Mohammed K.A. Altamimi | Mohammed Th. S. Al-Zubaidi
Cryptosporidium is one of the most common protozoan’s parasites with remarkable infectivity of a wide range of animals, including mammals and birds. Domestic pigeons (Columba livia domestica) act as a potential reservoir for several species of Cryptosporidium because they live in close proximity to humans. This study was conducted to assess the genetic diversity of Cryptosporidium in domestic pigeons in Iraq. A total of one hundred samples obtained from feces of domestic pigeons in Babylon province were included. After being exposed to microbial examination, all fecal samples were subsequently screened by nested polymerase chain reaction (PCR) for the possible recognition of Cryptosporidium species. Microscopy tests detected only 14/100 (14%) of infection with Cryptosporidium, while molecular tests detected 21/100 (21%) of the same targeted parasite. Sequencing experiments showed a high prevalence of C. meleagridis with 13/21 (61.90%), followed by C. baileyi with 7/21 (33.33%), while only one infection was detected with C. hominis (1/21) (4.76%). No co-infection with mixed Cryptosporidium spp. was observed, and sex factor was not found to affect the infection rate. In conclusion, this study informed a high prevalence of C. meleagridis in domestic pigeons than both C. baileyi and C. hominis, respectively, signifying a higher zoonotic potential of C. meleagridis between domestic pigeons and their handlers. This finding may raise more questions with regard to the increasing infectivity of C. meleagridis in human. This is the first important screening study in Iraq that uses molecular methods for the detection of Cryptosporidium in domesticated pigeons.
Show more [+] Less [-]Molecular Characterization of Infectious Bursal Disease Virus Isolated from Naturally Infected Broiler Chickens in Erbil, Iraq Full text
2020
Ahmed, Ahmed I.
Molecular Characterization of Infectious Bursal Disease Virus Isolated from Naturally Infected Broiler Chickens in Erbil, Iraq Full text
2020
Ahmed, Ahmed I.
The infectious bursal disease (IBD) is a highly contagious and immunosuppressive disease of broiler chickens and the development of a new genetic variant of the virus is responsible for major economic losses in the poultry industry. For this purpose, it was essential to isolate the molecular characterization of the virus from vaccinated broiler in Erbil, Iraq. Clinically, the infectious bursal disease is characterized by high mortality (10-15%) with hemorrhagic lesions on the breast and thigh muscles, hemorrhagic and edematous bursa of diseased chickens. In this study, the Bursa of Fabricus (BF) samples were collected between June 2018 and January 2019. Histopathological changes of the bursal sections showed existence of the cystic vacuolation of the lymphoid follicles with leukocytes infiltration as pathognomic features for IBD virus infection; and homogenates samples inoculated in chorioallantoic-membrane showed mortality in the second passage with varying degrees of hemorrhages. Agar gel precipitation test (AGPT) was positive with specific antisera. Reverse transcription polymerase chain reaction (RT-PCR) and nucleotide sequence analysis of five fragments in the hypervariable region of VP2 gene revealed transition and transversion changes. Among the five recent IBD virus isolates, the rate of identity was approximately 99% as compared with the very virulent IBD virus from Iran (ID: DQ785171.1). Phylogenetic analysis revealed that the five isolates were closely related to the Asian group with a different percentage ranged from 98-99% while it was 97% in the European group. The local isolate of the virus was registered in the Genebank under the accession number MN48052.1. In conclusion, the isolated IBDVs belong to a very virulent group. In addition, this study demonstrates the spread of this virulent virus to poultry industries in Erbil, Iraq. Further widespread surveys could help in delivering more information on the virus variability and might assist in designing novel vaccines for this pathogen.
