Purpose. Investigation of maize ae1 gene polymorphism by bioinformatic methods. Methods. Global and local alignment of the nucleotide and amino acid sequences, in silico translation and transcription, translates modeling, pri­mers design, phylogenetic analysis. Results. 255 nucleotide sequences of maize аe1 gene, 500 amino acid sequences of homology translates of maize ae1 gene (SBEIIb enzyme homologs) and 100 mRNA expressed from the maize ae1 gene were analyzed to establish phylogenetic relationships. Polymorphism of maize ae1 gene different regions was investigated by bioinformatic methods. Modeling of the maize enzyme SBEIIb was performed. Conclusions. According to the results of amino acid sequences of SBEIIb enzyme homologs alignment, it was found that ae1 gene orthologs are pre­sent only in monocots, paralogs – in monocots, dicots, and other taxa, including algae and animals. Based on the results of alignment of plants mRNA from which enzyme SBEIIb is translated, maize ae1 gene orthologs and the nearest paralogs encoding starch branching enzymes with chloroplast localization were defined; this suggests a possible origin of ae1 gene due to duplication of the gene encoding the 1,4-alpha-glucan-branching enzyme 2 with chloroplast or amyloplast localization. In the maize ae1 gene structure, regions were found that include polymorphic sites not defined previously. For the polymorphic sites design primers were developed that allowed to differentiate the maize lines. It was determined that the detection of polymorphism in theory can influence the enzyme function and, as a result, change the concentration of amylopectin in maize grain.

Purpose. Investigation of maize ae1 gene polymorphism by bioinformatic methods. Methods. Global and local alignment of the nucleotide and amino acid sequences, in silico translation and transcription, translates modeling, pri­mers design, phylogenetic analysis. Results. 255 nucleotide sequences of maize аe1 gene, 500 amino acid sequences of homology translates of maize ae1 gene (SBEIIb enzyme homologs) and 100 mRNA expressed from the maize ae1 gene were analyzed to establish phylogenetic relationships. Polymorphism of maize ae1 gene different regions was investigated by bioinformatic methods. Modeling of the maize enzyme SBEIIb was performed. Conclusions. According to the results of amino acid sequences of SBEIIb enzyme homologs alignment, it was found that ae1 gene orthologs are pre­sent only in monocots, paralogs – in monocots, dicots, and other taxa, including algae and animals. Based on the results of alignment of plants mRNA from which enzyme SBEIIb is translated, maize ae1 gene orthologs and the nearest paralogs encoding starch branching enzymes with chloroplast localization were defined; this suggests a possible origin of ae1 gene due to duplication of the gene encoding the 1,4-alpha-glucan-branching enzyme 2 with chloroplast or amyloplast localization. In the maize ae1 gene structure, regions were found that include polymorphic sites not defined previously. For the polymorphic sites design primers were developed that allowed to differentiate the maize lines. It was determined that the detection of polymorphism in theory can influence the enzyme function and, as a result, change the concentration of amylopectin in maize grain.

Purpose. To study biometric characteristics of climbing roses leaves (length of compound leaf, the number of leaf blades, total leaf area and leaf blade area) and select the best varieties on this base. Methods. General biological (biometric) study and statistical evaluation. Results. As follows from the morphological analysis, a significant leaf size variation in the studied varieties of climbing roses was revealed. The leaf size in such varieties as ‘Kahovka’, ‘Flammentanz’, ‘Krasnyi Maiak’ was the largest, in ‘New Dawn’ and ‘Krymskoe Solnyshko’ – the smallest one. Based on the leaf biometric study of 9 varieties of climbing roses, the authors defined that ‘Flammentanz’, ‘Krasnyi Maiak’, ‘Kahovka’ varieties had the highest values of leaf parameters. The ‘Veilchenblau’ variety differed significantly from the other studied samples by the form and color of leaves that had a positive impact on its decorative value. Data obtained allowed to select varieties that can be used in landscaping not only because of flower decorative characteristics, but also due to attractive leaf appearance, as well as varieties for application in breeding programs related to this trait. Conclusions. Biometric characteristics of climbing roses leaves (length of compound leaf, the number of leaf blades, total leaf area and leaf blade area) were studied, and in compliance with the results the best varieties were selected that can be re­commended for use in gardening and breeding.

