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Biochemical features of the introduced population of Serratula coronata L. (Asteraceae) in Central Polissia of Ukraine
2019
Іващенко, І. В | Рахметов, Д. Б | Вергун, О. М
Purpose. To establish the biochemical composition of the above ground part of Serratula coronata L. (Crowned saw-wort) for introduction in Central Polissia of Ukraine. Methods. The object of research was the plants of S. coronata from the collection of the Botanical garden of Zhytomyr National Agroecological University. Plant raw material was evaluated in the flowering phase in the biochemical laboratory of the Department of Cultural Flora of the M. M. Hryshko National Botanic Gardens of the National Academy of Sciences of Ukraine according to the relevant methods during 2014–2016. Results. The results of the study of phytochemical features of S. coronata under conditions of introduction in the Central Polissia of Ukraine in the flowering phase are given. Quantitative content of raw material in dry matter, total sugars, carotene, ascorbic acid, tannins, fats, free acids, macroelements of phosphorus, calcium and trace elements of iron, copper, zinc, manganese was revealed. Conclusions. The biochemical composition of the above ground part of S. coronata was determined for the first time in the conditions of introduction in Central Polissia of Ukraine. The peculiarities of the dependence of the content of biochemical compounds and macroelements on age characteristics of plants were determined. Plants of S. coronata of the third year of life were distinguished by the highest content of ascorbic acid, carotene and dry matter; two-year – organic acids, phosphorus, ash, common sugars; the fourth year of life – the content of oil and calcium. Significant amounts of vitamin C and iron were found in the raw material. The obtained results testify to the prospect of further study of the pharmacological properties of S. coronata in order to obtain new food products, bio-additives and phytopreparations enriched with biologically active substances and essential for human life.
Show more [+] Less [-]Phytomeliorative properties of <i>Cannabis sativa</i> L. plants depending on varietal features of the culture
2017
Кабанець, В. М | Михальська, Л. М | Швартау, В. В | Матус, В. М
Purpose. To investigate the varietal characteristics of the hemp plants for improving the quality indices of the soil they grow in, determine the amount of inorganic elements in the soil, level of their accumulation in plant stalkі and seeds. Methods. Field and spectrometric methods were basic. The results were processed using conventional methods in agriculture, crop growing and statistics. Variants of the experiment were as follows: varieties ‘Hliana’, ‘Hlesiia’: 1) soils; 2) stalks; 3) seeds. Schemes of experiments included: a) technical maturity of plants, row spacing 45 cm; b) technical maturity of plants, row spacing 15 cm; c) biological maturity of plants, row spacing 45 cm; d) biological maturity of plants, row spacing 15 cm. Results. The amount of the accumulation of alkaline earth metals and their compounds by seeds and stalks of hemp plants depending on their content in vegetation soils was determined. It was found that stalks of the ‘Hlesiia’ plant accumulated strontium (Sr) and its compounds far less than that of ‘Hliana’, whereas in the seeds of the ‘Hlesiia’ variety the content of this chemical element was higher comparing with the previous variety by 70 and 78%, respectively. The difference in the accumulation of barium (Ba) compounds in seeds of hemp plants was not significant, while the tissues of the plant stalks of the ‘Hliana’ variety accumulated its compounds significantly more as compared to the ‘Hlesiia’ variety. The degree of influence of the variety, feeding area and the maturity stage on the processes of magnesium compounds (Mg) accumulation by plants was not revealed. Plants of the ‘Hlesiia’ variety accumulated far less calcium (Ca) and its compounds in the stalk tissues as compared to the plants of the ‘Hliana’ variety: in variants of the technical maturity stage of plants with row spacing 45 cm (a) and d – plants of narrow-row sowing (15 cm) in the biological maturity stage 30,94 and 15,95 mg/kg more in the presence in soil and in variants of the technical maturity stage of plants of narrow-row sowing (15 cm) and in the biological maturity stage with broad-sowing (45 cm) 34.54 and 24.19 mg/kg less in the presence in soil. Conclusions. The indices of accumulation of alkaline earth metals by hemp plants were significantly affected by the concentration of compounds of a certain chemical element in the arable layer, the level of energy (light) obtained by plants during vegetation, the varietal features of hemp, the stages of organogenesis of hemp plants and the specificity of their aboveground parts – stalks to accumulate these chemical elements as well as cumulate them by seeds. The varietal dependence as for accumulation of heavy metals by hemp plants tissues and seeds was established. In order to obtain environmentally friendly products, it is necessary to take into account the varietal features of plants concerning the ability to absorb and accumulate the relevant chemical elements and their compounds in the process of hemp cultivation.
Show more [+] Less [-]Features of accumulation of inorganic elements in seeds of white mustard (Sinapis alba L.) and black mustard (Brassica nigra L.)
