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Characteristics of new varieties of tomato (Solanum lycopersicum L.)
2016
Ю. О. Люта | Н. О. Кобиліна
Purpose. To analyse biochemical and economic characters of new tomato varieties adapted to the conditions of the south of Ukraine. Methods. Field study, laboratory testing, statistical analysis. Results. Intraspecific hybridization method followed by individual selection was used to create such new tomato varieties as ‘Naddnіprianskyi 1’, ‘Inguletskyi’, ‘Kimmeriiets’, ‘Sarmat’, ‘Taim’, ‘Legin’, ‘Kumach’ adapted to the conditions of the south, which are characterized by high yielding (69,2–78,0 t/ha), fruit quality (content of soluble dry matter 5,65–5,92%), drought (7,2–8,1 points) and heat (70,4–88,2%) resistance. All varieties are of intensive type, they require advanced agricultural technology, irrigation. Conclusions. New tomato varieties, namely ‘Naddnіprianskyi 1’, ‘Inguletskyi’, ‘Kimmeriiets’, ‘Sarmat’, ‘Taim’, ‘Legin’, ‘Kumach’ were entered in the State register of plant varieties suitable for dissemination in Ukraine. They are recommended for outdoor growing in the Steppe and Forest-Steppe zones of Ukraine.
Show more [+] Less [-]On streamlining the Ukrainian names of plants. Information 6. Names of some subtribe Malinae Reveal taxa (information 6)
2016
В. М. Меженський
Purpose. To analyse the Ukrainian nomenclature of subtribe Malinae representatives and improve terminological system of the Ukrainian names of a species and a generic levels and names of fruit crops. Results. There is a confusion in the Ukrainian names of plants of Cydonia-Chaenomeles–Pseudocydonia, Mespilus–Eriobotrya, Amelanchier–Cotoneaster. Considering the division of Sorbus s.l. on separate genera Sorbus s.str., Aria, Cormus, Chamaemespilus, Torminalis and availability of numerous hybrids with their involvement, the Ukrainian names for both taxa and nothotaxa of species and generic levels are offered, principles of their choice are also considered. Formation of the Ukrainian generic names on the basis of specific epithets leads to undesirable tautonomy. Aspects of derussification of the Ukrainian professional terminology are discussed. Conclusions. Ukrainian scientific generic and nothogeneric plant names for subtribe Malinae are transliterated/transcripted, borrowed as a calque of latin names or have their own origin, consisting of one or two words. The choice of the best variants of generic and species names from a number of possible ones has a temporal component and largely depends on experts who apply them and basic principles of choice. It is necessary to improve the modern Ukrainian plant nomenclature on a national basis involving the transformed Latin scientific names for introduced species and hybrids.
Show more [+] Less [-]Varietal features of lectin activity of soft winter wheat (Triticum aestivum L.) at the early stages of ontogenesis
2016
Л. В. Чеботарьова
Purpose. To study the dynamics of soft winter wheat lectin activity in caryopsis and at the early stages of ontogenesis of varieties developed by Poltava State Agrarian Academy as well as investigate varietal differences depending on dynamic pH medium. Methods. Laboratory analysis, method of hemagglutination reaction, analytical approach, statistical evaluation. Results. A change in lectin activity was determined in samples of 14 soft winter wheat varieties in caryopsis and at the early stages of ontogenesis (on the third, seventh, tenth and twelfth day of seedlings emergence). High lectin activity was observed in samples of seven and ten days seedlings. This could be evidence of active participation of proteins in physiological and biochemical processes at the time of emerging wheat tillering node that can be important in forming adaptive reactions to stress. Wheat lectin activity variation for рН ranging between 4,0 and 8,0 that depended on a variety was defined for the first time. Conclusions. According to hemagglutination ability of wheat lectins, all varieties were divided into three groups: of high ability – 4 varieties, middle – 9 varieties, low – 1 variety. Varietal variability for the investigated trait can be used in breeding programs as marker trait. In most varieties variation of hemagglutination was observed at dilutions ranging between 1:2 and 1:256. Availability of two lectin agglutination peaks in acidic and alkaline media for seedlings was fixed.