Show more [+] Less [-]Molecular Characterization of Infectious Bursal Disease Virus Isolated from Naturally Infected Broiler Chickens in Erbil, Iraq Full text
2020
Ahmed I. Ahmed
The infectious bursal disease (IBD) is a highly contagious and immunosuppressive disease of broiler chickens and the development of a new genetic variant of the virus is responsible for major economic losses in the poultry industry. For this purpose, it was essential to isolate the molecular characterization of the virus from vaccinated broiler in Erbil, Iraq. Clinically, the infectious bursal disease is characterized by high mortality (10-15%) with hemorrhagic lesions on the breast and thigh muscles, hemorrhagic and edematous bursa of diseased chickens. In this study, the Bursa of Fabricus (BF) samples were collected between June 2018 and January 2019. Histopathological changes of the bursal sections showed existence of the cystic vacuolation of the lymphoid follicles with leukocytes infiltration as pathognomic features for IBD virus infection; and homogenates samples inoculated in chorioallantoic-membrane showed mortality in the second passage with varying degrees of hemorrhages. Agar gel precipitation test (AGPT) was positive with specific antisera. Reverse transcription polymerase chain reaction (RT-PCR) and nucleotide sequence analysis of five fragments in the hypervariable region of VP2 gene revealed transition and transversion changes. Among the five recent IBD virus isolates, the rate of identity was approximately 99% as compared with the very virulent IBD virus from Iran (ID: DQ785171.1). Phylogenetic analysis revealed that the five isolates were closely related to the Asian group with a different percentage ranged from 98-99% while it was 97% in the European group. The local isolate of the virus was registered in the Genebank under the accession number MN48052.1. In conclusion, the isolated IBDVs belong to a very virulent group. In addition, this study demonstrates the spread of this virulent virus to poultry industries in Erbil, Iraq. Further widespread surveys could help in delivering more information on the virus variability and might assist in designing novel vaccines for this pathogen
Show more [+] Less [-]Stereological and Histopathological Effects of Trigonella foenum-graecum Seeds on Rabbits Ovary Full text
2020
Dawood, Tamara N. | Al-Araj, Ahmed S. | Abood, Dhyaa Ab.
Stereological and Histopathological Effects of Trigonella foenum-graecum Seeds on Rabbits Ovary Full text
2020
Dawood, Tamara N. | Al-Araj, Ahmed S. | Abood, Dhyaa Ab.
This study aimed to investigate the impacts of the Trigonella foenum-graecum (T. foenum-graecum) seeds on the female gonad. A total of twenty local rabbits were used in this study; were divided into four groups (5 each): first group (G1) was considered as the control group. The second group (G2), third group (G3) and fourth group (G4) were fed daily1.5%, 3%, and 4.5% of T. foenum-graecum seeds respectively for 60 days (twice daily). At the end of the experiment, the animals were euthanized by diethyl ether (C2H52O). Then the abdomen was incised, and the samples of ovaries were collected and fixed by 10% neutral buffered formalin. The histological assessment was done with a paraffin embedding technique and the histological sections were stained with Hematoxylin and Eosin stain. The result showed that the numbers of primary and secondary follicles were significantly (P<0.05) decreased in G3and G4 compared with the control (G1) and G2. The numbers of Graafian follicles were significantly P<0.05 decreased G4 compared with other groups. The diameters of the primary, secondary, and Graafian follicles were significantly smaller than the other groups. The thickness of the granulosa cell layer in G3and G4 were significantly thinner than the other groups. The histological figures declared that the ovary of G2 was similar to that in G1. The histological sections of G3 and G4 were revealed marked cortical and medullary vascular congestion and focal hemorrhage; there were also marked follicular degeneration and cystic necrosis. The study concluded that the low concentration of T. foenum-graecum (fenugreek) seeds do not have any positive effect in terms of ovarian stimulation.
