Purpose. To study the features of the productivity formation of broccoli hybrids depending on the methods of application of “5 element” nanofertilizer in the conditions of the Western Forest-Steppe of Ukraine.Methods. Field, laboratory, statistical.Results. With the introduction of “5 element” nanofertilizer, the head diameter of the ‘Batavia F1’ hybrid increased from 16.6 (var. 2) to 18.3 cm (var. 8), while in the control this indicator was 15.9 cm. The biggest head diameter in the hybrid ‘Orantes F1’ 18.9 and 18.7 cm was noted with foliar feeding with “5 element” nanofertilizer in the phase of 4–6 leaves + beginning of head formation + 10–12 days after the beginning of head formation and in the phase of the beginning of head formation + 10–12 days after the beginning of head formation. Accordingly, in the aforementioned variants, the head mass was 901 and 863 g, which is higher than the control (without treatment) by 166 and 128 g, or 22.3 and 17.4%.The highest yield of the ‘Batavia F1’ hybrid was obtained in 8 and 7 variants of the experiment – 33.5 and 31.9 t/ha, respectively, the yield increase was 5.2 and 3.6 t/ha, or 18.4 and 12.7%. In the hybrid ‘Orantes F1’ the highest yield (37.1 t/ha) was recorded when applying the “5 element” nanofertilizer in the 8th variant of the experiment, which is 6.6 t/ha higher than the control, or 21.6%. Compared to the ‘Batavia F1’ hybrid, the yield in this variant of the ‘Orantes F1’ hybrid was 3.6 t/ha, or 9.7%. The diameter and weight of the head, as well as the yield of the hybrid ‘Orantes F1’ were larger compared to the hybrid ‘Batavia F1’, due to its biological characteristics. A strong correlation (r = 0.97 and 0.96) was revealed between the yield and the diameter of the broccoli head in the ‘Batavia F1’ and ‘Orantes F1’ hybrids. The quality indicators of broccoli (dry matter, sum of sugars and vitamin C) in the ‘Orantes F1’ hybrid were higher compared to the ‘Batavia F1’ hybrid. A tendency towards a decrease in the concentration of nitrates in the heads of broccoli with an increase in the number of treatments with nanofertilizer “5 element” was revealed. The concentration of nitrates in the ‘Orantes F1’ hybrid was lower in all variants of the experiment in comparison with the ‘Batavia F1’ hybrid. The concentration of nitrates in the heads of broccoli in all variants of the experiment did not exceed TLV (400 mg/kg wet weight).Conclusions. With an increase in the number of foliar treatments with “5 element” nanofertilizer, the yield increased and the quality of broccoli hybrids improved. It was revealed that the yield of the ‘Orantes F1’ hybrid in all variants of the experiment was higher in comparison with the ‘Batavia F1’ hybrid. When applying nanofertilizer “5 element” in three stages – foliar feeding in the phase of 4–6 leaves + beginning of head formation + 10–12 days after the beginning of head formation – we got the highest yield of broccoli in the hybrid ‘Orantes F1’ – 37.1 t/ha, an increase over the control variant (without fertilizers) was 6.6 t/ha, or 21.6%. The ‘Batavia F1’ hybrid had a yield of 33.5 t/ha, an increase over the control variant – 5.2 t/ha, or 18.4%. | Мета. Вивчити особливості формування продуктивності гібридів капусти броколі залежно від способів застосування нанодобрива «5 еlement» в умовах Західного Лісостепу України.Методи. Польовий, лабораторний, статистичний.Результати. За внесення нанодобрива «5 еlement» збільшувався діаметр головки в гібрида ‘Batavia F1’ від 16,6 (вар. 2) до 18,3 см (вар. 8), тоді як на контролі цей показник становив 15,9 см. Найбільший діаметр головки в гібрида ‘Orantes F1’ 18,9 та 18,7 см відзначено за позакореневого підживлення нанодобривом «5 еlement» у фазі 4–6 листків + початок формування головки + через 10–12 діб після початку формування головок та у фазі початок формування головок + через 10–12 діб після початку формування головок. Відповідно у вищезгаданих варіантах маса головок становила 901 та 863 г, що вище за контроль (без обробки) на 166 та 128 г, або 22,3 та 17,4%. Найвищу врожайність капусти броколі гібрида ‘Batavia F1’ одержано у 8 та 7 варіантах досліду – 33,5 та 31,9 т/га відповідно, приріст урожаю становив відповідно 5,2 та 3,6 т/га, або 18,4 та 12,7%. У гібрида ‘Orantes F1’ найбільшу врожайність (37,1 т/га) відзначено за внесення нанодобрива «5 еlement» у 8 варіанті досліду, що вище за контроль на 6,6 т/га, або 21,6%. Порівняно з гібридом ‘Batavia F1’ урожайність на цьому варіанті у гібрида ‘Orantes F1’ була більшою на 3,6 т/га, або 9,7%. Діаметр та маса головки, а також урожайність у гібрида ‘Orantes F1’ були більшими, порівняно з гібридом ‘Batavia F1’, що зумовлено його біологічними особливостями. Виявлено сильний кореляційний зв’язок (r = 0,97 та 0,96) між урожайністю та діаметром головки капусти броколі в гібридів ‘Batavia F1’ та ‘Orantes F1’. Якісні показники капусти броколі (суха речовина, сума цукрів та вітамін С) у гібрида ‘Orantes F1’ були вищими порівняно з гібридом ‘Batavia F1’. Виявлено тенденцію до зменшення концентрації нітратів у головках капусти броколі зі збільшенням кількості обробок нанодобривом «5 еlement». Концентрація нітратів у гібрида ‘Orantes F1’ була меншою в усіх варіантах досліду, порівняно з гібридом ‘Batavia F1’. Уміст нітратного азоту в головках капусти броколі в усіх варіантах досліду не перевищував ГДК (400 мг/кг сирої маси).Висновки. Зі збільшенням кількості позакореневих обробок нанодобривом «5 еlement» підвищувалась урожайність та поліпшувалася якість продукції гібридів капусти броколі. Виявлено, що врожайність гібрида ‘Orantes F1’ у всіх варіантах досліду була  більша, порівняно з гібридом ‘Batavia F1’. За внесення нанодобрива «5 еlement» у три етапи – позакореневе підживлення у фазі 4–6 листків + початок формування головки + через 10–12 діб після початку формування головок –  одержали найбільшу врожайність капусти броколі в гібрида ‘Orantes F1’ – 37,1 т/га, приріст до контролю (без добрив) становив 6,6 т/га, або 21,6%. У гібрида ‘Batavia F1’ урожайність становила 33,5 т/га, приріст до контролю – 5,2 т/га, або 18,4%.

