This study was conducted on 180 blood samples collected randomly from equids (106 horses and 74 donkeys) in Nineveh governorate, Iraq, including, 25 clinically and laboratory healthy equids that served as control equids. Clinical examinations for all equids have been done, and samples of blood were collected from all equids for serodiagnosis, hemato-biochemical parameters examination. Results demonstrated that the total prevalence of Equine anaplasmosis (AE) in equids was 46.1% (83/180), representing 30.1% in horses and 68.9% in donkeys using indirect-ELISA. Infected equids were suffering from fever, paleness, petechial hemorrhages and/or jaundice of the mucous membranes, respiratory disorders, edema of the limbs, nervous signs, and the presence of ticks on the different parts of the body. There was a a significant increase in the body temperature, respiratory rate, heart rate, and capillary refilling time in infected equids. Hemogram revealed a significant decrease in the TRBCs, Hb, PCV, MCH, and MCHC, with a significant increase in MCV, reflecting a macrocytic hypochromic type of anemia. In addition to significant increase in the ESR. Furthermore, a significant decrease in PLT and TWBCs as a result of a significant decrease in neutrophils and lymphocytes in the infected equids compared to the control equids. Biochemical analysis showed that a significant increase in AST, ALT, ALP, TB, creatinine, and BUN with a significant decrease in TP, phosphorus and calcium, in infected equids compared to the control animal. In conclusion, The EA is widespread in equids in Nineveh Governorate, Iraq along with significant clinico-hemato-biochemical parameter alterations.

This study investigates the presence and investigate of Avian Orthoavulovirus-1 (AOAV-1), previously also called avian paramyxovirus-1, in Iraqi broiler chickens. Newcastle disease, a highly contagious and economically important poultry disease, is caused by AOAV-1. A total of 250 samples were collected from 6 Iraqi provinces; Clinically, 150 samples indicated ND. Using PCR and clinical examination, the virus was examined in 150 broiler samples from farms in the north, center and south of Iraq that clinically indicated ND. According to the results (50), 33.3% of the samples tested were positive for PCR detection using the F and HN genes.The results of this investigation may have implications for regional disease control strategies and advance our understanding of the occurrence of AOAV-1 in Iraqi poultry.

Natural treatment options for Microsporum canis dermatophytosis are being explored because of resistance to several antifungal medications. In this study, the potential antifungal effect of bee venom (BV), a natural antimicrobial agent, on M. canis was investigated. The antifungal effects of BV, fluconazole, itraconazole, amphotericin B and terbinafine were evaluated by the macrodilution method at various concentrations by modifying the microdilution method recommended by the European Committee on Antimicrobial Susceptibility Testing. All isolates were observed to be susceptible to terbinafine and fully resistant to fluconazole and amphotericin B. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of M. canis isolate 2 (Mc2) were determined as 8 µg/mL for itraconazole. The MIC and MFC values of BV were found to be 320 µg/mL for the Mc2 isolate and 640 µg/mL for the Mc6 isolate. The results showed that the isolates obtained from clinical samples in this study were highly resistant to all antifungal agents, except terbinafine. The increase in resistance indicates that antifungal drugs will become insufficient and ineffective over time and natural products such as BV should be evaluated as alternatives. Contribution: Although there are many drugs for the treatment of M. canis, the increase in resistance to antifungal agents reveals the need for the identification and development of new natural agents. Bee venom, which has been shown to have a safe and weak allergenic effect in various studies, can be tested for usability as a local antifungal drug when supported by in vivo studies.

