Environmental lead exposure has been linked with reduced kidney function. However, evidence about its role in diabetic kidney damage, especially when considering the nutritional status of vitamin D, is sparse. In this observational study, we investigated the association between low-level lead exposure and urinary albumin-to-creatinine ratio (UACR) and assessed potential impact of vitamin D among 4033 diabetic patients in Shanghai, China. Whole blood lead was measured by graphite furnace atomic absorption spectrometry. Serum 25-hydroxyvitamin D [25(OH)D] was tested using a chemiluminescence immunoassay. The associations of blood lead with UACR and albuminuria, defined as UACR ≥30 mg/g, according to 25(OH)D levels were analyzed using linear and Poisson regression models. A doubling of blood lead level was associated with a 10.7% higher UACR (95% CI, 6.19%–15.5%) in diabetic patients with 25(OH)D < 50 nmol/L, whereas the association was attenuated toward null (2.03%; 95% CI, −5.18% to 9.78%) in those with 25(OH)D ≥ 50 nmol/L. Similarly, the risk ratios of prevalent albuminuria per doubling of blood lead level between the two groups were 1.09 (95% CI, 1.03–1.15) and 0.99 (95% CI, 0.86–1.14), respectively. Joint analysis demonstrated that a combination of high blood lead and low 25(OH)D corresponded to significantly higher UACR. Among diabetic patients with 25(OH)D < 50 nmol/L, the increment of UACR relative to blood lead was more remarkable in those with reduced estimated glomerular filtration rate (<60 mL/min/1.73 m²). These results suggested that higher blood lead levels were associated with increased urinary albumin excretion in diabetic patients with vitamin D deficiency. Further prospective studies are needed to validate our findings and to determine whether vitamin D supplementation yields a benefit.

We used real-world exposure scenarios to evaluate the effect of six ambient air pollutant (PM₂.₅, PM₁₀, NO₂, SO₂, CO, and O₃) exposure on renal function among older adults without chronic kidney disease (CKD). We recruited 169 older adults without CKD in Beijing, China, for a longitudinal study from 2016 to 2018. The Modification of Diet in Renal Disease (MDRD) and the Chronic Kidney Disease Epidemiology Collaboration (EPI) equations were employed to derive the estimated glomerular filtration rate (eGFR). A linear mixed-effects model with random intercepts for participants was employed to determine the effects of air pollutants on renal function evaluated on the basis of eGFR and urinary albumin/creatinine ratio at different exposure windows (1-, 2-, 3-, 5-, 7-, 14-, 28-, 45-, and 60-days moving averages). An interquartile range (IQR) increase in NO₂ for was associated with significant decreases of in eGFR (MDRD equation) [percentage changes: −4.49 (95% confidence interval: −8.44, −0.37), −5.51 (−10.43, −0.33), −2.26 (−4.38, −0.08), −3.71 (−6.67, −0.65), −5.44 (−9.58, −1.11), −5.50 (−10.24, −0.51), −6.15 (−10.73, −1.33), and −6.34 (−11.17, −1.25) for 1-, 2-, 5-, 7-, 14-, 28-, 45-, and 60-days moving averages, respectively] and in eGFR (EPI equation) [percentage changes: −5.04 (−7.09, −2.94), −6.25 (−8.81, −3.62), −5.16 (−7.34, −2.92), −5.10 (−7.85, −2.28), −5.83 (−8.23, −3.36), −6.04 (−8.55, −3.47) for 1-, 2-, 14-, 28-, 45-, and 60-days moving averages, respectively]. In two-pollutant model, only the association of NO₂ exposure with eGFR remained robust after adjustment for any other pollutant. This association was stronger for individuals with hypertension for the EPI equation or BMI <25 kg/m² for the MDRD equation at lags 1 and 1–2. Our findings suggest that NO₂ exposure is associated with eGFR reduction among older adults without CKD for short (1-, 2-days) and medium (14-, 28-, 45-, 60-days) term exposure periods; thus, NO₂ exposure may contribute to renal impairment.