Purpose. To study the features of the productivity formation of broccoli hybrids depending on the methods of application of “5 element” nanofertilizer in the conditions of the Western Forest-Steppe of Ukraine. Methods. Field, laboratory, statistical. Results. With the introduction of “5 element” nanofertilizer, the head diameter of the ‘Batavia F1’ hybrid increased from 16.6 (var. 2) to 18.3 cm (var. 8), while in the control this indicator was 15.9 cm. The biggest head diameter in the hybrid ‘Orantes F1’ 18.9 and 18.7 cm was noted with foliar feeding with “5 element” nanofertilizer in the phase of 4–6 leaves + beginning of head formation + 10–12 days after the beginning of head formation and in the phase of the beginning of head formation + 10–12 days after the beginning of head formation. Accordingly, in the aforementioned variants, the head mass was 901 and 863 g, which is higher than the control (without treatment) by 166 and 128 g, or 22.3 and 17.4%.The highest yield of the ‘Batavia F1’ hybrid was obtained in 8 and 7 variants of the experiment – 33.5 and 31.9 t/ha, respectively, the yield increase was 5.2 and 3.6 t/ha, or 18.4 and 12.7%. In the hybrid ‘Orantes F1’ the highest yield (37.1 t/ha) was recorded when applying the “5 element” nanofertilizer in the 8th variant of the experiment, which is 6.6 t/ha higher than the control, or 21.6%. Compared to the ‘Batavia F1’ hybrid, the yield in this variant of the ‘Orantes F1’ hybrid was 3.6 t/ha, or 9.7%. The diameter and weight of the head, as well as the yield of the hybrid ‘Orantes F1’ were larger compared to the hybrid ‘Batavia F1’, due to its biological characteristics. A strong correlation (r = 0.97 and 0.96) was revealed between the yield and the diameter of the broccoli head in the ‘Batavia F1’ and ‘Orantes F1’ hybrids. The quality indicators of broccoli (dry matter, sum of sugars and vitamin C) in the ‘Orantes F1’ hybrid were higher compared to the ‘Batavia F1’ hybrid. A tendency towards a decrease in the concentration of nitrates in the heads of broccoli with an increase in the number of treatments with nanofertilizer “5 element” was revealed. The concentration of nitrates in the ‘Orantes F1’ hybrid was lower in all variants of the experiment in comparison with the ‘Batavia F1’ hybrid. The concentration of nitrates in the heads of broccoli in all variants of the experiment did not exceed TLV (400 mg/kg wet weight). Conclusions. With an increase in the number of foliar treatments with “5 element” nanofertilizer, the yield increased and the quality of broccoli hybrids improved. It was revealed that the yield of the ‘Orantes F1’ hybrid in all variants of the experiment was higher in comparison with the ‘Batavia F1’ hybrid. When applying nanofertilizer “5 element” in three stages – foliar feeding in the phase of 4–6 leaves + beginning of head formation + 10–12 days after the beginning of head formation – we got the highest yield of broccoli in the hybrid ‘Orantes F1’ – 37.1 t/ha, an increase over the control variant (without fertilizers) was 6.6 t/ha, or 21.6%. The ‘Batavia F1’ hybrid had a yield of 33.5 t/ha, an increase over the control variant – 5.2 t/ha, or 18.4%.

Purpose. To study the features of the productivity formation of broccoli hybrids depending on the methods of application of “5 element” nanofertilizer in the conditions of the Western Forest-Steppe of Ukraine. Methods. Field, laboratory, statistical. Results. With the introduction of “5 element” nanofertilizer, the head diameter of the ‘Batavia F1’ hybrid increased from 16.6 (var. 2) to 18.3 cm (var. 8), while in the control this indicator was 15.9 cm. The biggest head diameter in the hybrid ‘Orantes F1’ 18.9 and 18.7 cm was noted with foliar feeding with “5 element” nanofertilizer in the phase of 4–6 leaves + beginning of head formation + 10–12 days after the beginning of head formation and in the phase of the beginning of head formation + 10–12 days after the beginning of head formation. Accordingly, in the aforementioned variants, the head mass was 901 and 863 g, which is higher than the control (without treatment) by 166 and 128 g, or 22.3 and 17.4%.The highest yield of the ‘Batavia F1’ hybrid was obtained in 8 and 7 variants of the experiment – 33.5 and 31.9 t/ha, respectively, the yield increase was 5.2 and 3.6 t/ha, or 18.4 and 12.7%. In the hybrid ‘Orantes F1’ the highest yield (37.1 t/ha) was recorded when applying the “5 element” nanofertilizer in the 8th variant of the experiment, which is 6.6 t/ha higher than the control, or 21.6%. Compared to the ‘Batavia F1’ hybrid, the yield in this variant of the ‘Orantes F1’ hybrid was 3.6 t/ha, or 9.7%. The diameter and weight of the head, as well as the yield of the hybrid ‘Orantes F1’ were larger compared to the hybrid ‘Batavia F1’, due to its biological characteristics. A strong correlation (r = 0.97 and 0.96) was revealed between the yield and the diameter of the broccoli head in the ‘Batavia F1’ and ‘Orantes F1’ hybrids. The quality indicators of broccoli (dry matter, sum of sugars and vitamin C) in the ‘Orantes F1’ hybrid were higher compared to the ‘Batavia F1’ hybrid. A tendency towards a decrease in the concentration of nitrates in the heads of broccoli with an increase in the number of treatments with nanofertilizer “5 element” was revealed. The concentration of nitrates in the ‘Orantes F1’ hybrid was lower in all variants of the experiment in comparison with the ‘Batavia F1’ hybrid. The concentration of nitrates in the heads of broccoli in all variants of the experiment did not exceed TLV (400 mg/kg wet weight). Conclusions. With an increase in the number of foliar treatments with “5 element” nanofertilizer, the yield increased and the quality of broccoli hybrids improved. It was revealed that the yield of the ‘Orantes F1’ hybrid in all variants of the experiment was higher in comparison with the ‘Batavia F1’ hybrid. When applying nanofertilizer “5 element” in three stages – foliar feeding in the phase of 4–6 leaves + beginning of head formation + 10–12 days after the beginning of head formation – we got the highest yield of broccoli in the hybrid ‘Orantes F1’ – 37.1 t/ha, an increase over the control variant (without fertilizers) was 6.6 t/ha, or 21.6%. The ‘Batavia F1’ hybrid had a yield of 33.5 t/ha, an increase over the control variant – 5.2 t/ha, or 18.4%.

Purpose. To determine the efficiency of growing soybean varieties ‘Ustia’, ‘Cordoba’, ‘Estafeta’ depending on the influence of microfertilizers and growth regulators.Methods. Field, laboratory.Results. The article presents the results of studying the efficiency of growing different varieties of soybeans. It was found that in the cultivar ‘Ustia’ the best values of assimilation of photosynthetically active radiation were obtained for the applications of Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) with growth regulator Radostym, and Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) + in the flowering phase (0.25 l/ha) with two studied growth regulators. Similar patterns of the utilization rate of photosynthetic radiation were obtained for the ‘Cordoba’ and ‘Estafeta’ varieties. According to the indicators of the energy balance, the complex use of micronutrient fertilizers and growth regulators in any case pays off with an increase in the yield, which means it is energetically expedient. The maximum coefficients of energy efficiency in the ‘Ustia’, ‘Cordoba’ and ‘Estafeta’ varieties were obtained with the introduction of micronutrient fertilizers Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) + in the flowering phase (0.25 l/ha) and growth regulators Biosyl and Radostym. However, the use of growth regulators separately does not always provide an economic return on investment. In particular, in the variant without Yara Vita Mono Molitrak microfertilizer, the ‘Cordoba’ variety received 28 873 UAH/ha of net profit, while the introduction of growth regulators did not pay off, since there was no significant increase in the yield, and with the use of growth regulators Biosyl and Radostym net profit was within 28824–29179 UAH/ha. In general, the use of micronutrient fertilizer Yara Vita Mono Molitrak with growth regulators Radostim and Biosyl ensu­red guaranteed yield increase.Conclusions. When gro­wing ‘Ustia’, ‘Cordoba’ and ‘Estafeta’ varieties from an energy point of view, it is better to use Yara Vita Mono Molitrak microfertilizer in the budding phase (0.25 l/ha) with the growth regulator Radostym. Under these conditions, the obtained energy efficiency ratio was 3.24; 3.41 and 3.45, and the profit was 31503, 34072 and 34649 UAH/ha, respectively | Мета. Визначити ефективність вирощування сортів сої ‘Устя’, ‘Кордоба’, ‘Естафета’ залежно від впливу мікродобрив та регуляторів росту. Методи. Польові, лабораторні.Результати. У статті наведено результати вивчення ефективності вирощування різних сортів сої. Установлено, що в сорту ‘Устя’ кращі значення засвоєння фотосинтетично активної радіації отримано для варіантів застосування Yara Vita Моно Молітрак у фазі бутонізації (0,25 л/га) з регулятором росту Радостим, та Yara Vita Моно Молітрак у фазі бутонізації (0,25 л/га) + у фазі цвітіння (0,25 л/га) з обома досліджуваними регуляторами росту. Аналогічні закономірності коефіцієнту використання фотосинтетичної радіації були отримані й для сортів ‘Кордоба’ та ‘Естафета’. Згідно з показниками енергетичного балансу, комплексне застосування мікродобрив та регуляторів росту в будь-якому разі окупається приростом урожаю, а отже є енергетично доцільним. Максимальні коефіцієнти енергетичної ефективності в сортів ‘Устя’, ‘Кордоба’ та ‘Естафета’ отримано за внесення мікродобрива Yara Vita Моно Молітрак у фазі бутонізації (0,25 л/га) + у фазі цвітіння (0,25 л/га) та регуляторів росту: Біосил та Радостим. А от застосування регуляторів росту окремо не завжди дає змогу отримати економічну окупність. Зокрема, у варіанті без мікродобрива Yara Vita Моно Молітрак у сорту ‘Кордоба’ отримано 28873 грн/га чистого прибутку, тоді як внесення регуляторів росту не окупилось, оскільки не було значного приросту врожаю, а за застосування регуляторів росту Біосил та Радостим чистий прибуток був у межах 28824–29179 грн/га. Загалом застосування мікродобрива Yara Vita Моно Молітрак з регуляторами росту Радостим та Біосил забезпечувало гарантованого приросту врожаю.Висновки. За вирощування сої ‘Устя’, ‘Кордоба’ та ‘Естафета’ з енергетичного пог­ляду ліпше застосовувати мікродобриво Yara Vita Моно Молітрак у фазі бутонізації (0,25 л/га) з регулятором росту Радостим. За таких умов отримано коефіцієнт енергетичної ефективності становив 3,24; 3,41 і 3,45, а прибуток 31503, 34072 і 34649 грн/га відповідно.

