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Identification of Hanwoo and Holstein meat using MGB probe based real-time PCR associated with single nucleotide polymorphism (SNP) in Melanocortin 1 receptor (MC1R) gene
2005
Park, S.D. (Chonnam National University, Gwangju, Republic of Korea) | Kim, T.J. (Chonnam National University, Gwangju, Republic of Korea) | Lee, J.I. (Chonnam National University, Gwangju, Republic of Korea), E-mail: [email protected]
The melanocortin 1 receptor (MC1R) plays an important role in regulation of melanin pigment synthesis within mammalian melanocytes. Mutations within the gene encoding MC1R have been shown to explain coat color variations within several mammalian species including cattle. To develope a rapid and accurate method for the identification of Hanwoo meat, we performed a single nucleotide polymorphism (SNP) analysis in Melanocortin 1 receptor (MC1R) gene using TaqMan∨® MGB probe-based real-time PCR. Two specific probes (one for Hanwoo and the other for Holstein and Black angus) were designed.
Show more [+] Less [-]Septicemic colibacillosis accompanied by white-spotted kidney in a calf
2005
Yoon, S.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: [email protected] | Han, H.R. (Seoul National University, Seoul, Republic of Korea) | Bae, Y.C. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, H.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yang, D.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kwon, Y.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
We report septicemic colibacillosis accompanied by white-spotted kidney in a 30-day old female Holstein-Friesian dairy calf. Grossly, there were numerous white spots sized average 0.5cm in diameter in both kidneys. When sectioned sagittally, there were radially oriented gray streaks extending outward and reaching the renal cortex. The renal papillae were ulcerated, white to gray in color and very friable. Histologically, there was extensive purulent inflammation characterized by severe neutrophilic cellular infiltrations in the tubular lumens and interstitia. In addition, massive coagulative necrosis were found in the apices of papillae.
Show more [+] Less [-]Antigenetic effects of the eluted proteins from house dust mite (Dermatophagoides pteronyssinus) in dogs infested with sarcoptic mite (Sarcoptes scabiei var. canis)
2005
Kim, T.H. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, J.W. (Chungbuk National University, Cheongju, Republic of Korea) | Jee, C.H. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: [email protected]
Canine sarcoptic mite (Sarcoptes scabiei var. canis) is ectoparasite which burrow usually in the stratum corneum of the skin of dogs. Antigens from the burrowing mites induce humoral and cellmediated immune responses in the hosts. The effect of antigenecity induced by somatic antigens of house dust mite (Dermatophagoides pteronyssinus) isolated by continuous elution has been evaluated in canine sarcoptic mites infestation. Continuous elution was carried out in 7.5% SDS-PAGE to isolate proteins of common antigens from somatic antigens of house dust mite.
Show more [+] Less [-]The assessment of anesthetic depth by quantitative electroencephalography in intravenous anesthesia by intermittent bolus injection
2005
Lee, S.H. (Konkuk University, Seoul, Republic of Korea) | Bae, C.S. (Chonnam National University, Gwangju, Republic of Korea) | Noh, G.J. (University of Ulsan College of Medicine, Seoul, Republic of Korea) | Kim, J.Y. (Konkuk University, Seoul, Republic of Korea) | Chung, B.H. (Konkuk University, Seoul, Republic of Korea), E-mail: [email protected] | Bae, K.S. (University of Ulsan College of Medicine, Seoul, Republic of Korea)
To assess anesthetic depth using quantitative electroencephalography (q-EEG), we recorded processed EEG (raw EEG) till 100 minutes in beagle dogs anesthetized for 60 minutes with tiletamine/zolazepam (n=5, TZ group), xylazine/ketamine (n=5, XK group) and propofol (n=5, PI group) by intermittent bolus injection. Raw EEG was converted into 95% spectral edge frequency (SEF) and median frequency (MF) through fast fourier transformation (FFT) method. 95% SEF value of TZ group was significantly higher (p less than 0.05) than the XK group from 10 minutes to 100 minutes. 95% SEF value of PI group was significantly higher (p less than 0.05) than the XK group from 10 minutes to 40 minutes, and significantly low (p less than 0.05) than XK group at 90 and 100 minutes.
Show more [+] Less [-]The study for the mouse cerebellum developments irradiated with γ-ray during embryogenesis
2005
Park, I.K. (Chungnam National University, Daejeon, Republic of Korea) | Lee, G.J. (Chung Nam Veterinary Service Laboratory, Daejeon, Republic of Korea) | Lee, K.Y. (Daejeon University, Daejeon, Republic of Korea) | Min, T.S. (Korea Science and Engineering Foundation, Daejeon, Republic of Korea) | Byun, M.W. (Korea Atomic Energy Research Institute, Daejeon, Republic of Korea) | Lee, K.Y. (Chungnam National University, Daejeon, Republic of Korea) | Park, O.S. (Chungnam National University, Daejeon, Republic of Korea) | Kim, S.H. (Chungnam National University, Daejeon, Republic of Korea) | Yook, H.S. (Chungnam National University, Daejeon, Republic of Korea) | Kim, M.K. (Chungnam National University, Daejeon, Republic of Korea), E-mail: [email protected]
In order to study about the lobule and layer formation and cell migration of the mouse cerebellum from at the birth to 15th day effected by 2.5, 5 and 10 Gy r-raddiation at the 19th pregnancy. The routine tissue preparation and staining procedure, Immunohistochemical staining method by the several antibody and western brotting method were utilized from the birth to the 15th day. The results were as followings. 1. The body and cerebellum weights were more slowly increase of the the 2.5 Gy, 5 Gy and 10 Gy irradiation group compare to the control group, and the health condition of the 2.5 Gy group was a little bad. but the 10 Gy group was more severe and begun to die from the 12th day after birth.
