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Acute exposure to Buenos Aires air particles (UAP-BA) induces local and systemic inflammatory response in middle-aged mice: A time course study
2016
Orona, Nadia S. | Ferraro, Sebastián A. | Astort, Francisco | Morales, Celina | Brites, Fernando | Boero, Laura | Tiscornia, Gisela | Maglione, Guillermo A. | Saldiva, Paulo H.N. | Yakisich, Sebastian | Tasat, Deborah R.
Exposure to air particulate matter (PM) is associated with increased cardiovascular morbimortality. However, PM doesn't affect equally to all people, being the old cohort the most susceptible and studied. We hypothesized that another specific life phase, the middle-aged subpopulation, may be negatively affected. Therefore, the aim of this study was to analyze in vivo the acute biological impact of two environmental particles, Urban Air Particles from Buenos Aires and Residual Oil Fly Ash, on the cardiorespiratory system of middle-aged mice, evaluating oxidative metabolism and inflammation. Both PM provoked a local and systemic inflammatory response, leading to a reduced alveolar area in the lung, an epicard inflammation in the heart, an increment of IL-6, and a reduction on PON 1 activity in serum of middle-aged animals. The positive correlation of local parameters with systemic markers of oxidative stress and inflammation could be responsible for associations of cardiovascular morbimortality in this subpopulation.
Show more [+] Less [-]Uptake, metabolism and sub-lethal effects of BDE-47 in two estuarine invertebrates with different trophic positions
2016
Díaz-Jaramillo, M. | Miglioranza, K.S.B. | Gonzalez, M. | Barón, E. | Monserrat, J.M. | Eljarrat, E. | Barceló, D.
Two microcosm types -sediment-biota and biota-biota- were constructed to simulate different pathways of BDE-47 uptake, metabolism and oxidative stress effects in two key estuarine invertebrates (polychaete Laeonereis acuta and crab Cyrtograpsus angulatus). In the sediment-biota experiment, both species were exposed to spiked sediments; an environmentally reported and a high concentration of BDE-47 for 2 weeks. In the biota-biota experiment, crabs were fed with polychaetes pre-exposed to BDE-47 in the sediment-biota experiment. The sediment-biota experiment first revealed that polychaetes significantly accumulated BDE-47 (biota-sediment accumulation factor >2; p < 0.05) to a much greater extent than the crab organs (muscle, hepatopancreas, gills) at both sediment concentrations. For oxidative stress responses, polychaete and crab tissues exposed to spiked sediment showed a significant increase (p < 0.05) of only glutathione S-transferase (GST) activity with respect to controls in both BDE-47 concentrations. No lipid peroxidation (TBARS) or total antioxidant capacity (ACAP) changes were evident in the species or organs exposed to either BDE-47 sediment concentration. The biota-biota experiment showed that feeding crabs with pre-exposed polychaetes caused BDE-47 accumulation in organs as well as significant amounts of BDE-47 eliminated through feces (p < 0.05). Unlike the sediment-biota exposure, crabs fed with pre-exposed BDE-47 polychaetes showed the most conspicuous oxidative stress responses. Significant changes in GST and ACAP in both hepatopancreas and gills, in addition to enhanced TBARS levels in the hepatopancreas with respect to controls (p < 0.05), revealed that BDE-47 assimilated by invertebrates represents a potential source of toxicity to their predators. No methoxylated metabolites (MeO-PBDEs) were detected during BDE-47 metabolism in the invertebrates in either of the two different exposure types. In contrast, hydroxylated metabolites (OH-PBDEs) were detected in polychaetes and crab organs/feces in both experiments. Our results demonstrate that PBDE hydroxylation is one of the main biotransformation routes of BDE-47 in estuarine animals, which could be associated with the oxidative stress responses found.
Show more [+] Less [-]Impact of Precipitation Intensity and Riparian Geomorphic Characteristics on Greenhouse Gas Emissions at the Soil-Atmosphere Interface in a Water-Limited Riparian Zone
2016
Vidon, P. | Marchese, S. | Welsh, M. | McMillan, S.
As concentrations of greenhouse gases (GHG: N₂O, CO₂, CH₄) continue to increase in the earth’s atmosphere, there is a need to further quantify the contribution of natural systems to atmospheric GHG concentrations. Within this context, characterizing GHG contributions of riparian zones following storms events is especially important. This study documents soil GHG effluxes in a North Carolina riparian zone in the days following both a natural 2.5-cm precipitation event, and that same event associated with the addition of 8.7 cm artificial rainwater in select static chambers. No significant differences in CO₂, CH₄, and N₂O fluxes in response to increased moisture were observed between a depression, a sand bar, and an upland forested area. However, in this water-limited riparian zone, less negative CH₄ fluxes (i.e., methane oxidation decreased) and higher CO₂ fluxes (i.e., aerobic respiration increased) were observed following precipitation. A short-term burst in N₂O emission was observed in the hours after precipitation occurred, but elevated N₂O emissions did not persist long enough to turn the site from the N₂O sink to a N₂O source in the 3 days following the beginning of the experiment. Our results are in contrast with riparian GHG studies in wetter environments and illustrate the importance of water limitation in regulating riparian soil response to precipitation with respect to GHG emissions. More studies should be conducted in water-limited environments (e.g., US southeast/southwest) before management strategies commonly applied in wetter environments (e.g., US Northeast/Midwest) are applied in these regions.
