Seagrasses, marine flowering plants, provide a wide range of ecosystem services, defined here as natural processes and components that directly or indirectly benefit human needs. Recent research has shown that there are still many gaps in our comprehension of seagrass ecosystem service provision. Furthermore, there seems to be little public knowledge of seagrasses in general and the benefits they provide. This begs the questions: how do we move forward with the information we have? What other information do we need and what actions do we need to take in order to improve the situation and appreciation for seagrass? Based on the outcomes from an international expert knowledge eliciting workshop, three key areas to advance seagrass ecosystem service research were identified: 1) Variability of ecosystem services within seagrass meadows and among different meadows; 2) Seagrass ecosystem services in relation to, and their connection with, other coastal habitats; and 3) Improvement in the communication of seagrass ecosystem services to the public. Here we present ways forward to advance seagrass ecosystem service research in order to raise the profile of seagrass globally, as a means to establish more effective conservation and restoration of these important coastal habitats around the world.

Eutrophication causes the major impact in the coastal waters of the state of Yucatan. In general, loss of water quality and biological communities and massive development of toxic microorganisms are some of the consequences of this phenomenon. To reveal changes in species composition and cell abundance of the taxocoenosis of epibenthic dinoflagellates before and after a harmful algal bloom event in the water column that lasted about 150days (August–December 2011) in the Dzilam – San Crisanto area (northern Yucatan Peninsula, southeastern Gulf of Mexico) were the main objectives of the present study. In August 2011 and September 2012, sampling along 20 transects perpendicular to the coastline along the entire northern Yucatan coast, starting from 20 sampling sites from El Cuyo in the east to Celestún in the west, at a distance of 50, 150 and 250m from the coast, was carried out. Physicochemical characteristics measured before and after the bloom were within the ranges previously reported in the study area. Salinity was the most stable characteristic, with mean values of 36.25 and 36.42 in 2011 and 2012, respectively. Phosphates were the only parameter that showed a wide range with higher values before the bloom (0.03–0.54μM/l). A total of 168 macrophyte (seaweeds and seagrasses), sponge and sediment samples (105 in 2011 and 63 in 2012) that included associated microphytobenthos were taken by snorkeling from 0.7 to 5m depth. Six substrate types were distinguished: Chlorophyta, Phaeophyceae, Rhodophyta, Angiospermae (seagrasses), Demospongiae (sponges) and sediment. Chlorophytes dominated the collected samples: 38 samples in 2011 and 23 in 2012. Avrainvillea longicaulis f. laxa predominated before the bloom and Udotea flabellum after it. In total, 25 epibenthic dinoflagellate species from 11 genera were found. The genus Prorocentrum was the most representative in terms of the number of species. The highest total dinoflagellate cell abundances were observed in the sites with different types of macrophytes (up to 2441cells/g substrate wet weight in 2011 and up to 1068cells/g in 2012). The lowest cell densities were observed in the areas with scarce or no macrophytes on sandy seafloor. Before the bloom, Prorocentrum rhathymum (up to 4995cells/g) and P. cf. sipadanensis (up to 5275cells/g) were the most abundant, and after the bloom the latter was dominant (up to 3559cells/g); in 2012, both variety of substrates and dinoflagellate cell abundance diminished. A canonical correspondence analysis revealed significant relationships between the physicochemical variables and epiphytic/benthic dinoflagellate species either before or after the bloom. The pelagic bloom resulted in the loss of substrate for epiphytic dinoflagellates, which caused replacement of the dominant species and a decrease in cell abundance of the whole taxocoenosis.

Contaminant concentrations are reported for surface water, sediment, flora and fauna collected during 2010–2011 from the mangrove fringe along eastern Tampa Bay, Florida. Concentrations of trace metals, chlorinated pesticides, atrazine, total polycyclic aromatic hydrocarbons, and polychlorinated biphenyls were species-, chemical- and location-specific. Contaminants in sediments did not exceed proposed individual sediment quality guidelines. Most sediment quality assessment quotients were less than one indicating the likelihood of no inhibitory effect based on chemical measurements alone. Faunal species typically contained more contaminants than plant species; seagrass usually contained more chemicals than mangroves. Bioconcentration factors for marine angiosperms were usually less than 10 and ranged between 1 and 31. Mercury concentrations (ppm) in blue crabs and fish did not exceed the U.S. Environmental Protection Agency fish tissue criterion of 0.3 and the U.S. Food and Drug Administration action level of 1.0. In contrast, total mercury concentrations in faunal species often exceeded guideline values for wildlife consumers of aquatic biota.

The use of low-polarity organic solvents is widespread in cleanup/extraction processes in order to carry compounds of interest, remove interferences and separate phases, among other uses. A large number of studies have used delipidation to remove excess of lipids to analyse carbon stable isotopes in biological tissues for trophic and behavioural ecology investigations. In this context, the primary aim of this study is to assess the influence of one delipidation process on the results of total mercury (Hg) analyses and the possible use of delipidated samples from previous analyses, such as for stable isotopes, in Hg level determination. Samples of vegetation (angiosperm, lichens and mosses), invertebrates (krill and limpets), fish (marbled and black rockcod), bird liver and eggs (Antarctic, Gentoo and Adélie penguins, kelp gull, Antarctic tern, cape petrel and giant southern petrel) and pinniped tissues (Weddell seal, crabeater seal, southern elephant seal and Antarctic fur seal) were analysed for Hg before and after delipidation by cyclohexane. The difference between the two measurements ranged individually from −63 to 136% (in the moss Sanionia uncinata) and the averages ranged from −60 to 66% (in pinniped tissues). The proportion of organic Hg, which presents considerable lipophilicity, but also high affinity for sulfhydryl groups in proteins, might be responsible for such variability. Given the limitations of our study, we think it is safe to say that delipidated samples could not be used to infer total Hg values in non-delipidated ones.

Pyrrolizidine alkaloids (PAs) and related N-oxides (PANOs) are secondary plant metabolites thought to be found in approximately 3% of the flowering plants worldwide and exhibiting hepatotoxic properties to humans. As a consequence, beehive products are prone to be contaminated with those compounds by bees foraging PA-producing plants. Downstream contamination can also occur through food items containing honey. Analytical methods based on UHPLC separation and MS/MS detection were developed with a focus on very low LOQs and validated for the analysis of 16 PAs and 14 PANOs in honey, honey-based candies and snacks, as well as beehive product–based food supplements. A maximum level of 182 ng/g of PAs was detected in a Mediterranean honey, and high levels of heliotrine-type compounds were reported for the first time. An extensive sampling of honeys harvested in Belgium was performed (N = 374), the concentration levels were more limited with a maximum of 60 ng/g, and the contamination pattern was dominated by senecionine-type PAs. The PA levels in honey-based candies and snacks were very low, with respective maxima of 7.61 ng/g and 0.36 ng/g. Seventy-five percent among the pre-dosed food supplements based on beehive products were contaminated, with a maximum of 43 ng/g. The highest level was detected in a bee-collected pollen sample (1672 ng/g). The analytical results were consistent with the previously reported data for beehive products and confirmed that PA/PANO contamination in these food commodities is recurrent.