Цель. Проанализировать сортовой потенциал селекции, оптимизацию сортовой структуры производства и периодичность сортосмены табака (Nicotiana tabacum L.) в Республике Болгария.Результаты. Ретроспективный анализ селекции табака (Nicotiana tabacum L.) показывает, что за период 1950–2010 гг. в Болгарии созданы 158 сортов, из которых 130 – подвида Восточный (ssp. Orientalis), и 28 сортов подвида Американский (ssp. Americana), среди которых 17 сортов типа Вирджиния и 10 сортов типа Берли. На базе этого сортового потенциала проведены соответствующие сорто­смены в производственном секторе страны. За 50–60 лет (1950–2010 гг.) произведено 5–7 сортосмен отечественными сортами. Высокая эффективность селекции с получением качественных сортов и сравнительно частые сорто­смены создают благоприятные возможности для ежегодного оптимального формирования сортовой структуры производства в зависимости от требований и спроса соответствующего сырьевого сегмента рынка и быстро меня­ющейся рыночной конъюнктуры.Выводы. Из общего сортового потенциала селекции сортов табака подвида Восточный (130 сортов) за 13-летний период (1995, 2000–2011 гг.) вошли в производственный оборот 47,7% (62 сорта), около 25% (24–30 сортов) формируют основной объем Восточного табака, который ежегодно производится в Болгарии. Сортовой потенциал селекции Крупнолистных сортов полностью использован в производстве – 17 сортов типа Вирджиния и 10 сортов типа Берли. Табак является единственной сельскохозяйственной культурой в Болгарии, которая включает в структуру производства почти 100% сортов отечественной селекции. | Мета. Проаналізувати сортовий потенціал селекції, оптимізацію сортової структури виробництва та періодичність сортових змін тютюну Nicotiana tabacum L. у Республіці Болгарія.Результати. Ретроспективний аналіз селекції тютюну (Nicotiana tabacum L.) свідчить, що за період 1950–2010 рр. у Болгарії створено 158 сортів, серед яких 130 сортів з підвиду Східний (ssp. Orientalis), 28 сортів підвиду Американський (ssp. Americana), з яких 17 сортів типу Вірджинія і 11 сортів типу Берлі. На основі цього сортового потенціалу проведено відповідні сортозміни у виробничому секторі країни. За 50–60 років (1950–2010 рр.) здійснено 5–7 сортозмін вітчизняними сортами. Висока ефективність селекції тютюну з отриманням якісних сортів і порівняно часті сортозміни створюють сприятливі можливості для щорічного оптимального формування сортової структури виробництва залежно від вимог і попиту відповідного сировинного сегменту ринку та кон’юнктури ринку, що швидко змінюється.Висновки. Із загального сортового потенціалу селекції сортів тютюну підвиду Східний (130 сортів) за 13-річний період (1995, 2000–2011 рр.) увійшли до виробничого обороту 47,7% (62 сорти), приблизно 25% (24–30 сортів) формують основний обсяг Східного тютюну, який щорічно виробляють у Болгарії. Сортовий потенціал селекції великолистового тютюну у виробництві використано повністю – 17 сортів типу Вірджинія та 11 сортів типу Берлі. Тютюн є єдиною сільськогосподарською культурою в Болгарії, яка включає в структуру виробництва майже 100% сортів вітчизняної селекції. | Purpose. To analyze the varietal potential of breeding, the optimization of the varietal structure of production and the frequency of changes in the commonly used varieties of tobacco (Nicotiana tabacum L.) in the Republic of Bulgaria.Methods. Comparative, statistical, analytical ones.Results. The retrospective analysis of tobacco (Nicotiana tabacum L.) breeding in Bulgaria shows that 158 varieties were created during the period of 1950–2010 including 130 varieties of the Oriental subspecies (ssp. Orientalis) and 28 ones of the American subspecies (ssp. Americana), 17 of the latter are of the Virginia type and 11 are of the Burley type. Based on this varietal potential, appropriate variety changing were periodically adopted by the production sector in the country. There were about 5–7 variety changing during 50–60 years (1950–2010) using domestic varieties. The high efficiency of tobacco breeding with the creation of high-quality varieties and the relatively frequent variety changing are prerequisites for optimal formation of the varietal structure of production on a yearly basis depending on preferences and requirements of the particular raw market segment and the rapid changes in the market condition.Conclusions. From the total varietal potential of the Oriental tobacco breeding (130 varieties), for a period of 13 years, 47.7% (62 varieties) were used in production, while approximately 25% (24–30 varieties) constitute the main volume of Oriental tobacco produced in Bulgaria every year. The varietal potential of breeding of large-leaved tobacco has been fully realized in production – 17 varieties of Virginia type and 11 varieties of Burley type. Tobacco is the only agricultural crop in Bulgaria whose production structure includes almost 100% locally selected varieties.