Show more [+] Less [-]Proteome analysis of chickens fed with tissue culture medium waste after harvest of Korean wild ginseng
2005
Seol, J.W. (Chonbuk National University, Jeonju, Republic of Korea) | Hwang, I.H. (National Livestock Research Institute, Cheonan, Republic of Korea) | Kang, C.S. (EnT Co. Ltd., Nonsan, Republic of Korea) | Chae, J.S. (Chonbuk National University, Jeonju, Republic of Korea) | Kang, H.S. (Chonbuk National University, Jeonju, Republic of Korea) | Ryu, K.S. (Chonbuk National University, Jeonju, Republic of Korea) | Park, S.Y. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: [email protected]
Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in chickens fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n=60/group) of day old broiler chickens were administered with 0 (control) and 0.8% (treatment) TCM-KWG through drinking water. After 5 weeks, we examined the protein expression pattern of fibularis longus and superficial pectoral muscle by Two-dimensional electrophoresis analysis. Interestingly, TCM-KWG treatment significantly increased five spot's density, and markedly reduced five spot's density in the muscles.
Show more [+] Less [-]Antibiotic resistance pattern of Enterococcus spp. and Staphylococcus aureus isolated from chicken feces
2005
Lee, Y.J. (Kyungpook National University, Daegu, Republic of Korea), E-mail: [email protected] | Kim, A.R. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Jung, S.C. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Song, S.W. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Kim, J.H. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea)
This study was carried out to investigate the antibiotic resistance pattern of Enterococcus spp. and Staphylococcus aureus (S. aureus) isolated from chicken feces. All isolates showed high resistance to erythromycin (E) and tetracycline (TE). Of the 63 Enterococcus faecalis (E. faecalis) isolates, 73.0% were resistant to E and 98.4% to TE. Of the 44 Enterococcus faecium (E. faecium) isolates, 50.0% were resistant to E and 95.5% to TE. Of the 52 S. aureus isolates, 57.6% were resistant to E and 96.2% to TE. The prevalence of two and three drugs resistance pattern were 28.6% and 17.5% of E. faecalis, 40.9% and 25.0% of E. faecium and 38.5% and 23.1% of S. aureus, respectively.
Show more [+] Less [-]Development of the rapid detection kit for Salmonella spp. using immunochromatographic assay
2005
Jung, B.Y. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea), E-mail: [email protected] | Jung, S.C. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea)
An immunochromatographic (IC) strip for the rapid detection of Salmonella spp. in the enriched sample was developed. Affinity purified Salmonella polyclonal antibody was conjugated with 40 nm colloidal gold particles which were prepared by citrate method in our laboratory. The antigen-antibody-gold complex was captured by Salmonella antibody attached to test line of nitrocellulose membrane during the capillary migration of sample. Specificity of the IC strip was calculated to be 100% (12/12) and sensitivity was 97.6% (41/42) in the test with pure cultured bacteria. Salmonella was artificially inoculated into raw pork macerated with enrichment broth.
Show more [+] Less [-]Comparison of immune responses of dogs and guinea pigs inoculated with inactivated canine coronavirus vaccines
2005
An, D.J. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Kim, B.H. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea), E-mail: [email protected] | Jung, B.Y. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Yi, C.H. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Jeon, W.J. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Lee, P.S. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Chung, G.S. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea)
Canine coronavirus (CCV) causes a mild gastroenteritis in dogs. The virus is highly contagious. Although the virus was isolated more than thirty years ago, canine coronavirus infection continues to be a widespread problem. Mixed infections with both CCV and canine parvovirus (CPV) are common. Four kinds of commercial killed CCV vaccines are available in Korea. All the commercial vaccines should pass the National Assay for Veterinary Biologicals prior to release. For the potency test of CCV vaccine, it is necessary to use CCV antibody free dogs. The test requires not only kennels but high cost. To develop easy, efficient and economic potency test method for killed CCV vaccine using laboratory animals, a series of experiments with rabbits and guinea pigs were carried out in this study.
Show more [+] Less [-]Renal failure associated with ingestion of grapes and raisins in dogs
2005
Yuk, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, C.H. (Gyeongsangnam-do Livestock Promotion Institute, Jinju, Republic of Korea) | Kim, T.Y. (Ministry of Agriculture and Forestry, Kwacheon, Republic of Korea) | Kang, C.B. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
This experiments was designed to observe the effect of period and dose of ingestion of grapes on renal failure in dog. After the dogs had been mixed of general feed, raisins from U.S.A and fresh grapes from Chile selling in Korea. We observed the clinical sign, and analyzed the blood and urine using by biochemical test. The experiment was executed by the two following groups. The experiment group 1. derived renal failure by supplying the mixed general feed with fresh grapes and dry grapes 15 g per weight kg, 2 times a day (AM 09, PM 21), the experiment group 2. executed to derive renal failure by supplying the mixed general feed with fresh grapes and dry grapes 40 g per weight kg, 2 times a day.
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