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The environmental contaminant tributyltin leads to abnormalities in different levels of the hypothalamus-pituitary-thyroid axis in female rats
2018
Andrade, Marcelle Novaes | Santos-Silva, Ana Paula | Rodrigues-Pereira, Paula | Paiva-Melo, Francisca Diana | de Lima Junior, Niedson Correa | Teixeira, Mariana Pires | Soares, Paula | Dias, Glaecir Roseni Munstock | Graceli, Jones Bernardes | de Carvalho, Denise Pires | Ferreira, Andrea Claudia Freitas | Miranda-Alves, Leandro
Tributyltin is a biocide used in nautical paints, aiming to reduce fouling of barnacles in ships. Despite the fact that many effects of TBT on marine species are known, studies in mammals have been limited, especially those evaluating its effect on the function of the hypothalamus-pituitary-thyroid (HPT) axis. The aim of this study was to investigate the effects of subchronic exposure to TBT on the HPT axis in female rats. Female Wistar rats received vehicle, TBT 200 ng kg−1 BW d−1 or 1000 ng kg−1 BW d−1 orally by gavage for 40 d. Hypothalamus, pituitary, thyroid, liver and blood samples were collected. TBT200 and TBT1000 thyroids showed vacuolated follicular cells, with follicular hypertrophy and hyperplasia. An increase in epithelial height and a decrease in the thyroid follicle and colloid area were observed in TBT1000 rats. Moreover, an increase in the epithelium/colloid area ratio was observed in both TBT groups. Lower TRH mRNA expression was observed in the hypothalami of TBT200 and TBT1000 rats. An increase in Dio1 mRNA levels was observed in the hypothalamus and thyroid in TBT1000 rats only. TSH serum levels were increased in TBT200 rats. In TBT1000 rats, there was a decrease in total T4 serum levels compared to control rats, whereas T3 serum levels did not show significant alterations. We conclude that TBT exposure can promote critical abnormalities in the HPT axis, including changes in TRH mRNA expression and serum TSH and T4 levels, in addition to affecting thyroid morphology. These findings demonstrate that TBT disrupts the HPT axis. Additionally, the changes found in thyroid hormones suggest that TBT may interfere with the peripheral metabolism of these hormones, an idea corroborated by the observed changes in Dio1 mRNA levels. Therefore, TBT exposition might interfere not only with the thyroid axis but also with thyroid hormone metabolism.
Show more [+] Less [-]Occurrence and overlooked sources of the biocide carbendazim in wastewater and surface water
2018
Merel, Sylvain | Benzing, Saskia | Gleiser, Carolin | Di Napoli-Davis, Gina | Zwiener, Christian
Carbendazim is a fungicide commonly used as active substance in plant protection products and biocidal products, for instance to protect facades of buildings against fungi. However, the subsequent occurrence of this fungicide and potential endocrine disruptor in the aqueous environment is a major concern. In this study, high resolution mass spectrometry shows that carbendazim can be detected with an increasing abundance from the source to the mouth of the River Rhine. Unexpectedly, the abundance of carbendazim correlates poorly with that of other fungicides used as active ingredients in plant protection products (r² of 0.32 for cyproconazole and r² of 0.57 for propiconazole) but it correlates linearly with that of pharmaceuticals (r² of 0.86 for carbamazepine and r² of 0.89 for lamotrigine). These results suggest that the occurrence of carbendazim in surface water comes mainly from the discharge of treated domestic wastewater. This hypothesis is further confirmed by the detection of carbendazim in wastewater effluents (n = 22). In fact, bench-scale leaching tests of textiles and papers revealed that these materials commonly found in households could be a source of carbendazim in domestic wastewater. Moreover, additional river samples collected nearby two paper industries indicate that the discharge of their treated process effluents is also a source of carbendazim in the environment. While characterizing paper and textile as overlooked sources of carbendazim, this study also shows the biocide as a possible ubiquitous wastewater contaminant that would require further systematic and worldwide monitoring due to its toxicological properties.
Show more [+] Less [-]Emergent synergistic lysosomal toxicity of chemical mixtures in molluscan blood cells (hemocytes)
2018
Moore, M.N. | Wedderburn, R.J. | Clarke, K.R. | McFadzen, I.R.B. | Lowe, D.M. | Readman, J.W.
The problem of effective assessment of risk posed by complex mixtures of toxic chemicals in the environment is a major challenge for government regulators and industry. The biological effect of the individual contaminants, where these are known, can be measured; but the problem lies in relating toxicity to the multiple constituents of contaminant cocktails. The objective of this study was to test the hypothesis that diverse contaminant mixtures may cause a greater toxicity than the sum of their individual parts, due to synergistic interactions between contaminants with different intracellular targets. Lysosomal membrane stability in hemocytes from marine mussels was used for in vitro toxicity tests; and was coupled with analysis using the isobole method and a linear additive statistical model. The findings from both methods have shown significant emergent synergistic interactions between environmentally relevant chemicals (i.e., polycyclic aromatic hydrocarbons, pesticides, biocides and a surfactant) when exposed to isolated hemocytes as a mixture of 3 & 7 constituents. The results support the complexity-based hypothesis that emergent toxicity occurs with increasing contaminant diversity, and raises questions about the validity of estimating toxicity of contaminant mixtures based on the additive toxicity of single components. Further experimentation is required to investigate the potential for interactive effects in mixtures with more constituents (e.g., 50–100) at more environmentally realistic concentrations in order to test other regions of the model, namely, very low concentrations and high diversity. Estimated toxicant diversity coupled with tests for lysosomal damage may provide a potential tool for determining the toxicity of estuarine sediments, dredge spoil or contaminated soil.
