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Catarrhal mastitis by <i>Staphylococcus simulans</i> in a nulliparous goat Full text
2018
Guilherme Santana de Moura | Michele Flávia Sousa Marques | Fernando Nogueira de Souza | Luciana Bignardi Brisola Casimiro da Costa | Atzel Candido Acosta Abad | Rinaldo Aparecido Mota
The present paper is a case report of a one-year old nulliparous Alpine Goat belonging to a dairy goat farm in semi-arid region of Brazil. Both glands were naturally infected by α-hemolytic Staphylococcus simulans and evolved similar clinical signs. The mammary glands presented an acute catarrhal mastitis with systemic clinical signs that responded positively to treatment with gentamicin associated with amoxicillin. The present report suggests the importance of the pathogenic potential of non-aureus Staphylococci strains (NAS) as a cause of clinical mastitis also in nulliparous animals. The isolate showed resistance to tetracycline and contained staphylococcal toxin production genes (sec, sec and TSST-1). Moreover, it has been reported that Staphylococcus simulans is an emerging pathogen in humans causing cutaneous and osteoarticular infections, mainly in those in close contact with farm animals. To the best of our knowledge, this is the first report of a clinical mastitis in a nulliparous goat caused by Staphylococcus simulans.
Show more [+] Less [-]Successful rehabilitation of Macronectes halli and Macronectes giganteus in southeastern Brazil Full text
2018
Natassia Bacco Mannina | Daniela Magalhães Drummond de Mello | Rebeca Pires Wanderley
This report describes the successful rehabilitation of two species of pelagic seabirds (Macronectes halli and Macronectes giganteus) rescued on the southern coast of the state of São Paulo, Brazil, and taken to the IPeC rehabilitation center (S25°023258 W-47°928737). Data on the enclosures, diet, clinical management and laboratory tests were detailed during the 75 d of treatment, during which the birds remained at the center. This report demonstrates that a combination of environmental and nutritional management with a proper diagnosis and adequate clinical treatment naturally lead to a shorter period in captivity, which is crucial to the successful rehabilitation of these birds. The enclosures should have visual isolation and animals kept separate from other birds. The form of offering food differs based on the feeding habits and taxon. At the time of release back into the wild, the site chosen should be isolated to enable the bird to feel more secure when taking flight. The present report describes the first record of the rehabilitation and release of these two species in Brazil.
Show more [+] Less [-]Subclinical intramammary infection does not affect bovine milk ethanol stability Full text
2018
Cristian Marlon de Magalhães Rodrigues Martins | Juliano Leonel Gonçalves | Bruna Gomes Alves | Marcos André Arcari | Marcos Veiga dos Santos
The present study hypothesized that intramammary infection (IMI) might reduce milk ethanol stability (MES), mainly when IMI is caused by major pathogens. Thus, this study evaluated the effect of IMI on bovine MES using a natural exposure experimental design. Ninety-four lactating cows from five dairy herds were selected once they were determined to have an IMI, based on milk bacteriological culturing with positive isolation and somatic cell count (SCC) > 200×103 cells/mL in two out of three composite milk samples collected during three consecutive weeks. After selection, cows were sampled a second time (within two weeks) for evaluation at mammary quarter level (n = 326): milk yield (kg/quarter/day), MES, composition (fat, protein, lactose, casein, total solids and solids-non-fat), and bacteriologic culture. The effect of subclinical mastitis on MES was tested by two models: 1) comparison of healthy vs. infected quarters; and 2) comparison of contralateral mammary quarter within cow. The only milk composition variable associated with MES was lactose (r = 0.18; P < 0.01). Subclinical IMI did not affect MES when the comparison was performed using both models (1 and 2). Likewise, MES did not change when infected quarters were sorted into two groups of pathogens (major, minor and infrequent; and contagious, environmental, minor and infrequent) and compared with healthy mammary quarters. Considering the results of both models, subclinical IMI did not affect MES of dairy cows.