Purpose. Developing technology for clonal micropropagation of peppermint (Mentha piperita L.) plants of Ukrainian breeding based on the complex of methods of isolated tissue and organ culture in vitro. Methods. During the experiment, such methods as isolated tissue and organ culture in vitro, clonal micropropagation, detached scion grafting, chemotherapy with adding of virucide Ribavirin to the nutrient medium, biometric and statistical ones were used. Results. The stepped procedure of sterilization that we have developed allows to receive 88–100% of sterile explants. For M. piperita L. introduction into culture and clonal micropropagation, Murashige and Skoog (MS) nut­rient medium appeared to be optimal supplemented with 6-benzylaminopurine (0.75 mg/l), adenine (0.05 mg/l), indole-3-acetic acid (IAA) (0.05 mg/l) and gibberellic acid (0.5 mg/l) on which the reproduction ratio on the 28th day ranged between 1:7 and 1:15. For recovery of plants from viral infection, virucide Ribavirin at concentration of 10 mg/l was added to the nutrient medium. The proposed nutrient medium for rhizogenesis, that contained IAA (0.5 mg/l) and indole butyric acid (IBA) (0.5 mg/l), allows to obtain the frequency of rhizogenesis up to 84–100%. Regenerated plants were adapted to the conditions in vivo on substrate peat : universal soil : perlite : sand in the ratio 2:1:1:1. The survival rate for peppermint varieties amounted to 96–100%. Conclusions. Biotechnological scheme was developed that permits to get healthy, purebred planting material and intensively propagate plants for supplying breeding programs of the Experimental Station for Medicinal Plants of the Institute of Agroecology and Environmental Management of the National Academy of Agrarian Sciences of Ukraine, among which such varieties as ‘Lebedyna pisnia’ and ‘Ukrainska pertseva’ were selected as the most promising for clonal micropropagation.

The article researched genetic potential, morpho-biological characteristics and features of new varieties of winter wheat varieties of semi-dwarf and short-stem types. It was established that new generation of such varieties displayed improved architectonic of plants, as well as a complex of morphological and economical value chararacteristics and features, combined high genetic potential of productivity with good adopting features, and short stem with high resistance to drowning, and generated higher crop capacity compared to conventional mid-ripening varieties.

