Objectives: The objective of this study was to evaluate the impact of the antimicrobials nisin and lysozyme to control the growth of spoilage bacteria of pasteurized milk during cold storage. Materials and Methods: Nisin, lysozyme, and a mixture of them were inoculated into freshly pasteurized milk at 500 IU/ml concentrations each. The acidity, sensory evaluation, and bacteri¬ological quality of the treated pasteurized milk samples were examined at zero time and every 3 days till the samples showed the signs of spoilage, that were checked every day. Results: Obtained results showed that there was a slight increase of the titratable acidity of the control and treated samples during refrigerated storage, but the acidity increase was significantly lower in samples containing lysosomes and/or nisin than the control samples. Nisin and lyso¬zyme at 500 IU/ml concentration possessed inhibitory effect on the total bacterial, aerobic spore-formers, and psychrotrophic bacterial counts and extended the shelf-life of the treated samples. The efficacy of nisin 500 IU/ml combined with lysozyme 500 U/ml was assessed and synergistic activity has been detected, that was expressed in the form of higher inhibitory effect and extend¬ing the shelf-life of the samples up to 15 days at cold storage. Moreover, the sensory evaluation showed that nisin and lysozyme does not affect the acceptability of the examined samples. Conclusion: The obtained data indicate that nisin and lysozyme have the potential to enhance the post-process bacteriological safety of pasteurized milk during the storage period and could aid in the elimination of post-process contamination and prolong its shelf-life. [J Adv Vet Anim Res 2019; 6(3.000): 403-408]

Objectives: The objective of this study was to evaluate the impact of the antimicrobials nisin and lysozyme to control the growth of spoilage bacteria of pasteurized milk during cold storage. Materials and Methods: Nisin, lysozyme, and a mixture of them were inoculated into freshly pasteurized milk at 500 IU/ml concentrations each. The acidity, sensory evaluation, and bacteri­ological quality of the treated pasteurized milk samples were examined at zero time and every 3 days till the samples showed the signs of spoilage, that were checked every day. Results: Obtained results showed that there was a slight increase of the titratable acidity of the control and treated samples during refrigerated storage, but the acidity increase was significantly lower in samples containing lysosomes and/or nisin than the control samples. Nisin and lyso­zyme at 500 IU/ml concentration possessed inhibitory effect on the total bacterial, aerobic spore-formers, and psychrotrophic bacterial counts and extended the shelf-life of the treated samples. The efficacy of nisin 500 IU/ml combined with lysozyme 500 U/ml was assessed and synergistic activity has been detected, that was expressed in the form of higher inhibitory effect and extend­ing the shelf-life of the samples up to 15 days at cold storage. Moreover, the sensory evaluation showed that nisin and lysozyme does not affect the acceptability of the examined samples. Conclusion: The obtained data indicate that nisin and lysozyme have the potential to enhance the post-process bacteriological safety of pasteurized milk during the storage period and could aid in the elimination of post-process contamination and prolong its shelf-life. J. Adv. Vet. Anim. Res., 6(3): 403-408, September 2019

Objective: The aim of this study is to determine the effect of laser diode as an alternative treat¬ment on liver dysfunction (in vivo study) that is caused by carbofuran using male mice (Mus musculus) strain Balb/C. Materials and Methods: The samples were divided into three groups, namely, Group CL (con¬trol group, no treatment), Group C+L (only treated by carbofuran treatment), and Group C+L+ (treatment group, treated by carbofuran and laser-puncture) with five replications each. After being treated, each liver slice of samples was observed using microscope to get the histology result and then scored. Results: Carbofuran contamination can lead to inflammation of cells and necrosis. The histology results and the scoring test showed that the liver cells repair with the energy dose of laser diode at 0.5 and 1.0 Joule. Conclusion: The optimum energy dose in this study was 1.0 Joule which had the closest score of inflammatory cells and necrosis to normal liver cells. [J Adv Vet Anim Res 2019; 6(4.000): 499-505]

Objective: The aim of this study is to determine the effect of laser diode as an alternative treat­ment on liver dysfunction (in vivo study) that is caused by carbofuran using male mice (Mus musculus) strain Balb/C. Materials and Methods: The samples were divided into three groups, namely, Group C–L– (con­trol group, no treatment), Group C+L– (only treated by carbofuran treatment), and Group C+L+ (treatment group, treated by carbofuran and laser-puncture) with five replications each. After being treated, each liver slice of samples was observed using microscope to get the histology result and then scored. Results: Carbofuran contamination can lead to inflammation of cells and necrosis. The histology results and the scoring test showed that the liver cells repair with the energy dose of laser diode at 0.5 and 1.0 Joule. Conclusion: The optimum energy dose in this study was 1.0 Joule which had the closest score of inflammatory cells and necrosis to normal liver cells. J. Adv. Vet. Anim. Res., 6(4): 499-505, December 2019

