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Features of formation of Miscanthus giganteus planting material depending on cultivation technology elements
2017
В. А. Доронін | В. В. Дрига | Ю. А. Кравченко | В. В. Доронін
Purpose. To establish biological features of plants growth and development and the formation of Miscanthus giganteus planting material depending on the cultivation technology elements. Methods. Field, laboratory, visual, weight measuring, mathematical and statistical ones. Results. The features of the growth and development of the miscanthus bioenergy crop were investigated including the formation of planting material depending on the combined technology elements application during the planting time, namely: planting time, rhizome mass, the granules and the MaxiMarin absorbent gel. It was established that the increase in plant height and leaf area as well as the miscanthus stems formation was depended on both the rhizome planting time, their size, and the use of the absorbent. During three-year period, increase in plant height was more intensive and leaf area was largest in case of the absorbent application, as compared to the control during all phases of the development for the first and the second planting time regardless of rhizome mass. On the average, the largest leaf area – 1905,9 cm3 – was in the final stage of vegetation in the context of the second planting time for large rhizomes and application of granules and absorbent gel jointly. Increasing the ground mass due to plant height, leaf area and the number of stems benefited the photosynthesis productivity intensity, that influenced the root system increase, and consequently the output of the miscanthus planting material. It was found that there are direct strong correlation between these indices and the rhizome mass. Ground mass growing is contributed to the increase in the rhizome mass, and accordingly the output of the planting material – rhizome. In case of application of granules and absorbent gel jointly, the ground mass of the miscanthus was growing most intensively and accordingly the rhizome mass was the largest, which in the first year of small rhizomes planting was twice as much as compared to the control and was equal to 1090.5 g, for large rhizomes planting this index was respectively 2.4 times more and equal to 1763.9 g. During the second planting time, the application of granules and absorbent gel jointly resulted in the rhizomes mass increase for small rhizomes planting 1.9, large rhizomes – 2.1 times more as compared to the control. Conclusions. Direct strong correlations were established between the intensity of the ground mass growth – the height of plants, the number of leaves, leaf area, the number of buds and the rhizome mass. The growth of the ground mass of plants was contributed to the increase of the root system, and consequenly the output of planting material. In all stages of plant development, the increase of the rhizome mass was more intensive in case of the absorbent application regardless the time of rhizome planting, as compared to the control. The application of granules and absorbent jointly allowed to form the largest rhizome mass.
Show more [+] Less [-]Rice (<em>Oryza sativa</em> L.) blast resistance genes bioinformatic analysis
2017
Бондаренко, К. В | Сліщук, Г. І | Волкова, Н. Е
Purpose. To investigate rice blast resistance genes polymorphism by using bioinformatic methods. Methods. Global and local nucleotide alignment, phylogenetic analysis, HyPhy test. Results. For Pib gene, numerous single nucleotide substitutions and deletions of 1–3 bp were established. The phylogeny of this gene has been studied and homologues have been found both in various rice species and in other cereals. These sequences can encode proteins that «recognize» the phytopathogens effectors, and can also be associated with resistance to phytopathogens. The Pi4 gene is characterized by single nucleotide substitutions, insertions and deletions; the number of non-synonymous substitutions exceeds the number of synonymous ones. The Pi54 gene variability is significantly lower than that of the Pi4 and Pib genes. The predominant types of polymorphism were single nucleotide substitutions and small-sized indels. It was found that non-synonymous substitutions in Pi54, Pi4 and Pib genes were in close proximity, sometimes forming clusters, while some coding regions were either superconservative or contained predominantly synonymous substitutions. On philodendrograms, cultivated rice samples were clustered with samples of ancestral wild-growing species. Conclusions. Evolution of the rice blast resistance genes Pi4, Pib and Pi54 is characterized by diversification selection. Considering that tense coevolution and significant rate of adaptation and creation of new pathogen races are typical for a plant and a parasite, these genes are subjected to intensive selection aimed at increasing diversity for obtaining the resistance to new races of the pathogen.