Cyclophosphamide, a common chemotherapeutic and immune suppressor agent, is regularly used in research to weaken the immune system in laboratory animal models. Nile tilapia (Oreochromis niloticus) is a widely used experimental fish model for immune-modulatory research; with the lack of knowledge about the immune-compromised tilapia model, an urgent need is to develop and optimise such a model. Sixty healthy Oreochromis niloticus fish, average weight 50 ± 10 g, were divided into four experimental groups. Fish in group I, negative control group, were injected with phosphate-buffered saline only, and fish in groups II, III and IV were injected with cyclophosphamide (CP) at 100, 200 and 400 mg/kg body weight (BW), respectively, via the intraperitoneal route. Different immune-related parameters were investigated 3 weeks after CP injection. The results have revealed a significant decrease in total red blood cells (RBCs), white blood cells (WBCs) and thrombocyte counts and reduced haemoglobin and haematocrit values in CP-treated fish, especially those injected with 200 mg/kg BW compared with the control group (p < 0.05). Also, significantly lower levels of serum proteins (total protein, albumin, α1- and γ-globulins) were observed in CP-treated fish, especially those injected with 200 mg/kg BW in comparison with the control group (p < 0.05). Furthermore, CP-treated fish showed a reduction in the expression of immune-related genes (interleukin-1, and tumour necrosis factor-α in spleen and tumour necrosis factor-α and transforming growth factor β-1 in head-kidney), especially those injected with 200 mg/kg BW compared with the control group (p < 0.05). In conclusion, the Oreochromis niloticus immune-suppressed model can be induced by intraperitoneal CP injection at 200 mg/kg BW.

PM₂.₅-related neurological and mental diseases, such as cognitive impairment and stroke, tend to cause disability. Six-week-old male C57BL/6 mice were divided into 6 groups and exposed to concentrated PM₂.₅ or filtered air for 2, 4, and 6 months, respectively. The neurobehavioral changes of mice were tested. The weight of the whole brain and olfactory bulbs were recorded at the end of exposure, and the brain structure was observed by hematoxylin and eosin (HE) staining. Serum indicators, mRNA, and protein expressions were detected. The spatial learning memory ability was impaired, and the mice were more anxious after PM₂.₅ exposure. Relative brain weight decreased with age, and PM₂.₅ exposure exceeded the decrease of relative brain weight. Interestingly, superoxide dismutase (SOD) and albumin decreased in the PM₂.₅-exposed groups although neuronal morphology and other serum indicators did not show significant difference between PM and FA groups. Moreover, PM₂.₅ induced the increase of plasminogen at 2 months but recovered at 4 months and then increased at 6 months again. The results from protein expression and transcriptomic test demonstrated that PI3K/AKT/FoxO1 pathway might be activated after 6-month PM₂.₅ exposure in mice. Indicators albumin, the percentage of albumin over IgG (A/G value), and plasminogen were the main serous changes in mice after early-stage (2 months) and long-term (6 months) PM₂.₅ exposure. In addition, early-stage injury induced by PM₂.₅ might recover at later time point and display significant injury again with the exposure time. PM₂.₅ exposure-induced brain injury might be associated with the activation of PI3K/AKT/FoxO1 pathway.

The accumulation of microplastics (MPs) is a growing problem in aquatic ecosystems. Despite increased research on MPs in the last decade, their potential threat to freshwater ecosystems remains an open question. In the present study, the negative impacts of MPs were investigated on blood biochemical parameters in the European pond turtle (Emys orbicularis). Pond turtles were distributed into three experimental groups (n = 9 for each group) and were fed diets containing 250, 500, and 1000 mg MPs (PE100 polyethylene) per kg of food for 30 days, and a control group fed with a standard uncontaminated diet. The results indicated that exposure to 500 and 1000 mg kg⁻¹ MPs caused a significant increase in the activities of alanine and aspartate aminotransferases, and in the levels of cholesterol, glucose, creatinine, urea, and calcium (Ca⁺²) compared with the control group. On the contrary, the activity of gamma-glutamyl transferase and the levels of total protein, albumin, total immunoglobulins, and phosphorus were significantly reduced in E. orbicularis exposed to 500 and 1000 mg kg⁻¹ MPs when compared with the controls. In all the MP-exposed groups, the activity of lactate dehydrogenase and globulin and magnesium (Mg⁺²) levels were significantly reduced; while creatine phosphokinase and alkaline phosphatase activities were increased with respect to the control turtles. A significant decrease in triglyceride levels was reported in E. orbicularis exposed to 1000 mg kg⁻¹ MPs. MPs intake induced notable alterations in blood biochemical parameters of E. orbicularis. These results suggest that changes in the blood biochemical parameters could be an appropriate bio-indicator to evidence the existence of tissue damage in E. orbicularis.

