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Titanium dioxide nanoparticles enhance mortality of fish exposed to bacterial pathogens
2015
Jovanović, Boris | Whitley, Elizabeth M. | Kimura, Kayoko | Crumpton, Adam | Palić, Dušan
Nano-TiO2 is immunotoxic to fish and reduces the bactericidal function of fish neutrophils. Here, fathead minnows (Pimephales promelas) were exposed to low and high environmentally relevant concentration of nano-TiO2 (2 ng g−1 and 10 μg g−1 body weight, respectively), and were challenged with common fish bacterial pathogens, Aeromonas hydrophila or Edwardsiella ictaluri. Pre-exposure to nano-TiO2 significantly increased fish mortality during bacterial challenge. Nano-TiO2 concentrated in the kidney and spleen. Phagocytosis assay demonstrated that nano-TiO2 has the ability to diminish neutrophil phagocytosis of A. hydrophila. Fish injected with TiO2 nanoparticles displayed significant histopathology when compared to control fish. The interplay between nanoparticle exposure, immune system, histopathology, and infectious disease pathogenesis in any animal model has not been described before. By modulating fish immune responses and interfering with resistance to bacterial pathogens, manufactured nano-TiO2 has the potential to affect fish survival in a disease outbreak.
Show more [+] Less [-]Arsenate reduction and mobilization in the presence of indigenous aerobic bacteria obtained from high arsenic aquifers of the Hetao basin, Inner Mongolia
2015
Guo, Huaming | Liu, Zeyun | Ding, Susu | Hao, Chunbo | Xiu, Wei | Hou, Weiguo
Intact aquifer sediments were collected to obtain As-resistant bacteria from the Hetao basin. Two strains of aerobic As-resistant bacteria (Pseudomonas sp. M17-1 and Bacillus sp. M17-15) were isolated from the aquifer sediments. Those strains exhibited high resistances to both As(III) and As(V). Results showed that both strains had arr and ars genes, and led to reduction of dissolved As(V), goethite-adsorbed As(V), scorodite As(V) and sediment As(V), in the presence of organic carbon as the carbon source. After reduction of solid As(V), As release was observed from the solids to solutions. Strain M17-15 had a higher ability than strain M17-1 in reducing As(V) and promoting the release of As. These results suggested that the strains would mediate As(V) reduction to As(III), and thereafter release As(III), due to the higher mobility of As(III) in most aquifer systems. The processes would play an important role in genesis of high As groundwater.
Show more [+] Less [-]Predicting fecal indicator organism contamination in Oregon coastal streams
2015
Pettus, Paul | Foster, Eugene | Pan, Yangdong
In this study, we used publicly available GIS layers and statistical tree-based modeling (CART and Random Forest) to predict pathogen indicator counts at a regional scale using 88 spatially explicit landscape predictors and 6657 samples from non-estuarine streams in the Oregon Coast Range. A total of 532 frequently sampled sites were parsed down to 93 pathogen sampling sites to control for spatial and temporal biases. This model's 56.5% explanation of variance, was comparable to other regional models, while still including a large number of variables. Analysis showed the most important predictors on bacteria counts to be: forest and natural riparian zones, cattle related activities, and urban land uses. This research confirmed linkages to anthropogenic activities, with the research prediction mapping showing increased bacteria counts in agricultural and urban land use areas and lower counts with more natural riparian conditions.