Purpose. Studying variety samples of winter triticale of various ecological and geographical origin for revealing polymorphism of the culture for its susceptibility to pathogenic complex of Fusarium head blight and defining high-yielding and resistant to diseases varieties that later can be put into the production and breeding process. Methods. Field experiments, statistical evaluation. Results. It was defined that the development of Fusarium head blight in different variety samples of winter triticale depends on the hydrothermal conditions and genotype of the host-plant. Resistance of varieties and hybrids to the disease was partial, and no immune samples were found among the analyzed ones. The following varieties proved to be tolerant to Fusarium head blight: ‘Granat’, ‘Zorro’, ‘Obrii Myronivskyi’. The positive correlation between the development of Fusarium head blight and root rot of winter triticale was found. It was determined that in the pathogenic complex of Fusarium head blight the amount of the following pathogens is increasing: Fusarium sporotrichioides, F. аvenaceum and F. poae. Conclusions. The leading varieties that combine high yields and resistance to Fusarium head blight are as follows: ‘Obrii Myronivskyi’, ‘ADM 8’, ‘Yuvileine Volynske’, ‘Yukon’, ‘Zorro’, ‘Tsekad 90’, ‘Zerniatko’, ‘Legion’ and ‘Rarytet’. These varieties should be involved in the selection process to breed the hybrids with the augmented resistance to the disease and high yields.

Purpose. Studying variety samples of winter triticale of various ecological and geographical origin for revealing polymorphism of the culture for its susceptibility to pathogenic complex of Fusarium head blight and defining high-yielding and resistant to diseases varieties that later can be put into the production and breeding process. Methods. Field experiments, statistical evaluation. Results. It was defined that the development of Fusarium head blight in different variety samples of winter triticale depends on the hydrothermal conditions and genotype of the host-plant. Resistance of varieties and hybrids to the disease was partial, and no immune samples were found among the analyzed ones. The following varieties proved to be tolerant to Fusarium head blight: ‘Granat’, ‘Zorro’, ‘Obrii Myronivskyi’. The positive correlation between the development of Fusarium head blight and root rot of winter triticale was found. It was determined that in the pathogenic complex of Fusarium head blight the amount of the following pathogens is increasing: Fusarium sporotrichioides, F. аvenaceum and F. poae. Conclusions. The leading varieties that combine high yields and resistance to Fusarium head blight are as follows: ‘Obrii Myronivskyi’, ‘ADM 8’, ‘Yuvileine Volynske’, ‘Yukon’, ‘Zorro’, ‘Tsekad 90’, ‘Zerniatko’, ‘Legion’ and ‘Rarytet’. These varieties should be involved in the selection process to breed the hybrids with the augmented resistance to the disease and high yields.

Purpose. Reporting peculiarities of new forms of edible corn and prospects for their use in food production that have been created jointly by scientists from the Plant Production Institute nd. a. V. Ya. Yuryev of NAAS and Ustymiv­ka Experimental Station for Plant Production on the base of the environmentally friendly method that uses biochemical effects of natural mutations of corn grain endosperm structure. Methods. Field study, laboratory analysis, mathematical and statistical evaluation. Results. Original series of inbred lines as well as promising varieties and hyb­rids of sugar and waxy corns were obtained. Varieties and hybrids are universally usable for obtaining both fresh and canned products, characterized by good taste, provide yields of up to 10 tons per hectare of commercial products and aimed to grow in various soil and climatic conditions. Brief economic and morphological characteristics of corn varieties and hybrids created jointly by scientists from the Plant Production Institute nd. a. V. Ya. Yuryev of NAAS and Ustymivka Experimental Station for Plant Production were given. Conclusions. The use of biochemical effect of natural mutations of endosperm structure is cost-effective and environmentally friendly method for creating lines, varieties and hybrids of corn for direct consumption and processing into food products. ‘Biliavka’, ‘Nika’ varieties and ‘Binom F1’, ‘Solo F1’, ‘Twister F1’ hybrids created with the help of this method are promising for commercial use