In 2019, PM₂.₅ and PM₁.₀ samples were collected in Harbin City, Heilongjiang Province, China, to research their mass concentration, number concentration, metal composition analysis, impact on lung injury of mice, and metal source analysis. The results showed that the mass concentration of PM₂.₅ and PM₁.₀ in the whole year of 2019 showed a trend of high in winter and low in summer. The mass concentration of PM₁.₀ was 62~85% of the total mass concentration of PM₂.₅, and the changing trend of PM₁.₀ number concentration was lower than that of the two sides. Its size was negatively correlated with the temperature and no significant correlation with the relative humidity. The analysis of metal composition found that the emission of coal and motor vehicle exhaust increased the concentration of Zn, Pb, As, and Cu in PM₁.₀. And setting off fireworks and firecrackers during the Spring Festival caused the concentration of Ti, Al, and Mg to increase. The burning of biomass also made the K concentration higher in autumn and winter than the other two-quarters. PMF analysis showed that coal combustion, soil dust and vehicle exhaust were the main factors of PM₁.₀ pollution in winter. Electron microscope scanning showed that PM₁.₀ particle morphology in winter was mainly flocculent aggregated particles generated by coal combustion. In the study of the effects of atmospheric particulate matter on lung injury in mice, it was found that lactate dehydrogenase (LDH), acid phosphatase (ACP),alkaline phosphatase (AKP), and albumin (ALB) in lung tissue cells of mice exposed to air particulates increased, indicating that PM₂.₅ and PM₁.₀ had toxic effects on lung tissue cells of mice. The level of serum malondialdehyde (MDA), nitric oxide (NO), and nitric oxide synthase (NOS) in lung lavage fluid increased, and the activity of superoxide dismutase (SOD) decreased, which indicated that both PM₂.₅ and PM₁.₀ could cause oxidative damage in the body, and with the increase of the concentration of PM₂.₅ and PM₁.₀ in the air particles, the cytotoxic effect on the lung tissue of mice was enhanced and the degree of oxidative damage was increased.

The present study has been conducted to evaluate the potential threat of NiO nanoparticles (NiO NPs) on an edible fish Heteropneustes fossilis. Fishes selected for the study were exposed to four concentrations of NiO NPs (12, 24, 36 and 48 mg/l) for the period of 14 days, and various haematological, biochemical and enzymological changes in the exposed fishes were examined. Results revealed that maximum fluctuations were seen in 48-mg/l-exposed fishes when compared with the control in terms of the haematological parameters (RBC count, WBC count, Hb content, Ht% and O₂ carrying capacity of blood), enzymatic activities (AST, ALP, ALT and LDH) and biochemical parameters (level of cholesterol, triglycerides, glucose, total protein, albumin, globulin, bilirubin and creatinine). However, 12 mg/l treatment to the fishes showed its least impact on aforesaid parameters. Furthermore, Ni accumulation and changes in cortisol level in the blood were also noticed in all the treated fishes. Structural changes, such as membrane and nuclear disintegration, micronucleus, deformed and vacuolated cells, and enucleation were also observed in RBCs of NiO NP–treated fishes. Conclusively, our study provides useful information and insight for the possible ecotoxicity of NiO NPs on aquatic organisms and emphasizes upon the importance of treatment of effluents containing nanoparticles before their release into the aquatic system.