Show more [+] Less [-]Pentachlorophenol Biodegradation by Citrobacter freundii Isolated from Forest Contaminated Soil
2016
WerheniAmmeri, Rim | MokniTlili, Sonia | Mehri, Ines | Badi, Souhir | Hassen, Abdennaceur
Environmental pollution by pentachlorophenol (PCP) is a critical concern worldwide, and microbial bioremediation could constitute an ecologically friendly solution. The main objectives of this study were at first to clarify the factors, affecting the ability and efficiency of PCP biodegradation by the bacterium isolate P6, and secondly to optimize the condition of using P6 for PCP bioremediation. The PCP mineralizing bacterium was isolated from the contaminated forest soil of Tunisia, and it was identified as Citrobacter freundii (C. freundii), by using conventional and molecular characteristics. The HPLC and spectroscopic analysis were used to investigate the PCP degradation and the biomass formation by this isolate P6. The main results showed that P6 was able to degrade or to transform more than 98 % of 640 mg/l PCP afterwards 168 h in mineral salt medium (MSM). As well, the optimal aerobic growth conditions of P6 in MSM include essentially the range of pH (4 ≤ pH ≤ 9) and of temperature (25 °C < temperature < 30 °C). The addition of glucose as extra carbon sources has an effect to enhance the PCP biodegradation. On the other side, this isolate of C. freundii is capable to remove or transform around 95.33 % of PCP added in the sterilized soil suspension supplemented with PCP and adjusted to a final concentration of around 400 mg/l during 2 weeks of incubation at 25 °C. This last result argues in favor of the use of this strain P6 of C. freundii as a microbial tool of remediation of PCP-contaminated site.
Show more [+] Less [-]Phenol Degradation by Suspended Biomass in Aerobic/Anaerobic Electrochemical Reactor
2016
Ailijiang, Nuerla | Chang, Jiali | Wu, Qing | Li, Peng | Liang, Peng | Zhang, Xiaoyuan | Huang, Xia
The effect of direct current (DC) on phenol biodegradation under aerobic/anaerobic condition was investigated in this study using a bioelectrochemical reactor. It was found that phenol biodegradation was inhibited with current ranged from 10 to 40 mA. The growth of biomass was reduced to 43.2 ± 6.6 % for aerobic sludge and 38.6 ± 7.3 % for anaerobic sludge, but the loosely bound extracellular polymer substances (LB–EPS) were increased 91.2 ± 1.3 % for aerobic sludge and 62.8 ± 0.8 % for anaerobic sludge as the current increased from 10 to 40 mA. Adenosine triphosphate (ATP) content of aerobic sludge was also reduced 0.481 ± 0.04-fold and 0.512 ± 0.05-fold lower and 1.34 ± 0.13-fold higher than that of the control when the current was increased from 10 to 40 mA. The results of phosphate buffer saline adding treatment indicated that lower pH caused by a DC above 10 mA was responsible for the reduced phenol biodegradation, leading to the reduction of biomass. However, lower intensity of current (5 mA) had no significant impact on phenol degradation rate, pH, LB–EPS, ATP content, and cell growth of aerobic/anaerobic sludge. These results give us a more detailed understanding of the effects of electricity on the treatment of phenol containing wastewater.
Show more [+] Less [-]Polychlorinated biphenyl 126 exposure in L6 myotubes alters glucose metabolism: a pilot study
2016
Mauger, Jean-François | Nadeau, Lucien | Caron, Audrey | Chapados, Natalie Ann | Aguer, Céline
Polychlorinated biphenyls (PCBs) are increasingly recognized as metabolic disruptors. Due to its mass, skeletal muscle is the major site of glucose disposal. While muscle mitochondrial dysfunction and oxidative stress have been shown to play a central role in metabolic disease development, no studies to date have investigated the effect of PCB exposure on muscle energy metabolism and oxidative stress. In this pilot study, we tested the effect of exposure to PCB126 in L6 myotubes (from 1 to 2500 nM for 24 h) on mitochondrial function, glucose metabolism, and oxidative stress. Exposure to PCB126 had no apparent effect on resting, maximal, and proton leak-dependent oxygen consumption rate in intact L6 myotubes. However, basal glucose uptake and glycolysis were inhibited by 20–30 % in L6 myotubes exposed to PCB126. Exposure to PCB126 did not appear to alter skeletal muscle anti-oxidant defense or oxidative stress. In conclusion, our study shows for the first time that exposure to a dioxin-like PCB adversely affects skeletal muscle glucose metabolism. Given the importance of skeletal muscle in the maintenance of glucose homeostasis, PCB126 could play an important role in the development of metabolic disorders.
Show more [+] Less [-]Leaf-based physiological, metabolic, and ultrastructural changes in cultivated cotton cultivars under cadmium stress mediated by glutathione
2016
Daud, M. K. | Mei, Lei | Azizullah, Azizullah | Dawood, Muhammad | Ali, Imran | Mahmood, Qaisar | Ullah, Waheed | Jamīl, Muḥammad | Zhu, S. J.
