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10-DETECTION OF AFLATOXIN M1IN LOCAL CHEESE OF MOSUL CITY, IRAQ Full text
2018
I.A. Sultan | A.M. Shareef | I.I. Khalil | H.S. AL-Naemi
A total number of 90 (45 soft white and 45 processed) local cheese samples purchased from Mosul City supermarkets from March to June 2013 were analyzed for Afla toxine (M1),AFM1 using competitive Enzyme-Linked Immunosorbent Assay (ELISA). Results showed that 37 soft white cheese samples (82.22%) were positive to residual AFM1 with a range between 0.000-0.470 ppb and a mean of 0.133 ppb, while all the 45 processed cheese samples (100%) were contaminated with AFM1 at a range between 0.040-0.810 ppb and a mean of 0.213 ppb the difference between mean was significant (p
Show more [+] Less [-]22- RESIDUAL DISTRIBUTION OF LEAD, CHROMIUM AND COBALT IN DAIRY PRODUCTS AND THEIR BY-PRODUCTS MANUFACTURED FROM MILK SPIKED WITH THESE METALS Full text
2018
Hiba S. Al-Naemi
The research study the distribution patterns and residues behavior of Pb, Cr and Co between dairy products [yoghurt, cheese and fatty dairy products ( kishfa, Gaymer and cream)] and their by-products (whey and skim milk) manufactured from experimentally polluted raw ewe̓ s, buffalo̓ s and cow̓ s milk were investigated using Atomic Absorption Spectrophotometer (AAS). The studied metals (Pb, Cr and Co) were concentrated in both cheese and fatty dairy products (kishfa, gaymer and cream) as they recorded concentration factors in cheese produced from raw ewe̓ s, buffalo̓ s and cow̓ s milk of 3.849, 3.466 and 2.452, 3.804, 2.938 and 2.342 and 2.995, 2.682 and 2.336 folds, respectively and 3.693, 3.164 and 2.339, 3.347, 2.773 and 2.242 and 2.601, 2.156 and 2.14 folds, respectively in kishfa, gaymer and cream produced from raw ewe̓ s, buffalo̓ s and cow̓ s milk compared with metals concentrations reported in raw milk used for preparing these products, whereas the by-products of cheese and fatty dairy products processing (whey and skim milk, respectively) contained Pb, Cr and Co concentrations lower than these present in raw milk as they recorded reduction levels of 71.6, 58 and 31.7, 63.7, 39.5 and 27 and 31.4, 20 and 9.5%, respectively in whey and 37.3, 29.9 and 12.1, 41.7, 27 and 19.3 and 21.3, 7.8 and 5.2%, respectively in skim milk. Yoghurt processed from the raw ewe̓ s, buffalo̓ s and cow̓ s milk contained Pb, Cr and Co metals at mean concentration levels similar to and slightly less than those found in raw milk as the reduction levels amounted 0, 0.6 and 1, 0.1, 0.7 and 1.4 and 0.6, 0.8 and 1.9%, respectively. Generally, metals exhibited significant differences (p˂0.05) in their distribution and residues behavior in dairy products and their by-products and comparable to raw milk used in processing as they arranged as follows: cheese> fatty dairy products (kishfa, Gaymer and cream)> raw milk> yoghurt> skim milk> whey, except yoghurt and raw milk which difference was not significant (p˂0.05). Also, metals found in studying dairy products in the order of Pb > Cr > Co, in contrast to their by-products as they arranged reversibly. Finally, metals concentrated in cheese and kishfa produced from raw ewe̓ s milk, in addition to cheese and gaymer produced from raw buffalo̓ s milk at levels significantly higher (p˂0.05) than those present in cheese and cream produced from raw cow̓ s milk.
