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Acute toxicity of povidone-iodine (Betadine) in common carp (Cyprinus carpio L. 1758)
2017
Forouhar Vajargah, Mohammad | Mohammadi Yalsuyi, Ahmad | Hedayati, Aliakbar
Organisms in aquatic environments are exposed to a number of pollutants such as pharmaceutical residues. As such, the aim of the present study is to achieve the Lethal Concentration (LC50) of Povidone-iodine (Betadine) for Cyprinus carpio. To do so, the study employs samples, weighing 4±1 [mean±SD] gr, and carries out an experiment in static condition. Based on OECD instructions, after a period of 4 days under controlled water, the physicochemical factors give the following results: pH= 8-8.3, BOD= 690 mg/l, total hardness= 210 mg, and CaCo3 and temperature= 17±0.1 °C. All fish are acclimatized for 10 days in an aquarium, 60×55×30 cm in size, which included the control group (no toxic concentration) as well as the treated aquariums, with Betadine concentration of 20, 40, 60, 80, 100, 120, 140, 160, 180, 200, 400, and 600 mg/l. LC10, LC20, LC30, LC40, LC50, LC60, LC70, LC80, LC90, and LC95 have been measured for 6, 12, 18, and 24 hours. LC50 24h Betadine for C. carpio has been 158.273 ml/l, showing no mortality after 24 hours (i.e. 48h, 72h, and 96h). Results of the present study suggest that Betadine is practically nontoxic and not irritant at low concentrations for this species and it has a short half-life in aquatic environments.
Show more [+] Less [-]Simultaneous removal of heterocyclic drugs and total nitrogen from biochemical tailwater by peracetic acid/cobalt-loaded ceramsite-based denitrification biofilter
2022
Li, Tong | Jin, Lili | Zhu, Shanshan | Zhang, Xuxiang | Ren, Hongqiang | Huang, Hui
It is difficult to achieve simultaneous and efficient removal of heterocyclic drugs (HCDs) and total nitrogen (TN) in conventional denitrification biofilter (DNBF). Inspired by the effective degradation of refractory organic matter by cobalt-based advanced oxidation process and the need for in-situ upgrading of DNBF, peracetic acid (PAA)/cobalt-loaded ceramsite-based DNBF system was constructed for the first time to treat biochemical tailwater containing HCDs. Results showed that PAA/Co-DNBF had relatively high removal rates for the four HCDs with the order of CBZ > TMP > SDZ > SMX, and the optimal DNBF was H2 with 150 μg L⁻¹of PAA. Overall, TN and HCDs removal increased by 178%–455% and 2.50%–40.99% respectively. When the influent concentration of NO₃⁻-N, COD and each HCDs of 20 mg/L, 60 mg/L and 20 μg/L, below 15 mg/L of effluent TN and the highest average removal rate of SMX (67.77%) could be achieved, under HRT of 4 h in H2. More even distribution of microbial species and low acute toxicity of effluent were also achieved. More even distribution of microbial species and low acute toxicity of effluent were also achieved. In addition, high extracellular polymeric substance (EPS) content and Gordonia after the addition of PAA contributed to the degradation of HCDs. This study supplied a potentially effective strategy for the treatment of biochemical tailwater containing HCDs and provided new insight into the advance of denitrification technology.
Show more [+] Less [-]Novel treatment of Microcystis aeruginosa using chitosan-modified nanobubbles
2022
Nam, Gwiwoong | Mohamed, Mohamed M. | Jung, Jinho
In this study, we treated harmful Microcystis aeruginosa cyanobacteria using chitosan-modified nanobubbles. The chitosan-modified nanobubbles (255 ± 19 nm) presented a positive zeta potential (15.36 ± 1.17 mV) and generated significantly (p < 0.05) more hydroxyl radicals than the negatively charged nanobubbles (−20.68 ± 1.11 mV). Therefore, the interaction between the positively charged chitosan-modified nanobubbles and negatively charged M. aeruginosa (−34.81 ± 1.79 mV) was favored. The chitosan-modified nanobubble treatment (2.20 × 10⁸ particles mL⁻¹) inactivated 73.16% ± 2.23% of M. aeruginosa (2.00 × 10⁶ cells mL⁻¹) for 24 h without causing significant cell lysis (≤0.25%) and completely inhibited the acute toxicity of M. aeruginosa toward Daphnia magna. The inactivation was correlated (r² = 0.97) with the formation of reactive oxygen species (ROS) in M. aeruginosa. These findings indicated that the hydroxyl radicals generated by the chitosan-modified nanobubbles disrupted cell membrane integrity and enhanced oxidative stress (ROS formation), thereby inactivating M. aeruginosa. Moreover, the penetration of the chitosan-modified nanobubbles and cell alterations in M. aeruginosa were visually confirmed. Our results suggested that the chitosan-modified nanobubble treatment is an eco-friendly method for controlling harmful algae. However, further studies are required for expanding its practical applications.