Purpose. To determine the efficiency of growing soybean varieties ‘Ustia’, ‘Cordoba’, ‘Estafeta’ depending on the influence of microfertilizers and growth regulators. Methods. Field, laboratory. Results. The article presents the results of studying the efficiency of growing different varieties of soybeans. It was found that in the cultivar ‘Ustia’ the best values of assimilation of photosynthetically active radiation were obtained for the applications of Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) with growth regulator Radostym, and Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) + in the flowering phase (0.25 l/ha) with two studied growth regulators. Similar patterns of the utilization rate of photosynthetic radiation were obtained for the ‘Cordoba’ and ‘Estafeta’ varieties. According to the indicators of the energy balance, the complex use of micronutrient fertilizers and growth regulators in any case pays off with an increase in the yield, which means it is energetically expedient. The maximum coefficients of energy efficiency in the ‘Ustia’, ‘Cordoba’ and ‘Estafeta’ varieties were obtained with the introduction of micronutrient fertilizers Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) + in the flowering phase (0.25 l/ha) and growth regulators Biosyl and Radostym. However, the use of growth regulators separately does not always provide an economic return on investment. In particular, in the variant without Yara Vita Mono Molitrak microfertilizer, the ‘Cordoba’ variety received 28 873 UAH/ha of net profit, while the introduction of growth regulators did not pay off, since there was no significant increase in the yield, and with the use of growth regulators Biosyl and Radostym net profit was within 28824–29179 UAH/ha. In general, the use of micronutrient fertilizer Yara Vita Mono Molitrak with growth regulators Radostim and Biosyl ensu­red guaranteed yield increase. Conclusions. When gro­wing ‘Ustia’, ‘Cordoba’ and ‘Estafeta’ varieties from an energy point of view, it is better to use Yara Vita Mono Molitrak microfertilizer in the budding phase (0.25 l/ha) with the growth regulator Radostym. Under these conditions, the obtained energy efficiency ratio was 3.24; 3.41 and 3.45, and the profit was 31503, 34072 and 34649 UAH/ha, respectively

Purpose. To determine the efficiency of growing soybean varieties ‘Ustia’, ‘Cordoba’, ‘Estafeta’ depending on the influence of microfertilizers and growth regulators. Methods. Field, laboratory. Results. The article presents the results of studying the efficiency of growing different varieties of soybeans. It was found that in the cultivar ‘Ustia’ the best values of assimilation of photosynthetically active radiation were obtained for the applications of Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) with growth regulator Radostym, and Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) + in the flowering phase (0.25 l/ha) with two studied growth regulators. Similar patterns of the utilization rate of photosynthetic radiation were obtained for the ‘Cordoba’ and ‘Estafeta’ varieties. According to the indicators of the energy balance, the complex use of micronutrient fertilizers and growth regulators in any case pays off with an increase in the yield, which means it is energetically expedient. The maximum coefficients of energy efficiency in the ‘Ustia’, ‘Cordoba’ and ‘Estafeta’ varieties were obtained with the introduction of micronutrient fertilizers Yara Vita Mono Molitrak in the budding phase (0.25 l/ha) + in the flowering phase (0.25 l/ha) and growth regulators Biosyl and Radostym. However, the use of growth regulators separately does not always provide an economic return on investment. In particular, in the variant without Yara Vita Mono Molitrak microfertilizer, the ‘Cordoba’ variety received 28 873 UAH/ha of net profit, while the introduction of growth regulators did not pay off, since there was no significant increase in the yield, and with the use of growth regulators Biosyl and Radostym net profit was within 28824–29179 UAH/ha. In general, the use of micronutrient fertilizer Yara Vita Mono Molitrak with growth regulators Radostim and Biosyl ensu­red guaranteed yield increase. Conclusions. When gro­wing ‘Ustia’, ‘Cordoba’ and ‘Estafeta’ varieties from an energy point of view, it is better to use Yara Vita Mono Molitrak microfertilizer in the budding phase (0.25 l/ha) with the growth regulator Radostym. Under these conditions, the obtained energy efficiency ratio was 3.24; 3.41 and 3.45, and the profit was 31503, 34072 and 34649 UAH/ha, respectively

Purpose. Evaluate the introduced samples of soft winter wheat from the international nursery Common Bunt-Resistant Nursery (CBUNT-RES) of different ecological and geographical origin in the southern part of the Forest-Steppe zone of Ukraine according to a set of productivity indicators in order to define the most valuable samples and describe them.Methods. During 2016–2019 on the base of Ustymivka Experimental Station for Plant Production of the V. Ya. Yuriev Plant Production Institute of NAAS the authors studied, evaluated and described 75 new soft winter wheat samples of various eco-geographical origins by productivity traits. In the field and laboratory conditions, indicators of yield and productivi­ty were determined: 1000 grains weight, plant height and ear length, the number of spikelets and grains in the ear, weight of ear and grain and early-ripening.Results. Plant material with increased parameters of economic and biological characters was identified. During studies, it was found that ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘91-142A61/KATIA1//GRISET-4’, ‘SAULESKU#44/TR810200//GRISET-4’, ‘ATTILA/BABAX//PASTOR/4/…’ (IU067591) (Turkey), ‘INTENSIVNAYA//PBW343*2/TUKURU’, ‘SANZAR-8/KKTS’ (Mexico) were the high-yielding varieties. The samples of soft winter wheat as ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘INTENSIVNAYA//PBW343*2/…’ (IU067637) (Mexico), ‘ATAY/GALVEZ87/6/TAST/…’ (IU067587), ‘DE9/MERCAN-2’, ‘KRASNODAR/FRTL/6…’ (IU067595), ‘SAULESKU#44/TR810200//GRISET-4’, ‘GANSU-1/3/AUSGS50AT34/…’ (IU067598), ‘ORKINOS-1*2/3/AUS…’ (IU067608), ‘KAMBARA1/ZANDER-17’ , ‘TAM200/KAUZ/4/CHAM6//…’ (IU067612) (Turkey) contained a large grain weight (more than 5.0 g), plant productivity in these samples was rather high due to increased amount of grains and the thousand kernel weight.Conclusions. The introduced soft winter wheat samples of various eco-geographical origins were adapted to the Southern Forest-Steppe and can be recommended as a source material in breeding to increasing productive capacity. | Мета. Оцінити інтродуковані зразки пшениці м’якої озимої різного еколого-географічного походження з міжнародного розсадника Common Bunt-Resistant Nursery (CBUNT-RES) в умовах південної частини Лісостепу України за комплексом показників продуктивності для виділення найцінніших зразків та скласти їхній опис.Методи. Упродовж 2016–2019 рр. в умовах Устимівської дослідної станції рослинництва Інституту рослинництва ім. В. Я. Юр’єва НААН за ознаками продуктивності досліджено, оцінено та описано 75 нових зразків пшениці м’якої озимої різного еколого-географічного походження.  У польових і лабораторних умовах визначено такі показники врожайності й продуктивності, як маса 1000 зерен, висота рослин та довжина колоса, кількість колосків і зерен у колосі, маса колоса та зерна з нього, а також скоростиглість зразків.Результати. Виділено матеріал, який має підвищені параметри господарських та біологічних ознак. Зокрема, до високоврожайних зразків належать: ‘F08347G8’, ‘F00628G34-1’ (Румунія), ‘91-142A61/KATIA1//GRISET-4’, ‘SAULESKU#44/TR810200//GRISET-4’, ‘ATTILA/BABAX//PASTOR/4/…’ (IU067591) (Туреччина), ‘INTENSIVNAYA//PBW343*2/TUKURU’, ‘SANZAR-8/KKTS’ (Мексика). У зразків пшениці м’якої озимої ‘F08347G8’, ‘F00628G34-1’ (Румунія), ‘INTENSIVNAYA//PBW343*2/…’ (IU067637) (Мексика), ‘ATAY/GALVEZ87/6/TAST/…’ (IU067587), ‘DE9/MERCAN-2’, ‘KRASNODAR/ FRTL/6…’ (IU067595), ‘SAULESKU#44/TR810200//GRISET-4’, ‘KRASNODAR/FRTL/6…’ (IU067595), ‘GANSU-1/3/AUSGS50AT34/…’ (IU067598), ‘DE9/MERCAN-2’, ‘ORKINOS-1*2/3/AUS…’ (IU067608), ‘KAMBARA1/ZANDER-17’, ‘TAM200/KAUZ/4/CHAM6//…’ (IU067612) (Туреччина) маса зерна з рослини перевищувала 5,0 г, вони мають досить високі показники продуктивності рослини як завдяки підвищеній озерненості, так і масі 1000 зерен.Висновки. Інтродуковані зразки пшениці м’якої озимої різного еколого-географічного походження, виділені за комплексом цінних ознак, можна рекомендувати як вихідний матеріал у селекції на підвищення продуктивного потенціалу культури в умовах Південного Лісостепу України.

