This paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major regulatory sequences is insufficient, as in genetic engineering manipulations 35S promoter – of DNA-containing cauliflower mosaic virus (CaMV) is used, which presence in the test material can lead to false positive results. In this work the feasibility of testing for the presence of viral CaMV’s DNA and analysis of screened gene sequences of interest for the usage during the analysis of rapeseed (Brassica napus L.) samples. It was established that the analysis of the presence/absence of a positive result for the CaMV 35S promoter is not giving complete answers to the absence of GMO in the sample. The appropriateness of GMO’s screening in rape seeds with NOS terminator and genes of interest.

This paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major regulatory sequences is insufficient, as in genetic engineering manipulations 35S promoter – of DNA-containing cauliflower mosaic virus (CaMV) is used, which presence in the test material can lead to false positive results. In this work the feasibility of testing for the presence of viral CaMV’s DNA and analysis of screened gene sequences of interest for the usage during the analysis of rapeseed (Brassica napus L.) samples. It was established that the analysis of the presence/absence of a positive result for the CaMV 35S promoter is not giving complete answers to the absence of GMO in the sample. The appropriateness of GMO’s screening in rape seeds with NOS terminator and genes of interest.

Purpose. Determine the main indicators of productivity and quality of seeds of spring rapeseed new varieties during their cultivation in different argoclimatic zones of Ukraine. Methods. Field, laboratory. Field studies were carried out on the basis of the Branches of the Ukrainian Institute for Plant Variety Examination in the zones of Polissia, Forest-Steppe and Steppe in 2015–2016. Results. The State Regis­ter of Plant Varieties Suitable for Distribution in Ukraine (the Register of Plant Varieties of Ukraine) as at 22 August 2019 contains 60 varieties of spring rapeseed, of which 42 (70.0%) are of foreign origin and 18 (30.0%) are Ukrainian cultivars. The maximum sown area in Ukraine occupied by spring rapeseed was about 68.3 thousand ha in 2018, which is twice as much as the area of 2013. In 2019, a dramatic decrease in sown area was observed. The productivity of the studied rapeseed varieties in 2015–2016 in the Steppe zone was on average 0.94–1.17 t/ha; Forest-steppe – 2.16–2.29; Polissia – 1.33–1.62. The yields of individual varieties exceeded 2.0 t/ha; they had a high content of oil and crude protein and a low content of erucic acid and glucosinolates in the seeds. The content of erucic acid did not exceed 0.1%, glucosinolates – 0.8%. Conclusions. The highest yield in the zones of Forest-Steppe and Polissia was observed from ‘DK 7160 KL’ variety – 2.28 t/ha and 1.62 t/ha, respectively. The maximum crude protein content in the steppe zone was found in ‘Aksana’ variety (26.6%), in the Forest-steppe zone – in ‘Bilder’ (25.4%,) in the Polissia zone – in ‘Sander’ (24.9%). High oil content in seeds in the Steppe zone was in varieties ‘Bilder’ (45.0%) and ‘DK 7155 KL’ (45.2%), in the Forest-Steppe – in ‘Sander’ (46.5%) and ‘DK 7155 KL’ (46.6%); in Polissia – in ‘DK 7160 KL’ (47.1%) and ‘DK 7155 KL’ (46.8%). The minimum content of erucic acid in the seeds of spring rapeseed in the Steppe was in the varieties ‘Aksana’ and ‘DK 7150 KL’, in the Forest-Steppe – ‘GK 7160 KL’, ‘CLICK KL’, ‘DK 7155 KL’ and in the Polissia zone – ‘DK 7160 KL’ and ‘DK 7150 KL’.

