Animal production can be more efficient and also sustainable if we reduce CH4 production from ruminal fermentation. One option is to find alternative forages that modify rumen fermentation. CH4 is not only harmful the environment but also means loss to the animals. All of the aspects of the issue is related to the condition of ruminant’s farm in Indonesia. Some other forages that are mainly fed as protein source to ruminants, are: cassava leaves, sweet potato leaves, soya bean leaves, tofu waste, leaves of Artocarpus heterophyllus, Musa paradisiaca L, Ipomea batatas. Roughage sources are hays of Panicum maximum, Pennisetum purpureum, and Setaria sphacelata and the concentrate sources mainly corn, rice bran and cassava waste, and corn cobs. However, there are very limited studies in finding alternative forages that can both increase animals productivity and also reduce CH4 production. Only forages relevant to Indonesia that have been studied in vitro is reviewed in this article, about its potential in reducing CH4 production from rumen fermentation. Even though some forages reduce CH4, it could negatively influence digestibility, hence less productivity. Some studies indicated that it was due to the fat content of the forage while others indicated that the concentration of the bioactive compounds such as condensed tannin influence the side effect of low CH4 ruminal production.

The study was conducted in the Tinggi Raja village and Sumber Harapan Village of Sub district Tinggi Raja, in the Asahan District. Each location study has the same agro-ecosystems. The study was conducted using survey method in year of 2013 by interviewing 15 farmers respondent as cooperators and 15 non-cooperators. Primary data and secondary data were analyzed economically, using descriptive and quantitative tabulation. The purpose of this study was to determine the increase in business of goat breeders for economic analysis. The results showed that the net profit of the business of goats in cooperators breeders at the amount of Rp.8.411.168,83/year, B/C ratio of 1.4. Then for the non-cooperator farmers at the amount of Rp.1.644.051.24/year, B/C ratio of 1.2. This data showed that the benefit of farmer cooperators is higher than the non cooperator breeders. Therefore we need to implement the introduction of technological innovations for the farmer in non-cooperators breeders. For cooperators they could get into commercial business, that resulted in their goats achieving a higher market value in terms of price.

This study was done to investigate feedback of visitors on the raise of animals as conservation effort in Taman Rusa Park located at Lamtanjong Village of Aceh Besar, Indonesia. This qualitative research was performed from December 2013 up to March 2014. Respondents were 100 visitors visited the park during the study. Visitors’ responses were recorded using structured, closed questionnaire and analyzed using Chi-square. The results showed that visitors had given positive responses on the maintenance of animals in the Taman Park.

The purpose of this study was to characterize the bacterial 16S rRNA gene of Sumatran orangutan (Pongo abelii) Bukittinggi West Sumatera zoo. The sample used in this study are lactic acid bacteria of the Sumatran orangutan (Pongo abelii) derived from zoo Bukittinggi West Sumatra Indonesia. This study was an exploratory study that conducted at the Laboratory through several stages. The first stage was the isolation of LAB from faeces of Sumatran orangutans using MRS agar medium and then cultured in a liquid medium NB. The next stage was the isolation of total DNA, and then, the third stage was the amplification of the 16S rRNA gene and agarose gel electrophoresis. Then,in the fourth stage of determining the DNA sequence and analysis of DNA sequence homology. The final stage was the computational analysis of 16S rRNA gene Sumatran orangutan (Pongo abelii). The results showed that lactic acid bacteria (LAB) from the Sumatran orangutan (Pongo abelii) is close to lactic acid bacteria Lactobacillus helveticus strain IMAU50151 with the levelof similarity of 89%. It is possible that these bacteria is a new species or the species that LAB has not been reported in Genbank.

The objective of this study was to determine the effect of centella leaf extract administration on decreased of the molecule cAMP responsive element modulator (CREM) expression in the testicular seminiferous tubules of male rats (Rattus norvegicus). Eight rats, aged 3.5 months with 150-250 grams of body weight (BW) were used in this study. All rats were divided randomly into four groups as if K0 as a control group whereas K1, K2, and K3 were given the centella leaf extract with doses 125, 250, and 500 mg / kg body weight respectivelly that given once daily for 30 days. At the end of the treatment, rats were sacrificed and their testes were collected and subsequently fixed in buffered neutral formalin (BNF) 10% as fixative solution for histological preparation. The CREMs expressions were detected using immunohistochemical methods. The results showed that the number of CREM expression in the seminiferous tubules significantly differ (P 0.05) between K0 and the treatment group (K1, K2, and K3). Conclusion, the administration of centella leaf extract with of the dose 125, 250, and 500 mg/kg BW can decreased CREM expression spermatids of testicular seminiferous tubules in male rat.