Show more [+] Less [-]Stereological and Histopathological Effects of Trigonella foenum-graecum Seeds on Rabbits Ovary Full text
2020
Tamara N. Dawood | Ahmed S. Al-Araj | Dhyaa Ab. Abood
This study aimed to investigate the impacts of the Trigonella foenum-graecum (T. foenum-graecum) seeds on the female gonad. A total of twenty local rabbits were used in this study; were divided into four groups (5 each): first group (G1) was considered as the control group. The second group (G2), third group (G3) and fourth group (G4) were fed daily1.5%, 3%, and 4.5% of T. foenum-graecum seeds respectively for 60 days (twice daily). At the end of the experiment, the animals were euthanized by diethyl ether (C2H52O). Then the abdomen was incised, and the samples of ovaries were collected and fixed by 10% neutral buffered formalin. The histological assessment was done with a paraffin embedding technique and the histological sections were stained with Hematoxylin and Eosin stain. The result showed that the numbers of primary and secondary follicles were significantly P< 0.05 decreased in G3and G4 compared with the control (G1) and G2. The numbers of Graafian follicles were significantly P<0.05 decreased G4 compared with other groups. The diameters of the primary, secondary, and Graafian follicles were significantly lower than the other groups. The thickness of the granulosa cell layer in G3and G4 were significantly lower than the other groups. The histological figures declared that the ovary of G2 was similar to that in G1. The histological sections of G3 and G4 were revealed marked cortical and medullary vascular congestion and focal hemorrhage; there were also marked follicular degeneration and cystic necrosis. The study concluded that the low concentration of T. foenum-graecum (fenugreek) seeds do not have any positive effect in terms of ovarian stimulation
Show more [+] Less [-]Clinical and Biochemical Profile of Iraqi Local Breed Cows During Pregnancy and Early Lactation Full text
2020
Shareef, Al-Bayati O.A. | Luaibi, Oday K.
Clinical and Biochemical Profile of Iraqi Local Breed Cows During Pregnancy and Early Lactation Full text
2020
Shareef, Al-Bayati O.A. | Luaibi, Oday K.
This study was conducted in Al-Alam region, which is located in Salah Al-Din Governorate, on first month pregnant Iraqi local cows (n-10). The follow up extended from the first month of pregnancy up to the end of the first month post-parturition during that some clinical and biochemical parameters were measured in the serum. Blood samples from the jugular vein of cows were collected monthly for the whole period of experiment and divided into four stages: early pregnancy, mid pregnancy, late pregnancy, and early lactation immediately after birth. It was observed that the temperature, respiration rate, and heart rate increased gradually and significantly (P≤0.05) with the progress of pregnancy reaching to its highest value in the last period of pregnancy and decreased after birth. The last trimester of pregnancy and the early lactation were also showed a significant decrease (P≤0.05) of the phosphorus, calcium, iron, and copper concentrations compared to the first and second trimesters of the pregnancy however, significant increases in other biochemical values (P≤0.05) were observed in urea and creatinine concentrations in the last trimester of pregnancy and early lactation. In addition to a significant decrease (P≤0.05) of the ratios of concentrations of glucose, total protein, albumin, and globulin in the last period of pregnancy compared to other periods. The decrease in the value of globulin continued until the early birth period while the study did not show any significant difference in the concentration of bilirubin between durations. Finally, values of the ALT, AST and ALP enzymes showed significant increases (P≤0.05) in their concentrations in the last period of pregnancy. It is concluded that possible changes in the biochemical parameters of the local Iraqi cows’ blood during pregnancy and early lactation are existed
Show more [+] Less [-]Clinical and Biochemical Profile of Iraqi Local Breed Cows During Pregnancy and Early Lactation Full text
2020
Al-Bayati O.A. Shareef | Oday K. Luaibi
This study was conducted in Al-Alam region, which is located in Salah Al-Din Governorate, on first month pregnant Iraqi local cows (n-10). The follow up extended from the first month of pregency up to the end of the first month post-parturituion during that some clinical and biochemical parameters were measured in the serum. Blood samples from the jugular vein of cows were collected monthly for the whole period of experiment and divided into four stages: early pregnancy, mid pregnancy, late pregnancy and early lactation immediately after birth. It was observed that the temperature, respiration rate, and heart rate increased gradually and significantly (P ≤ 0.05) with the progress of pregnancy reaching to its highest value in the last period of pregnancy and decreased after birth. The last trimester of pregnancy and the early lactation were also showed a significant decrease (P ≤ 0.05) of the phosphorus, calcium, iron and copper concentrations compared to the first and second trimesters of the pregnancy howecer, significant increasees in other biochemical values (P ≤ 0.05) were observed in urea and creatinine concentrations in the last trimester of pregnancy and early lactation. In addition to a significant decrease (P ≤ 0.05) of the ratios of concentrations of glucose, total protein, albumin and glubulin in the last period of pregnancy compared to other periods. The decrease in the value of glubulin continued until the early birth period while the study did not showed any significant difference in the concentration of bilirubin between durations. Finally, values of the ALT, AST and ALP enzymes showed significant increases (P ≤ 0.05) in thier concentrations in the last period of pregnancy. It is concluded that possible changes in the biochemical parameters of the local Iraqi cows blood during pregnancy and early lactation are existed
Show more [+] Less [-]Trichomonas gallinae Identification and Histopathological Study in Pigeon (Columba livia domestica) in Baghdad City, Iraq Full text
2020
Fadhil, Layla T. | Faraj, Azhar A. | AL-Amery, Amer M.