Purpose. Determine the main indicators of productivity and quality of seeds of spring rapeseed new varieties during their cultivation in different argoclimatic zones of Ukraine. Methods. Field, laboratory. Field studies were carried out on the basis of the Branches of the Ukrainian Institute for Plant Variety Examination in the zones of Polissia, Forest-Steppe and Steppe in 2015–2016. Results. The State Regis­ter of Plant Varieties Suitable for Distribution in Ukraine (the Register of Plant Varieties of Ukraine) as at 22 August 2019 contains 60 varieties of spring rapeseed, of which 42 (70.0%) are of foreign origin and 18 (30.0%) are Ukrainian cultivars. The maximum sown area in Ukraine occupied by spring rapeseed was about 68.3 thousand ha in 2018, which is twice as much as the area of 2013. In 2019, a dramatic decrease in sown area was observed. The productivity of the studied rapeseed varieties in 2015–2016 in the Steppe zone was on average 0.94–1.17 t/ha; Forest-steppe – 2.16–2.29; Polissia – 1.33–1.62. The yields of individual varieties exceeded 2.0 t/ha; they had a high content of oil and crude protein and a low content of erucic acid and glucosinolates in the seeds. The content of erucic acid did not exceed 0.1%, glucosinolates – 0.8%. Conclusions. The highest yield in the zones of Forest-Steppe and Polissia was observed from ‘DK 7160 KL’ variety – 2.28 t/ha and 1.62 t/ha, respectively. The maximum crude protein content in the steppe zone was found in ‘Aksana’ variety (26.6%), in the Forest-steppe zone – in ‘Bilder’ (25.4%,) in the Polissia zone – in ‘Sander’ (24.9%). High oil content in seeds in the Steppe zone was in varieties ‘Bilder’ (45.0%) and ‘DK 7155 KL’ (45.2%), in the Forest-Steppe – in ‘Sander’ (46.5%) and ‘DK 7155 KL’ (46.6%); in Polissia – in ‘DK 7160 KL’ (47.1%) and ‘DK 7155 KL’ (46.8%). The minimum content of erucic acid in the seeds of spring rapeseed in the Steppe was in the varieties ‘Aksana’ and ‘DK 7150 KL’, in the Forest-Steppe – ‘GK 7160 KL’, ‘CLICK KL’, ‘DK 7155 KL’ and in the Polissia zone – ‘DK 7160 KL’ and ‘DK 7150 KL’.