Purpose. Evaluate the introduced samples of soft winter wheat from the international nursery Common Bunt-Resistant Nursery (CBUNT-RES) of different ecological and geographical origin in the southern part of the Forest-Steppe zone of Ukraine according to a set of productivity indicators in order to define the most valuable samples and describe them. Methods. During 2016–2019 on the base of Ustymivka Experimental Station for Plant Production of the V. Ya. Yuriev Plant Production Institute of NAAS the authors studied, evaluated and described 75 new soft winter wheat samples of various eco-geographical origins by productivity traits. In the field and laboratory conditions, indicators of yield and productivi­ty were determined: 1000 grains weight, plant height and ear length, the number of spikelets and grains in the ear, weight of ear and grain and early-ripening. Results. Plant material with increased parameters of economic and biological characters was identified. During studies, it was found that ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘91-142A61/KATIA1//GRISET-4’, ‘SAULESKU#44/TR810200//GRISET-4’, ‘ATTILA/BABAX//PASTOR/4/…’ (IU067591) (Turkey), ‘INTENSIVNAYA//PBW343*2/TUKURU’, ‘SANZAR-8/KKTS’ (Mexico) were the high-yielding varieties. The samples of soft winter wheat as ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘INTENSIVNAYA//PBW343*2/…’ (IU067637) (Mexico), ‘ATAY/GALVEZ87/6/TAST/…’ (IU067587), ‘DE9/MERCAN-2’, ‘KRASNODAR/FRTL/6…’ (IU067595), ‘SAULESKU#44/TR810200//GRISET-4’, ‘GANSU-1/3/AUSGS50AT34/…’ (IU067598), ‘ORKINOS-1*2/3/AUS…’ (IU067608), ‘KAMBARA1/ZANDER-17’ , ‘TAM200/KAUZ/4/CHAM6//…’ (IU067612) (Turkey) contained a large grain weight (more than 5.0 g), plant productivity in these samples was rather high due to increased amount of grains and the thousand kernel weight. Conclusions. The introduced soft winter wheat samples of various eco-geographical origins were adapted to the Southern Forest-Steppe and can be recommended as a source material in breeding to increasing productive capacity.

Purpose. Evaluate the introduced samples of soft winter wheat from the international nursery Common Bunt-Resistant Nursery (CBUNT-RES) of different ecological and geographical origin in the southern part of the Forest-Steppe zone of Ukraine according to a set of productivity indicators in order to define the most valuable samples and describe them. Methods. During 2016–2019 on the base of Ustymivka Experimental Station for Plant Production of the V. Ya. Yuriev Plant Production Institute of NAAS the authors studied, evaluated and described 75 new soft winter wheat samples of various eco-geographical origins by productivity traits. In the field and laboratory conditions, indicators of yield and productivi­ty were determined: 1000 grains weight, plant height and ear length, the number of spikelets and grains in the ear, weight of ear and grain and early-ripening. Results. Plant material with increased parameters of economic and biological characters was identified. During studies, it was found that ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘91-142A61/KATIA1//GRISET-4’, ‘SAULESKU#44/TR810200//GRISET-4’, ‘ATTILA/BABAX//PASTOR/4/…’ (IU067591) (Turkey), ‘INTENSIVNAYA//PBW343*2/TUKURU’, ‘SANZAR-8/KKTS’ (Mexico) were the high-yielding varieties. The samples of soft winter wheat as ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘INTENSIVNAYA//PBW343*2/…’ (IU067637) (Mexico), ‘ATAY/GALVEZ87/6/TAST/…’ (IU067587), ‘DE9/MERCAN-2’, ‘KRASNODAR/FRTL/6…’ (IU067595), ‘SAULESKU#44/TR810200//GRISET-4’, ‘GANSU-1/3/AUSGS50AT34/…’ (IU067598), ‘ORKINOS-1*2/3/AUS…’ (IU067608), ‘KAMBARA1/ZANDER-17’ , ‘TAM200/KAUZ/4/CHAM6//…’ (IU067612) (Turkey) contained a large grain weight (more than 5.0 g), plant productivity in these samples was rather high due to increased amount of grains and the thousand kernel weight. Conclusions. The introduced soft winter wheat samples of various eco-geographical origins were adapted to the Southern Forest-Steppe and can be recommended as a source material in breeding to increasing productive capacity.