Show more [+] Less [-]Suspended solids-associated toxicity of hydraulic fracturing flowback and produced water on early life stages of zebrafish (Danio rerio)
2021
Lü, Yichun | Zhang, Yifeng | Zhong, Cheng | Martin, Jonathan W. | Alessi, Daniel S. | Goss, Greg G. | Ren, Yuan | He, Yuhe
Hydraulic fracturing flowback and produced water (HF-FPW), which contains polyaromatic hydrocarbons (PAHs) and numerous other potential contaminants, is a complex wastewater produced during the recovery of tight hydrocarbon resources. Previous studies on HF-FPW have demonstrated various toxicological responses of aquatic organisms as consequences of combined exposure to high salinity, dissolved organic compounds and particle/suspended solids-bound pollutants. Noteworthy is the lack of studies illustrating the potentially toxic effects of the FPW suspended solids (FPW-SS). In this study, we investigated the acute and sublethal toxicity of suspended solids filtered from six authentic FPW sample collected from two fracturing wells, using a sediment contact assay based on early-life stages of zebrafish (Danio rerio). PAHs profiles and acute toxicity tests provided initial information on the toxic potency of the six samples. Upon exposure to sediment mixture at two selected doses (1.6 and 3.1 mg/mL), results showed adverse effects in larval zebrafish, as revealed by increased Ethoxyresorufin-O-deethylase (EROD) activity. Transcriptional alterations were also observed in xenobiotic biotransformation (ahr, pxr, cyp1a, cyp1b1, cyp1c1, cyp1c2, cyp3a65, udpgt1a1, udpgt5g1), antioxidant response (sod1, sod2, gpx1a, gpx1b) and hormone receptor signaling (esr1, esr2a, cyp19a1a, vtg1) genes. The results demonstrated that even separated from the complex aqueous FPW mixture, FPW-SS can induce toxicological responses in aquatic organisms' early life stages. Since FPW-SS could sediment to the bottom of natural wetland acting as a continuous source of contaminants, the current findings imply the likelihood of long-term environmental risks of polluted sediments on aquatic ecosystems due to FPW spills.
Show more [+] Less [-]Influence of fuel oil on Platymonas helgolandica: An acute toxicity evaluation to amino acids
2021
Li, Na | Liu, Yu | Liang, Zhengyu | Lou, Yadi | Liu, Yuxin | Zhao, Xinda | Wang, Guoguang
It is highly likely that the toxicity of water accommodated fractions (WAF) will influence marine microalgae, and consequently lead to potential risk for the marine ecological environment. However, it was often neglected whether WAF can influence the transformation of relative compounds in organisms. The metabolism of amino acids (AAs) can be used to track physiological changes in microalgae because amino acids are the basis of proteins and enzymes. In this study, using marine Chlorophyta Platymonas helgolandica as the test organism, the effects of different concentrations of WAF on AA compositions and stable carbon isotope ratios (δ¹³C) of individual AAs of Platymonas helgolandica were investigated. The results showed that the WAF of #180 fuel oil had an obvious suppressing effect on the growth and chlorophyll a content of microalgae. The growth inhibitory rate at 96 h was 80.66% at a WAF concentration of 0.50 mg L⁻¹ compared with the control. Furthermore, seven among the 16 AAs, including alanine, cysteine, proline, aspartic acid, lysine, histidine and tyrosine, had relatively high abundance. Under the glycolysis pathway, the cysteine abundance was higher than control, meaning that the biosynthesized pathway of alanine through cysteine as a precursor could be damaged. Phosphoenolpyruvate (PEP) was an important synthesis precursor of alanine (leucine) and aromatic AA family (Phenylalanine and tyrosine), and played an important role in δ¹³CAAₛ fractionation under the WAF stress. Under the TCA pathway, to protect cell metabolism activities under WAF stress, the δ¹³C value of threonine and proline abundance in microalgae with the increase in WAF stress. Therefore, δ¹³CAAₛ fractionation can be used as a novel method for toxicity evaluation of WAF on future.
