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Polychlorinated dibenzo-p-dioxins, dibenzofurans and dioxin-like polychlorinated biphenyls in bivalve molluscs. Risk to Polish consumers?
2023
Mikołajczyk Szczepan | Warenik-Bany Małgorzata | Pajurek Marek
Concentrations of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) were investigated in six species of bivalve mollusc purchased on the Polish retail market. The risk to consumers was calculated as a percentage of the tolerable weekly intake (TWI) (2 pg World Health Organization toxic equivalent (WHO-TEQ) kg−1 body weight (b.w.)).
Show more [+] Less [-]TaqMan real-time PCR for detection of pathogenic Leptospira spp. in canine clinical samples
2023
Rahman Mohammad Sabri Abdul | Khor Kuan Hua | Khairani-Bejo Siti | Lau Seng Fong | Mazlan Mazlina | Roslan Mohd Azri | Ajat Mohd Mokrish Md | Noor Mohd Akmal Mohd
Canine leptospirosis has always been a differential diagnosis in dogs presenting with clinical signs and blood profiles associated with kidney and/or liver disease. The conventional polymerase chain reaction (PCR) provides diagnoses, but real-time PCR-based tests provide earlier confirmation and determine the severity of infection, especially in the acute stage, allowing early detection for immediate treatment decisions. To our knowledge, real-time PCR has not been routinely adopted for clinical investigation in Malaysia. This study evaluated TaqMan real-time PCR (qPCR) assays diagnosing leptospirosis and compared their applicability to clinical samples from dogs with kidney and/or liver disease against a conventional PCR reference.
Show more [+] Less [-]Protein sequence features of H1N1 swine influenza A viruses detected on commercial swine farms in Serbia
2023
Zorić Jelena Maksimović | Veljović Ljubiša | Radosavljević Vladimir | Glišić Dimitrije | Kureljušić Jasna | Maletić Jelena | Savić Božidar
Swine influenza A viruses (swIAVs) are characterised by high mutation rates and zoonotic and pandemic potential. In order to draw conclusions about virulence in swine and pathogenicity to humans, we examined the existence of molecular markers and accessory proteins, cross-reactivity with vaccine strains, and resistance to antiviral drugs in five strains of H1N1 swIAVs.
Show more [+] Less [-]Identification of SNP markers for canine mammary gland tumours in females based on a genome-wide association study – preliminary results
2023
Mucha Anna | Nowak Błażej | Dzimira Stanisław | Liszka Bartłomiej | Zatoń-Dobrowolska Magdalena
The development of genetic research over recent decades has enabled the discovery of new genetic markers, such as single nucleotide polymorphisms (SNPs). This, as well as the full sequencing of the dog genome, has enabled genome-wide association studies (GWAS) to be used in the search for genetic causes of canine mammary tumours (CMTs).
Show more [+] Less [-]Antibody response to 1.0 and 0.5 mL doses of an inactivated bacterial vaccine against bovine respiratory disease in young Holstein calves: a field trial
2023
Mori Kazusa | Kato Toshihide | Kosenda Keigo | Yokota Osamu | Ohtsuka Hiromichi
Early vaccination of cattle with an inactivated commercial bacterial vaccine against bovine respiratory disease has been reported to increase antibody production and can alleviate the disease. However, its dosage has been little investigated in young Holstein calves. This study addresses the need to establish guide values for vaccine dosage in these animals.
Show more [+] Less [-]Inter-laboratory validation of liquid chromatography–tandem mass spectrometry multi-mycotoxin determination in animal feed – method transfer from the reference laboratory to regional laboratories
2023
Jedziniak Piotr | Panasiuk Łukasz | Pietruszka Katarzyna | Kolano Krzysztof | Zbytniewski Radosław
The results are presented of the inter-laboratory validation of a liquid chromatography–tandem mass spectrometry method for the determination of eight mycotoxins (aflatoxin B1, deoxynivalenol, fumonisin B1, fumonisin B2, ochratoxin A, toxin T-2, toxin HT-2 and zearalenone) in animal feeds.
Show more [+] Less [-]Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia
2023
Widodo Agus | Lamid Mirni | Effendi Mustofa Helmi | Tyasningsih Wiwiek | Raharjo Dadik | Khairullah Aswin Rafif | Kurniawan Shendy Canadya | Yustinasari Lita Rakhma | Riwu Katty Hendriana Priscilia | Silaen Otto Sahat Martua
Escherichia coli is an opportunistic bacteria that can grow easily, produce toxins, and resist antibiotics. The phenomenon of E. coli developing multidrug resistance is currently the subject of extensive research. The objective of this study was to molecularly identify blaTEM and blaCTX-M genes in multidrug-resistant E. coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia.
Show more [+] Less [-]Levels of caesium-137 in food of animal origin in Poland
2023
Gembal Magdalena | Czerski Paweł | Milczarczyk Ewelina | Warenik-Bany Małgorzata
Radioactive contamination of the environment is one of the greatest threats after a nuclear accident due to released radionuclides. From a radiotoxicological point of view, the most important radionuclide is caesium-137. Formed mainly during nuclear explosions, caesium-137 can persist in the soil for many years, from where it constantly enters the food chain. One of the elements of ensuring food safety is the monitoring of its radioactive contamination, mainly with radioactive caesium isotopes. The aim of the study was to determine the content of caesium-137 in food of animal origin.
Show more [+] Less [-]Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats
2023
Morgan Ashraf M. | Ogaly Hanan A. | Kamel Shaimaa | Rashad Maha M. | Hassanen Eman I. | Ibrahim Marwa A. | Galal Mona K. | Yassin Aya M. | Dulmani Sharah A. Al | Al-Zahrani Fatimah A.M. | Hussien Ahmed M.
Penconazole (PEN) is a widely applied triazole fungicide. This study sought to define the efficacy of N-acetyl-l-cysteine (NAC) in mitigating PEN-triggered hepatorenal toxicity in rats.
Show more [+] Less [-]Examination of the haematological profile of pregnant Polish Holstein-Friesian black-and-white cattle in the early stage
2023
Wrzecińska Marcjanna | Kowalczyk Alicja | Czerniawska-Piątkowska Ewa | Kordan Władysław | Araujo Jose Pedro
Cattle health and welfare are monitored via the analysis of the haematological profile, and it shows cattle’s ability to adapt to changing environmental conditions, pregnancy and lactation; profile changes also indicate reproductive disorders. The literature lacks reports of the examination of the haematological profile in cows up to the 50th day of pregnancy (dop). Therefore, this research examined that in cows up to this pregnancy stage.
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