A method by GC/MS has been established for the determination of dimethenamid and cyhalofop-butyl in agricaltural products. Dimethenamid and cyhalofop-butyl were extracted with acetone, concentrated, and re - extracted with n - hexane. The extracts were cleaned up acetonitrile partitioning and florisil column, then determined by GC/MS-SIM. This method was featured of recovery over 75% and it's C.V. below 6%. The detection limits for dimetenamid and cyharofop-butyl were 0.001 micro g/g and 0.002 micro g/g, respectively.

In Nagano Prefecture, the sudden death of many wild birds became appearent in 1997. In order to investigate the cause, the authors developed a simultaneous analytical method using GC/MS. The method was applied to investigate the reason of sudden wild bird death in 3 cases that happened in 1998. Therefore, the every cause was cleared as following. 1) In the case of Komagane City, seeds of buckwheat were found in the residues of their stomachs, and the buckwheat was subjected to analyses. A high concentration of highly toxic insecticide, Methomyl, was detected. The authors presumed that was the cause of birds' death. 2) In the case of Saku City, some flesh, probably from apples was found in the birds' stomachs. The result of flesh analysis was the highly toxic insecticide, EPN, detected in a high concentration. The authors assumed that was the cause of death. 3) In the case of Asashina Village, fish, shrimp, rice, among others were found in the birds' stomachs. The result of their analysis was the highly toxic insecticide, Benfuracarb, detected in a high concentration. The authors assumed that was the cause of death.

A micro analytical method has been established for aliphatic lower primary and secondary amines in environmental water and sediment. Amines were distilled and allowed to react with benzenesulfonyl chloride under an alkaline condition. The derivatized compounds were extracted with benzene under an acidic condition and subjected to a determination by GC/MS. This method has been successfully applied for a simultaneous determination of primary and secondary amines with recovery over 80%, except for methylamine, and C.V. below 7%, except for methyl and dimethyl amines; moreover, the detection limit were 0.1 micro g/L for all of the amines determinated. This method is excelent also in sensitivity, is sufficiently applicable to environmental samples.

Analytical method for hydrophilic polar environmental pollutants was studied. Among these pollutants, the halogenated lower carboxylates were the target compounds of this investigation. A novel but simple derivatization method necessary for sample preparation for instrumental analysis was developed. The new derivatization method for the carboxylates is not a conventional chemical reaction in non-aqueous solvent following isolation from the environmental samples but rather, a direct aqueous reaction ; direct conversion of the carboxylates to amides using a carbodiimide in dilute aqueous solution. Halogenoanilines were selectively used with dicyclohexylcarbodiimide (DCC) under acidic conditions at room temperature to form the anilide derivatives. Moreover, this reaction proceeds while mixing the sample water, DCC, a halogenoaniline, and small volume of extracting solvent. Thus, for the first time, simultaneous derivatization of the carboxylates and extraction of the products into solvent was achieved. Based on the results, simple and practical determination methods by gas chromatography were developed for traces of these carboxylates in environmental samples and the proposed methods were applied to some real samples. Such analytical derivatization methods for carboxylates are useful and noteworthy because they can form C-N bonds in aqueous media under mild conditions with carbodiimides and N atoms of amino and other compounds.