peer reviewed | [en] OBJECTIVE: To study the feasibility of ultrasound-guided intervertebral disc (IVD) injection of contrast medium and methylene blue to aid in intraoperative identification of cervical IVD spaces in dogs. METHODS: This was a single-center experimental cadaver study using randomly chosen skeletally mature dog cadavers from January 2, 2023, to March 23, 2023. For each cadaver, 1 cervical IVD was randomly selected. The ventral cervical region was ultrasonographically examined, pushing the trachea leftwards. A 50/50 contrast medium (iohexol, 300 mgI/mL) and methylene blue mixture was injected under ultrasound guidance in the IVD and ventral soft tissues. The x-rays and CT scans were performed before and after injection. Each cadaver was dissected using a ventral slot procedure. The cadavers and IVD characteristics, the success rate in ultrasonographically identifying the correct IVD space, time to injection, most caudal IVD space feasibly injectable, and semiquantitative imaging and surgical scores were recorded. RESULTS: 20 canine cadavers were used. The IVD injections were successfully performed in the correct IVD space in all cadavers. The median time to injection was 3.95 minutes (Q1 to Q3, 3.22 to 5.88 minutes). The contrast medium was clearly visible on at least 1 radiographic projection in all but 1 case and on the CT in all cases. During surgical dissection, the dye was clearly visible in all but 1 case. The dogs' weight was significantly different between most caudal IVD spaces feasibly injectable. CONCLUSIONS: Ultrasonographical cervical IVD space identification and mixture injection are feasible and might help intraoperative cervical IVD space identification in dogs. CLINICAL RELEVANCE: This technique could be used presurgically in patients with cervical IVD diseases.

peer reviewed | A male long-tailed macaque (Macaca fascicularis) was found to have a severe tail injury during a clinical examination prior to vasectomy at the Sacred Monkey Forest Sanctuary in Ubud, Bali, Indonesia. The injury involved an open wound with areas of necrosis and fracture. Based on the tail condition, the animal was treated with a partial caudectomy. Prophylactic treatment prior to surgery with antibiotic and anti-inflammatory was carried out using amoxicillin and ketorolac tromethamine, respectively. Anesthesia was maintained using propofol. Postoperatively, the anesthesia reversal agent atipamezole was administered. After the macaque exhibited clear signs of recovery from anesthesia, it was transported in a cage back to the capture site and released into its original social group. To the best of our knowledge, this is the first documented case report describing partial caudectomy in macaques.

International audience | OBJECTIVE To report clinical signs, biological anomalies, treatment, and outcome in domestic ferrets with cholecystitis, describe the ultrasonographic features of cholecystitis, and compare the ultrasonographic appearance of the hepatobiliary system between diseased and healthy ferrets. METHODS 11 ferrets with confirmed cholecystitis and abdominal ultrasonography were retrospectively included. Ten healthy ferrets were prospectively recruited to undergo hepatobiliary abdominal ultrasonography. Comparisons of explanatory variables (group, age, body weight, and sex) were performed. RESULTS The most common clinical signs were nonspecific, and icterus was observed in 1/4 of diseased ferrets. Compared with healthy individuals, diseased ferrets showed a larger common bile duct (CBD; ≥ 2.8 mm), a thicker CBD wall (≥ 0.7 mm), a thicker gallbladder (GB) wall, more frequent echoic GB luminal content, visible intrahepatic bile ducts, and a greater GB volume. Bile culture was positive in 9/11 mostly for Escherichia coli (8/9). Bile cytology indicated septic purulent cholecystitis in 7/7 ferrets. Survival time after antibiotic treatment ranged from 6 to 104 weeks, with a median of 36 weeks. CONCLUSIONS Ferrets with cholecystitis presented with nonspecific clinical signs and frequently showed hyperbilirubinemia. Ultrasonography findings such as enlarged CBD, thickened CBD wall, thicker GB wall, GB sludge, visible intrahepatic bile ducts, and enlarged GB may suggest cholecystitis. Escherichia coli was commonly isolated from bile. The medium-term mortality rate was high despite targeted antibiotherapy. CLINICAL RELEVANCE This is the first study describing the clinical signs, biological anomalies, ultrasonographic findings, and outcomes after treatment in a series of domestic ferrets with cholecystitis.

The vaccination against bacterial respiratory diseases in calves has been generally recognised as useful for the prevention of infections. Inducing an immunological response after vaccination is important for obtaining protection from infections. The aim of the study was to investigate the effects of probiotics on the immunological response to vaccination against bacterial respiratory diseases in young Japanese Black calves.

Neuropeptide nesfatin-1, a nucleobindin-2 derivative, plays a role in regulating food intake, energy metabolism and body weight. It also interacts with the hypothalamic-pituitary-gonadal axis and has functions in the reproductive system. However, its impact on the canine reproductive tract has not been well documented. This study aimed to investigate the potential role of nesfatin-1 in canine ovarian activity and uterine function.

The aim of the study was to explore and characterise healthy foals’ eye microbiomes in the first two months of life.

Periodontal diseases are the most frequently diagnosed problem in small animal veterinary medicine. Although their exact cause is not fully understood, bacteria play an important role in their development. Pseudomonas aeruginosa is a Gram-negative, rod-shaped, non-spore-forming bacterium. The living environment of this bacterium may be soil and water; however, it can also be found in humans and animals. Antibiotic treatment of periodontitis may be complicated by the carbapenem resistance of some P. aeruginosa strains, if these bacteria are found to be an aetiological agent. The aim of the study was to identify all bacterial strains isolated from dog with periodontitis.

Introduction In Germany, around 150,000 kg of mostly Siberian sturgeon (Acipenser baerii), were produced in 2021. Sudden mortalities affected negative control Russian sturgeon in experimental infection of several European aquacultured fish species with tilapia lake virus (TiLV). An investigation sought the causative agent. In most of the sturgeon, a specific herpesvirus was detected which also occurred in the carp, crucian carp and tench subjects, but not in Nile tilapia. This herpesvirus was latent in the sturgeon population but became productive to cause the outbreak after three weeks of experimentation. Material and Methods Different European aquacultured fishes were experimented upon. Chosen PCRs, nested PCRs and re-amplifications were carried out to identify the causative agent of the mortality event. Sequence analysis of the obtained PCR fragment and in-situ hybridisation (ISH) using tissue sections of the experimental fishes were performed. Results The PCRs used for detection of Acipenser herpesvirus (AciHV)-1 and -2 were always negative. An additional PCR assay with lesser specificity for AciHV found 118 of 123 sturgeon samples and some samples of cohabited cyprinids positive. The similarity of all isolates was 99.7%. The PCR results were confirmed by ISH using probes based on the same sequence, which detected identical viral sequences in both sturgeon and cyprinid samples. These findings revealed that a sterlet sequence previously deposited in the NCBI database had been incorrectly classified. Conclusion It seems that different herpesviruses and/or a new subspecies of AciHV are widespread in European farmed sturgeon populations, which, at least for aquacultured fish, opens up the possibility of vaccination against the disease which they cause. Additionally, a more specific diagnostic PCR has to be established.

Over the past three decades, emerging epidemiological evidence has shown the increasing incidence and prevalence of nontuberculous mycobacteria (NTM). As a result, clinical awareness of the impact these organisms have on both human and animal health has grown.

Staphylococci commonly colonise the skin and mucous membranes of humans and animals, the close contact between which may promote interspecies transmission of these microorganisms and determinants of drug resistance.