This study examines the potential defending effects of the diatom, Amphora coffeaeformis, as a feed additive versus the deleterious effects (mainly on gonads) caused by microplastics (MPs) in Nile tilapia, Oreochromes niloticus. Groups of male tilapia were pre-fed diets with four different supplementation levels of A. coffeaeformis (0%, 2.5%, 5%, and 7.5%) for 70 days, then exposed to 10 mg/L MPs for 15 days. Thereafter, samples were taken from the four experimental groups and the control fish group, for evaluating blood picture, erythrocytes alterations, biochemical parameters, catalase (CAT), superoxide dismutase (SDO), and total antioxidant capacity (TAC). In addition, male reproductive performance was assessed by quantifying the follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T), also testicular sections and GSI% were also assessed. The results showed that the number of leucocytes were significantly (p < 0.05) higher, but the number of red blood cells (RBCs), the level of Hemoglobin (Hb), the percentage of Hematocrit (Ht), the number of platelets, and the eosinophil percentages were significantly decreased (p < 0.05) when fish exposed to MPs. Biochemical parameters (ALP, glucose, uric acid, albumin, and A/G ratio) were significantly increased after MP exposure compared with the control group. Furthermore, MPs induced a significant decline in both serum LH and T levels. Testicular, histological, degenerative changes and testis-ova were found in the MP-exposed fish. Thus, A. coffeaeformis supplementation displayed ameliorative properties that detoxified the negative effects of MPs. This study provides a better understanding of the reproductive injuries caused by MPs exposure and evidence for the use of A. coffeaeformis as a natural remedy in freshwater tilapia.

Silver nanoparticles (Ag NPs) have wide medical and industrial applications; therefore, their release into aquatic environments is a problematic issue. The present study aims to evaluate the removal efficiency of Ag NPs from water using orange peel (OP) and banana peel (BP) to moderate their toxicity on Oreochromis niloticus. Fish were divided into 4 groups: control group (dechlorinated tap water), Ag NPs (4 mg/L) exposed group, Ag NPs (4 mg/L) + OP (40 mg/L) group, and Ag NPs (4 mg/L) + BP (40 mg/L) group for 24 h, 48 h, and 96 h. The adsorptive ability of both peels was confirmed by scanning electron microscope and energy-dispersive X-ray spectroscopy after the exposure processes. The biochemical results revealed a gradual elevation in plasma glucose, total proteins, globulin, liver enzymes (AST, ALT, and ALP), creatinine, and uric acid after Ag NPs exposure, while albumin and total lipid concentrations were significantly decreased. The recorded antioxidant biomarkers in gills, and liver tissues after Ag NPs exposure showed severe oxidative damages (maximally after 96 h) as indicated by marked elevations in thiobarbituric acid reactive substances, glutathione peroxidase, catalase, and superoxide dismutase values, and decreased glutathione reduced content. All studied parameters restored more or less to that of control groups after OP and BP water treatment. The adsorbent abilities of both peels could reduce Ag NPs bioavailability and moderate their toxicological impacts.

The organophosphate (OP) pesticides are neurotoxic compounds widely used around the world. Evaluation of OP exposure in human studies is important for enabling adequate data analyses and drawing accurate conclusions. The aim of this study was to analyze OP exposure biomarkers and their relationships in a Mexican population with different exposure levels. Dialkylphosphates (DAP) were determined through gas chromatography-mass spectrometry (GC-MSD); acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), arylesterase (AREase), basal paraoxonase 1 (PONase), and β-glucuronidase activities were detected using spectrophotometric methods. The albumin content was determined in a certified clinical laboratory. The DMTP metabolite was found in the highest concentration, and a negative and significant correlation between DAP and cholinesterase activity was observed. Our results suggested that BuChE is a considerably more sensitive biomarker than AChE. In addition, β-glucuronidase was positively correlated with albumin, BuChE, and PONase. In conclusion, our data strongly support the use of two or more biomarkers of exposure in human monitoring and the application of a strong and validated questionnaire.