Objective: The study was carried out to develop a wet litter model with magnesium chloride to assess the effectiveness of a polyherbal formulation (PHF) on growth performance, litter and cecal moisture (LCM) level, cecal consistency (CC) score, and footpad lesions (FPLs) score in Ross 308 broiler chickens. Materials and Methods: 1,200 one-day-old chicks were assigned into five groups: normal control, negative control [NTC; treated with 1.7% magnesium chloride hexahydrate (MgCl2.6H2O)], and three treatment groups, T1, T2, and T3, where 750, 1,000, and 2,000 gm/ton of PHF, respectively, were supplemented. All the groups were fed a basal diet until day 7. However, the NTC and treat¬ment groups were fed a diet with MgCl2 from days 8 to 42. Results: The addition of MgCl2 for 35 days worsened the growth performance traits in broilers and induced wet litter problems (FPL, high LCM, and poor CC) in the NTC group. However, PHF (750, 1,000, and 2,000 gm/ton) ameliorated the negative effect of a diet with MgCl2 on growth perfor¬mance and wet litter problems, but a better response with respect to LCM and CC was observed in 2,000 gm/ton of PHF group, followed by that in 1,000 gm/ton of PHF group and 750 gm/ton of PHF group on day 42. Conclusion: The wet litter broiler model was developed through excessive feeding of MgCl2, which caused the performance parameters to worsen and the emergence of problems associated with the wet litter. Supplementation with PHF ameliorated these problems and, therefore, it can be used for the management of wet litter in poultry. [J Adv Vet Anim Res 2019; 6(4.000): 536-543]

Objective: The study was carried out to develop a wet litter model with magnesium chloride to assess the effectiveness of a polyherbal formulation (PHF) on growth performance, litter and cecal moisture (LCM) level, cecal consistency (CC) score, and footpad lesions (FPLs) score in Ross 308 broiler chickens. Materials and Methods: 1,200 one-day-old chicks were assigned into five groups: normal control, negative control [NTC; treated with 1.7% magnesium chloride hexahydrate (MgCl2.6H2O)], and three treatment groups, T1, T2, and T3, where 750, 1,000, and 2,000 gm/ton of PHF, respectively, were supplemented. All the groups were fed a basal diet until day 7. However, the NTC and treat­ment groups were fed a diet with MgCl2 from days 8 to 42. Results: The addition of MgCl2 for 35 days worsened the growth performance traits in broilers and induced wet litter problems (FPL, high LCM, and poor CC) in the NTC group. However, PHF (750, 1,000, and 2,000 gm/ton) ameliorated the negative effect of a diet with MgCl2 on growth perfor­mance and wet litter problems, but a better response with respect to LCM and CC was observed in 2,000 gm/ton of PHF group, followed by that in 1,000 gm/ton of PHF group and 750 gm/ton of PHF group on day 42. Conclusion: The wet litter broiler model was developed through excessive feeding of MgCl2, which caused the performance parameters to worsen and the emergence of problems associated with the wet litter. Supplementation with PHF ameliorated these problems and, therefore, it can be used for the management of wet litter in poultry. J. Adv. Vet. Anim. Res., 6(4): 536-543, December 2019

Objective: The morphometric and meristic variations of Xenentodon cancila was studied based on the landmark-based truss network system to assess their phenotypic variations among four different freshwater stocks, viz. Boluhorpur baor, Jhenaidah (BBJ) (n = 29); Bhairab River, Jashore (BRJ) (n = 34); Arial Khan River, Madaripur (AKRM) (n = 28), and Bohnni baor, Gopalganj (BBG) (n = 25) in Bangladesh. Materials and methods: Seven meristic characters were counted by using a needle. Eight morphometrics and 28 truss measurements were measured by using tpsDigV.2.1 software. In meristic characters, KruskalWallis test was performed to determine any significant differences, whereas, in morphometrics and truss measurements, univariate statistics and discriminant function analy-ses were carried out by using SPSS 22 version. Results: Significant differences were observed in four meristic characters among seven meristic characters in the KruskalWallis test. In univariate statistics, only nine characters were observed significantly different among eight morphometrics and 28 truss measurements. The contribution of three discriminant function analyses (DFA), in which first DFA showed 49.2%, second DFA showed 33%, and third DFA showed 17.8% on behalf of both morphometric and truss measurements. In discriminant space, the four stocks were clearly separated. Two clusters were formed among four stocks, where BBG formed a single cluster, whereas BBJ and BRJ aggregately formed another cluster. Additionally, AKRM formed a sub-cluster with BBJ. Conclusion: The preliminary information generated from the current study would be beneficial for further genetic studies and in the assessment of ecological impacts on X. cancila stocks in Bangladesh. [J Adv Vet Anim Res 2019; 6(1.000): 117-124]