2016
Рудник-Іващенко, О. І | Михальська, Л. М | Швартау, В. В
Purpose. To investigate special aspects of accumulation of inorganic elements including heavy metals in seeds of white and black mustard to be grown for obtaining drugs. Methods. Field experiments, microwave digestion, ICP-MS and statistical analysis. Results. The content of inorganic elements including heavy metals was determined in the seeds of white and black mustard grown in Kiev Oblast. It was revealed that during the growing season plants of white mustard were able to accumulate such elements as aluminum, barium, strontium, zinc in seeds in concentrations that exceed their content in black mustard seeds, while compounds of calcium, cesium, iron, magnesium, manganese, potassium in a greater degree were accumulated in black mustard seeds. Conclusions. As legal and regulatory documents for important chemical elements don’t contain the maximum permissible limits of their content in medicinal plants, it would make sense to launch a comprehensive research with the involvement of specialists of relevant profiles in order to establish such a gradation. Plants of white and black mustard in Kiev Oblast have accumulated high levels of such metals as Ba, Cu, Fe, Mn, Mo, Sr, Zn that exceed the known limits of accumulation, indicating a partial contamination of soils in the region. Consequently, these plants can be used for phytoremediation of soils. Considering the fact that in the pharmaceutical practice refined mustard seed oil is used, revealed alterations of metal accumulation in seeds will not affect the quality of the final drugs. According to the research results, white and black mustard is promising for cultivation in Kiev Oblast with a view to obtain raw material that can be processed into drugs.
Show more [+] Less [-]Study of some biochemical parameters and productivity of Silphium L. genotypes as perspective energetic crops
2020
Рахметов, Д. Б | Вергун, О. М | Рахметова, С. О | Шиманська, О. В | Фіщенко, В. В
Purpose. This study was aimed to determine some biochemical parameters and productivity of the gene fund of Silphium L. genus in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine. Methods. Plant raw material investigated at the flowering stage (17 genotypes) and the end of vegetation (20 genotypes) of Silphium spp. 3 species and 4 cultivars studied for the content of nutrients at the flowering. Determination of dry matter, ash, calcium, nitrogenfree extractives conducted according to Hrytsaienko et al. (2003), phosphorus, protein – according to Pochinok (1976), sugars – according to Krishchenko (1983), cellulose – according to Zheng et al. (2018), lipids – according to Zamowski, Suzuki (2004). It was used productivity parameters: yield of above-ground mass, the yield of dry mass, energetic value, yield of energy. Data analyzed statistically. Results. Investigation of nutrients content showed that content of dry matter was in the range of 18.90–29.3%, protein – in the range of 8.88–23.56%, cellulose – 15.10–36.14%, ash – 8.13–12.19%, lipids – 1.83–3.97%; yield of above-ground mass – 45.0–139.0 t/ha, the yield of dry matter – 10.31–36.92 t/ha, energy value – 3933–4249 cal/g, and yield of energy – 43.81–149.27 Gcal/ha. A study of genotypes at the flowering and end of vegetation identified that the content of dry matter for all samples was in a range of 18.38–67.49%, sugars – 2.78–19.0%, ash – 3.93–11.20%, calcium – 1.68–5.99%, phosphorus – 0.14–1.21%, energy value – 3153.36– 3770.28 cal/g. Conclusions. Plant raw material of genotypes of Silphium L. spp. is a valuable source of nutrients. The content of ash, its components, energetic value, and parameters of productivity depending on genotype and stage of growth. The results allow recommending selected Silphium genotypes as perspective energetic crops in Ukraine as well as other countries.
Show more [+] Less [-]Morphometric and biochemical features of different Bunias orientalis L. genotypes in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine
2021
Vergun, O. M. | Rakhmetov, D. B. | Shymanska, O. V. | Rakhmetova, S. O. | Bondarchuk, O. P. | Fishchenko, V. V.
Purpose. Determine a number of morphometric and biochemical parameters of various genotypes of Bunias orientalis L. in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG). Methods. Plant samples of B. orientalis (6 genotypes created in the NBG) were examined during the flowering stage. Determination of dry matter, ash, calcium was carried out according to Hrytsaienko et al. (2003), phosphorus according to Pochinok (1976), sugars, ascorbic acid and lipids were determined according to Krishchenko (1983), b-carotene according to Pleshkov (1985). The energy value of plants was determined using an IKA C-200 calorimeter. The obtained results were analysed statistically. Results. The height of plants varied from 140.9 (Genotype 1) to 157.5 (Genotype 5) cm, stem diameter from 11.67 (Genotype 1) to 16.1 (Genotype 6) mm, the number of internodes from 18.7 (Genotype 1) to 25.7 (Genotype 6), the number of leaves on a stem from 14.11 (Genotype 1) to 21.8 (Genotype 5), leaf lamina length from 14.2 (Genotype 1) to 23.45 (Genotype 6) cm, leaf lamina width from 6.34 (Genotype 1) to 14.5 (Genotype 4) cm, inflorescence length from 27.4 (Genotype 1) to 45.4 (Genotype 3) cm, inflorescence width from 2.32 (Genotype 1) to 4.92 (Genotype 3) cm, and the number of stems from 2.55 (Genotype 2) to 5.33 (Genotype 1). The study of the content of structural and functional compounds and nutrients at the flowering stage showed that the dry matter content was in the range of 13.58–16.00%, sugars 5.07–8.86%, titratable acidity 3.28–4.25%, lipids 3.33–6.61%, ascorbic acid 382.83–693.82 mg%, b-carotene 0.94–3.48 mg%, ash 6.79–9.2%, calcium 1.00–2.44%, phosphorus 1.61–2.67% and energy value 3337.0–3498.0 cal/g. Conclusions. It was revealed that samples of various genotypes of B. orientalis are a valuable source of nutrients at the flowering stage. The biochemical composition of plants depended on the genotype and stage of growth. Results of the morphometric study showed variability of investigated parameters. The obtained data can be used to predict and evaluate the results of introduction and breeding studies with B. orientalis genotypes as promising crops in Ukraine.
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