Show more [+] Less [-]Evaluation of the genus Calendula L. diversity for forming collection of varieties with standard characters
2016
Мельничук, Р. В | Куценко, Н. І
Purpose. Evaluation of collection samples of the genus Calendula L. available at the Experimental station of medicinal plants (ESMP) for the levels of display of morphological characters in order to identify the reference varieties and form a collection of standards. Methods. Field, laboratory, mathematical and statistical ones. Results. During 2012–2014 years the collection of the genus Calendula L. was evaluated, that includes 145 samples of 4 species from 18 countries, for the eight morphological characters (plant height, inflorescence number, full inflorescence number, the color of ligulate and tubular florets, number of rows of ligulate florets, inflorescence diameter, disk diameter) and varieties were distributed by the level of manifestation according to current technique for distinctness, uniformity and stability (DUS). Conclusions. Samples of marigold were identified that can be sources of standards or starting material by plant height, inflorescence number, inflorescence full number, color of ligulate and tubular florets, number of rows of ligulate florets, inflorescence diameter, disk diameter and by all degree of their manifestation. For the highest level of manifestation of character complex (inflorescence diameter, disk diameter, the number of rows of ligulate florets, inflorescence full number and their manifestation pattern) ‘Mandarin Twist’ variety appeared to be the best
Show more [+] Less [-]Evaluation of the genus Calendula L. diversity for forming collection of varieties with standard characters
2016
Р. В. Мельничук | Н. І. Куценко
Purpose. Evaluation of collection samples of the genus Calendula L. available at the Experimental station of medicinal plants (ESMP) for the levels of display of morphological characters in order to identify the reference varieties and form a collection of standards. Methods. Field, laboratory, mathematical and statistical ones. Results. During 2012–2014 years the collection of the genus Calendula L. was evaluated, that includes 145 samples of 4 species from 18 countries, for the eight morphological characters (plant height, inflorescence number, full inflorescence number, the color of ligulate and tubular florets, number of rows of ligulate florets, inflorescence diameter, disk diameter) and varieties were distributed by the level of manifestation according to current technique for distinctness, uniformity and stability (DUS). Conclusions. Samples of marigold were identified that can be sources of standards or starting material by plant height, inflorescence number, inflorescence full number, color of ligulate and tubular florets, number of rows of ligulate florets, inflorescence diameter, disk diameter and by all degree of their manifestation. For the highest level of manifestation of character complex (inflorescence diameter, disk diameter, the number of rows of ligulate florets, inflorescence full number and their manifestation pattern) ‘Mandarin Twist’ variety appeared to be the best
Show more [+] Less [-]Агрономічна оцінка стійкості італійських сортів твердої пшениці до фузаріозу колоса та скринінг удосконалених за допомогою MAS ліній, відібраних за ознакою стійкості до фузаріозу колоса
2016
Г. Бентівенга | М. Камеріні | А. Белoччі | М. Форнара | С. Меллоні | А. Спіна | Ф. Каранта | К. Аммар
Для оцінки стійкості до фузаріозу колоса в 2009 році 41 сорт твердої та м’якої пшениці, переважно з Італії, пройшов сортовипробування у CIMMYT (Міжнародний центр поліпшення кукурудзи та пшениці). Крім того, виконано оцінку впливу одного з основних QTL стійкості до фузаріозу колоса (Qfhs.ndsu-3BS QTL), вперше виявленого у китайського сорту пшениці м’якої ‘Sumai 3’, на хромосомі 3В, у 125 удосконалених ліній пшениці твердої BC4F6, отриманих шляхом схрещування з вихідним сортом пшениці м’якої ‘Sumai 3’ (68 ліній з ‘Sumai 3’ QTL та 57 ліній без цього QTL), були досліджені в однакових умовах штучного зараження. Для обох груп ділянки заражували під час цвітіння суспензією односпорових культур F. graminearum, підтримуючи вологість до 100%, щоб сприяти розвитку захворювання за допомогою системи дрібнодисперсного зволоження. Через тридцять днів після зараження підрахували кількість колосків, інфікованих F. graminearum, на колосі десяти рослин на кожній ділянці; пошкодження виразили показником зараження фузаріозом (кількість випадків ураження ступінь ураження / 100, де ступінь ураження = кількість інфікованих колосків / загальна кількість випадків ураження 100 та кількість інфікованого колосся / загальна кількість колосся 100). В обох випадках пізнє цвітіння було ключовим чинником, здатним обмежити ураженість хворобою. Попередні дані стосовно впливу Qfhs.ndsu-3BS QTL не виявили відмінності між двома групами вдосконалених ліній.
