Moss (as a reference material) and camphor (Cinnamomum Camphora) leaf, branch bark and bark samples were systematically collected across an urban-rural gradient in Guiyang (SW China) to determine the efficacy of using these bio-indicators to evaluate nitrogen (N) pollution. The tissue N concentrations (0.13%–2.70%) and δ¹⁵N values (−7.5‰ to +9.3‰) of all of these bio-indicators exhibited large spatial variations, as they recorded higher values in urban areas that quickly decreased with distance from the city center; moreover, both soil N concentrations and soil δ¹⁵N values were found no significant differences within each 6 km from the urban to the rural area. This not only suggests that the different N uptake strategies and variety of N responses of these bio-indicators can be reflected by their different susceptibilities to variations in N deposition but also reveals that they are able to indicate that urban N deposition is mostly from traffic and industry (NOₓ-N), whereas rural N deposition is mainly from agriculture (NHₓ-N). Compared to previously collected urban moss and camphor leaf samples, the significantly increased δ¹⁵N values in current urban moss and camphor leaf samples further indicate a greater contribution of NOₓ-N than NHₓ-N to urban N deposition. The feasibility of using the N concentrations and δ¹⁵N values of branch bark and bark as biomarkers of N deposition thus was further confirmed through the comparative use of these bio-indicators. It can be concluded that vascular plant leaves, branch bark and bark can be used as useful biomonitoring tools for evaluating atmospheric N pollution. For further study, quantitative criteria for the practical use of these bio-indicators in response to N deposition should be developed and the differences in the δ¹⁵N values of different plant parts should also be considered, particularly in urban environments that are severely disrupted by atmospheric pollution.

Natural levels of heavy metals (HM) have increased during the industrial era to the point of posing a serious threat to the environment. The use of tree species to record contamination is a well-known practice. The objective of the study was to compare HM levels under different pollution conditions: a) soil pollution due to mining waste; b) atmospheric pollution due to coal-fired power plant emissions. We report significant HM enrichment in Pinus halepensis tissues. Near a burning power plant, Pb content in a tree wood was 2.5-fold higher that in natural areas (no pollution; NP). In mining areas, Cd content was 25-fold higher than NP. The hypothesis that HM contents in tree rings should register pollution is debatable. HM uptake by pines from soil, detoxification mechanisms and resuspended local soil dust is involved in HM contents in wood and bark.

A national citizen survey quantified the abundance of epiphytic lichens that are known to be either sensitive or tolerant to nitrogen (N) deposition. Records were collected across the UK from over 10,000 individual trees of 22 deciduous species. Mean abundance of tolerant and sensitive lichens was related to mean N deposition rates and climatic variables at a 5 km scale, and the response of lichens was compared on the three most common trees (Quercus, Fraxinus and Acer) and by assigning all 22 tree species to three bark pH groups. The abundance of N-sensitive lichens on trunks decreased with increasing total N deposition, while that of N-tolerant lichens increased. The abundance of N-sensitive lichens on trunks was reduced close to a busy road, while the abundance of N-tolerant lichens increased. The abundance of N-tolerant lichen species on trunks was lower on Quercus and other low bark pH species, but the abundance of N-sensitive lichens was similar on different tree species. Lichen abundance relationships with total N deposition did not differ between tree species or bark pH groups. The response of N-sensitive lichens to reduced nitrogen was greater than to oxidised N, and the response of N-tolerant lichens was greater to oxidised N than to reduced N. There were differences in the response of N-sensitive and N-tolerant lichens to rainfall, humidity and temperature. Relationships with N deposition and climatic variables were similar for lichen presence on twigs as for lichen abundance on trunks, but N-sensitive lichens increased, rather than decreased, on twigs of Quercus/low bark pH species. The results demonstrate the unique power of citizen science to detect and quantify the air pollution impacts over a wide geographical range, and specifically to contribute to understanding of lichen responses to different chemical forms of N deposition, local pollution sources and bark chemistry.

Biochar as a filtering media has been attracted increasing attention for applications in urban storm-water runoff (USWR) management. Up-flow percolation tests were conducted with pine bark (PB) and biochars from PB (BCPB) for evaluating changes in dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) concentrations varying with pyrolysis temperatures (i.e., 300, 450, and 700 °C) and types of USWR (i.e., roof and pathway USWR). The most suitable pyrolysis temperature for limiting DOC leaching from BCPB depends on the types of USWR. For all the adopted pyrolysis temperature, BCPB released cumulative amount of DOC up to 0.01% of the TC content in the up-flow percolation tests with pathway USWR. High-temperature (i.e., 700 °C) BCPB released lower cumulative amount of DOC (up to 0.02% of the TC content) compared to the low-temperature (< 450 °C) BCPB in the roof USWR up-flow percolation tests. As for BCPB effectiveness in carbon sequestration, the amount of carbon that is not retained in BCPB because of leaching (DOC less than 0.1% of the TC content) may be considered negligible. Of the tested BCPB, only high-temperature BCPB removed TDN for both the types of USWR with cumulative removed quantities up to 0.006 g/kg dry weight, thus representing a better option for reducing N loads to receiving water basins.