Show more [+] Less [-]Impact of manufactured TiO2 nanoparticles on planktonic and sessile bacterial communities
2015
Jomini, Stéphane | Clivot, Hugues | Bauda, Pascale | Pagnout, Christophe
In the present study, we conducted a 2 week microcosm experiment with a natural freshwater bacterial community to assess the effects of titanium dioxide nanoparticles (TiO2-NPs) at various concentrations (0, 1, 10 and 100 mg/L) on planktonic and sessile bacteria under dark conditions. Results showed an increase of planktonic bacterial abundance at the highest TiO2-NP concentration, concomitant with a decrease from that of sessile bacteria. Bacterial assemblages were most affected by the 100 mg/L TiO2-NP exposure and overall diversity was found to be lower for planktonic bacteria and higher for sessile bacteria at this concentration. In both compartments, a 100 mg/L TiO2-NPs exposure induced a decrease in the ratio between the Betaproteobacteria and Bacteroidetes. For planktonic communities, a decrease of Comamonadaceae was observed concomitant with an increase of Oxalobacteraceae and Cytophagaceae (especially Emticicia). For sessile communities, results showed a strong decrease of Betaproteobacteria and particularly of Comamonadaceae.
Show more [+] Less [-]Ecotoxicogenomic assessment of diclofenac toxicity in soil
2015
Chen, Guangquan | den Braver, Michiel W. | van Gestel, Cornelis A.M. | Straalen, N. M. van | Roelofs, Dick
Diclofenac is widely used as nonsteroidal anti-inflammatory drug leaving residues in the environment. To investigate effects on terrestrial ecosystems, we measured dissipation rate in soil and investigated ecotoxicological and transcriptome-wide responses in Folsomia candida. Exposure for 4 weeks to diclofenac reduced both survival and reproduction of F. candida in a dose-dependent manner. At concentrations ≥200 mg/kg soil diclofenac remained stable in the soil during a 21-day incubation period. Microarrays examined transcriptional changes at low and high diclofenac exposure concentrations. The results indicated that development and growth were severely hampered and immunity-related genes, mainly directed against bacteria and fungi, were significantly up-regulated. Furthermore, neural metabolic processes were significantly affected only at the high concentration. We conclude that diclofenac is toxic to non-target soil invertebrates, although its mode of action is different from the mammalian toxicity. The genetic markers proposed in this study may be promising early markers for diclofenac ecotoxicity.
Show more [+] Less [-]Heteroaggregation, transformation and fate of CeO2 nanoparticles in wastewater treatment
2015
Barton, Lauren E. | Auffan, Melanie | Olivi, Luca | Bottero, Jean-Yves | Wiesner, Mark R.
Wastewater Treatment Plants (WWTPs) are a key pathway by which nanoparticles (NPs) enter the environment following release from NP-enabled products. This work considers the fate and exposure of CeO2 NPs in WWTPs in a two-step process of heteroaggregation with bacteria followed by the subsequent reduction of Ce(IV) to Ce(III). Measurements of NP association with solids in sludge were combined with experimental estimates of reduction rate constants for CeO2 NPs in Monte Carlo simulations to predict the concentrations and speciation of Ce in WWTP effluents and biosolids. Experiments indicated preferential accumulation of CeO2 NPs in biosolids where reductive transformation would occur. Surface functionalization was observed to impact both the distribution coefficient and the rates of transformation. The relative affinity of CeO2 NPs for bacterial suspensions in sludge appears to explain differences in the observed rates of Ce reduction for the two types of CeO2 NPs studied.
Show more [+] Less [-]Effects of organic pollution on biological communities of marine biofilm on hard substrata
2015
Sanz-Lázaro, C. | Fodelianakis, S. | Guerrero-Meseguer, L. | Marin, A. | Karakassis, I.
We examined the effect of organic enrichment on diatom and bacterial assemblages of marine epilithic biofilms on two locations in the Mediterranean, one situated in Spain and the other in Greece. Total organic carbon, total organic nitrogen, stable isotopes (δ13C and δ15N) and chlorophyll a indicated significant incorporation of organic wastes, increased primary production and trophic niche modifications on the biofilms close to the organic enrichment source. In Spain, where the organic load was higher than in Greece, diatom and, to some extent, bacterial assemblages varied following the organic enrichment gradient. The taxonomic richness of diatom and bacterial communities was not influenced by organic enrichment. Classical community parameters showed consistent patterns to organic pollution in both locations, whereas community assemblages were only influenced when organic pollution was greatest. The successional patterns of these communities were similar to other epilithic communities. The modification of community assemblages induced by organic pollution may affect ecological functions.