Show more [+] Less [-]Forecasting of phenotypic productivity of middle-early soybean varieties
2017
Присяжнюк, О. І | Димитров, В. Г | Мартинов, О. М
Purpose. To study biological characteristics of growth and development of middle-early soybean varieties and create a model of phenotype productivity. Methods. Special and general techniques for studies. Results. Optimal productivity of plants is forming merely at the expense of efficient ratio of all the elements of their structure. It often happens that in case of underdevelopment of one of the structure components yield to some extent can be offset by better development of other elements. Eight indicators were defined which make the largest contribution to the productivity trait of a variety: seed weight per plant, total number of branches, number of nodes per plant, number of pods per plant, number of seeds per plant, number of flowers, plant height, 1000 kernel weight. The first four indicators provided most of the total contribution to a trait of seed weight per plant. Model building was based on hierarchy of productivity traits display in ontogenesis and compliance of their development in organogenesis. The model consists of two modules of traits – resulting and some componental showing phenotypic realization of the genetic formula. It was found that the plant height significantly influence the number of nodes per plant (r = 0,76), and the number of pods per plant (r = 0,43) depends on this trait. In addition, correlation based on the research was obtained between the number of flowers per plant and plant height (r = 0,35), and number of nodes (r = 0,76). It was established that the number of flowers per plant determines the development of pods on the plant so it is quite strongly correlated with this trait (r = 0,99). Conclusions. It was determined that the number of pods per plant and the number of seeds per plant have a very strong correlation (r = 0,96). Besides, such trait as the number of seeds per plant has a strong relationship with the seed weight per plant (r = 0,79).
Show more [+] Less [-]Dynamics of correlation relationship changes in new winter wheat varieties (<em>Triticum aestivum</em> L.) under the influence of environmental factors in the Southern Forest-Steppe zone of Ukraine
2017
М. М. Гаврилюк | П. Є. Каленич
Purpose. To establish correlation dependence of influence of such factors as seeding rate, sowing time and seeding methods on yield indicators, as well as individual response of winter wheat varieties to them in case of the ecological system of seed production, that would allow to form elements of intensive varietal technology and obtain the genetically determined productivity potential of the variety in the future. Methods. Weight measuring was used to determine seed yield; the reliability of the experiment results was identified by statistical evaluation, analysis of variance and regression procedure. Results. During the period of investigation, the weather conditions differed significantly both for the temperature regime and the amount of precipitation, but the stability of grain and seed yield over years was the main requirement to varieties, that allowed to study the intergenotypic correlation relationships of crop yield indices and the dynamics of their changes under the effect of ecological factors. In the course of investigation, winter wheat yield indices were fixed annually from 684 plots, which were grouped and analyzed for factors of influence in order to ensure the complete certainty. Conclusions. Variants of investigation were defined for the certified seed yield and environmental factors (sowing time and seeding method, seeding rate), that had strong positive correlation relationships. Seeding rate and seeding method had the greatest impact on 1000 kernel weight. For tillering coefficient, varieties depending on the variant of the investigation had a negative close correlation relationship, which was greatly affected by seeding rate. For correlation relationships, winter wheat varieties ‘Bohdana’ and ‘Slavna’ appeared to be the most stable, ‘Chorniava’ and ‘Astarta’ were the most plastic.
Show more [+] Less [-]Peculiarities of productivity formation of the genus Salix L. representatives
2017
В. В. Баликіна
Purpose. To identify the productivity of the genus Salix L. plants and study the relationship between its structural elements. Methods. Field study, laboratory analysis, analytical approach. Results. It was found that the height of a three-year plants of species ranged from 86.0 to 775.1 cm, of hybrid forms – from 197.0 to 488.0 cm. Average diameter of three-year plants varied in the range of 19.10 to 52.94 mm (species) and from 28.04 to 49.23 mm (hybrid forms). The highest stability for complex of morphological characters was observed in bog willow samples. It was determined that among species basket willow (16.94 t/ha) and white willow (21.19 t/ha) were the most productive for dry biomass yield per 1 hectare, among hybrid forms – purple willow × basket willow (23.36 t/ha) and basket willow × bog willow (18.57 t/ha). It was established that the value of the plants productivity was characterized by moderate, significant and close correlations with the average diameter of plants, length and number of shoots of the second order. Conclusions. A comprehensive assessment of productivity traits of three-year plants from willow collection was conducted, index of dry matter yield per 1 hectare was defined. The links between quantitative traits that characterize the contribution of some of them in productivity index were investigated. Method of cluster analysis was used to group samples for the similarity of complex agronomic characters. Basket willow and white willow as well as such hybrid forms as basket willow × bog willow and purple willow × basket willow were recommended to use as a source material for selection of samples with high productivity.
Show more [+] Less [-]Criteria of assessing introduced varieties of the genus Heuchera L.