Purpose. Determine the main indicators of productivity and quality of seeds of spring rapeseed new varieties during their cultivation in different argoclimatic zones of Ukraine. Methods. Field, laboratory. Field studies were carried out on the basis of the Branches of the Ukrainian Institute for Plant Variety Examination in the zones of Polissia, Forest-Steppe and Steppe in 2015–2016. Results. The State Regis­ter of Plant Varieties Suitable for Distribution in Ukraine (the Register of Plant Varieties of Ukraine) as at 22 August 2019 contains 60 varieties of spring rapeseed, of which 42 (70.0%) are of foreign origin and 18 (30.0%) are Ukrainian cultivars. The maximum sown area in Ukraine occupied by spring rapeseed was about 68.3 thousand ha in 2018, which is twice as much as the area of 2013. In 2019, a dramatic decrease in sown area was observed. The productivity of the studied rapeseed varieties in 2015–2016 in the Steppe zone was on average 0.94–1.17 t/ha; Forest-steppe – 2.16–2.29; Polissia – 1.33–1.62. The yields of individual varieties exceeded 2.0 t/ha; they had a high content of oil and crude protein and a low content of erucic acid and glucosinolates in the seeds. The content of erucic acid did not exceed 0.1%, glucosinolates – 0.8%. Conclusions. The highest yield in the zones of Forest-Steppe and Polissia was observed from ‘DK 7160 KL’ variety – 2.28 t/ha and 1.62 t/ha, respectively. The maximum crude protein content in the steppe zone was found in ‘Aksana’ variety (26.6%), in the Forest-steppe zone – in ‘Bilder’ (25.4%,) in the Polissia zone – in ‘Sander’ (24.9%). High oil content in seeds in the Steppe zone was in varieties ‘Bilder’ (45.0%) and ‘DK 7155 KL’ (45.2%), in the Forest-Steppe – in ‘Sander’ (46.5%) and ‘DK 7155 KL’ (46.6%); in Polissia – in ‘DK 7160 KL’ (47.1%) and ‘DK 7155 KL’ (46.8%). The minimum content of erucic acid in the seeds of spring rapeseed in the Steppe was in the varieties ‘Aksana’ and ‘DK 7150 KL’, in the Forest-Steppe – ‘GK 7160 KL’, ‘CLICK KL’, ‘DK 7155 KL’ and in the Polissia zone – ‘DK 7160 KL’ and ‘DK 7150 KL’. | Мета. Визначити основні показники продуктивності та якості насіння нових сортів ріпаку ярого за вирощування їх у різних аргокліматичних зонах України. Методи. Польовий, лабораторний. Польові дослідження проводили на базі Філій Українського інституту експертизи сортів рослин у 2015–2016 рр. в зоні Полісся, Лісостепу та Степу. Результати. У Державному реєстрі сортів рослин, придатних для поширення в Україні (Реєстр сортів рослин України) станом на 22.08.2019 р. міститься 60 сортів ріпаку ярого типу розвитку, з яких 42 (70,0%) – іноземної селекції та 18 (30,0%) – вітчизняної селекції. Максимальна посівна площа в Україні зайнята ріпаком ярим була в 2018 році на рівні 68,3 тис. га, що вдвічі більше площ 2013 року. У 2019 році спостерігалось кардинальне зниження посівних площ. Урожайність досліджуваних сортів ріпаку за 2015–2016 рр. у зоні Степу була в середньому 0,94–1,17 т/га; Лісостепу – 2,16–2,29; Поліссі – 1,33–1,62. Урожайність окремих сортів перевищувала 2,0 т/га, вони мали високий вміст олії та сирого протеїну і низький вміст ерукової кислоти та глюкозинолатів у насінні. Вміст ерукової кислоти не перевищував 0,1%, глюкозинолатів – 0,8%. Висновки. Максимальна урожайність у зонах Лісостепу і Полісся була в сорту ‘ДК 7160 КЛ’ – 2,28 т/га та 1,62 т/га відповідно. Максимальний вміст сирого протеїну в Степовій зоні встановлено в сорту ‘Аксана’ – 26,6%, у зоні Лісостепу в ‘Білдер’ – 25,4%, у зоні Полісся в ‘Сандер’ – 24,9%. Високий вміст олії в насінні в зоні Степу формували сорти: ‘Білдер’ – 45,0% та ‘ДК 7155 КЛ’ – 45,2%, у Лісостепу ‘Сандер’ – 46,5% та ‘ДК 7155 КЛ’ – 46,6%; у Поліссі ‘ДК 7160 КЛ’ – 47,1% та ‘ДК 7155 КЛ’ – 46,8%. Мінімальний вміст ерукової кислоти в насінні ріпаку ярого у Степу був у сортів – ‘Аксана’, ‘ДК 7150 КЛ’, у Лісостепу – ‘ДК 7160 КЛ’, ‘КЛІК КЛ’, ‘ДК 7155 КЛ’ та в зоні Полісся – ‘ДК 7160 КЛ’, ‘ДК 7150 КЛ’. | Цель. Определить основные показатели продуктивности и качества семян новых сортов рапса ярового при выращивании их в разных аргоклиматических зонах Украины. Методы. Полевой, лабораторный. Полевые исследования проводили на базе Филиалов Украинского института экспертизы сортов растений в 2015–2016 гг. в зоне Полесья, Лесостепи и Степи. Результаты. В Государственном реестре сортов растений, пригодных для выращивания в Украине (Реестр сортов растений Украины) по состоянию на 22.08.2019 г. содержится 60 сортов рапса ярового типа развития, из которых 42 (70,0%) – иностранной селекции и 18 (30,0%) – отечественной селекции. Максимальная посевная площадь в Украине, занятая рапсом яровым, была в 2018 году на уровне 68,3 тыс. га, что вдвое больше площадей 2013 года. В 2019 году наб­людалось кардинальное снижение посевных площадей. Урожайность изучаемых сортов рапса в 2015–2016 гг. в зоне Степи была в среднем 0,94–1,17 т/га, Лесостепи – 2,16–2,29, Полесье – 1,33–1,62. Урожайность отдельных сортов превышала 2,0 т/га, они имели высокое содержание масла и сырого протеина и низкое содержание эруковой кислоты и глюкозинолатов в семенах. Содержание эруковой кислоты не превышало 0,1%, глюкозинолатов – 0,8%. Выводы. Максимальная урожайность в зоне Лесостепи и Полесья была у сорта ‘ДК 7160 КЛ’ – 2,28 и 1,62 т/га соответственно. Максимальное содержание сырого протеина в Степной зоне установлено у сорта ‘Аксана’ – 26,6%, в зоне Лесостепи –‘Билдер’ – 25,4%, в зоне Полесья – ‘Сандер’ – 24,9%. Высокое содержание масла в семенах в зоне Степи формировали сорта: ‘Билдер’ – 45,0% и ‘ДК 7155 КЛ’ – 45,2%, в Лесостепи ‘Сандер’ – 46,5% и ‘ДК 7155 КЛ’ – 46,6%, в Полесье ‘ДК 7160 КЛ’ – 47,1% и ‘ДК 7155 КЛ’ – 46,8%. Минимальное содержание эруковой кислоты в семенах рапса ярового в Степи было у сортов: ‘Аксана’, ‘ДК 7150 КЛ’, в Лесостепи: ‘ДК 7160 КЛ’, ‘КЛИК КЛ’, ‘ДК 7155 КЛ’ и в зоне Полесья: ‘ДК 7160 КЛ’, ‘ДК 7150 КЛ’.