Plant foliages used as feed additives pose a health risk due to high oxidant concentrations. Oxidants cause oxidative stress and high rate of morbidities and mortalities. Hence, the aim of the study was to validate the methods to quantify gallic acid (GA) and quercetin (Q) as putative antioxidants, and to evaluate antioxidant activities in feed (F), Hyperacanthus amoenus (HA) and Carissa bispinosa (CB) extracts. Extraction was carried out with 62.5% methanol. Method validations for linearity, accuracy and precision were performed on high performance liquid chromatography. Quantitative analysis of GA and Q and testing of 2,2-diphenyl-2-picrylhydrazyl (DPPH) scavenging activities in the extracts were performed. The lowest limit of detection (LOD) and limit of quantitation (LOQ) of 0.011 µg/mL and 0.032 µg/mL were determined in HA, respectively. The methods were accurate and precise as the relative standard deviations (%RSD) were less than 15%. The GA concentrations in CB and HA extracts were statistically significant (p  0.05) and their values were 0.65 ± 0.03 x 106 µg/kg dry weight (DW) (0.13%) and 0.28 ± 0.06 x 106 µg/kg DW (0.002%), respectively. All extracts showed very strong radical scavenging activities with their IC50 values ranging between 5.87 µg/mL and 6.86 µg/mL. Contribution: These accurate, repeatable, precise and reliable methods can be used to provide a valuable basis for GA and Q analysis in various shrub foliages. Though high GA concentrations have potential to act as antioxidants, they may have adverse health and growth performance effects when used as feed additives, while lower Q concentrations may have no effects on livestock.

To investigate the existence of bovine viral diarrhoea virus (BVDV), parainfluenza virus 3 (PIV-3) and respiratory syncytial virus (RSV) as well as its coinfections, a total of 420 pneumonic lung tissue samples were collected from slaughterhouses in three different areas. Samples were examined for the three viruses using antigen detection enzyme-linked immunosorbent assay (ELISA) test, and positive results were further confirmed using fluorescent antibody test and polymerase chain reaction. Prevalences detected were 10.47% for BVDV, 11.9% for parainfluenza virus type (PIV-3) and 7.6% for respiratory syncytial virus (RSV). Observed coinfections were bovine viral diarrhoea (BVD) and PIV-3 in 8.1%, BVD and RSV in only two samples (1%). Existence of all three viruses was detected in two samples (1%). Contribution: In this study, infection of the three viruses was confirmed in cattle and existence of its coinfection is documented for the first time.

The South African government recognises the value of traditional healing and supports an integrated healthcare system. In the veterinary field, successful integration depends on the perceptions of different animal healthcare practitioners (AHCPs). This study aimed to determine the perceptions of ethnoveterinary medicine among state-employed veterinarians and animal health technicians in the North West province. A mixed model questionnaire and non-probability sampling gathered quantitative and qualitative data from 53 participants. Statistical and thematic analysis processes were applied to quantitative and qualitative data, respectively. Overall, 77.4% of participants were aware of ethnoveterinary medicine practices. Approval of the use of ethnoveterinary medicine by farmers was above-average at a mean of 3.57, standard deviation (s.d.) = 1.016 on a 5-point Likert scale. However, 65.9% doubted the effectiveness of ethnoveterinary medicine, which suggests tolerance but no appreciation of its value. The Chi-square test revealed a significant relationship between district municipality of employment and awareness at χ2 = 17.490, df = 3, p = 0.001. Thematic analysis of comments showed that some participants perceived ethnoveterinary medicine as unscientific and interfering with Western veterinary medicine, while some considered it a good complement to Western veterinary medicine. The study also found that 20.8% of AHCPs use ethnoveterinary medicine to treat certain diseases. In conclusion, the lack of appreciation for ethnoveterinary medicine among AHCPs may hinder government-proposed integration, emphasising the need for programmes to improve perceptions among these stakeholders. Contribution: This is first such study to report on perceptions of ethnoveterinary medicine among AHCPs, and assess their readiness for an integrated animal health system.

This study aims to determine the histological and histomorphometric characteristics of the skin of domestic fowl (Gallus gallus domesticus), ducks (Anas platyrhynchos), and doves (Columba livia) in the ventral abdominal region. Each study sample consisted of six individuals. The samples were processed into histological preparations with a thickness of 5 m and then stained using Haematoxylin-Eosin (HE). The results showed that the histological structure of the skin of domestic fowl, ducks, and doves is relatively thin compared to the structure of mammalian skin and consists of the epidermis, dermis, and hypodermis (subcutis). The histomorphometric data analysis showed that the thickness of the epidermal layer of the skin in the ventral abdominal region of domestic fowl, ducks, and doves was not significantly different (P0.05), but there were significant differences (P0.05) in the thickness of the dermis and hypodermis. It can be concluded that a similarity exists in the histological structure of the skin of domestic fowl, ducks, and doves, but differences are observed in the histomorphometric properties of the skin in the ventral abdominal region of these birds.

