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Responses to herbicides of Arctic and temperate microalgae grown under different light intensities
2023
Du, Juan | Izquierdo, Disney | Xu, Hai-feng | Beisner, Beatrix | Lavaud, Johann | Ohlund, Leanne | Sleno, Lekha | Juneau, Philippe
In aquatic ecosystems, microalgae are exposed to light fluctuations at different frequencies due to daily and seasonal changes. Although concentrations of herbicides are lower in Arctic than in temperate regions, atrazine and simazine, are increasingly found in northern aquatic systems because of long-distance aerial dispersal of widespread applications in the south and antifouling biocides used on ships. The toxic effects of atrazine on temperate microalgae are well documented, but very little is known about their effects on Arctic marine microalgae in relation to their temperate counterparts after light adaptation to variable light intensities. We therefore investigated the impacts of atrazine and simazine on photosynthetic activity, PSII energy fluxes, pigment content, photoprotective ability (NPQ), and reactive oxygen species (ROS) content under three light intensities. The goal was to better understand differences in physiological responses to light fluctuations between Arctic and temperate microalgae and to determine how these different characteristics affect their responses to herbicides. The Arctic diatom Chaetoceros showed stronger light adaptation capacity than the Arctic green algae Micromonas. Atrazine and simazine inhibited the growth and photosynthetic electron transport, affected the pigment content, and disturbed the energy balance between light absorption and utilization. As a result, during high light adaptation and in the presence of herbicides, photoprotective pigments were synthesized and NPQ was highly activated. Nevertheless, these protective responses were insufficient to prevent oxidative damage caused by herbicides in both species from both regions, but at different extent depending on the species. Our study demonstrates that light is important in regulating herbicide toxicity in both Arctic and temperate microalgal strains. Moreover, eco-physiological differences in light responses are likely to support changes in the algal community, especially as the Arctic ocean becomes more polluted and bright with continued human impacts.
Show more [+] Less [-]Integrated Method of Ozonation and Anaerobic Process for Treatment of Atrazine bearing Wastewater
2023
Saba Khurshid, Abdur Rahman Quaff and Ramakar Jha
The paper presents the treatment of atrazine-contaminated wastewater by ozonation followed by an anaerobic process using Upflow Anaerobic Sludge Blanket (UASB) reactor. The experiment was performed with 100 ppb synthetic solutions of atrazine prepared in ultra-pure water. The corresponding initial Chemical Oxygen Demand (COD) is 226 mg.L-1. The initial pH was adjusted to 9.5. The atrazine-bearing synthetic wastewater was ozonated with an ozone dose of 9.4mg/l for 40 minutes of optimum ozonation time, resulting in a 35% reduction in the initial concentration of atrazine. Along with atrazine reduction, there was a COD removal of 54.42%. Further, it was degraded with an anaerobic process, resulting in the final reduction in atrazine concentration of 81% and the corresponding removal in COD of 86.7%. The process of ozonation led to the mineralization of atrazine and enhancement in the biodegradability of the wastewater. Using ion chromatography, the ozonated wastewater sample was analyzed for ionic by-products before and after ozonation. The ion chromatography results showed the breaking of the atrazine compound and the formation of Cl-, NO3-, SO42-, and F- as intermediate products. Further, the BOD5/COD ratio increased, reflecting the increased biodegradability. This ozonated wastewater was treated in a UASB reactor where the pesticide was degraded to 19 ppb, and COs degraded to 30 mg.L-1. The overall removal of atrazine pesticide and COD were 81% and 86.7%, respectively, in the integrated system of ozonation followed by anaerobic degradation.
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