Nitric oxide (NO) and ethylene are both important signaling molecules which participate in numerous plant development processes and environmental stress resistance. Here, we investigate whether and how NO interacts with ethylene during the development of endodermal barriers that have major consequences for the apoplastic uptake of cadmium (Cd) in the hyperaccumulator Sedum alfredii. In response to Cd, an increased NO accumulation, while a decrease in ethylene production was observed in the roots of S. alfredii. Exogenous supplementation of NO donor SNP (sodium nitroprusside) decreased the ethylene production in roots, while NO scavenger cPTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) had the opposite effect. The exogenous addition of NO affected the ethylene production through regulating the expression of genes related to ethylene synthesis. However, upon exogenous ethylene addition, roots retained their NO accumulation. The abovementioned results suggest that ethylene is downstream of the NO signaling pathway in S. alfredii. Regardless of Cd, addition of SNP promoted the deposition of endodermal barriers via regulating the genes related to Casparian strips deposition and suberization. Correlation analyses indicate that NO positively modifies the formation of endodermal barriers via the NO-ethylene signaling pathway, Cd-induced NO accumulation interferes with the synthesis of ethylene, leading to a deposition of endodermal barriers in S. alfredii.

The blood–follicle barrier (BFB) between the blood and follicular fluid (FF) can maintain the microenvironment balance of oocyte. Boron, an exogenous environmental trace element, has been found to possibly play an important role in oocyte maturation. This study aimed to examine the distribution characteristics of boron across the BFB and find the potential effect of boron on FF microenvironment. We analyzed the concentration of boron in paired FF and serum collected from 168 women undergoing in vitro fertilization and embryo transfer in Beijing City and Shandong Province, China. To explore the potential health impact of boron enrichment in oocyte maturation, a global proteomics analysis was conducted to tentatively correlate the protein levels with the boron enrichment. Interestingly, the results showed that the concentration of boron in FF (34.5 ng/mL) was significantly higher than that in serum (22.0 ng/mL), with a median concentration ratio of 1.52. Likewise, the concentrations of boron in FF and serum were positively correlated (r = 0.446), suggesting that boron concentration in serum can represent its concentration in follicular fluid to a large extent.. This is the first time to observe the enrichment of boron in the FF to our knowledge. It is interesting to observe a total of 13 proteins, which mainly belong to immunoglobulin class, were positively correlated with boron concentration in FF. We concluded that boron, as one environmental trace element, was enriched in FF from blood validated by two area in north china, which may be involved in an increased level of immune processes of immunoglobulins.

The adsorption of radioactive iodine, which is capable of presenting high mobility in aquatic ecosystems and generating undesirable health effects in humans (e.g., thyroid gland dysfunction), was comprehensively examined using pristine spent coffee ground biochar (SCGB) and bismuth-impregnated spent coffee ground biochar (Bi@SCGB) to provide valuable insights into the variations in the adsorption capacity and mechanisms after pretreatment with Bi(NO₃)₃. The greater adsorption of radioactive iodine toward Bi@SCGB (adsorption capacity (Qₑ) = 253.71 μg/g) compared to that for SCGB (Qₑ = 23.32 μg/g) and its reduced adsorption capability at higher pH values provide evidence that the adsorption of radioactive iodine with SCGB and Bi@SCGB is strongly influenced by the presence of bismuth materials and the electrostatic repulsion between their negatively charged surfaces and negatively charged radioactive iodine (IO₃⁻). The calculated R² values for the adsorption kinetics and isotherms support that chemisorption plays a crucial role in the adsorption of radioactive iodine by SCGB and Bi@SCGB in aqueous phases. The adsorption of radioactive iodine onto SCGB was linearly correlated with the contact time (h¹/²), and the diffusion of intra-particle predominantly determined the adsorption rate of radioactive iodine onto Bi@SCGB (Cₛₜₐgₑ II (129.20) > Cₛₜₐgₑ I (42.33)). Thermodynamic studies revealed that the adsorption of radioactive iodine toward SCGB (ΔG° = −8.47 to −7.83 kJ/mol; ΔH° = −13.93 kJ/mol) occurred exothermically and that for Bi@SCGB (ΔG° = −15.90 to −13.89 kJ/mol; ΔH° = 5.88 kJ/mol) proceeded endothermically and spontaneously. The X-ray photoelectron spectroscopy (XPS) analysis of SCGB and Bi@SCGB before and after the adsorption of radioactive iodine suggest the conclusion that the change in the primary adsorption mechanism from electrostatic attraction to surface precipitation upon the impregnation of bismuth materials on the surfaces of spent coffee ground biochars is beneficial for the adsorption of radioactive iodine in aqueous phases.