Show more [+] Less [-]20- STUDY THE EFFECT OF BAY LEAF EXTRACTON THE SOMEBIOCHEMICAL PARAMETERS IN DIABETIC MALE RAT INDUCED BY ALLOXAN Full text
2018
Adel M. Hassen Alzobidy | Jian Ibraheem k
The present study aimed to determine the ameliorative effect of bay leaf extract(BLE) on some biochemical parameters of laboratory diabetic male rats (Rattusnorvegicas) induced by Alloxan .The study consisted of thirty adult male rats randomly divided into five equal group(six of each ). As follow :Group one the animals treated by intra peritoneal injection (IP) of normal saline solution (0.25ml),Group two the animal treated by oral gavage with (BLE) at dose (500 mg /Kg/BW) daily .Group three , rats were treated I.P with 100 mg /Kg BW single dose of alloxan(Allox).Group four .The diabetic rats were given orally by oral gavage low dose of ( BLE)(250 mg /Kg/BW ) daily. Group five ,diabetic rats were given by oral gavage high dose of BLE(500 mg /Kg/BW ) daily for 3 weeks.At the end of experiments period ,rats were sacrificed ,blood were collected by cardiac puncture to investigate biochemical parameters which included glucose ,liver enzyme (ALT,AST and ALP ) lipid profile TG , TC ,HDL-c , LDL-c , ,Total serum protein, Blood urea, and Creatinine concentration . Result indicated a significant increased in Glucose,ALT, AST, ALP, TC, TG, LDL-c ,urea, and Creatinineinin diabetic animal whereas HDL-c significantly decreased
Show more [+] Less [-]14- STUDY EFFECT ELECTROMAGNETIC FIELD (EMF) AND MOBILE PHONE RADIATION ON SOME HEMATOLOGICAL, BIOCHEMICAL AND HORMONAL PARAMETERS IN FEMALE RATS Full text
2018
Rashad F. Ghadhban | Azhar A. Mhaibes
This study was conducted to investigate the effect of Electromagnetic field (EMF) and mobile phone radiation on some hematological, biochemical and hormonal levels. This study was achieved on 36 mature female rat, divided in to three groups each one consist of 12 animal , first group was control groups without exposure to any effects. Second group was exposed to electromagnetic field(50- 60 Hz) for 30 minutes daily for 45 days, third group was exposed to Mobile phone radiation for 30 minutes and the period of exposed was daily for 45 days. Blood samples were collected from treated and control rats about 6ml divided in to two tubes one with anticoagulation for complete blood test and other without anticoagulation for hormonal and biochemical analysis. Results revealed that there were significant( p≤0.05) decrease in RBC count when exposed to electromagnetic field and mobile radiation while that there were significant( p≤0.05) increase in WBC when were exposed to electromagnetic field. While there were significant( p≤0.05) decrease in WBC when female rats were exposure to mobile phone radiation. There were significant (p≤0.05) increase in cholesterol and triglyceride when exposed to electromagnetic field and mobile phone radiation, there was significant (p≤0.05 ) decrease in FSH level in mobile phone radiation group in compared with control and156 magnetic groups. Significant (p≤0.05) decreased in LH level of mobile groups compared with control groups. INTRODUCTI
Show more [+] Less [-]THE INFLUENCE ON COMMUNITY-HEALTH AND FOODHYGIENE VIA ISOLATED SALMOMELLA FROM RAW-FOODS AND MORE-RECENT ANTIBIOTIC SENSITIVITY PATTERNS, TAIF, KSA Full text
2018
Sherifa Mostafa M. Sabra
This work aimed to confirm "The influence Salmonella on community-health (CH) and food-hygiene (FH) in raw-foods (RFs) and More-recent (MR) antibiotic sensitivity patterns to Salmonella. In Taif, (KSA), the results was (35.0, 25.0, 17.5, 10.0, 7.5 and 5.0%) for Salmonella types (Non typhi group D, Para typhi group B, Para typhi group A, Para typhi group C, Salmonella Spp and Enteritidis), respectively. The effect of antibiotics were (CAX, CFT, C, CP, T/S and AM) as (98.8, 97.7, 87.3, 85.3, 65.7 and 48.5%) respectively. The scores were (100, 89.5, 84.0, 83.3, 76.5 and 66.7%) for Salmonella (Spp., Non typhi group D, Para typhi group B, Enteritidis, Paratyphi group A and Paratyphi group C), respectively. The excellent MR antibiotics were (CAX, CFT, C, CP and T/S), the effect ratios were given 100%. The good MR antibiotics were (C, CP, CAX, T/S, CFT and AM), the effect ratios were given (75-100%). The approved MR antibiotics were (Am, T/S, C and CP), the effect ratios were given (50-75%). The not approved MR antibiotics were (AM, T/S and AM), the effect ratios were given (under 50%). That concluded must take the hygienic precautions and healthy measures such as non-use of antibiotics in animal and bird rations, monitoring of (slaughter-houses, grocery, stores, fast food shops and their employees), making "Rapid Treatment System" for infected individuals is recommended. The "Protective Medicine Unit" and "Municipals Unit" belong to MOH must be immediately follow the food bacterial contamination sources for healthy eradication that to protect the CH and FH