Low methane (CH₄) emission reduction efficiency (< 25%) has been prevalent due to inefficient biological exhaust gas treatment facilities in mechanic biological waste treatment plants (MBTs) in Germany. This study aimed to quantify the improved capacity of biofilters composed of a mixture of organic (pine bark) and inorganic (expanded clay) packing materials in reducing CH₄ emissions in both a lab-scale experiment and field-scale implementation. CH₄ removal performance was evaluated using lab-scale biofilter columns under varied inflow CH₄ concentrations (70, 130, and 200 g m⁻³) and corresponding loading rates of 8.2, 4.76, and 3.81 g m⁻³ h⁻¹, respectively. The laboratory CH₄ removal rates (1.2–2.2 g m⁻³ h⁻¹) showed positive correlation with the inflow CH₄ loading rates (4–8.2 g m⁻³ h⁻¹), indicating high potential for field-scale implementation. Three field-scale biofilter systems with the proposed mixture packing materials were constructed in an MBT in Neumünster, northern Germany. A relatively stable CH₄ removal efficiency of 38–50% was observed under varied inflow CH₄ concentrations of 28–39 g m⁻³ (loading rates of 1120–2340 g m⁻³ h⁻¹) over a 24-h period. The CH₄ removal rate was approximately 500–700 g m⁻³ h⁻¹, which was significantly higher than relevant previously reported field-scale biofilter systems (16–50 g m⁻³ h⁻¹). The present study provides a promising configuration of biofilter systems composed of a mixture of organic (pine bark) and inorganic (expanded clay) packing materials to achieve high CH₄ emission reduction. Graphic abstract ᅟ

Biofilters have been shown to be efficient for removing pollutants from different water effluents, but little information is available about their capacity to remove highly polar pesticides from agricultural run-off waters. In this study, we assess the capacity of three different biofilter-supporting materials (sand, peat soil, and pine bark) to remove five phenoxyacid herbicides (mecoprop, dicamba, MCPA, dichlorprop and 2,4-D) and five non-ionic pesticides (atrazine, simazine, fenitrotion, diazinon, and alachlor) from real agricultural run-off waters. The experimental design included three columns 120 cm in length and 15 cm in diameter, each filled with 100 cm of one of the selected supporting materials. After 30 days of acclimation, the columns were fed with agricultural run-off water spiked at 10 μg L⁻¹ with each of the studied pesticides for 20 days at a hydraulic loading rate (HLR) of 0.32 m day⁻¹. The results show that the sand filter was the best supporting material for removing phenoxyacid herbicides (77% on average), whereas peat soil and pine bark were best for removing non-ionic pesticides (72% on average). The attenuation of mecoprop and dichlorprop correlated negatively with the enantiomeric fraction. Therefore, this study shows that the use of waste-to-product materials in biofilter systems is a good solution for removing pollutants from agricultural run-off waters.

This study aims to investigate the single and binary biosorption of Cr(III) and Ni(II) by pine bark chemically treated with NaOH solution (MPB). The studies involved the effect of initial pH in the equilibrium, as well as kinetic uptake using synthetic solutions. Equilibrium tests were also conducted with an industrial effluent. The kinetic model of pseudo-second order described well the data of single and binary systems. The equilibrium data were better described by the Langmuir model for both metals. The maximum adsorption capacity (qₘₐₓ) to single system was 31.4 and 23.7 mg/g for Cr(III) and Ni(II), respectively. To analyse the competitive sorption between chromium and nickel ions, the modified Langmuir and Freundlich models were tested for two different concentration (mEq/L) ratios Cr(III)/Ni(II) of 1:1 and 2:1. The modified Langmuir model is also the best to fit the experimental data for both syntetic and industrial effluents. In the synthetic effluent, the qₘₐₓ value for Cr(III) in MPB was about 25 mg/g, while qₘₐₓ for Ni(II) decreased from 12.4 to 5.5 mg/g. The results showed that Ni(II) did not significantly interfere in Cr(III) adsorption capacity, whereas Cr(III) decreased the uptake of Ni(II). The industrial effluent contains several species, and thus, the sorption capacities for Cr(III) and Ni(II) were significantly affected.