Show more [+] Less [-]Bioremediation of 1,2-dichloroethane contaminated groundwater: Microcosm and microbial diversity studies
2015
Wang, S.Y. | Kuo, Y.C. | Huang, Y.Z. | Huang, C.W. | Kao, C.M.
In this study, the effectiveness of bioremediating 1,2-dichloroethane (DCA)-contaminated groundwater under different oxidation–reduction processes was evaluated. Microcosms were constructed using indigenous bacteria and activated sludge as the inocula and cane molasses and a slow polycolloid-releasing substrate (SPRS) as the primary substrates. Complete DCA removal was obtained within 30 days under aerobic and reductive dechlorinating conditions. In anaerobic microcosms with sludge and substrate addition, chloroethane, vinyl chloride, and ethene were produced. The microbial communities and DCA-degrading bacteria in microcosms were characterized by 16S rRNA-based denatured-gradient-gel electrophoresis profiling and nucleotide sequence analyses. Real-time polymerase chain reaction was applied to evaluate the variations in Dehalococcoides spp. and Desulfitobacterium spp. Increase in Desulfitobacterium spp. indicates that the growth of Desulfitobacterium might be induced by DCA. Results indicate that DCA could be used as the primary substrate under aerobic conditions. The increased ethene concentrations imply that dihaloelimination was the dominate mechanism for DCA biodegradation.
Show more [+] Less [-]Biotransformation of arsenite and bacterial aox activity in drinking water produced from surface water of floating houses: Arsenic contamination in Cambodia
2015
Chang, Jin-Soo
The potential arsenite bioteansformation activity of arsenic was investigated by examining bacterial arsenic arsenite-oxidizing gene such as aoxS, aoxR, aoxA, aoxB, aoxC, and aoxD in high arsenic-contaminated drinking water produced from the surface water of floating houses. There is a biogeochemical cycle of activity involving arsenite oxidase aox system and the ars (arsenic resistance system) gene operon and aoxR leader gene activity in Alcaligenes faecalis SRR-11 and aoxS leader gene activity in Achromobacter xylosoxidans TSL-66. Batch experiments showed that SRR-11 and TSL-66 completely oxidized 1 mM of As (III) to As (V) within 35–40 h. The leaders of aoxS and aoxR are important for gene activity, and their effects in arsenic bioremediation and mobility in natural water has a significant ecological role because it allows arsenite oxidase in bacteria to control the biogeochemical cycle of arsenic-contaminated drinking water produced from surface water of floating houses.
Show more [+] Less [-]Dual partitioning and attachment effects of rhamnolipid on pyrene biodegradation under bioavailability restrictions
2015
Congiu, Eleonora | Parsons, John R. | Ortega-Calvo, Jose Julio
We investigated the effects of different bioavailability scenarios on the rhamnolipid-enhanced biodegradation of pyrene by the representative polycyclic aromatic hydrocarbon degrader Mycobacterium gilvum VM552. This biosurfactant enhanced biodegradation when pyrene was provided in the form of solid crystals; no effect was observed when the same amount of the chemical was preloaded on polydimethylsiloxane (PDMS). An enhanced effect was observed when pyrene was sorbed into soil but not with the dissolved compound. Synchronous fluorescence spectrophotometry and liquid scintillation were used to determine the phase exchange of pyrene. We also investigated the phase distribution of bacteria. Our results suggest that the rhamnolipid can enhance the biodegradation of pyrene by micellar solubilization and increase diffusive uptake. These mechanisms increase substrate acquisition by bacterial cells at exposure concentrations well above the half-saturation constant for active uptake. The moderate solubilization of pyrene from PDMS by the rhamnolipid and the prevention of cell attachment may explain the lack of enhancement for pyrene-preloaded PDMS.
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