Show more [+] Less [-]Enantioselective residues and toxicity effects of the chiral triazole fungicide hexaconazole in earthworms (Eisenia fetida)
2021
Liu, Tong | Fang, Kuan | Liu, Yalei | Zhang, Xiaolian | Han, Lingxi | Wang, Xiuguo
The enantioselective toxic effect and environmental behavior of chiral pesticides have attracted increasing research attention. In this study, the enantioselective toxicity and residues of hexaconazole (HEX) in earthworms (Eisenia fetida) were investigated. In the present study, significant enantioselective degradation characteristics were observed in artificial soil with the R-enantiomer preferentially degrading (p < 0.05); however, no significant enantioselective bioaccumulation was observed in the earthworms (p > 0.05). The acute toxicity of S-(+)-HEX was higher than that of R-(−)-HEX in earthworms, with 48-h LC₅₀ values of 8.62 and 22.35 μg/cm², respectively. At 25 mg/kg, enantiospecific induction of oxidative stress was observed in earthworms; moreover, S-(+)-HEX had a greater influence on the contents of malonaldehyde, cytochrome P450, and 8-hydroxy-2-deoxyguanosine than R-(−)-HEX. These results were consistent with those of the enrichment analysis of differentially expressed genes. The transcriptome sequencing results showed that S-(+)-HEX had a more significant influence on steroid biosynthesis, arachidonic acid metabolism, and cell cycle processes than R-(−)-HEX, leading to abnormal biological function activities. These results indicate that S-(+)-HEX may pose a higher risk to soil organisms than R-(−)-HEX. This study suggests that the environmental risk of chiral pesticides to nontarget organisms should be assessed at the enantiomeric level.
Show more [+] Less [-]Valorization of biodiesel side stream waste glycerol for rhamnolipids production by Pseudomonas aeruginosa RS6
2021
Baskaran, Shobanah Menon | Zakaria, Mohd Rafein | Mukhlis Ahmad Sabri, Ahmad Syafiq | Mohamed, Mohd Shamzi | Wasoh, Helmi | Toshinari, Maeda | Hassan Mohd. Ali, | Banat, Ibrahim M.
Biodiesel side stream waste glycerol was identified as a cheap carbon source for rhamnolipids (RLs) production which at the same time could improve the management of waste. The present study aimed to produce RLs by using Pseudomonas aeruginosa RS6 utilizing waste glycerol as a substrate and to evaluate their physico-chemicals properties. Fermentation conditions such as temperature, initial medium pH, waste glycerol concentration, nitrogen sources and concentrations resulted in different compositions of the mono- and di-RLs produced. The maximum RLs production of 2.73 g/L was obtained when P. aeruginosa RS6 was grown in a basal salt medium supplemented with 1% waste glycerol and 0.2 M sodium nitrate at 35 °C and pH 6.5. At optimal fermentation conditions, the emulsification index (E₂₄) values of cooking oil, diesel oil, benzene, olive oil, petroleum, and kerosene were all above E₂₄₌50%. The surface tension reduction obtained from 72.13 mN/m to 29.4–30.4 mN/m was better than the surface activity of some chemical-based surfactants. The RLs produced possessed antimicrobial activities against Gram-negative and Gram-positive bacteria with values ranging from 37% to 77% of growth inhibition when 1 mg/mL of RLs was used. Concentrations of RLs below 1500 μg/mL did not induce phytotoxicity effects on the tested seeds (Vigna radiata) compared to the chemical-based- surfactant, SDS. Furthermore, RLs tested on zebrafish (Danio rerio) embryos only exhibited low acute toxicity with an LC₅₀ value of 72.97 μg/mL at 48 h of exposure suggesting a green and eco-biochemical worthy of future applications to replace chemical-based surfactants.
Show more [+] Less [-]Understanding the effects of hydraulic fracturing flowback and produced water (FPW) to the aquatic invertebrate, Lumbriculus variegatus under various exposure regimes
2020
Mehler, W Tyler | Nagel, Andrew | Flynn, Shannon | Zhang, Yifeng | Sun, Chenxing | Martin, Jonathan | Alessi, Daniel | Goss, Greg G.
Hydraulic fracturing of horizontal wells is a cost effective means for extracting oil and gas from low permeability formations. Hydraulic fracturing often produces considerable volumes of flowback and produced water (FPW). FPW associated with hydraulic fracturing has been shown to be a complex, often brackish mixture containing a variety of anthropogenic and geogenic compounds. In the present study, the risk of FPW releases to aquatic systems was studied using the model benthic invertebrate, Lumbriculus variegatus and field-collected FPW from a fractured well in Alberta. Acute, chronic, and pulse toxicity were evaluated to better understand the implications of accidental FPW releases to aquatic environments. Although L.variegatus is thought to have a high tolerance to many stressors, acute toxicity was significant at low concentrations (i.e. high dilutions) of FPW (48 h LC50: 4–5%). Chronic toxicity (28 d)of FPW in this species was even more pronounced with LC50s (survival/reproduction) and EC50s (total mass) at dilutions as low as 0.22% FPW. Investigations evaluating pulse toxicity (6 h and 48 h exposure) showed a significant amount of latent mortality occurring when compared to the acute results. Additionally, causality in acute and chronic bioassays differed as acute toxicity appeared to be primarily driven by salinity, which was not the case for chronic toxicity, as other stressors appear to be important as well. The findings of this study show the importance of evaluating multiple exposure regimes, the complexity of FPW, and also shows the potential aquatic risk posed by FPW releases.
