Purpose. Defining marker-trait associations of microsatellite markers with specific regions of the genome that control important agronomical traits in the investigated varieties originated in the Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigations and entered into the State register of plant varieties suitable for dissemination in Ukraine during different years. Methods. Molecular genetic methods (extraction of genomic DNA, polymerase chain reactions (PCR), electrophoresis of amplification products in polyacrylamide gel), field methods (phenological observations of heading date and analysis of plant height, visual assessment of the colour and length of wheat ear and awns), statistical methods (evaluation of trait means by descriptive statistic instruments of EXCEL package, ANOVA method performed by GLM instrument from AGROBASE 21 package). Results. During four gro­wing years (2010/11, 2011/12, 2012/13, 2013/14), 47 bread winter wheat varieties were phenotypically measured and analyzed with 17 microsatellite loci. 35 marker-trait associations (MTA) for heading date, 39 for plant height, 33 for awn size, 20 for ear colour and 8 for ear size were found to be stable and significant during two–four different gro­wing years. Conclusions. Microsatellite markers that showed substantial and stable during different growing years associations with agronomical traits can be useful and suitable for marker-assisted selection (MAS) in Ukrainian wheat breeding programs.