Purpose. Morphological and organoleptic evaluation of oyster mushroom strains for selection of highly productive cultivars assortment with valuable consumer properties for winter and summer cultivation and suitability for sale in fresh or processed form were investigated.Methods. The scheme of the experiment included six strains of oyster mushrooms belonging to two species: Pleurotus ostreatus (5 strains: 2301, Z, 2316, 2456, 431) and P. pulmonarius (2314). Laboratory, laboratory-industrial and statistical methods are used.Results. The dynamics of agrochemical parameters of substrates obtained by the method of aerobic fermentation in the high layer proved their optimality according to the main criteria and compliance to regulatory documentation. The biological efficiency of the strains was in the range of 40–78.9%. The highest weight of clusters was found in the “summer” strain 431 (430.7 g). The highest weight of the fruiting body was determined for the strain 2301 (15.2 g), the lowest – for the 2456 (3.3 g).Conclusions. The values of biological efficiency of strains was determined. The highest average values for the first flush of fruiting had strains 2316 (78.9%) from the “winter” group (A) and 431 (78.4%) from the “summer” group  (B). According to the results of statistical analysis, significant differences were revealed between the studied strains in the main morphological indicators of clusters. It was found that the mass of clusters of «winter» cultivars is much less dependent on the strain type (220.8–273.4 g) than that of «summer» cultivars (83.4–430.7 g). The index of the asymmetry coefficient of the cluster, which can be useful for calculating the required container sizes was proposed. It was determined that the morphological features of fruiting bodies had significant differences in all studied parameters.  In particular, the A group strains differed in size and weight. It was proposed the index of weight loss of the crop coefficient, which shows the ratio between the cap and stipe of the fruiting body and make it possible to predict the amount of mushroom raw material that will be sold in the form of caps, and the amount of raw material that can be processed into minced mushroom, powder and others. The best coefficient was obtained for the fruiting bodies of strain 2314 (0.87), and the worst – for 2456 (0.59), which, accordingly, is not recommended for sale by individual fruiting bodies. It was found that the fruiting bodies of high-yielding strains 2316 and 431 had a number of organoleptic defects. | Мета. Провести морфологічну та органолептичну оцінку штамів гливи для добору асортименту високопродуктивних і цінних за споживчими властивостями культиварів для зимового й літнього культивування та придатності до реалізації у свіжому або переробленому вигляді.Методи. Схема досліду включає 6 штамів гливи, що належать до двох видів: Pleurotus ostreatus (5 штамів: 2301, Z, 2316, 2456, 431) і Pleurotus pulmonarius (2314). Використано лабораторний, лабораторно-виробничий та статистичний методи досліджень.Результати. Динаміка агрохімічних показників субстратів, отриманих методом аеробної ферментації у високому шарі, засвідчила їхню оптимальність за основними критеріями та відповідність нормативній документації. Біологічна ефективність штамів знаходилася в інтервалі 40–78,9%. Найбільшу масу зростків установлено в «літнього» штаму 431 (430,7 г). Найбільшу масу плодового тіла визначено для штаму 2301 (15,2 г), найменшу – для 2456 (3,3 г).Висновки. Визначено показники біологічної ефективності штамів. Найвищі середні значення за першою хвилею плодоношення мали штами 2316 (78,9%) із групи «зимових» (А) і 431 (78,4%) із групи «літніх» (В). За результатами статистичного аналізу встановлено суттєві відмінності між досліджуваними штамами за основними морфологічними показниками зростків. Виявлено, що маса зростків «зимових» культиварів значно менше залежить від штамової приналежності (220,8–273,4 г), ніж «літніх» (83,4–430,7 г). Запропоновано показник коефіцієнт асиметрії зростка, який може бути корисним для розрахунку необхідних розмірів тари. Виявлено, що морфологічні ознаки плодових тіл мали суттєві відмінності за всіма дослідженими параметрами. Зокрема, штами групи А відрізнялися більшими розмірами й масою. Запропоновано показник – коефіцієнт утрати маси врожаю, який показує співвідношення між шапинкою й ніжкою плодового тіла та дає змогу спрогнозувати кількість грибної сировини, яку буде реалізовано у вигляді шапинок, та кількість сировини, яку можна переробити у грибний фарш, порошок та ін. Найкращий коефіцієнт отримано для плодових тіл штаму 2314 (0,87), а найгірший – 2456 (0,59), який, відповідно, не рекомендуємо до реалізації окремими плодовими тілами. Установлено, що плодові тіла високопродуктивних штамів 2316 і 431 мали низку органолептичних недоліків.

Purpose. Morphological and organoleptic evaluation of oyster mushroom strains for selection of highly productive cultivars assortment with valuable consumer properties for winter and summer cultivation and suitability for sale in fresh or processed form were investigated. Methods. The scheme of the experiment included six strains of oyster mushrooms belonging to two species: Pleurotus ostreatus (5 strains: 2301, Z, 2316, 2456, 431) and P. pulmonarius (2314). Laboratory, laboratory-industrial and statistical methods are used. Results. The dynamics of agrochemical parameters of substrates obtained by the method of aerobic fermentation in the high layer proved their optimality according to the main criteria and compliance to regulatory documentation. The biological efficiency of the strains was in the range of 40–78.9%. The highest weight of clusters was found in the “summer” strain 431 (430.7 g). The highest weight of the fruiting body was determined for the strain 2301 (15.2 g), the lowest – for the 2456 (3.3 g). Conclusions. The values of biological efficiency of strains was determined. The highest average values for the first flush of fruiting had strains 2316 (78.9%) from the “winter” group (A) and 431 (78.4%) from the “summer” group  (B). According to the results of statistical analysis, significant differences were revealed between the studied strains in the main morphological indicators of clusters. It was found that the mass of clusters of «winter» cultivars is much less dependent on the strain type (220.8–273.4 g) than that of «summer» cultivars (83.4–430.7 g). The index of the asymmetry coefficient of the cluster, which can be useful for calculating the required container sizes was proposed. It was determined that the morphological features of fruiting bodies had significant differences in all studied parameters.  In particular, the A group strains differed in size and weight. It was proposed the index of weight loss of the crop coefficient, which shows the ratio between the cap and stipe of the fruiting body and make it possible to predict the amount of mushroom raw material that will be sold in the form of caps, and the amount of raw material that can be processed into minced mushroom, powder and others. The best coefficient was obtained for the fruiting bodies of strain 2314 (0.87), and the worst – for 2456 (0.59), which, accordingly, is not recommended for sale by individual fruiting bodies. It was found that the fruiting bodies of high-yielding strains 2316 and 431 had a number of organoleptic defects.

Purpose. Morphological and organoleptic evaluation of oyster mushroom strains for selection of highly productive cultivars assortment with valuable consumer properties for winter and summer cultivation and suitability for sale in fresh or processed form were investigated. Methods. The scheme of the experiment included six strains of oyster mushrooms belonging to two species: Pleurotus ostreatus (5 strains: 2301, Z, 2316, 2456, 431) and P. pulmonarius (2314). Laboratory, laboratory-industrial and statistical methods are used. Results. The dynamics of agrochemical parameters of substrates obtained by the method of aerobic fermentation in the high layer proved their optimality according to the main criteria and compliance to regulatory documentation. The biological efficiency of the strains was in the range of 40–78.9%. The highest weight of clusters was found in the “summer” strain 431 (430.7 g). The highest weight of the fruiting body was determined for the strain 2301 (15.2 g), the lowest – for the 2456 (3.3 g). Conclusions. The values of biological efficiency of strains was determined. The highest average values for the first flush of fruiting had strains 2316 (78.9%) from the “winter” group (A) and 431 (78.4%) from the “summer” group (B). According to the results of statistical analysis, significant differences were revealed between the studied strains in the main morphological indicators of clusters. It was found that the mass of clusters of «winter» cultivars is much less dependent on the strain type (220.8–273.4 g) than that of «summer» cultivars (83.4–430.7 g). The index of the asymmetry coefficient of the cluster, which can be useful for calculating the required container sizes was proposed. It was determined that the morphological features of fruiting bodies had significant differences in all studied parameters. In particular, the A group strains differed in size and weight. It was proposed the index of weight loss of the crop coefficient, which shows the ratio between the cap and stipe of the fruiting body and make it possible to predict the amount of mushroom raw material that will be sold in the form of caps, and the amount of raw material that can be processed into minced mushroom, powder and others. The best coefficient was obtained for the fruiting bodies of strain 2314 (0.87), and the worst – for 2456 (0.59), which, accordingly, is not recommended for sale by individual fruiting bodies. It was found that the fruiting bodies of high-yielding strains 2316 and 431 had a number of organoleptic defects.