2017
Н. А. Андрух
Purpose. To identify and describe the basic criteria for studying and assessing decorative and economic-and-biological characters of the genus Heuchera L. varieties in the context of introduction. Methods. Introduction procedure, analytical approach, morphological analysis and biometric data evaluation. Results. Aided by the comparative morphological analysis of introduced varieties of the genus Heuchera, significant differences in their characters were revealed, including plant height, height and width of the basal rosette of leaves, generative shoot height. There was a significant variation of such parameters as the number of generative shoots within one plant, the number of flowers on a single generative shoot, flowering abundance. Based on investigation findings, 17 groups of varieties were identified for the dominant color of adaxial surface of the leaf blade and 7 groups – for inflorescence color. According to phenological observations, Heuchera varieties groups were determined and the dates of commencement and duration of plants flowering were registered in the context of introduction. The results of these investigations are the necessary basis for the study and evaluation of this culture assortment, they are important in breeding and landscaping. Conclusions. Based on the results of investigations of morphological features of introduced species of the genus Heuchera, traits and parameters for varieties grouping were defined as well as decorative and economic-and-biological traits that should be evaluated.
Show more [+] Less [-]Prospects of domestic viticulture transition to ecological (adaptive) management
2017
І. А. Ковальова | Л. В. Герус | М. Г. Банковська | M. Г. Федоренко | О. В. Салій
Purpose. To determine the possibility of the domestic viticulture transition to the ecological (adaptive) management system, based on the use of highly adaptive varieties. Methods. Procedures are conventional in viticulture. All stages of agrobiological research were carried out according to the M. A. Lazarevskiy technique. The resistance of genetic resources to fungal diseases was assessed using the 9-point scale on the natural infectious background with maximum damage. Organoleptic evaluation of wine and fresh grapes was performed using 8-point and a 10-point scales respectively. Results. The main agrobiological traits (resistance to diseases, productivity and yield quality) of 22 perspective grape varieties and forms were studied. The level of resistance to fungal diseases was determined as one of the main criteria of the variety suitability for ecological (adaptive) viticulture. During five years, the average resistance level of this group of table and wine varieties was not lower than the relative one (6.5–7 points), and in ‘Zagrei’ variety – up to 7,5 points. The level of quality characteristics of products of new grape varieties and forms was determined (wine evaluation, marketability and assessment of fresh table grapes). Samples with a combination of high wine and grape quality characteristics and high productivity were selected. To replenish the gene pool of grapes by adaptive varieties, such perspective hybrid combinations as ‘Opalovyi’ ´ ‘Burmunk, ‘Avgustin’ ´ ‘Oryhinal and ‘Ohoniok tairovskyi’ ´ ‘Kardyshakh’ were studied. The level of group resistance to fungal diseases was identified and the perspective genotypes were preliminarily selected. Conclusions. The level of display of a number of economic characters in the group of perspective table and wine grape varieties and forms was determined. Highly adaptive and highly productive varieties and forms suitable for the use in the adaptive viticulture were defined. Prospective hybrid combinations were considered, from which highly adaptive varieties will be selected as suitable for the ecological system of viticulture.
Show more [+] Less [-]Response of new varieties of winter wheat (Triticum aestivum L.) to the impact of environmental factors under the conditions of the Southern Forest-Steppe zone of Ukraine
2017
М. М. Гаврилюк | П. Є. Каленич
Purpose. To define the level of impact of such factors as rate, time and methods of sowing on seed productivity and certified seeds, and the individual response of winter wheat varieties to these factors if used the ecological system of seed farming, that would allow to form and obtain genetically determined potential of variety productivity. Methods. Weight measurement for determining seed yield; statistical, variance and regression analysis for identifying the reliability of the experiment results. Results. During the research period, weather conditions were greatly differed both by temperature regime and precipitation, but stability of grain and seeds yield on an annual basis was the main requirement to varieties. During the study of «grain productivity» value and quantitative indices of winter wheat yield structure, the results from 684 plots were obtained which were grouped and analyzed for impact factors for complete certainty, and NIR0,05 was calculated. Conclusions. Thus, in case of shifting sowing time from optimum to later period, efficiency of water use by plants was decreasing to a greater extent during years with insufficient precipitation. The shift of sowing time to later period providing optimal seeding rate and row seeding method did not reduce yield. The yield of winter wheat varieties to be studied when sowing in usual manner with seeding rate of 5,5 million seeds/ha in the period from September 15 and October 5 was the highest. Varieties ‘Slavna’ and ‘Chorniava’ provided the highest grain productivity for seeding rate of 5,5 million seeds/ha and using row seeding method, with slight impact of sowing time factor. Grain yield of studied varieties showed negative response during the experiment when seeding rate decreased up to 2,5–3,0 million seeds/ha. ‘Astarta’ variety provided the highest productivity for certified seeds yield as compared to the control (from 1,59 to 3,38 t/ha). The variant of the experiment when sowing on October, 5 in usual manner with seeding rate of 5,5 million seeds/ha showed the highest productivity of 7,02 t/ha (+3,38 t/ha, or +92,9%). Shifting of sowing time for October, 5 resulted in a significant decrease in yields of certified seeds as compared to sowing time on September 15 and 25. Seed yield of studied varieties when sowing on October, 5 was higher than in case of sowing on September, 25. The highest percentage of certified seeds output in the studied varieties was observed for a seeding rate of 2,5–3,0 million seeds/ha. For seeding rate of 5,5 million seeds/ha for varieties with seeding time between September 15 and 25, the difference in grain yield was negligible. The winter wheat variety ‘Astarta’ provided the highest yield of seeds during the research years – 9,36 t/ha, the standard variety ‘Bogdan’ – the lowest one in the best variant of 5,55 t/ha.