The article highlights economical value of rape, an important bioenergy crop. It offers statistical data on rape hybrids release into the market.

The article highlights economical value of rape, an important bioenergy crop. It offers statistical data on rape hybrids release into the market.

The article represents results of researches of 20082010 years and evaluation of the new registered and promising varieties and hybrids winter rape (seed) by their genetic potential productivity and adaptability. Changeability varieties according to high-yielding properties and elements of productivity depending with adaptability to the environmental conditions of areas of Forest, Forest-steppe and Steppe of Ukraine. There are discussed the prospects of the trends.

The article represents results of researches of 20082010 years and evaluation of the new registered and promising varieties and hybrids winter rape (seed) by their genetic potential productivity and adaptability. Changeability varieties according to high-yielding properties and elements of productivity depending with adaptability to the environmental conditions of areas of Forest, Forest-steppe and Steppe of Ukraine. There are discussed the prospects of the trends.

Advantages of heterotic hybrids of the winter rape in the competitive and in the production testing proven. While certain advantages in the productivity of cultivar of the Czech selection of Odila above the domestic cultivar of Dangal got in the competitive testing in the production testing confirmed. It is established that the greatest productivity in the production testing was had hybrids Vsesvit F1t Zapalnyj Fb G-3 Fi and G-4 Fi, which are characterized the stable and yearly high productivity and combine the productivity with adaptability to the environmental conditions of area of Forest- steppe of Ukraine.

Advantages of heterotic hybrids of the winter rape in the competitive and in the production testing proven. While certain advantages in the productivity of cultivar of the Czech selection of Odila above the domestic cultivar of Dangal got in the competitive testing in the production testing confirmed. It is established that the greatest productivity in the production testing was had hybrids Vsesvit F1t Zapalnyj Fb G-3 Fi and G-4 Fi, which are characterized the stable and yearly high productivity and combine the productivity with adaptability to the environmental conditions of area of Forest- steppe of Ukraine.