Purpose. To evaluate Tartary buckwheat variety ‘Kalyna’ according to morphological, productive and quality indicators in the conditions of the Research Center “State Agra­rian and Engineering University in Podilia”. Methods. The studies were carried out in the field crop rotation of the Research Center “Podillia” of the State Agrarian and Engi­neering University in Podilia (SAEU) in 2015–2020. The soil cover of the experimental field is represented mainly by low-humus, weakly leached soils. Experiment setting, material evaluation, analysis of plants, yield and grain quality were carried out in accordance with the generally accepted methods of state variety testing. An assessment of the Tartary buckwheat variety ‘Kalyna’ was carried out in comparison with the common buckwheat variety ‘Victoriia’. Results. The growing season duration of Tartary buckwheat variety ‘Kalyna’ was 87 days (38 days vegetative and 49 days gene­rative). In the studied variety, a larger number of the 1st order branches up to 5.5 pcs was observed. The number of leaves on the plant was 22–28 pcs./plants. In the studied variety, more than 50% of the grains were located on the main stem and the first order branches, while in common buckwheat this indicator was only 30%. Productivity was 2,28 t/ha, the number of grains in inflorescences was 4,4 grains, in common buckwheat – 0,72 t/ha, and 2.2 grains/inflorescence, respectively. The thousand kernel weight in Tartary buckwheat variety ‘Kalyna’ is 10.2 g less than in common buckwheat variety ‘Victoriia’ and is 17.4 g, with hulls – 19.5%, which is 3.2% less then in common buckwheat variety.Assessment of Tartary buckwheat variety ‘Kalyna’ for resistance to the effects of bio- and abiotic factors indicates that it was at the level of common buckwheat variety ‘Victoriia’.In terms of resistance to abiotic factors, ‘Kalyna’ variety is characterized by high resistance to spontaneous fallof grain (5 scores) and has simultaneous grain ripening (5 scores). Conclusions.The morphological and economic-biological indicators of Tartary buckwheat variety ‘Kalyna’ are significantly higher than the common buckwheat varie­ty ‘Victoriia’. It is recommended to grow Tartary buckwheat variety ‘Kalyna’ as a valuable cereal crop in the Western Forest-Steppe zone. | Мета. Оцінити сорт гречки татарської ‘Калина’ за морфологічними, урожайними та якісними показниками в умовах Лісостепу Західного.Методи. Дослідження проводили в польовій сівозміні Науково-дослідного центру «Поділля» Подільського державного аграрно-технічного університету (НДЦ «Поділля» ПДАТУ) упродовж 2015–2020 рр. Ґрунтовий покрив дослідного поля – чорнозем малогумусний слабковилугуваний. Закладання дослідів, оцінку матеріалу, аналіз рослин, урожаю та якості зерна проводили відповідно до методик державного сортовипробування. Сорт гречки татарської ‘Калина’ оцінювали порівняно із сортом гречки їстівної ‘Вікторія’.Результати. Тривалість вегетаційного періоду гречки татарської сорту ‘Калина’ становила 87 діб (вегетативний період – 38 діб, генеративний – 49 діб). У досліджуваного сорту спостерігали більшу кількість гілок 1-го порядку (до 5,5 шт.) та листків на рослині (22–28 шт.). Понад 50% зерен розміщено на головному стеблі й гілках першого порядку, тоді як у гречки їстівної – лише 30%. Урожайність сорту ‘Калина’ становить 2,28 т/га, озерненість суцвіть – 4,4 зерен, що перевищує показники гречки їстівної на 0,72 т/га і 2,2 зерен/суцвіття відповідно. Маса 1000 зерен у сорту гречки татарської ‘Калина’ на 10,2 г менше сорту гречки їстівної ‘Вікторія’ і становить 17,4 г, плівчастість – 19,5%, що менше на 3,2%. Оцінка сорту гречки татарської ‘Калина’ на стійкість до впливу біо- та абіотичних чинників свідчить про те, що вона була на рівні сорту гречки їстівної ‘Вікторія’. За стійкістю до абіотичних чинників сорт ‘Калина’ характеризується вищою стійкістю до осипання плодів (5 балів) і має високу дружність достигання плодів (5 балів).Висновки. Морфологічні та господарсько-біологічні показники сорту гречки татарської ‘Калина’ суттєво вищі сорту гречки їстівної ‘Вікторія’. В умовах Лісостепу Західного рекомендується вирощувати сорт гречки татарської ‘Калина’ як цінну круп’яну культуру.

Purpose. To evaluate Tartary buckwheat variety ‘Kalyna’ according to morphological, productive and quality indicators in the conditions of the Research Center “State Agra­rian and Engineering University in Podilia”. Methods. The studies were carried out in the field crop rotation of the Research Center “Podillia” of the State Agrarian and Engi­neering University in Podilia (SAEU) in 2015–2020. The soil cover of the experimental field is represented mainly by low-humus, weakly leached soils. Experiment setting, material evaluation, analysis of plants, yield and grain quality were carried out in accordance with the generally accepted methods of state variety testing. An assessment of the Tartary buckwheat variety ‘Kalyna’ was carried out in comparison with the common buckwheat variety ‘Victoriia’. Results. The growing season duration of Tartary buckwheat variety ‘Kalyna’ was 87 days (38 days vegetative and 49 days gene­rative). In the studied variety, a larger number of the 1st order branches up to 5.5 pcs was observed. The number of leaves on the plant was 22–28 pcs./plants. In the studied variety, more than 50% of the grains were located on the main stem and the first order branches, while in common buckwheat this indicator was only 30%. Productivity was 2,28 t/ha, the number of grains in inflorescences was 4,4 grains, in common buckwheat – 0,72 t/ha, and 2.2 grains/inflorescence, respectively. The thousand kernel weight in Tartary buckwheat variety ‘Kalyna’ is 10.2 g less than in common buckwheat variety ‘Victoriia’ and is 17.4 g, with hulls – 19.5%, which is 3.2% less then in common buckwheat variety.Assessment of Tartary buckwheat variety ‘Kalyna’ for resistance to the effects of bio- and abiotic factors indicates that it was at the level of common buckwheat variety ‘Victoriia’.In terms of resistance to abiotic factors, ‘Kalyna’ variety is characterized by high resistance to spontaneous fallof grain (5 scores) and has simultaneous grain ripening (5 scores). Conclusions.The morphological and economic-biological indicators of Tartary buckwheat variety ‘Kalyna’ are significantly higher than the common buckwheat varie­ty ‘Victoriia’. It is recommended to grow Tartary buckwheat variety ‘Kalyna’ as a valuable cereal crop in the Western Forest-Steppe zone.

Purpose. To evaluate Tartary buckwheat variety ‘Kalyna’ according to morphological, productive and quality indicators in the conditions of the Research Center “State Agra­rian and Engineering University in Podilia”. Methods. The studies were carried out in the field crop rotation of the Research Center “Podillia” of the State Agrarian and Engi­neering University in Podilia (SAEU) in 2015–2020. The soil cover of the experimental field is represented mainly by low-humus, weakly leached soils. Experiment setting, material evaluation, analysis of plants, yield and grain quality were carried out in accordance with the generally accepted methods of state variety testing. An assessment of the Tartary buckwheat variety ‘Kalyna’ was carried out in comparison with the common buckwheat variety ‘Victoriia’. Results. The growing season duration of Tartary buckwheat variety ‘Kalyna’ was 87 days (38 days vegetative and 49 days gene­rative). In the studied variety, a larger number of the 1st order branches up to 5.5 pcs was observed. The number of leaves on the plant was 22–28 pcs./plants. In the studied variety, more than 50% of the grains were located on the main stem and the first order branches, while in common buckwheat this indicator was only 30%. Productivity was 2,28 t/ha, the number of grains in inflorescences was 4,4 grains, in common buckwheat – 0,72 t/ha, and 2.2 grains/inflorescence, respectively. The thousand kernel weight in Tartary buckwheat variety ‘Kalyna’ is 10.2 g less than in common buckwheat variety ‘Victoriia’ and is 17.4 g, with hulls – 19.5%, which is 3.2% less then in common buckwheat variety.Assessment of Tartary buckwheat variety ‘Kalyna’ for resistance to the effects of bio- and abiotic factors indicates that it was at the level of common buckwheat variety ‘Victoriia’.In terms of resistance to abiotic factors, ‘Kalyna’ variety is characterized by high resistance to spontaneous fallof grain (5 scores) and has simultaneous grain ripening (5 scores). Conclusions.The morphological and economic-biological indicators of Tartary buckwheat variety ‘Kalyna’ are significantly higher than the common buckwheat varie­ty ‘Victoriia’. It is recommended to grow Tartary buckwheat variety ‘Kalyna’ as a valuable cereal crop in the Western Forest-Steppe zone.