Show more [+] Less [-]OVERDOSEOF TUSSIRUM INDUCED HISTOPATHOLOGICAL CHANGES OF THE LIVER AND KIDNEY IN MALE DOMASTIC RABBITS Full text
2018
Zenab M. Abd Al Rahmman | Yasmeen J. Mohammed | Abdal J. Rasmy | Majdy F. Majeed
In this study, the histopathological changes due to overdose usage of Tussirum drug(0.75 and 3 ml/Kg)in liver and kidney were assessed in rabbite with light microscopes. Six male rabbites(1 ± 0.5 Kg ) were included and divided into three groups.Normal saline (3ml/Kg) wasgiven orally as placebo in the control groupI (N= 2). Group II and III(N= 4 for each) was received Tussirum orallyat a single dose of (0.75 and 3ml/kg/day) for 30 days respectively. the results were recorded that both doses of Tossirum were induced that blood vessel congestion, aggregation of Kuppfer cells,inflamintion infiltrations and Sinusoidal dilatation in the liver parenchyma in addition to the cytoplasmic vacuolation, degeneration, pyknotic nuclei in the hepatocytes and. On the other hand, renal damage was observed in the kidneys of treated rabbits, necrosis of glomular, degeneration of nucli and degeneration in the lining epithelial cells of renal, also noted that glomular shrinkage, dilated of reanal tubules and hyperplasia of tubles walls,as well as to necrosis of renal tubules endothelium, closing of renal tubules lumen, isulation of renal tubules endothelium and density in some nucli chromatic Finally, the investigators concluded that Tossirum toxicity induced hepatocellular and renal damage
Show more [+] Less [-]11-EFFECT OF CINNAMON ZEYLANICUM BARK WATER EXTRACT ON MALE DIABETIC ALBINO RATS FERTILITY Full text
2018
Ahlam J.H.AL-Khamas
The effects of Cinnamon zeylanicum (CZ) bark extracts on productivity of male diabetic albino rats testosterone stages were studied. The experimentation was carried out on 24 male albino rats allocated into 4 equivalent groups of 6 animals each. One group was served as a normal control, whereas rats of the groups (2) were given Alloxan (120 mg.B.W / day for 3 days) by intraperitoneal injection for induction of diabetes was leftward as a diabetic control, while rats of groups (3) CZ extract treated by 500 mg.kg B.W.orally, while rats of groups (4) CZ extract treated 500 mg. BW orally,for 6 weeks without alloxan treatment. Results showed that oral administration of CZ extracts to diabetic rats for 6 weeks significant increased serum testosterone levels. The dose of extracts ameliorated the degenerative lesions which found in diabetic rats testes, This study recommend that drinking of CZ bark as a extract may be beneficial for diabetic patients who suffer from sexual impotency as it improve fertility and lessen the high blood glucose level in male diabetic rats.
Show more [+] Less [-]STUDY OF BIOFLOC PRODUCTION FROM FISH BREEDING SYSTEM AND USING AS SUPPLEMENT IN RUMINANT DIET Full text
2018
Asad Pormohammad | Tahereh Mohammadabadi | Morteza Chaji | Khalil Mirzadeh | Jasem G. Marammazi
The aim of this work was to produce biofloc from the fish breeding system and study of its effect on in vitro ruminal digestibility and gas production in the ruminants. In the first phase of the experiment, two hundred common carp fishes (1gr) in two tanks with capacity 250 liters’ water were used for producing biofloc. In the second stage, the effect of 0, 0.5, 1, 1.5 and 2% of produced biofloc with ration 70 to 30 concentrate to forage on in vitro gas production and digestibility was determined. The data were analyzed in a completely randomized design (5 treatments and 4 replicates). The results showed that there was no significant difference in potential and rate of gas production and dry matter digestibility between treatments. The highest amount of truly digested organic matter, cell wall degradability, and NDF digestibility was related to the treatment containing 2% compared with the other treatments (108.4, 67.8, and 32.5, respectively). The10 microbial biomass efficiency, microbial biomass and PF of treatments containing biofloc were more than control treatment (P < 0.05). On the base of this trial, treatments containing biofloc especially the high levels of biofloc had a more positive effect on digestibility and fermentation of experimental diets, therefore biofloc produced from the fish breeding system can be used as a useful supplement in ruminants.