Show more [+] Less [-]Development of multiplex PCR system for identification of glyphosate-tolerant sugar beet
2016
Л. М. Присяжнюк | Ю. В. Шитікова | О. О. Волчков
Purpose. To create a multiplex system for identification glyphosate-tolerant sugar beet by using PCR. Methods. Molecular genetic analysis. Results. The article presents the results of studies to determine the parameters of the polymerase chain reaction (PCR) in order to develop a multiplex system for identification of the structural elements of the design of transgenic gene cp 4 epsps, which provides tolerance to glyphosate. For amplicon target DNA sequences, the following values of temperature conditions of PCR were determined: step 1 (initial denaturation) 95 °C – 3 min; step 2 (specific reaction products accumulation): denaturation 95 °C – 45 s; hybridization of primers 55 °C – 50 s; elongation 72 °C – 1 min; number of cycles – 40; step 3 (final elongation) 72 °C – 6 min. A series of PCR were carried out for the purpose of selecting the optimal amount of DNA matrix for efficient estimate of transgenic sugar beet plants for the presence of specific sequences. Conclusions. To identify transgenic glyphosate-tolerant sugar beet, it is advisable to determine 35S promoter and gene cp 4 epsps in individual genotypes. It was found that during the selection of temperature parameters of multiplex reaction a 5 °C rise in primer hybridization temperature did not affect the identification of gene als that allowed to include specific primers for determination of this sequence as an internal control. Based on the results of test multiplex reactions, concentrations of dNTPs and Mg2+ ions were determined that allowed to exclude the possibility of non-specific fragments and false-negative results. The optimum amount of matrix DNA (100–150 ng) for an efficient estimate of transgenic sugar beet plants for the presence of specific sequences was determined. Obtained results allowed to develop a multiplex test system for identification of transgenic glyphosate-tolerant sugar beet which can be used for simultaneous determination of the 35S promoter, cp 4 epsps gene and als gene as an internal reaction control.
Show more [+] Less [-]Breeding value of the second generation of soybean populations for «growing season» trait
2016
О. З. Щербина | В. Г. Михайлов | О. О. Тимошенко | С. О. Ткачик
Purpose. Studying the inheritance of such trait of soybean (Glucine max (L.) Merrill) as growing season length in F2 and assessing hybrid combinations to identify more quick-ripening phenotypes as compared to parents. Methods. Laboratory test, mathematico-statistical evaluation. Results. In most crossbreeding combinations, when parents differed by growing season length, late ripeness was dominated in F2, in one combination – early ripeness, in two combinations, when parents scarcely differed by growing season length, complementary effect was observed for this index. It was found that ‘Anzhelika’/‘Mageva’ combination generated the highest number of more quick-ripening forms than any of the parents (13.1%), a smaller number was identified in ‘Legenda’/‘Vizhion’ (6.4%) and ‘Anzhelika’/‘Gentleman’ (4.0%), and barely noticeable number was observed in ‘Legenda’/‘Yelena’ combination (1.3%). Conclusions. In the following crossbreeding combinations as ‘Legenda’/‘Vizhion’, Legenda’/‘Korado’, ‘Legenda’/‘Ustia’, ‘Legenda’/‘Yelena’, ‘Yug-30’/‘Gentleman’, ‘No. 894’/‘Vizhion’, ‘Anzhelika’/‘Annushka’, ‘No. 894’/‘Annushka’, ‘Legenda’/‘Annushka’, ‘No. 441’/‘Gentleman’, ‘No. 441’/‘Vizhion’, ‘No. 441’/‘Annushka’, ‘Anzhelika’/‘Gentleman’ and ‘Anzhelika’/‘Prypiat’ when parents considerably and insignificantly differ by growing season length, late ripeness was dominated in F2. ‘Ustia’/‘Vizhion’ and ‘Yug-30’/‘ Vizhion’ crossbreeding combinations in which parents hardly differ by growing season, complementary effect was observed in F2 for this index.