Show more [+] Less [-]Predicting zebrafish spatial avoidance triggered by discharges of dairy wastewater: An experimental approach based on self-purification in a model river
2020
Silva, Daniel C.V.R. | Queiroz, Lucas G. | Marassi, Rodrigo J. | Araújo, Cristiano V.M. | Bazzan, Thiago | Cardoso-Silva, Sheila | Silva, Gilmar C. | Müller, M. | Silva, Flávio T. | Montagner, Cassiana C. | Paiva, Teresa C.B. | Pompêo, Marcelo L.M.
Wastewater discharges from dairy industries can cause a range of harmful effects in aquatic ecosystems, including a decline in biodiversity due to species evasion. Therefore, it is important to know the purification potential of rivers for the removal of pollutants released in dairy wastewater (DWW). The hypothesis adopted in this work was that the release of DWW into stretches of the Ribeirão dos Pombos River (São Paulo State, Brazil) might trigger an avoidance response, resulting in fish migrating to other regions, with the response being greater when the self-cleaning potential of the river is smaller. Therefore, the goals of the present study were to: (i) investigate how land use and seasonality of the rainfall regime influence the quality of the water in different areas of the river (P1: river source; P2: urban region; P3: rural region); (ii) assess the potential of the river to purify DWW; and (iii) evaluate the potential toxicity and repellency of DWW to the freshwater fish Danio rerio, using acute toxicity (mortality) and non-forced avoidance tests, respectively. P1 was shown to be the most preserved area. The chemical composition of the river varied seasonally, with higher concentrations of Cl⁻ and SO₄²⁻ at P3 during the rainy period. The river purification potential for DWW was higher at P2, due to greater microbiological activity (associated with higher BOD). The DWW was more acutely toxic in water from P2. The avoidance response was strongly determined by the concentration of DWW, especially for water from P2. The high capacity for self-cleaning at P2 did not seem sufficient to maintain the stability of the ecosystem. Finally, the non-forced exposure system proved to be a suitable approach that can assist in predicting how contaminants may affect the spatial distributions of organisms.
Show more [+] Less [-]Enzyme assays and toxicity of pig abattoir waste in Eisenia andrei
2020
Ramires, Maiara Figueiredo | Lorensi de Souza, Eduardo | de Castro Vasconcelos, Márlon | Clasen, Bárbara Estevão | Fontanive, Daniel Erison | Bianchetto, Renan | Grasel Cezimbra, Júlio Cesar | Antoniolli, Zaida Inês
Due to high global demand, large amounts of abbattoir waste are generated from pork production. Mismanagement of abattoir waste on agricultural lands can result in soil and water contamination with pathogens and contaminants like metals and nutrients. Therefore, possible effects on soil organisms prior to application should be evaluated. Thus, the aim of this study was to determine the effects of fresh pig abattoir waste (PAWf) and waste after stabilization processes on E. andrei through tests of avoidance behavior, acute toxicity and chronic toxicity. In order to do this, the waste was evaluated fresh (i.e., non-treated), and after aerated composting (PAWa), natural composting (PAWn) and vermicomposting (PAWv). In addition, we used a natural soil with no history of agricultural use as control soil. The evaluation was based on avoidance behavior, mortality, initial and final earthworm weight, and reproduction, in addition to a set of enzyme assays formed by acetylcholinesterase, lipid peroxidation, catalase and glutathione S-transferase measured over time. The ecotoxicological results showed that PAWf and PAWa increased AChE activity at different experimental periods, while PAWn decreased activity at 14 days compared to the control. PAWf and PAWa increased TBARS levels at 7 and 14 days, respectively. CAT activity decreased at 3, 7 and 14 days in PAWv, while GST activity increased at 3 days in PAWa and at 3 and 14 days in PAWf compared to the control. In the acute toxicity test, PAWa and PAWn had a toxic effect on E. andrei, resulting in 100% mortality at 14 days of exposure. Based on our findings, pig abattoir waste should undergo vermicomposting prior to agricultural application to soils.
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