Show more [+] Less [-]25- BACTERIOLOGICAL ISOLATION AND EVALUATION OF THE ANTIMICROBIAL RESISTANCE OF ENTEROBACTERIAL ISOLATES FROM GALL BLADDER OF SLAUGHTERED CATTLE Full text
2018
Ashwaq Raheem Nazzal
The increasing prevalence of antimicrobial resistance in meat-producing animals, especially ruminants, represents a major problem for human and animal and also could increase the patient's morbidity and mortality. The gallbladder may be a sit of persistence and a source for fecal shedding of certain enteric food-borne pathogen resistant to many antimicrobial agents. In the current study 80 samples (32 bile, 48 epithelium) were examined to isolate the enteric pathogen;AII samples were cultured on primary and selective. The frequency of isolation of microorganisms was (60% )in the epithelium and (40% ) in the bile. The major pathogen isolated were (68.57%) Proteus spp. (21.25%)E-coli ,(7.5)Citrobacter ,(1.25) Psudomnas. and(1.25)Klebsiella.The antibiotic resistance was determined by Kirby-bauer disc diffusion method using 10 of routine and practical antibiotics. In antimicrobial testing from both bile and gallbladder epithelium showed sensitivity to the following antimicrobial :amikacin, cefoxitin, chloramphenicol, gentamycin, kanamycin and ciprofloxacin. In conclusion , the current study provide helpful insights into the prevalence of food source pathogens. High level of antibiotic resistance in proteus spp and Ecoli that could transmit to humans through meat and meat products need for monitoring system on the incidence and antimicrobial susceptibility of enteric pathogens in meat animals in slaughterhouses.
Show more [+] Less [-]21- STUDY THE EFFECT OF CAFFEINE ON BODY WEIGHT GAIN AND LIPID PROFILE IN ADULT MALE RATS TREATED WITH HYDROGEN PEROXIDE. Full text
2018
Adel M. Hassen Alzobidy .Abdul Razzak N. Khudair | Faraj H.Joni AL-Bidhani
The present study was aimed to determine the ameliorative effect of caffeine on Body weight and lipid profile in male rats treated with hydrogen peroxide (H2O2) . Seventy Two adult male rats were used in this study . The study included two experiments ,in each experiments 36 males were randomly assigned two six equal groups of six animals in each group .The animals in both experiments were treated with the same substances and doses for each group as follows .Group one (control) animals were drenched normal saline ,Group two animals were treated (5.63 mg/kg. Bw )H2O2 daily by oral gavage also group three , animals were treated with low dose caffeine (150 mg /kg Bw) daily .Group four , animals were treated with high dose caffeine (250 mg /kg Bw) daily . Group five . animals were treated (5.63 mg/kg. Bw )H2O2 dose after 1 h animals were given low dose of caffeine (150 mg/kg Bw) .Group Six animal were treated with H2O2 dose(5.63 mg/kg Bw) each rat after one hour was given high dose of caffeine (250 mg /Kg Bw) . The first experiments lasted for one month and second experiments lasted for two months . At the end of the two experiments, animals of all group were sacrificed under chloroform anesthesia .Blood samples were collected from the heart directly by cardiac puncture and the serum was separated to measure the lipid profile . The result revealed a significant decreased in body weight gain in H2O2in first and second experiments compared with control group . While a significant improvements were recorded in body weight gain in all treated groups compared with H2O2 group but still significantly lower compared with those of control group. A significant increase in TC ,TG and LDL-c were recorded in H2O2 group in both experiments compared with control group on the other hand no significant difference was recorded in HDL-c level in H2O2group in first experiments while a significant decreased was recorded in second experiments compared with control group . Finally a significant degrees of improvement were observed in lipid profile in all treated group compared with H2O2 .
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