Objective: The morphometric and meristic variations of Xenentodon cancila was studied based on the landmark-based truss network system to assess their phenotypic variations among four different freshwater stocks, viz. Boluhorpur baor, Jhenaidah (BBJ) (n = 29); Bhairab River, Jashore (BRJ) (n = 34); Arial Khan River, Madaripur (AKRM) (n = 28), and Bohnni baor, Gopalganj (BBG) (n = 25) in Bangladesh. Materials and methods: Seven meristic characters were counted by using a needle. Eight mor­phometrics and 28 truss measurements were measured by using tpsDigV.2.1 software. In meristic characters, Kruskal–Wallis test was performed to determine any significant differences, whereas, in morphometrics and truss measurements, univariate statistics and discriminant function analy­ses were carried out by using SPSS 22 version. Results: Significant differences were observed in four meristic characters among seven meristic characters in the Kruskal–Wallis test. In univariate statistics, only nine characters were observed significantly different among eight morphometrics and 28 truss measurements. The contribu­tion of three discriminant function analyses (DFA), in which first DFA showed 49.2%, second DFA showed 33%, and third DFA showed 17.8% on behalf of both morphometric and truss measure­ments. In discriminant space, the four stocks were clearly separated. Two clusters were formed among four stocks, where BBG formed a single cluster, whereas BBJ and BRJ aggregately formed another cluster. Additionally, AKRM formed a sub-cluster with BBJ. Conclusion: The preliminary information generated from the current study would be beneficial for further genetic studies and in the assessment of ecological impacts on X. cancila stocks in Bangladesh. J. Adv. Vet. Anim. Res., 6(1): 117-124, March 2019

Objectives: Drug residues in poultry products could lead to the development of antibiotic-resistant bacteria as in any living animal and human alike. Extensive use of antibiotics in animals to promote growth rate, increase feed efficiency, and for prevention of intestinal infections has led to the development of resistant bacteria in the gastrointestinal tract. The present study was conducted to evaluate the effects of biological supplementation of probiotic, phytobiotic, and their combination over antibiotic on growth performance, microbial load, and hematological parameters in Broiler. Materials and methods: Sixty-five broiler chicken were divided into five groups (12 birds in each group), namely, group A (basal diet), group B (antibiotic, Renamycin 100®), group C (phytobiotic, Galibiotic®), group D (probiotic, Bio-Top®), and group E (combination, Galibiotic®+Galibiotic®) and five were sacrificed for baseline data on day 0. Results: Average final live weight gain was highest in group D (probiotic) than other groups. The feed conversion ratio was highest in group A and lowest in the probiotic group (group C). Blood samples were collected on 14th and 28th day for hematological studies. The mean hematology values regarding the total erythrocyte count, hemoglobin concentration, packed cell volume, and erythrocyte sedimentation rate differed significantly (p < 0.05) among groups. The pH of all the treatment groups was significantly decreased compared to the control group (p < 0.05) where group C was significantly (p < 0.05) lower than all other groups. Highest total viable cell count was observed in control (group A) and total coliform count in phytobiotic (group C) was significantly lower than in other treatment groups (p < 0.05). Conclusion: It may conclude that biological supplements have a significant positive impact on growth performance, hematological parameters, and gut microbial load in broiler chicken of which the probiotic showing the best effects. Supplementation of probiotic in feed could be one of the best candidates as an alternative to antibiotics as growth promoter for safe broiler production. [J Adv Vet Anim Res 2019; 6(3.000): 409-415]

Objectives: Drug residues in poultry products could lead to the development of antibiotic-resistant bacteria as in any living animal and human alike. Extensive use of antibiotics in animals to promote growth rate, increase feed efficiency, and for prevention of intestinal infections has led to the development of resistant bacteria in the gastrointestinal tract. The present study was conducted to evaluate the effects of biological supplementation of probiotic, phytobiotic, and their combination over antibiotic on growth performance, microbial load, and hematological parameters in Broiler. Materials and methods: Sixty-five broiler chicken were divided into five groups (12 birds in each group), namely, group A (basal diet), group B (antibiotic, Renamycin 100®), group C (phytobiotic, Galibiotic®), group D (probiotic, Bio-Top®), and group E (combination, Galibiotic®+Galibiotic®) and five were sacrificed for baseline data on day 0. Results: Average final live weight gain was highest in group D (probiotic) than other groups. The feed conversion ratio was highest in group A and lowest in the probiotic group (group C). Blood samples were collected on 14th and 28th day for hematological studies. The mean hematology values regarding the total erythrocyte count, hemoglobin concentration, packed cell volume, and erythrocyte sedimentation rate differed significantly (p < 0.05) among groups. The pH of all the treatment groups was significantly decreased compared to the control group (p < 0.05) where group C was significantly (p < 0.05) lower than all other groups. Highest total viable cell count was observed in control (group A) and total coliform count in phytobiotic (group C) was significantly lower than in other treatment groups (p < 0.05). Conclusion: It may conclude that biological supplements have a significant positive impact on growth performance, hematological parameters, and gut microbial load in broiler chicken of which the probiotic showing the best effects. Supplementation of probiotic in feed could be one of the best candidates as an alternative to antibiotics as growth promoter for safe broiler production. J. Adv. Vet. Anim. Res., 6(3): 409-415, September 2019