Purpose. Reporting peculiarities of new forms of edible corn and prospects for their use in food production that have been created jointly by scientists from the Plant Production Institute nd. a. V. Ya. Yuryev of NAAS and Ustymiv­ka Experimental Station for Plant Production on the base of the environmentally friendly method that uses biochemical effects of natural mutations of corn grain endosperm structure. Methods. Field study, laboratory analysis, mathematical and statistical evaluation. Results. Original series of inbred lines as well as promising varieties and hyb­rids of sugar and waxy corns were obtained. Varieties and hybrids are universally usable for obtaining both fresh and canned products, characterized by good taste, provide yields of up to 10 tons per hectare of commercial products and aimed to grow in various soil and climatic conditions. Brief economic and morphological characteristics of corn varieties and hybrids created jointly by scientists from the Plant Production Institute nd. a. V. Ya. Yuryev of NAAS and Ustymivka Experimental Station for Plant Production were given. Conclusions. The use of biochemical effect of natural mutations of endosperm structure is cost-effective and environmentally friendly method for creating lines, varieties and hybrids of corn for direct consumption and processing into food products. ‘Biliavka’, ‘Nika’ varieties and ‘Binom F1’, ‘Solo F1’, ‘Twister F1’ hybrids created with the help of this method are promising for commercial use

Мета. З’ясувати особливості формування адитивних біоценотичних зв’язків у фітоценозах тритикале озимого в умовах лісостепового та полісько-лісостепового екотопів. Методи. Польові, лабораторні дослідження та математично-статистичний аналіз. Результати. Досліджено чутливість генотипів тритикале озимого, зокрема АД 256, Славетне, Славетне поліпшене, Пшеничне, Чаян, Ягуар, Августо, ДАУ 5 і Вівате Носівське, на дію мікробних препаратів альбобактерину та діазобактерину в умовах лісостепового та полісько-лісостепового екотопів. Встановлено, що інтродукція агрономічно корисної мікробіоти в фітоценози тритикале озимого забезпечує формування адитивних біоценотичних зв’язків, ефективність яких визначає рівень продуктивності зернової культури. Висновки. Для забезпечення оптимальних цільових параметрів урожаю зерна в умовах лісостепового та полісько-лісостепового екотопів доцільно проводити передпосівну інокуляцію насіння тритикале озимого ефективними штамами мікробних препаратів – Azospіrіllum brasilense діазобактерину та Achromobacter album 1122 альбобактерину.

Мета. З’ясувати особливості формування адитивних біоценотичних зв’язків у фітоценозах тритикале озимого в умовах лісостепового та полісько-лісостепового екотопів. Методи. Польові, лабораторні дослідження та математично-статистичний аналіз. Результати. Досліджено чутливість генотипів тритикале озимого, зокрема АД 256, Славетне, Славетне поліпшене, Пшеничне, Чаян, Ягуар, Августо, ДАУ 5 і Вівате Носівське, на дію мікробних препаратів альбобактерину та діазобактерину в умовах лісостепового та полісько-лісостепового екотопів. Встановлено, що інтродукція агрономічно корисної мікробіоти в фітоценози тритикале озимого забезпечує формування адитивних біоценотичних зв’язків, ефективність яких визначає рівень продуктивності зернової культури. Висновки. Для забезпечення оптимальних цільових параметрів урожаю зерна в умовах лісостепового та полісько-лісостепового екотопів доцільно проводити передпосівну інокуляцію насіння тритикале озимого ефективними штамами мікробних препаратів – Azospіrіllum brasilense діазобактерину та Achromobacter album 1122 альбобактерину.

Purpose. To analyze the types of license agreements and their difference from other civil agreements, and requirements for the execution and termination of a license agreement. Results. It was revealed that the current legislation provides an inexhaustible list of the types of transactions with respect to disposition of intellectual property rights. The group of agreements on disposition of intellectual property rights includes the intellectual property license. The legislation provides for three types of license agreements under the terms of the exclusive, non-exclusive and single license. The result of a license agreement is to provide the intellectual property rights for temporary use without losing the copyright holder of this object. Conclusions. Proper execution of license agreements between licensor and licensee promotes protection of rights of each of the parties to such agreements. Licensee will have a legitimate reasons to use the variety, the licensee may receive compensation for granting the right to use the variety.