Show more [+] Less [-]Influence of growth regulator on plant growth, development and yield formation of sunflower hybrids (F<sub>1</sub>) under the conditions of Southern Steppe zone of Ukraine
2017
О. А. Єременко | В. В. Калитка | С. М. Каленська
Purpose. To study the effect of AKM plant growth regulator on growth, development and yield formation of sunflower (Helianthus annuus L.) hybrids in hybridization plots under the conditions of the Southern Steppe zone of Ukraine. Methods. Laboratory tests, field study, statistical evaluation. Results. The results of studies devoted to determining the optimal AKM concentration for the treatment of seeds of the maternal and paternal lines, the effect of AKM on field germination, biometric parameters of sunflower plants, seed quality (F1) and yield are presented. Three hybrids of the Ukrainian selection, such as ‘Alpha’, ‘Logos’ and ‘Persei’ were studied during 2014–2016. Optimal concentration of AKM (0.0015 g/l) was defined. The vigor of seeds processed by AKM was higher than in check variety by 0.8–12.8 р.р. (♂); 0.4–10.7 p.p. (♀), laboratory germination – by 2.3–6.1 p.p. (♂); 3.5–6.2 p.p. (♀). In 2016, the sunflower plant height for all variants exceeded this parameter to be obtained for other years of the study. This could be explained by the fact that HTC in 2016 for the BBCH 00–39 period was 1.4 times higher than in 2015. In general, hybrids as the studied factor considerably influenced sunflower yield, and the share of the hybrid (factor A) influence is 33%. This should be considered when selecting hybrids for sunflower cultivation technologies in the Steppe zone of Ukraine. Conclusions. Hydrothermal conditions of the year had the maximum impact on the formation of seed quality and yield of sunflower plants of the hybrids under study, but when using AKM growth regulator for presowing seed treatment, this negative impact was reduced by an average of 23%.
Show more [+] Less [-]Morphoagrobiological properties and productivity of new soft winter wheat varieties under the conditions of Kirovohrad variety testing station
2017
О. Л. Уліч | Г. М. Каражбей | С. В. Козак | Ю. Ф. Терещенко | І. В. Коховська
Purpose. To study morphoagrobiological and adaptive properties, level of yielding capacity of recently registered soft winter wheat varieties of various ecological groups under agroecological conditions of Kirovohrad variety testing station. Methods. Field study, laboratory test, analytical procedure and statistical evaluation. Results. It was established that the yield level of is a key composite indicator of genotype adaptation to agroecological growing conditions. Experimental data indicate significant deviations of yield depending on the genotype and the year of study. During three years of experiments, yield depending of the variety ranged from 4.26 to 9.71 t/ha, such varieties as ‘CN Kombin’, ‘Estivus’, ‘Tradytsiia odeska’, ‘Mudrist odeska’, ‘Lil’ and ‘Fabius’ had higher yields. In case of dry weather conditions and unfavorable agro-ecological factors, the following varieties as ‘Mudrist odeska’, ‘Veteran’, ‘Lil’, ‘Tsentylivka’, ‘Fabius’, ‘Patras’, ‘Montrei’ have demonstrated good adaptive properties. Their yield has decreased by 9,2–19,0%, while in the varieties ‘Mahistral’, ‘Poltavka’, ‘Harantiia odeska’ and ‘Pokrova’ – by 34.4, 42.4, 45.2 and 50.6% accordingly. Conclusions. Investigated soft winter wheat varieties differ in morphoagrobiological characteristics, productivity, height, maturation period, adaptability as well as economic and agronomic value. According to the complex of such indices as productivity, agronomic characters and properties as well as adaptability, in the microzone of Kirovohrad variety testing station it is advisable to grow varieties ‘CN Kombi’, ‘Pokrova’, ‘Mudrist odeska’, ‘Veteran’ and ‘Lil’.
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