The cultivation of mulberry as forages is scarce in Indonesian feeding system for ruminant production, although many studies have indicated  its nutritional potential as animal feed especially ruminants. This study was aimed to investigate the effects spacing of plant and cutting age on productivity and chemical composition of some species mulberry plants. The experiment was conducted in a randomized block design with factorial treatments. The factors species were (M. indica cv kanva, M. nigra, M. cathyana, and M. Multicaulis), the spacing of plants (1 x 0.5 m, 1 x 1 m and 1 x 1.5 m) and cutting ages (210, 240, and 270 days after planting). The results showed that species, M. multicaulis and M. cathyana are the highest growth, using a spacing of 1 x 0.5 m with cutting age 270 days after planting. Dry matter production highest (P 0,05) in M. Cathyana at spacing of 1 x 0.5 with cutting age 270 days after planting. The ratio of leaves and stems of plants are affected by age, the highest proportions of leaf  is obtained at the cutting age of 210 days. Chemical composition were highest in M.multicaulis at the age of 270 days. The highest content of  organic matter (OM) were found in M.Kanva and not different with M. Nigra at the age of 270 day after planting. In treatment of cutting ages have no effect (P 0.05) on the Crude protein (CP), ADF, NDF, lignin and tannin content. The average content of CP obtained gained 20.69%, 32.38% ADF, NDF 33.99%, 12.45% lignin and tannin 0.3%. Concluded that mulberry plants with a plant spacing of plant 1 x 0.5 and 270 days after plant have more advantages for use as animal feed.

South American opossums are the definitive hosts of Sarcocystis neurona, Sarcocystis falcatula, Sarcocystis speeri and Sarcocystis lindsayi. The sporocysts of these species of Sarcocystis are morphologically similar and methods like infectivity and pathogenicity for intermediate hosts (immunodeficient mice and psittacine birds) and molecular tools are used for identification. Opossums are synanthropic wild animals, and widely distributed in Brazilian territory. Previous studies have shown high environmental contamination with S. neurona sporocysts in several Brazilian regions. This paper reviews information on Sarcocystis spp. shed by various opossum species and its occurrence in Brazil.

Aeromonas hydrophila and Campylobacter jejuni are bacteria of emerging importance in public health. However, little has been published about fish contaminated by these pathogens. The present study aimed to verify the presence of Aeromonas hydrophila and Campylobacter jejuni in fresh tuna samples (Thunnus spp.) caught off the coast of Santa Catarina State and distributed in the wholesale market of São Paulo/SP. A total of 85 tuna fillet samples were collected and examined by PCR and bacteriological analyses. Aeromonas spp. was detected in 11/85 (13%) samples, with 10/11 (90.9 %) being confirmed as Aeromonas hydrophila by PCR. Campylobacter spp. was found in 10/85 (11.7%) samples, 10/10 (100%) identified as Campylobacter jejuni by PCR and conventional biochemical analyses. Both pathogens were found in 2/85 (2.3%) samples. This is the first report on the contamination of fresh tuna by Campylobacter jejuni and Aeromonas hydrophila in Brazil. In addition to show that tuna can be a vehicle for transmission of pathogens when consumed raw, it emphasizes the importance of further studies to support the control these pathogens in fish.

Foi investigada a infecção de Perkinsus beihaiensis (Perkinsozoa) na ostra Crassostrea rhizophorae em um sistema de cultivo do tipo espinhel e em um estoque natural de ostras no manguezal adjacente ambos localizados no estado da Bahia, Nordeste do Brasil. As colheitas foram realizadas em outubro e novembro de 2012 e em janeiro de 2013. As ostras (n = 300) foram medidas, examinadas macroscopicamente quanto a sinais da infecção e submetidas às técnicas laboratoriais: histologia, ensaio em meio de cultivo de tioglicolato de Ray (RFTM), reação em cadeia da polimerase (PCR) e sequenciamento, que confirmou a identificação do patógeno. As análises histológicas e o RFTM mostraram, respectivamente, prevalência média de 93,3% e de 69%. A infecção foi geralmente leve ou muito leve. Não houve diferença significativa (p > 0,05) entre os ambientes em termos de prevalência ou severidade da infecção. Este é o primeiro registro de P. beihaiensis no estado da Bahia e o segundo em ostras do Brasil e América do Sul.

Previously described in humans and domestic animals, retinal dysplasia has three clinical forms: focal/multifocal, geographic and total. A young orphan crab-eating fox (Cerdocyon thous) from wildlife, male, approximately 45 days old referred to the Wildlife Medicine and Ophthalmology Services of the “Governador Laudo Natel” Veterinary Hospital of the Universidade Estadual Paulista, Jaboticabal Campus, SP, Brazil, where it received primary outpatient care. The patient was in good general health condition, without hematological, biochemistry or serological alterations and no signs of visual impairment. Indirect binocular ophthalmoscopy showed retinal changes in the left eye, distributed over the tapetal area in the form of grayish folds and rosettes. In the affected areas, tapetal reflectivity was reduced. No other ophthalmic abnormalities were observed. This is the first report of retinal dysplasia in the crab-eating fox (Cerdocyon thous) from wildlife.