Show more [+] Less [-]Microscopic sperm head damage and abnormalities as heat stress indicators in Australian Merino rams <i>(Ovis aries)</i> in Northern Patagonia, Argentina Full text
2018
María Fernanda López Armengol | Natalia Rubio | Guillermo Ariel Sabino | Nadia Soledad Bérgamo | Valeria Pelufo
In Northern Patagonia, the mating season starts on March 15th, when rams are submitted to summer temperatures. Exposure of rams to heat stress increases the prevalence of microscopic damage to spermatozoa, morphological abnormalities, and reductions in fertility. This study assesses the adaptive capabilities of six unshorn and six shorn Australian Merino rams, half of which were treated in a heat chamber for eight hours for five days, gradually reaching a temperature of up to 40 °C. Microscopic damage, abnormalities and ultramicroscopic alterations of the plasma membrane and the acrosome of sperm head were analysed. There were significant differences in the percentage of tailless spermatozoa and proximal cytoplasmic droplets between post-treatment periods. Temperature primarily affected the shorn rams and the sperm heads during spermiogenesis. Submicroscopic alterations were observed when the plasma membrane was present in the anterior segment. These alterations can be intact, waved, or dilated. When the plasma membrane was absent, the acrosome might be intact, dilated, and waved. In addition, the outer acrosomal membrane may completely lose its contents or have a nude nucleus. The plasma membrane assumes a waved shape as a result of the effect of temperature on the epididymis. According to this study, the tailless head, proximal cytoplasmic droplets, and the ultramicroscopic categories studied were robust indicators of semen heat stress. After ten weeks, the sperm head recovered its normal shape. Unshorn rams are better adapted to summer heat stress than shorn ones. Microscopy and transmission electron microscopy alterations have been shown to be excellent indicators of thermal stress in Australian Merino rams and may be useful tools to help sheep farmers choose when to begin the mating season, which will vary depending on the environmental conditions of the summer.
Show more [+] Less [-]Bat Coronavirus of Pteropus alecto from Gorontalo Province, Indonesia Full text
2018
Wenty Dwi Febriani | Uus Saepuloh | Ellis Dwi Ayuningsih | R. Suryo Saputra | Azhari Purbatrapsila | Meis Jacinta Nangoy | Tiltje Andretha Ransaleh | Indyah Wahyuni | Safriyanto Dako | Rachmitasari Noviana | Diah Iskandriati | Ligaya ITA Tumbelaka | Joko Pamungkas
Bats have been known as natural reservoirs for potential emerging infectious viruses, such as Lyssaviruses, Coronaviruses, Ebola viruses, Nipah virus, and many others. Because of their abudance in population, wide distribution and mobility, bats have a greater risk as source for zoonotic transmission than other animals. Despite the facts of their role as reservoirs for many pathogens, not until an epidemic of Severe Acute Respiratory Coronavirus (SARS-CoV) in 2003 and Middle-East Respiratory Syndrome Coronavirus (MERS-CoV) in 2012, that people pay much attention about coronavirus in bats. SARS-like virus also found in bats with a higher prevalence rate. This study aims to detect the coronavirus of bats in Gorontalo province Indonesia, characterization at the molecular level of the coronavirus genome and determining the level of kinship (through trees filogenetic). This study was conducted as part of bigger PREDICT Indonesia project, in particular to examine coronavirus in bats from Gorontalo province, Indonesia. As many as 95 rectal swab samples collected from flying foxes (Pteropus alecto) were analyzed in the laboratory using Consensus Polymerase Chain Reaction (PCR) technique to amplify the target sequence from RNA-dependent RNA Polymerase (RdRp) gene with 434 basepair product, resulted 24 samples determined as presumptive positive. Eight out of 24 presumptive positive samples by PCR were analyzed further by nucleotide sequencing and confirmed coronavirus positive. Phylogenetic tree analyses to the eight coronavirus confirmed-sequences were constructed with MEGA-6.0 . The conclusion was 24 out of 95 samples suggested as presumptive positive to Bat CoV. Eight out of 24 samples were analyzed further by nucleotide sequencing and have similarities in the kinship. Three samples had the 98% nucleotide identity to BatCoV from Indonesia and five samples were 85-88% nucleotide identity to BatCoV from Thailand.
Show more [+] Less [-]Association of Infestation of Gastrointestinal Nematode With Altitudes of Location of Cattle Raising in Two Different Districts in Aceh Province Full text
2018
Zulfikar Zulfikar | Sayed Umar | Teuku Reza Ferasyi | Maruf Tafsin
This study was aimed to measure the prevalence and the association of altitude of location of raising to the risk of infestation of gastrointestinal nematode in cattle. The study was conducted using cross sectional approach. A purposive sampling method was used to select the location of study (subdistricts and villages) in the two Districts. The inclusion criteria for the lowland were the location with altitude 200 m above sea level and the subdistricts (villages) of North Aceh District. The exclusion criteria for lowland was the location 200 m above sea level. Then, the inclusion criteria for the highland were the location with altitude 200 m above sea level and the subdistricts (villages) of Central Aceh District. The exclusion criteria for lowland was the location 200 m above sea level. A random sampling was used for selection of cattle for collecting faecal sample in two locations of study. A total number of 201 cattle, both male and female, were obtained for taking faecal sample from the two Districts. Faecal sample was examined for the existence of eggs of gastrointestinal nematode. The examination was conducted using floating method. Data were analyzed descriptively. The measurement of association of altitude of location of cattle raising and sex of animals to the risk of infestation of gastrointestinal nematode were analyzed using odds ratio (OR) estimation and continued with chi-square test. The results showed a higher prevalence of gastrointestinal nematode infestation in cattle of Lowland location (25%) as compared to Highland (5.8%). As for the altitude, the cattle raising in Lowland were more than 5 times higher risk for infestation of gastrointestinal nematode as compared to Higher land location (P0.005). The female cattle in Lowland was higher risk (6.18) for infestation than Highland (P0.01). A similar trend was obtained for male cattle which higher risk in Lowland (4.12) as compared to Highland, but it was not significant (P0.05). In conclusion, by considering the altitude of location, the cattle raising in Lowland were more than 5 times higher risk for infestation of gastrointestinal nematode as compared to Higher land location. There is a variation of risk between female and male cattle in different altitude.