Cadmium (Cd) pollution is present in the world over especially in the industrialized parts of the world. To reduce Cd accumulation in various crops especially food crops, alleviating agents such as reduced glutathione (GSH) can be applied, which are capable either to exclude or to sequester Cd contamination. This study investigated the leaf-based spatial distribution of physiological, metabolic, and microstructural changes in two cotton cultivars (Coker 312 and TM-1) under GSH-mediated Cd stress using single levels of Cd (50 μM) and GSH (50 μM) both separately and in mix along with control. Results showed that GSH revived the morphology and physiology of both cotton cultivars alone or in mix with Cd. Cd uptake was enhanced in all segments of leaf and whole leaf upon the addition of GSH. GSH alleviated Cd-induced reduction in the photosynthetic pigment compositions and chlorophyll a fluorescence parameters. Mean data of biomarkers (2,3,5-triphenyltetrazolium (TTC), total soluble protein (TSP), malondialdehyde (MDA), hydrogen peroxide (H₂O₂)) revealed the adverse effects of Cd stress on leaf segments of both cultivars, which were revived by GSH. The oxidative metabolism induced by Cd stress was profoundly influenced by exogenous GSH application. The microstructural alterations were mainly confined to chloroplastic regions of leaves under Cd-stressed conditions, which were greatly revived upon the GSH addition. As a whole, Cd stress greatly affected TM-1 as compared to Coker 312. These results suggest a positive role of GSH in alleviating Cd-mediated changes in different leaf sections of cotton cultivars.
Show more [+] Less [-]PAMAM dendrimers as nano carriers to investigate inflammatory responses induced by pulmonary exposure of PCB metabolites in Sprague-Dawley rats
2016
Wangpradit, Orarat | Adamcakova-Dodd, Andrea | Heitz, Katharina | Robertson, Larry | Thorne, Peter S. | Luthe, Gregor
Polychlorinated biphenyls (PCBs) persist and accumulate in the ecosystem depending upon the degree of chlorination of the biphenyl rings. Airborne PCBs are especially susceptible to oxidative metabolism, yielding mono- and di-hydroxy metabolites. We have previously demonstrated that 4-chlorobiphenyl hydroquinones (4-CB-HQs) acted as cosubstrates for arachidonic acid metabolism by prostaglandin H synthase (PGHS) and resulted in an increase of prostaglandin production in vitro. In the present study, we tested the capability of 4-CB-HQ to act as a co-substrate for PGHS catalysis in vivo. BQ and 4-CB-2′,5′-HQ were administered intratracheally to male Sprague-Dawley rats (2.5 μmol/kg body weight) using nanosized polyamidoamine (PAMAM) dendrimers as carriers. We found that 24 h post application, PGE₂ metabolites in kidney of rats treated with 4-CB-2′,5′-HQ were significantly increased compared to the controls. The increase of PGE₂ metabolites was correlated with increased alveolar macrophages in lung lavage fluid. The elevation of PGE₂ synthesis is of great interest since it plays a crucial role in balancing homeostasis and inflammation where a chronic disturbance may increase risk of cancer. PAMAM dentrimers proved to be an effective transport medium and did not stimulate an inflammatory response themselves.
Show more [+] Less [-]Alleviation of lead-induced physiological, metabolic, and ultramorphological changes in leaves of upland cotton through glutathione
2016
Khan, Mumtaz | Daud, M. K. | Baṣārat Alī, Es. | Jamīl K̲h̲ān, Muḥammad | Azizullah, Azizullah | Niaz Muhammad, | Muhammad, Noor | ur Rehman, Zia | Zhu, Shui Jin
Plants face changes in leaves under lead (Pb) toxicity. Reduced glutathione (GSH) has several functions in plant metabolism, but its role in alleviating Pb toxicity in cotton leaves is still unknown. In the present study, cotton seedlings (28 days old) were exposed to 500 μM Pb and 50 μM GSH, both alone and in combination, for a period of 10 days, in the Hoagland solution under controlled growth conditions. Results revealed Pb-induced changes in cotton’s leaf morphology, photosynthesis, and oxidative metabolism. However, exogenous application of GSH restored leaf growth. GSH triggered build up of chlorophyll a, chlorophyll b, and carotenoid contents and boosted fluorescence ratios (F ᵥ/F ₘ and F ᵥ/F ₀). Moreover, GSH reduced the malondialdehyde (MDA), hydrogen peroxide (H₂O₂), and Pb contents in cotton leaves. Results further revealed that total soluble protein contents were decreased under Pb toxicity; however, exogenously applied GSH improved these contents in cotton leaves. Activities of antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), glutathione reductase (GR), and ascorbate peroxidase (APX)) were also increased by GSH application under Pb toxicity. Microscopic analysis showed that excess Pb shattered thylakoid membranes in chloroplasts. However, GSH stabilized ultrastructure of Pb-stressed cotton leaves. These findings suggested that exogenously applied GSH lessened the adverse effects of Pb and improved cotton’s tolerance to oxidative stress.
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