Purpose. The analysis of allelic status of such key genes of carotenogenesis as gene of lycopene-ε-cyclase (lcyε) and gene of β-carotene hydroxylase (crtRB1) for DNA-markers in domestic maize lines and their hybrids. Methods. DNA isolation, PCR, gel electrophoresis. Results. Allelic status of key genes of carotenogenesis was investigated in eight maize inbred lines and their single crosses. Molecular genetic polymorphism in the studied sample of maize lines and hyb­rids has been detected in gene of β-carotene hydroxylase for marker crtRB1-3’TE. For this gene, codominant character of inheritance of alleles of parental lines in single crosses was confirmed. For markers of gene of lycopene-ε-cyclase lcyε-3’INDL and lcyε-SNP216, polymorphism in the group of investigated lines and hybrids has not been identified, genotypes included only one variant of alleles for each marker. For lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’, the decrease of the activi­ty of β-carotene hydroxylase owing to the mutation of gene crtRB1 under the influence of transposone element at the 3’-end, the inhibition of β-carotene transition into β-cryptoxanthin can be expected, that allows to predict β-carotene accumulation in grain. Conclusions. The study of allelic status of carotenegenesis gene of lycopene-ε-cyclase in maize showed no polymorphism for markers lcyε-3’INDL and lcyε-SNP216 in eight inbred lines and their single crosses, along with this, for marker lcyε-3’INDL in genomes of all studied samples the allele was identified to be favorable for the accumulation of β-carotene. For marker crtRB1-3’TE of gene of β-carotene hydroxylase, the studied breeding material was polymorphic. Allele of crtRB1 being favorable for the accumulation of β-carotene was identified in lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’. Single crosses inherit maternal and paternal alleles of gene of β-carotene hydroxylase codominantly. | Цель. Анализ аллельного состояния ключевых генов каротиногенеза – гена ликопин-ε-циклазы (lcyε) и гена β-каротингидроксилазы (crtRB1) по ДНК-маркерам у линий кукурузы отечественной селекции и их гибридов. Методы. Выделение ДНК, полимеразная цепная реакция, гель-электрофорез. Результаты. Исследовано аллельное состояние ключевых генов каротиногенеза у восьми инбредных линий кукурузы и их простых гибридов. Молекулярно-генетический полиморфизм в исследованной выборке линий и гибридов был выявлен по гену β-каротингидроксилазы для маркера crtRB1-3’TE. Для этого гена подтвержден кодоминантный характер наследования аллелей родительских линий в простых гибридах. По маркерам гена ликопин-ε-циклазы lcyε-3’INDL и lcyε-SNP216 среди исследованных линий и гибридов полиморфизма не было выявлено, изученные генотипы имели по одному варианту аллелей каждого маркера. Для линий ‘ДК253ЗСЗМ’, ‘ДК633/266зС,зМ’, ‘ДК366зС,зМ’ и гибридов ‘ДК296С×ДК253ЗСЗМ’, ‘ДК272С×ДК633/266зС,зМ’ и ‘ДК231С×ДК366зС,зМ’ следует ожидать снижения активнос­ти фермента β-каротингидроксилазы в результате мутации гена crtRB1 под воздействием транспозонового элемента на 3’-конце, ингибирования перехода β-каротина в β-криптоксантин, что позволяет прогнозировать накоп­ление β-каротина в зерне. Выводы. В результате проведенного исследования аллельного состояния генов каротиногенеза у кукурузы было установлено отсутствие полиморфизма по маркерам lcyε-3’INDL и lcyε-SNP216 гена ликопин-ε-циклазы у восьми инбредных линий и их простых гибридов, причем по маркеру lcyε-3’INDL в геномах всех изученных образцов содержится благоприятный для накопления β-каротина аллель. По маркеру crtRB1-3’TE гена β-каротингидроксилазы изученный селекционный материал оказался полиморфным. Благоприятный для накопления β-каротина аллель гена crtRB1 идентифицирован у линий ‘ДК253ЗСЗМ’, ‘ДК633/266зС,зМ’, ‘ДК366зС,зМ’ и гибридов ‘ДК296С×ДК253ЗСЗМ’, ‘ДК272С×ДК633/266зС,зМ’ и ‘ДК231С×ДК366зС,зМ’. Простые гибриды кодоминантно наследуют аллели гена β-каротингидроксилазы материнской и отцовской линий. | Мета. Аналіз алельного стану ключових генів каротиногенезу – гена лікопін-ε-циклази (lcyε) та гена β-каротингідроксилази (crtRB1) за ДНК-маркерами lcyε-3’INDL, lcyε-SNP216 та crtRB1-3’ТЕ у ліній кукурудзи вітчизняної селекції та їхніх гібридів. Методи. Виділення ДНК, полімеразна ланцюгова реакція, гель-електрофорез. Результати. Досліджено алельний стан ключових генів каротиногенезу за ДНК-маркерами у восьми інбредних ліній кукурудзи та їхніх простих гібридів. Молекулярно-генетичний поліморфізм у дослідженій добірці ліній і гібридів був виявлений за геном β-каротингідроксилази для маркера crtRB1-3’TE. Для цього гена підтверджено кодомінантний характер успадкування алелей батьківських ліній у простих гібридах. За маркерами гена лікопін-ε-циклази lcyε-3’INDL та lcyε-SNP216 серед досліджених ліній і гібридів поліморфізму не виявлено, вивчені генотипи містили лише по одному варіанту алелей кожного маркера. Для ліній ‘ДК253ЗСЗМ’, ‘ДК633/266зС,зМ’, ‘ДК366зС,зМ’ та гібридів ‘ДК296С×ДК253ЗСЗМ’, ‘ДК272С×ДК633/266зС,зМ’ і ‘ДК231С×ДК366зС,зМ’ слід очікувати зниження активності ферменту β-каротингідроксилази внаслідок мутації гена crtRB1 у разі впливу транспозонового елемента на 3’-кінці, а також інгібування переходу β-каротину в β-криптоксантин, що дає змогу прогнозувати накопичення β-каротину в зерні. Висновки. Внаслідок проведеного дослідження алельного стану генів каротиногенезу в кукурудзи було встановлено відсутність поліморфізму за маркерами lcyε-3’INDL та lcyε-SNP216 гена лікопін-ε-циклази у восьми інбредних ліній та їхніх простих гібридів, причому за маркером lcyε-3’INDL у геномах усіх досліджених зразків міститься сприятливий для накопичення β-каротину алель. За маркером crtRB1-3’TE гена β-каротингідроксилази досліджений селекційний матеріал виявився поліморфним. Сприятливий для накопичення β-каротину алель гена crtRB1 ідентифіковано в лініях ‘ДК253ЗСЗМ’, ‘ДК633/266зС,зМ’, ‘ДК366зС,зМ та гібридах ‘ДК296С×ДК253ЗСЗМ’, ‘ДК272С×ДК633/266зС,зМ’ і ‘ДК231С×ДК366зС,зМ’. Прості гібриди кодомінантно успадковують алелі гена β-каротингідроксилази материнської та батьківської ліній.

Purpose. The analysis of allelic status of such key genes of carotenogenesis as gene of lycopene-ε-cyclase (lcyε) and gene of β-carotene hydroxylase (crtRB1) for DNA-markers in domestic maize lines and their hybrids. Methods. DNA isolation, PCR, gel electrophoresis. Results. Allelic status of key genes of carotenogenesis was investigated in eight maize inbred lines and their single crosses. Molecular genetic polymorphism in the studied sample of maize lines and hyb­rids has been detected in gene of β-carotene hydroxylase for marker crtRB1-3’TE. For this gene, codominant character of inheritance of alleles of parental lines in single crosses was confirmed. For markers of gene of lycopene-ε-cyclase lcyε-3’INDL and lcyε-SNP216, polymorphism in the group of investigated lines and hybrids has not been identified, genotypes included only one variant of alleles for each marker. For lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’, the decrease of the activi­ty of β-carotene hydroxylase owing to the mutation of gene crtRB1 under the influence of transposone element at the 3’-end, the inhibition of β-carotene transition into β-cryptoxanthin can be expected, that allows to predict β-carotene accumulation in grain. Conclusions. The study of allelic status of carotenegenesis gene of lycopene-ε-cyclase in maize showed no polymorphism for markers lcyε-3’INDL and lcyε-SNP216 in eight inbred lines and their single crosses, along with this, for marker lcyε-3’INDL in genomes of all studied samples the allele was identified to be favorable for the accumulation of β-carotene. For marker crtRB1-3’TE of gene of β-carotene hydroxylase, the studied breeding material was polymorphic. Allele of crtRB1 being favorable for the accumulation of β-carotene was identified in lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’. Single crosses inherit maternal and paternal alleles of gene of β-carotene hydroxylase codominantly.