Show more [+] Less [-]Complex prospective evaluation of “Romantica” roses introduction under the conditions of the Right-Bank Forest-Steppe Zone of Ukraine
2016
О. К. Мороз | Л. І. Дениско
Purpose. Theoretical and experimental substantiation of introduction of “Romantica” roses originated by Meilland company under the conditions of the Right-Bank Forest-Steppe Zone of Ukraine. Methods. Biometrical, comparative-morphological, statistic ones. Results. Viability, growth and development indices as well as ornamental qualities (tolerance for winter conditions, frost hardiness, drought resistance, complex disease and pest resistance, habit maintenance, shoot formation, flowering, coloration, shape of flowers, petal number etc.) were analyzed for 12 rose cultivars of “Romantica” series planted on the collection and exhibition plots of the National Dendrological Park «Sofiivka» NAS of Ukraine. The investigations were conducted within 2012–2015. The most of the cultivars got the mark of introduction success 41–45 points of 50; so, the cultivars are totally promising for introduction into the conditions of the Right-Bank Forest-Steppe Zone of Ukraine. ‘Leonardo da Vinci’ and ‘Yves Piaget’ cultivars got point number 35–38 and they can be considered promising ones. The acclimatization number for the most of the investigated cultivars was 85–90 points of 100 that means good adaptation of the plants in the conditions of the Right-Bank Forest-Steppe Zone of Ukraine. The adaptation of cv. ‘Leonardo da Vinci’ and cv. ‘Yves Piaget’ roses (point number 75) can be considered satisfactory. The total evaluation of “Romantica” roses ornamental qualities made 76–87 points of 100; so, all the cultivars display high ornamental qualities and deserve to be introduced to the production. Conclusions. The garden roses of “Romantica” series originated by Meilland company being introduced into the conditions of the Right-Bank Forest-Steppe Zone of Ukraine are adapted well in the introduction region and they are promising for the subsequent introduction.
Show more [+] Less [-]On streamlining the Ukrainian names of plants. Information 7. Spelling the names of plant varieties.
2016
В. М. Меженський
Purpose. To analyse the practice of transliteration of the Ukrainian cultivar names and rendering foreign names by means of the Ukrainian language, as well as special aspects of cultivar names spelling in special literature. Results. Cultivar names as a special category require preservation of primary graphics or sound type in the other language. This can be achieved by direct inclusion of the original name to the Ukrainian text or by practical transcribing, but not by transliteration or translation. Otherwise, Ukrainian names should be transliterated for inclusion to the texts in Latin characters. Transcription/transliteration in both directions is performed from the source language, though, as practice shows, in some Ukrainian publications the Russian is wrongly used as an intermediary language. Some national scientific publications ignore the recommendations of the International Code of Nomenclature for Cultivated Plants that is not conducive to the success of scientific communication in the globalized world. Conclusions. The foreign names of plant varieties should be entered into the Ukrainian text keeping the original spelling or by means of practical transcription. The loan of foreign names is performed by transcribing directly from the source language; if the language doesn’t have the Latin alphabet, Latinized name transcription is acceptable. Recommendations of the International Code of Nomenclature for Cultivated Plants that concern graphic highlighting of the cultivar names in the text enclosing them in single quotation marks and writing each word of a cultivar name with a capital letter should necessarily be applied in the foreign-language publications and extended to the Ukrainian special literature, at least, in terms of the use of single quotation marks. Ukrainian names should be transliterated only in accordance with the regulations.
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