Neonicotinoid insecticides (NNIs) had been detected in soil and surface water frequently because of extensive use worldwide, however, data regarding regional characteristics and potential influential factors of sediment were scarce. In the present study, eight NNIs were analyzed in 86 surface sediment samples from different regions (central cities, rural areas and suburbs) and land use types (construction land and crop land) in Jiangsu Province. NNIs were widespread in the sediments, with a mean value of 1.73 ± 0.89 ng g⁻¹ dry weight (dw) (ranged from 0.41 to 3.87 ng g⁻¹ dw). Imidaclothiz (IMIZ), dinotefuran (DIN) and nitenpyram (NIT) were the dominant compounds in the surface sediment, accounted for half of combined total. The results of regional distribution analysis show that NNIs were at higher concentrations in rural areas and crop land, while the residues of NNIs in lakes were more severe compare with rivers in Jiangsu Province. Region characteristics and land use types have an influence on residues of NNIs in surface sediment. Principal component analysis showed that residues of NNIs in surface sediment in Jiangsu Province mainly originated from protect grain crops (maize), fruit (apples, pears) and vegetables in agricultural systems. The residues of NNIs were found to be mostly concentrated in the northwest and northeast in Jiangsu Province, where were the area of intensive agriculture. To investigate the residues of NNIs, while identify the contributing factors, could provide a scientific basis for basic of region environment management and pollution control.

Coastal acidification is often much more intense than ocean acidification due to eutrophication. To better understand the relationship between long-term coastal acidification (CA) and coastal eutrophication (CE), in-situ monthly data over the past three decades (1986–2017) were analyzed from Hong Kong Coast (HKC). The coastwide annual mean pH change (ΔpHₘₑₐₙ) was estimated at −0.0085 ± 0.0069 unit·yr⁻¹ in last decades, which was over four times stronger than current estimation on open ocean acidification rate (∼−0.0019 unit·yr⁻¹). According to the CA spatial pattern, greater pH decline (ΔpHₘₑₐₙ = −0.017 ± 0.009 unit·yr⁻¹) occurred in northwest, central south and central east HKC areas, much higher than the less acidified (ΔpHₘₑₐₙ = −0.004 ± 0.002 unit·yr⁻¹) southwest and northeast HKC areas. The spatiotemporal CA variations were associated with water discharges, atmospheric CO₂ increase and respiration/production that was indicated by DIN:DIP structure changes. The annual mean DIN:DIP ratio increased progressively from initial ∼16 in 1986 to ∼37 in 2017, revealing excess nitrogen load from rapid urbanization in this region. Such discharge-induced acidification was estimated as the major contributor for the total CA in HKC over the last three decades. In addition, our simulation results indicated that a potential CA rate at ∼0.0035 unit·yr⁻¹ could be reached if reducing mean DIN:DIP from discharged water to ∼23 from HKC. This study revealed a previously not recognized relationship between coastal acidification and changing coastal nutrient stoichiometry, and proposed possible management approaches.

Stable isotope fractionation of toluene under dynamic phase exchange was studied aiming at ascertaining the effects of gas-liquid partitioning and biodegradation of toluene stable isotope composition in liquid-air phase exchange reactors (Laper). The liquid phase consisted of a mixture of aqueous minimal media, a known amount of a mixture of deuterated (toluene-d) and non-deuterated toluene (toluene-h), and bacteria of toluene degrading strain Pseudomonas putida KT2442. During biodegradation experiments, the liquid and air-phase concentrations of both toluene isotopologues were monitored to determine the observable stable isotope fractionation in each phase. The results show a strong fractionation in both phases with apparent enrichment factors beyond −800‰. An offset was observed between enrichment factors in the liquid and the gas phase with gas-phase values showing a stronger fractionation in the gas than in the liquid phase. Numerical simulation and parameter fitting routine was used to challenge hypotheses to explain the unexpected experimental data. The numerical results showed that either a very strong, yet unlikely, fractionation of the phase exchange process or a – so far unreported – direct consumption of gas phase compounds by aqueous phase microorganisms could explain the observed fractionation effects. The observed effect can be of relevance for the analysis of volatile contaminant biodegradation using stable isotope analysis in unsaturated subsurface compartments or other environmental compartment containing a gas and a liquid phase.

The properties and metal-binding abilities of dissolved organic matter (DOM) rely on its molecular weight (MW) structure. In this study, the spatial differences of DOM in compositions, MW structures, and binding mechanisms with copper (Cu²⁺) in Dongping Lake were investigated by applying excitation-emission matrix combining parallel factor analysis (EEM-PARAFAC), synchronous fluorescence (SF) spectra, two-dimensional correlation spectra (2D-COS), and Fourier transform infrared (FTIR) spectra. The EDOM for the entrance of the Dawen River and PDOM for the macrophyte-dominated region were divided from DOM of Dongping Lake based on hierarchical clustering analysis (HCA) and principal component analysis (PCA) and were size-fractioned into MW < 500 kDa and <100 kDa fractions. According to EEM-PARAFAC, Dongping Lake was dominated by tryptophan-like substances with MW < 500 kDa. The concentration of PDOM was higher than that of EDOM (p < 0.05). 2D-COS showed that protein-like components preceded humic-like components binding to Cu²⁺ regardless of sample type (215 nm > 285 nm > 310–360 nm). The Cu²⁺ binding capacity of DOM exhibited specific differences in space, components, and molecular weights. The humic-like component 1 (C1) and tryptophan-like component 4 (C4) of PDOM showed stronger binding abilities than those of EDOM. Endogenous tryptophan-like component 4 (C4) had a higher binding affinity for Cu²⁺ than humic-like components (logKₐ: C4 > C1 > C2) in PDOM irrespective of MW. Humic-like components with MW < 500 kDa displayed higher binding potentials for Cu²⁺. FTIR spectra showed that the main participants of DOM-Cu complexation included aromatic hydrocarbons, aliphatic groups, amide Ⅰ bands, and carboxyl functional groups. This study provides spatial-scale insights into the molecular weight structure of DOM in influencing the behavior, fate, and bioavailability of heavy metals in lakes.