Purpose. To analyze the types of license agreements and their difference from other civil agreements, and requirements for the execution and termination of a license agreement. Results. It was revealed that the current legislation provides an inexhaustible list of the types of transactions with respect to disposition of intellectual property rights. The group of agreements on disposition of intellectual property rights includes the intellectual property license. The legislation provides for three types of license agreements under the terms of the exclusive, non-exclusive and single license. The result of a license agreement is to provide the intellectual property rights for temporary use without losing the copyright holder of this object. Conclusions. Proper execution of license agreements between licensor and licensee promotes protection of rights of each of the parties to such agreements. Licensee will have a legitimate reasons to use the variety, the licensee may receive compensation for granting the right to use the variety.

To analyse the modern classification and nomenclature of species of pome fruit crops which varieties are listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine, and improve terminological system of the Ukrainian names of both species and garden crops. Results. Fruit cultivars and most apple clonal rootstocks belong to Malus pumila, and ornamental cultivars belong to Malus gloriosa. The most common scientific name of the cultivated apple, especially among horticulturists, is Malus domestica, although according to the principle of priority the name Malus pumila should have the advantage. As far as Nomenclature Committee for Vascular Plants has rejected the proposal to conserve the name Malus domestica, Malus pumila is correct name for the cultivated apple. The use of synonymic name Malus domestica should be avoided in both scientific and scientific-popular papers for stability of nomenclature. Pear cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine are presented by Pyrus communis, and pear rootstocks – by Cydonia oblonga. Fruit cultivars of the latter belong to separate fruit crop named quince. An apple-quince hybrid was registered as universal clonal rootstock for pome fruit crops. The State Register of Plants Varieties Suitable for Dissemination in Ukraine also contains nonconventional fruit crops such as Chaenomeles and hawthorn that consist of some species and nothospecies. Conclusions. In scientific publications one should stop the use of synonymic name Malus domestica in favour of the correct name for cultivated apple Malus pumila. Apple, pears and Chaenomeles cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine have a complex multispecies origin whereas quince, hawthorn and pear roostock cultivars systematically are monospecies. A universal roootstock of pome fruit crops is Cydolus, or apple-quince, that resulted from interspecies hybridization between quince and apple. Refusal Refuse to use obsolete names of fruit plants species will promote harmonization of professional language.

To analyse the modern classification and nomenclature of species of pome fruit crops which varieties are listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine, and improve terminological system of the Ukrainian names of both species and garden crops. Results. Fruit cultivars and most apple clonal rootstocks belong to Malus pumila, and ornamental cultivars belong to Malus gloriosa. The most common scientific name of the cultivated apple, especially among horticulturists, is Malus domestica, although according to the principle of priority the name Malus pumila should have the advantage. As far as Nomenclature Committee for Vascular Plants has rejected the proposal to conserve the name Malus domestica, Malus pumila is correct name for the cultivated apple. The use of synonymic name Malus domestica should be avoided in both scientific and scientific-popular papers for stability of nomenclature. Pear cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine are presented by Pyrus communis, and pear rootstocks – by Cydonia oblonga. Fruit cultivars of the latter belong to separate fruit crop named quince. An apple-quince hybrid was registered as universal clonal rootstock for pome fruit crops. The State Register of Plants Varieties Suitable for Dissemination in Ukraine also contains nonconventional fruit crops such as Chaenomeles and hawthorn that consist of some species and nothospecies. Conclusions. In scientific publications one should stop the use of synonymic name Malus domestica in favour of the correct name for cultivated apple Malus pumila. Apple, pears and Chaenomeles cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine have a complex multispecies origin whereas quince, hawthorn and pear roostock cultivars systematically are monospecies. A universal roootstock of pome fruit crops is Cydolus, or apple-quince, that resulted from interspecies hybridization between quince and apple. Refusal Refuse to use obsolete names of fruit plants species will promote harmonization of professional language.