Trichomonas gallinae Identification and Histopathological Study in Pigeon (Columba livia domestica) in Baghdad City, Iraq Full text
2020
Fadhil, Layla T. | Faraj, Azhar A. | AL-Amery, Amer M.
Trichomonas gallinae causes avian trichomoniasis, which is one of the most common protozoan infections in birds worldwide. Therefore, this study was conducted to investigate and identify the Trichomonas gallinae in domestic pigeons (Columba livia domestica) by microscopic examination (direct smear and Giemsa stain) and histopathological examination in Baghdad city, Iraq, during the period from beginning of October 2018 to end of March 2019. Giemsa-stained cytoplasm with light purple and nucleus with dark purple, clarification of flagella, nucleus, and cytoplasm very well. Histopathological findings of infected birds showed gross existence of yellowish white caseous necrotic material in the oral cavity and esophagus. The histopathological examination in the larynx, esophagus, trachea, crop, liver, and lung as infiltration of inflammatory cell mainly (heterophils); thickening of mucosa because of extensive infiltration of heterophils and disruption of esophageal gland; the thickness in bronchi wall of lung due to glandular hyperplasia and muscular fibroplasia, in liver focal necrosis of parenchyma with mononuclear cell (MNCs) infiltration and granuloma composed of MNCs and heterophils. The current study may contribute to determining the histopathological changes of esophagus, trachea, crop, and liver of trichomoniasis- infected pigeons.
Show more [+] Less [-]Trichomonas gallinae Identification and Histopathological Study in Pigeon (Columba livia domestica) in Baghdad City, Iraq Full text
2020
Layla T. Fadhil | Azhar A. Faraj | Amer M. AL-Amery
Trichomonas gallinae causes avian trichomoniasis, which is one of the most common protozoan infections in birds worldwide. Therefore, this study was conducted to investigate and identify the Trichomonas gallinae in domestic pigeons (Columba livia domestica) by microscopic examination (direct smear and Giemsa stain) and histopathological examination in Baghdad city, Iraq, during the period from beginning of October 2018 to end of March 2019. Giemsa-stained cytoplasm with light purple and nucleus with dark purple, clarification of flagella, nucleus, and cytoplasm very well. Histopathological findings of infected birds showed gross existence of yellowish white caseous necrotic material in the oral cavity and esophagus. The histopathological examination in the larynx, esophagus, trachea, crop, liver, and lung as infiltration of inflammatory cell mainly (heterophils); thickening of mucosa because of extensive infiltration of heterophils and disruption of esophageal gland; the thickness in bronchi wall of lung due to glandular hyperplasia and muscular fibroplasia, in liver focal necrosis of parenchyma with mononuclear cell (MNCs) infiltration and granuloma composed of MNCs and heterophils. The current study may contribute in determining the histopathological changes of esophagus, trachea, crop, and liver of trichomoniasis- infected pigeons.
Show more [+] Less [-]