The river Timok with its watershed area is one of very imperilled running water on the territory of Serbia (Yugoslavia). By several years investigations of colonies bottoms (phytobenthos, microzoobenthos and macrozoobenthos) and plankton we have found the following: great diversity and specific phytobenthos and bottom fauna (frequency of certain taxa is considerably larger in relation to the other areas, and new taxa have been recorded). Stated specific structure of phytobenthos and bottom fauna have considerably reflected to right estimation of water quality of tested area.

There were estimated of water quality of the river Banja and its trubutary Pocibrava, Serbia (Yugoslavia), during the period 1991-1994 by seasonal investigations of benthos fauna composition as bioindicators. The results have shown higher diversity of the Pocibrava bottom fauna community (66 taxa) than in the river Banja (53 taxa) where it was more uniform. Both water flows were receiving some organic loading and by the use of macrobenthos indicator organisms it was concluded that they belong to beta-mesosaprobic water quality class.

During hydrobiological investigations of the river Dulenska (Serbia, Yugoslavia) in June 1996, algological samples were taken at this river. In the algae community are found 34 taxa from two divisio: Bacillariophyta (28) and Chlorophyta (6). While qualitative composition of the algae colony was relativelly uniformed quantitative one was changeable along the course of the Dulenska river. By saprobiological analysis, it was found that the quality of water was changing along the course of the river. At the upper and middle course of river the water belonging to the second class. At lower course of the river (below Rekovac) water quality was getfing worse and it belonging to the third class.

During one-year examination of the Vlasina river and its main tributaries, the Luznica and Gradska rivers, Serbia (Yugoslavia), in May 1996, the samples were taken for biological and chemical analysis of the watercourse in the spring aspect. Sampling was performed on the five localities of the Vlasina river, and two tribute localities near the mouth thus including all critical points where the changes of physico-chemical water parameters and qualitative/quantitative biocenoses compositions due to the increasing anthropogenic influence could occur. Data on phytoplankton, zooplankton, periphyton and benthos were analysed together with the physico-chemical water parameters in order to determine communities composition and structure and bioindicator species. Based on these results the status of aquatic environment i.e. water quality, was evaluated.