Heavy metal toxicity has become an impediment to agricultural productivity, which presents major human health concerns in terms of food safety. Among them, arsenic (As) a non-essential heavy metal has gained worldwide attention because of its noxious effects on agriculture and public health. The increasing rate of global warming and anthropogenic activities have promptly exacerbated As levels in the agricultural soil, thereby causing adverse effects to crop genetic and phenotypic traits and rendering them vulnerable to other stresses. Conventional breeding and transgenic approaches have been widely adapted for producing heavy metal resilient crops; however, they are time-consuming and labor-intensive. Hence, finding new mitigation strategies for As toxicity would be a game-changer for sustainable agriculture. One such promising approach is harnessing plant microbiome in the era of ‘omics’ which is gaining prominence in recent years. The use of plant microbiome and their cocktails to combat As metal toxicity has gained widespread attention, because of their ability to metabolize toxic elements and offer an array of perquisites to host plants such as increased nutrient availability, stress resilience, soil fertility, and yield. A comprehensive understanding of below-ground plant-microbiome interactions and their underlying molecular mechanisms in exhibiting resilience towards As toxicity will help in identifying elite microbial communities for As mitigation. In this review, we have discussed the effect of As, their accumulation, transportation, signaling, and detoxification in plants. We have also discussed the role of the plant microbiome in mitigating As toxicity which has become an intriguing research frontier in phytoremediation. This review also provides insights on the advancements in constructing the beneficial synthetic microbial communities (SynComs) using microbiome engineering that will facilitate the development of the most advanced As remedial tool kit in sustainable agriculture.

Nickel (Ni) and manganese (Mn) are well known for the production of steel and alloys and are commonly found co-occurring in Ni ores. They are metals of environmental concern and contamination in the marine environment is problematic single exposures and in combination. Several studies have documented the effects of single metal exposure on the model anemone E. pallida, but research on the effects of metal mixtures is far less common. This novel study assesses the accumulation and stress effects of Ni and Mn over a 12-d exposure period. E. pallida were exposed in two separate experiments; Ni alone and Ni in combination with Mn, to assess accumulation, along with any effect on the density of symbionts and anemone tentacle length. Anemones were transferred to ambient seawater to assess depuration and recovery over 6 d. Anemone tissue accumulated Ni at a magnitude of five times higher in a mixture of 0.5 mg Ni/L with 2.5 mg Mn/L compared to the same concentration in a single Ni exposure experiment. In both experiments, Ni and Mn preferentially accumulated in the Symbiodinium spp. compared to the anemone tissue, but Ni depuration was more rapid in the mixture than Ni alone exposure. This study reveals a significant reduction in anemone Symbiodinium spp. density after exposure to Ni and Mn mixtures, but not with Ni exposure alone. A significant dose-dependent reduction in tentacle length was observed in anemones after 12 d of the Ni exposure both with and without Mn. The estimated sublethal concentration that causes tentacle retraction in 50% of test anemones (EC50) by Ni was 0.51 (0.25–0.73) mg/L, while in combination with Mn the EC50 was 0.30 mg Ni/L (confidence limits not calculatable). The present data reveals the importance of testing metal effects in combination before establishing safe limits for marine invertebrates.

Sewage treatment plants (STPs) are considered as “hotspots” for the emergence and proliferation of antibiotic resistance. However, the impact of heavy metals contamination on dispersal of antibiotic resistance in STPs is poorly understood. This study simultaneously investigated the effect of removal of metal and antibiotic resistance as well as mobile elements at different treatment units of STPs in Delhi, India. Results showed that treatment technologies used in STPs were inefficient for the complete removal of metal and antibiotic resistance, posing an ecological risk of co-selection of antibiotic resistance. The strong correlations were observed between heavy metals, metal and antibiotic resistance, and integrons, implying that antibiotic resistance may be exacerbated in the presence of heavy metals via integrons, and that metal and antibiotic resistance share a common or closely associated mechanism. We quantified an MRG rcnA, conferring resistance to Co and Ni, and identified that it was more abundant than all MRGs, ARGs, integrons, and 16S rRNA, suggesting rcnA could be important in antibiotic resistance dissemination in the environment. The associations between heavy metals, metal and antibiotic resistance, and integrons highlight the need for additional research to better understand the mechanism of co-selection as well as to improve the removal efficacy of current treatment systems.