Purpose. Considering ways to solve issues related to soil cover study at the institutions for plant varieties examination. Methods.Fieldandanalytical ones. Results. Issues are identified that arise during soil sampling at institutions for plant varieties examination within the state system of plant varieties protection. The author considered in detail the current soil sampling techniques for agrochemical tes­ting for humus, nitrogen (compounds to be easily hydrolyzed), mobile P2O5, exchangeable K2O, hydrolytic acidity and others. Attention was paid to violation of methodological requirements concerning the time of soil sampling and number of samples for agrochemical testing at the experimental field, to the lack of devices for meteorological observation and appliances for soil and leaf diagnostics. The article also touches upon the issues associated with the study of soil cover at fields with scientifically-based crop rotation that belong to institutions for plant varieties examination within the state system of plant varieties protection. Conclusions. It should be necessary to follow recommended practice when sampling at elementary sites for agrochemical testing and performing all operations in accordance with crop cultivation flowcharts. General recommendations for agrochemical testing of fields in case of conventional crop rotations in farms are not suitable for fields with scientifically-based crop rotations at institutions for plant varieties examination.

Purpose. Considering ways to solve issues related to soil cover study at the institutions for plant varieties examination. Methods.Fieldandanalytical ones. Results. Issues are identified that arise during soil sampling at institutions for plant varieties examination within the state system of plant varieties protection. The author considered in detail the current soil sampling techniques for agrochemical tes­ting for humus, nitrogen (compounds to be easily hydrolyzed), mobile P2O5, exchangeable K2O, hydrolytic acidity and others. Attention was paid to violation of methodological requirements concerning the time of soil sampling and number of samples for agrochemical testing at the experimental field, to the lack of devices for meteorological observation and appliances for soil and leaf diagnostics. The article also touches upon the issues associated with the study of soil cover at fields with scientifically-based crop rotation that belong to institutions for plant varieties examination within the state system of plant varieties protection. Conclusions. It should be necessary to follow recommended practice when sampling at elementary sites for agrochemical testing and performing all operations in accordance with crop cultivation flowcharts. General recommendations for agrochemical testing of fields in case of conventional crop rotations in farms are not suitable for fields with scientifically-based crop rotations at institutions for plant varieties examination.

Purpose. Revealing of adaptive properties of plant varieties of red beet Bordeaux Kharkivskyi and Aktion based on the study of plant growth regulators for marketable production in the Steppe zone of Ukraine. Methods. Field, laboratory ones, variance analysis. Results. The influence of plant growth regulators (Lignogumat, Betastimulin) on earliness and productivity of such red beet varieties as Bordeaux Kharkivskyi and Aktion was established. Conclusions. Plant growth regulators (Betastimulin, Lignogumat) stimulated seed sprouting of red beet Bordeaux Kharkivskyi and Aktion varieties, activated vegetative plant growth increa­sing marketable yield. Application of Betastimulin (0.2%) for red beet Kharkivskyi Bordeaux and Aktion varieties provides the highest number of marketable beetroots, thus forming marketable yield of 44.7 and 54.9 t/ha respectively with the average beetroot weight of 328–519 g.

Purpose. Revealing of adaptive properties of plant varieties of red beet Bordeaux Kharkivskyi and Aktion based on the study of plant growth regulators for marketable production in the Steppe zone of Ukraine. Methods. Field, laboratory ones, variance analysis. Results. The influence of plant growth regulators (Lignogumat, Betastimulin) on earliness and productivity of such red beet varieties as Bordeaux Kharkivskyi and Aktion was established. Conclusions. Plant growth regulators (Betastimulin, Lignogumat) stimulated seed sprouting of red beet Bordeaux Kharkivskyi and Aktion varieties, activated vegetative plant growth increa­sing marketable yield. Application of Betastimulin (0.2%) for red beet Kharkivskyi Bordeaux and Aktion varieties provides the highest number of marketable beetroots, thus forming marketable yield of 44.7 and 54.9 t/ha respectively with the average beetroot weight of 328–519 g.