Purpose. The analysis of allelic status of such key genes of carotenogenesis as gene of lycopene-ε-cyclase (lcyε) and gene of β-carotene hydroxylase (crtRB1) for DNA-markers in domestic maize lines and their hybrids. Methods. DNA isolation, PCR, gel electrophoresis. Results. Allelic status of key genes of carotenogenesis was investigated in eight maize inbred lines and their single crosses. Molecular genetic polymorphism in the studied sample of maize lines and hyb­rids has been detected in gene of β-carotene hydroxylase for marker crtRB1-3’TE. For this gene, codominant character of inheritance of alleles of parental lines in single crosses was confirmed. For markers of gene of lycopene-ε-cyclase lcyε-3’INDL and lcyε-SNP216, polymorphism in the group of investigated lines and hybrids has not been identified, genotypes included only one variant of alleles for each marker. For lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’, the decrease of the activi­ty of β-carotene hydroxylase owing to the mutation of gene crtRB1 under the influence of transposone element at the 3’-end, the inhibition of β-carotene transition into β-cryptoxanthin can be expected, that allows to predict β-carotene accumulation in grain. Conclusions. The study of allelic status of carotenegenesis gene of lycopene-ε-cyclase in maize showed no polymorphism for markers lcyε-3’INDL and lcyε-SNP216 in eight inbred lines and their single crosses, along with this, for marker lcyε-3’INDL in genomes of all studied samples the allele was identified to be favorable for the accumulation of β-carotene. For marker crtRB1-3’TE of gene of β-carotene hydroxylase, the studied breeding material was polymorphic. Allele of crtRB1 being favorable for the accumulation of β-carotene was identified in lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’. Single crosses inherit maternal and paternal alleles of gene of β-carotene hydroxylase codominantly.

Purpose. To study the dynamics of soft winter wheat lectin activity in caryopsis and at the early stages of ontogenesis of varieties developed by Poltava State Agrarian Academy as well as investigate varietal differences depending on dynamic pH medium. Methods. Laboratory analysis, method of hema­gglutination reaction, analytical approach, statistical evaluation. Results. A change in lectin activity was determined in samples of 14 soft winter wheat varieties in caryopsis and at the early stages of ontogenesis (on the third, seventh, tenth and twelfth day of seedlings emergence). High lectin activity was observed in samples of seven and ten days seedlings. This could be evidence of active participation of proteins in physio­logical and biochemical processes at the time of emerging wheat tillering node that can be important in forming adaptive reactions to stress. Wheat lectin activity variation for рН ranging between 4,0 and 8,0 that depended on a variety was defined for the first time. Conclusions. According to hema­gglutination ability of wheat lectins, all varieties were divided into three groups: of high ability – 4 varieties, middle – 9 varieties, low – 1 variety. Varietal variability for the investigated trait can be used in breeding programs as marker trait. In most varieties variation of hemagglutination was observed at dilutions ranging between 1:2 and 1:256. Availability of two lectin agglutination peaks in acidic and alkaline media for seedlings was fixed. | Цель. Изучить динамику активности лектинов пшеницы мягкой озимой в зерновках на ранних этапах онтогенеза сортов селекции Полтавской государственной аграрной академии, а также исследовать сортовые различия в зависимости от динамической рН среды. Методы. Лабораторный, метод реакции гемагглютинации (РГА), аналитический, статистический. Результаты. Определено изменение активности лектинов в образцах пшеницы мягкой озимой 14 сортов в зерновках и на ранних этапах онтогенеза (на третьи, седьмые, десятые и двенадцатые сутки прорастания). Высокая активность лектинов наблюдалась в образцах семи- и десятисуточных проростков. Это свидетельствует об активном включении этих белков в физимолого-биохимические процессы при образовании узла кущения пшеницы, что может иметь значение для формирования адаптивных реакций к действию стресса. Впервые установлены колебания активности лектинов пшеницы в диапазоне рН от 4,0 до 8,0 в зависимости от сорта. Выводы. По гемагглютинирующей способности лектинов пшеницы сорта были разделены на три группы: с высокой – 4 сорта; со средней – 9; с низкой – 1 сорт. Сортовая вариабельность по исследуемому признаку может быть использована в селекционных программах как маркерный признак. У большинства сортов наблюдались колебания гемагглютинации при разведениях от 1:2 до 1:256. Установлено наличие двух максимумов агглютинации лектинов в кислой и щелочной среде для проростков. | Мета. Вивчити динаміку активності лектинів пшениці м’якої озимої у зернівках та на ранніх етапах онтогенезу сортів селекції Полтавської державної аграрної академії, а також дослідити сортові відмінності залежно від динамічного рН середовища. Методи. Лабораторний, метод реакції гемаглютинації (РГА), аналітичний, статистичний. Результати. Визначено зміну активності лектинів у зразках пшениці м’якої озимої 14 сортів у зернівках та на ранніх етапах онтогенезу (на третю, сьому, десяту та дванадцяту добу проростання). Висока активність лектинів спостерігалася у зразках семи- і десятидобових проростків. Це свідчить про активне включення цих білків у фізіолого-біохімічні процеси під час утворення вузла кущіння пшениці, що може мати значення для формування адаптивних реакцій до дії стресу. Вперше встановлено коливання активності лектинів пшениці у діапазоні рН від 4,0 до 8,0 залежно від сорту. Висновки. За гемаглютинувальною здатністю лектинів пшениці сорти були розподілені на три групи: з високою – 4 сорти; з середньою – 9; з низькою – 1 сорт. Сортова варіабельність за досліджуваною ознакою може бути використана в селекційних програмах як маркерна ознака. У більшості сортів спостерігалися коливання гемаглютинації при розведеннях від 1:2 до 1:256. Встановлено наявність двох максимумів аглютинації лектинів у кислому й лужному середовищі для проростків.

Purpose. To study the dynamics of soft winter wheat lectin activity in caryopsis and at the early stages of ontogenesis of varieties developed by Poltava State Agrarian Academy as well as investigate varietal differences depending on dynamic pH medium. Methods. Laboratory analysis, method of hema­gglutination reaction, analytical approach, statistical evaluation. Results. A change in lectin activity was determined in samples of 14 soft winter wheat varieties in caryopsis and at the early stages of ontogenesis (on the third, seventh, tenth and twelfth day of seedlings emergence). High lectin activity was observed in samples of seven and ten days seedlings. This could be evidence of active participation of proteins in physio­logical and biochemical processes at the time of emerging wheat tillering node that can be important in forming adaptive reactions to stress. Wheat lectin activity variation for рН ranging between 4,0 and 8,0 that depended on a variety was defined for the first time. Conclusions. According to hema­gglutination ability of wheat lectins, all varieties were divided into three groups: of high ability – 4 varieties, middle – 9 varieties, low – 1 variety. Varietal variability for the investigated trait can be used in breeding programs as marker trait. In most varieties variation of hemagglutination was observed at dilutions ranging between 1:2 and 1:256. Availability of two lectin agglutination peaks in acidic and alkaline media for seedlings was fixed.

Purpose. To study the dynamics of soft winter wheat lectin activity in caryopsis and at the early stages of ontogenesis of varieties developed by Poltava State Agrarian Academy as well as investigate varietal differences depending on dynamic pH medium. Methods. Laboratory analysis, method of hema­gglutination reaction, analytical approach, statistical evaluation. Results. A change in lectin activity was determined in samples of 14 soft winter wheat varieties in caryopsis and at the early stages of ontogenesis (on the third, seventh, tenth and twelfth day of seedlings emergence). High lectin activity was observed in samples of seven and ten days seedlings. This could be evidence of active participation of proteins in physio­logical and biochemical processes at the time of emerging wheat tillering node that can be important in forming adaptive reactions to stress. Wheat lectin activity variation for рН ranging between 4,0 and 8,0 that depended on a variety was defined for the first time. Conclusions. According to hema­gglutination ability of wheat lectins, all varieties were divided into three groups: of high ability – 4 varieties, middle – 9 varieties, low – 1 variety. Varietal variability for the investigated trait can be used in breeding programs as marker trait. In most varieties variation of hemagglutination was observed at dilutions ranging between 1:2 and 1:256. Availability of two lectin agglutination peaks in acidic and alkaline media for seedlings was fixed.