Pediculosis capitis is an ectoparasitic disease that lives on the human scalp and caused by Pediculus humanus capitis. This incident is categorized as a neglected case, but the impact is quite significant. The incident can give a negative impact, including physical, psychological, social and school perceptions or learning impairment. This study aimed to determine the relationship between the incidence of pediculosis capitis with the quality of life of female students of Madrasah Ulumul Quran Sigli. This research is an observational analytic study with a cross-sectional approach. The samples were collected by the total sampling method. The results obtained from 155 respondents who met the inclusion criteria found that 124 respondents (80%) had pediculosis capitis and 31 respondents (20%) with no pediculosis capitis. Statistical analysis using the chi square test obtained a p value of 0.000 (0.05). The conclusion was found that there was a relationship between the incidence of pediculosis capitis and the quality of life of female students of Madrasah Ulumul Quran Sigli.

Parkinson's is a neurological disease that is rapidly increasing to surpass Alzheimer's disease. Parkinson's disease causes a decrease in the number of dopaminergic neurons, which can lead to disturbances in attention, memory, and motivation. Damage to dopaminergic nerve cells is caused by increased free radicals (ROS) levels in tissues and cells, thus inducing cell death signals. This study aims to determine whether red palm oil affects the acute level of Parkinson's syndrome in rats induced with paraquat. This study uses the BEAM and Cylinder tests to measure the effect of red palm oil because one of the alternative therapies for neurodegenerative illnesses like Parkinson's is red palm oil. The treatment was administered to paraquat-induced rats with injections given weekly for four weeks. Carotenoids are present in red palm oil at concentrations of 600 to 1000 ppm and are thought to have neuroprotective properties. As many as 30 rats were divided into three groups: K (control), PQT (paraquat 7 mg/kg body weight), and PQT+MSM (paraquat 7 mg/kg body weight + 2 ml of red palm oil). The data were then checked using the normality test Shapiro-Wilk and analyzed statistically using One-way ANOVA. The Cylinder and Beam tests were significant, with a P value of 0.00. Parkinsonian rats receiving red palm oil at a dose of 2 ml/kg body weight per week for four weeks showed improved motor function compared to those receiving only paraquat. The group with red palm oil had a lower beam test value. This indicates motor improvement in Parkinson's model rats. Similarly, the cylinder test showed higher performance in the group treated with red palm oil. Based on this, it can be concluded that red palm oil influences the motor movements of Parkinson's rats. The beam test and cylinder test methods prove this. Red palm oil could decrease the acute level of motor impairments in rats with Parkinson's induced by paraquat.

Antimicrobial Resistance (AMR) is the ability of microbes to inhibit treatment effects triggered by overuse and inappropriate use of antibiotics in poultry farms. This study aims to get an overview of the risk factors of AMR. A total of 41 E. coli isolated from caecum in poultry slaughterhouses derived from broiler chicken farms in Medan and Deli Serdang. The sample was tested for AMR using the Microbroth Dilution technique (Sensititre Microplate) with standards CLSI VET01S (2015) and CLSI M100. Information on risk factors was taken from farms using a questionnaire and analyzed with a logistic regression model (EPI Info 7 CDC). The result for sensitivity to 13 types of antibiotics showed that Trimethoprim had the highest resistance (95.1%), followed by ampicillin (90.2%), gentamicin (87.8%), nalidixide acid (85.4%), cefotaxime (82.9%), and sulfamethoxazole (82.9%). In contrast, colistin and meropenem have not shown resistance. As much as 92.7% of E. coli isolates had experienced Multi-Drug Resistance (MDR), which showed AMR to more than two antibiotic groups. The risk factor of farmer knowledge about AMR was associated with the MDR of E. coli. AMR requires the coordination of the stakeholders to carry out socialization and educate farmers in providing appropriate knowledge and using antibiotics wisely.