Show more [+] Less [-]Artificial Insemination of Beef Cattle UPSUS SIWAB Program Based on the Calculation of Non-Return Rate, Service Per Conception and Calving Rate In The North Kayong Regency Full text
2018
Duta Setiawan
This study aims to Determine the success rate of the Artificial Insemination in North Kayong Regency of West Kalimantan Province roomates has been done by IB officers. Artificial insemination is the process of introduction or delivery of cement into the genitals of female cows by means of made devices. North Kayong districts Regency is one of the which is administratively established Republic of Indonesia based on Law no. 6 of 2007 is an area that is developing cattle. The location that Became the focus of the evaluation of the success of Artificial Insemination is in three districts of Maya Island district, Sukadana and Seponti. Data used in this research is secondary Data Obtained from inseminator. The parameters used in this evaluation are the Non Return Rate (NRR), Service per Conception (S / C) and Calving Rate (CvR). The results Showed that the average of the three sub-districts in North Kayong district was for the highest 90% NRR in Sukadana district and the Lowest score of 67% in the Mayan Island district. S / C Obtained the highest number of 3.4 in Maya Island district and the Lowest S / C number district of Seponti 1.8. The highest score was 92% CvR in Sukadana district and the cancel CvR score was 64% in the Mayan Island district. The Conclusions Obtained based on the value of NRR, S / C, and CvR on artificial insemination evaluation with the best artificial insemination implementation are Sukadana district, followed by district of Seponti and last is Maya Island district.
Show more [+] Less [-]Effectivity of Entomopathogen of Fungi of Metarhizium brunneum Petch As Biotermitiside for Termite Pest of Coptotermes curvignathus Holmgren on Nutmeg in Aceh Province Full text
2018
Muhammad Sayuthi | Teguh Santoso | Iswadi Iswadi
Abstract
Show more [+] Less [-]Infectious Diseases of Six Non-Domesticated Neo-Tropical Animals In Trinidad and Tobago Full text
2018
Kavita Ranjeeta Lall | Kegan Romelle Jones | Gary Wayne Garcia
This review serves to shed light on some common infectious diseases: their names, prevalence, site of infection and diagnostic tools for identificationof six (6) neo-tropical animals that are found in Trinidad and Tobago with the potential for domestication. These are theagouti(Dasyprocta leporina/ D. agouti), lappe/paca (Cuniculus paca/ Agouti paca), capybara (Hydrochoerus hydrochaeris), manicou/opossum (Didelphis marsupialis insularis), collared peccary (Tayassu tajacu/ Peccari tajucu) and red brocket deer (Mazama americana).There is over Ninety (90) references were used, with the earliest being 1951 and the most recent being in 2017.Where literature was lacking, information from other species within the same genus was incorporated and identified clearly. It was interesting to note that the majority of animals, despite being infected with pathogenic organisms were clinically healthy. In animals that showed clinical signs of disease there was a large quantity of pathogens present. This manuscript sheds some light on the pathogens that may use these animals as natural reservoirs. This review paper also includes many of the earliest identification of these pathogens in Neo-tropical animals in specific geographic regions.
Show more [+] Less [-]Beef Cattle Business in Dry Land Area Parangloe District Gowa Full text
2018
Supardi Rusdiana | Soeharsono Soeharsono | Teuku Reza Ferasyi
The study was conducted in the village Payangkalang Parangloe District of Gowa in South Sulawesi in 2017, with the field survey method Substance himself to Bali beef cattle breeders, through interviews and questionnaires to 18 farmers, by the way (sample random sampling). Respondents beef cattle breeders, classified into 2 (two) business model with a scale of 2 adult males and 3 females scale adult stem, this data will be analyzed using economic analysis of income B/C. Based on the information that the beef cattle population of Bali is quite a many, so that the criterion for consideration for the study area. Beside that all cattle breeders Bali is already a member of a group of farmers. The results showed that net profit in the maintenance of Bali beef cattle female parent of $13.381.250/year with B/C ratio of 1.2 and a gain on the maintenance of male cattle Bali for $ 3.881.250 year, with B/C ratio of 1.1. To maintain a profitable cattle faremr cows, while the majority of beef cattle Bali parent can still be maintained as an investment for the procreation of children next year.
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