The development of neuroprotective drugs through eco-friendly production routes is a major challenge for current pharmacology. The present study was carried out to synthesize gold nanoparticles (AuNPs) through biogenic route using ethanolic bark extract of Terminalia arjuna, a plant of high interest in Asian traditional medicine, and to evaluate its neuroprotective effects. The synthesized AuNPs were characterized by UV-Vis spectroscopy, FTIR spectroscopy, XRD, FESEM, EDX, HRTEM, DLS, and zeta potential analyses. UV-Vis spectroscopy showed a characteristics SPR absorption band at 536 nm specific for AuNPs. XRD, TEM, and FESEM analyses revealed the formation of face-centered cubic crystalline, spherical and triangular shaped AuNPs, with size ranging between 20 and 50 nm. DLS and ZP analysis illustrated that the average size of AuNPs was 30 nm, which was found to be stable at 45 mv. The neuroprotective potential of AuNPs was evaluated by assessing its antioxidant, cholinesterase inhibitory, and antiamyloidogenic activities. AuNPs showed dose-dependant inhibition of acetylcholinesterase and butyrylcholinesterase with IC₅₀ value of 4.25 ± 0.02 and 5.05 ± 0.02 μg/ml, respectively. In vitro antioxidant assays illustrated that AuNPs exhibited the highest reducing power and DPPH radical scavenging activity. In addition, AuNPs also efficiently suppressed the fibrillation of Aβ and destabilized the preformed mature fibrils. Results of toxicity studies in PBMC and adult zebra fish illustrated that AuNPs are non-toxic and biocompatible. Overall, our results highlighted the AuNPs promising potential in terms of antioxidant, anticholinesterase, antiamyloidogenic effects, and non-lethality allowing us to propose these nanomaterials as a suitable candidate for the development of drugs helpful in the treatment of neurodegenerative disorders like Alzheimer’s disease. Graphical abstract ᅟ

In vitro elicitation of an important compound conessine has been done in the bark-derived callus culture of Holarrhena antidysenterica (L.) Wall. employing different elicitors. For induction of callus, green bark explants excised from field-grown plants were cultured on MS medium augmented with different concentrations (0, 1, 2.5, 5, and 10 μM) of various growth regulators such as BA, IBA, NAA, and 2,4-D either alone or in combinations. The maximum amount of conessine (458.18 ± 0.89ᵈ μg/g dry wt.) was achieved in callus developed on MS medium supplemented with 5 μM BA and 5 μM 2,4-D through HPLC analysis. Elicitation in conessine content in the above callus was achieved employing a variety of organic (phenylalanine, tyrosine, chitosan, tryptophan, casein hydrolysate, proline, sucrose, and yeast extract) as well as inorganic elicitors (Pb(NO₃)₂, As₂O₃, CuSO₄, NaCl, and CdCl₂) in different concentrations. The optimum enhancement in conessine content (3518.58 ± 0.28ᵍ μg/g dry wt.) was seen at the highest concentration (200 mg/L) of phenylalanine. The enhancement was elicitor specific and dose dependent. The overall increment of the conessine content was seen in the order of phenylalanine > tryptophan > Pb(NO₃)₂ > sucrose > NaCl > As₂O₃ > casein hydrolysate > CdCl₂ > chitosan > proline > yeast extract > CuSO₄ > tyrosine. The isolation and purification of conessine was done using methanol as a solvent system through column chromatography (CC) and TLC. The isolated compound was characterized by FT-IR, ¹H-NMR, and HRMS which confirmed with the structure of conessine. The bioassays conducted with the isolated compound revealed a strong larvicidal activity against Anopheles stephensi Liston with LC₅₀ and LC₉₀ values being 1.93 and 5.67 ppm, respectively, without harming the nontarget organism, Mesocyclops thermocyclopoides Harada, after 48 h of treatment. This is our first report for the isolation and elicitation of conessine in the callus culture of H. antidysenterica.

Five plant extracts traditionally used in organic and biodynamic farming for pest control and antifungal (downy mildew) disease management were selected after a farmer survey and analyzed for their chemical composition in LC-PDA-MS-MS and using adapted analytical method from food chemistry for determination of class of component (e.g., protein, sugar, lipids…). Their antifungal activity against Penicillium expansum, Botrytis cinerea, Botrytis allii, brown rot causing agents (Monilinia laxa and Monilinia fructigena), and grape downy mildew (Plasmopara viticola) was examined in vitro. White willow (Salix alba) and absinthe (Artemisia absinthium) ethanolic extracts were found to be the most effective in particular against Plasmopara viticola, with a total inhibition of spores germination when applied at 1000 mg/L. These extracts also showed a relatively low toxicity during preliminary ecotoxicological assays on Daphnia pulex. Extract from the bark of white willow contained some flavonoids, especially flavanones (eriodyctiol and derivates) and flavanols (catechins and derivates), as major compounds, whereas absinthe extract was rich in O-methylated flavanols and hydroxycinnamic acids. Thujone content in this extract was also determined by external calibration in GC-MS analysis, and its value was 0.004% dry extract.