Show more [+] Less [-]Evaluation of polar organic micropollutants as indicators for wastewater-related coastal water quality impairment
2016
Nödler, Karsten | Tsakiri, Maria | Aloupi, Maria | Gatidou, Georgia | Stasinakis, Athanasios S. | Licha, Tobias
Results from coastal water pollution monitoring (Lesvos Island, Greece) are presented. In total, 53 samples were analyzed for 58 polar organic micropollutants such as selected herbicides, biocides, corrosion inhibitors, stimulants, artificial sweeteners, and pharmaceuticals. Main focus is the application of a proposed wastewater indicator quartet (acesulfame, caffeine, valsartan, and valsartan acid) to detect point sources and contamination hot-spots with untreated and treated wastewater. The derived conclusions are compared with the state of knowledge regarding local land use and infrastructure. The artificial sweetener acesulfame and the stimulant caffeine were used as indicators for treated and untreated wastewater, respectively. In case of a contamination with untreated wastewater the concentration ratio of the antihypertensive valsartan and its transformation product valsartan acid was used to further refine the estimation of the residence time of the contamination. The median/maximum concentrations of acesulfame and caffeine were 5.3/178 ng L⁻¹ and 6.1/522 ng L⁻¹, respectively. Their detection frequency was 100%. Highest concentrations were detected within the urban area of the capital of the island (Mytilene). The indicator quartet in the gulfs of Gera and Kalloni (two semi-enclosed embayments on the island) demonstrated different concentration patterns. A comparatively higher proportion of untreated wastewater was detected in the gulf of Gera, which is in agreement with data on the wastewater infrastructure. The indicator quality of the micropollutants to detect wastewater was compared with electrical conductivity (EC) data. Due to their anthropogenic nature and low detection limits, the micropollutants are superior to EC regarding both sensitivity and selectivity. The concentrations of atrazine, diuron, and isoproturon did not exceed the annual average of their environmental quality standards (EQS) defined by the European Commission. At two sampling locations irgarol 1051 exceeded its annual average EQS value but not the maximum allowable concentration of 16 ng L⁻¹.
Show more [+] Less [-]Revealing ecological risks of priority endocrine disrupting chemicals in four marine protected areas in Hong Kong through an integrative approach
2016
Xu, Elvis Genbo | Ho, Philip Wing-Lok | Tse, Zero | Ho, Shu-Leong | Leung, Kenneth Mei Yee
Marine Protected Areas (MPAs) in Hong Kong are situated in close proximity to urbanized areas, and inevitably influenced by wastewater discharges and antifouling biocides leached from vessels. Hence, marine organisms inhabiting these MPAs are probably at risk. Here an integrative approach was employed to comprehensively assess ecological risks of eight priority endocrine disrupting chemicals (EDCs) in four MPAs of Hong Kong. We quantified their concentrations in environmental and biota samples collected in different seasons during 2013–2014, while mussels (Septifer virgatus) and semi-permeable membrane devices were deployed to determine the extent of accumulation of the EDCs. Extracts from the environmental samples were subjected to the yeast estrogen screen and a novel human cell-based catechol-O-methyltransferase ELISA to evaluate their estrogenic activities. The results indicated ecological risks of EDCs in the Cape d'Aguilar Marine Reserve. This integrated approach can effectively evaluate ecological risks of EDCs through linking their concentrations to biological effects.
Show more [+] Less [-]Biocides in the Yangtze River of China: Spatiotemporal distribution, mass load and risk assessment
2015
Liu, Wang-Rong | Zhao, Jian-Liang | Liu, You-Sheng | Chen, Zhi-Feng | Yang, Yuan-Yuan | Zhang, Qian-Qian | Ying, Guang-Guo
Nineteen biocides were investigated in the Yangtze River to understand their spatiotemporal distribution, mass loads and ecological risks. Fourteen biocides were detected, with the highest concentrations up to 166 ng/L for DEET in surface water, and 54.3 ng/g dry weight (dw) for triclocarban in sediment. The dominant biocides were DEET and methylparaben, with their detection frequencies of 100% in both phases. An estimate of 152 t/y of 14 biocides was carried by the Yangtze River to the East China Sea. The distribution of biocides in the aquatic environments was significantly correlated to Gross Domestic Product (GDP), total phosphorus (TP) and total nitrogen (TN), suggesting dominant input sources from domestic wastewater of the cities along the river. Risk assessment showed high ecological risks posed by carbendazim in both phases and by triclosan in sediment. Therefore, proper measures should be taken to reduce the input of biocides into the river systems.
Show more [+] Less [-]Transformation and release of micronized Cu used as a wood preservative in treated wood in wetland soil
2021
Johnson, M.G. | Luxton, T.P. | Rygiewicz, P.T. | Reichman, J.R. | Bollman, M.A. | King, G.A. | Storm, M.J. | Nash, M.S. | Andersen, C.P.
Micronized Cu (μ-Cu) is used as a wood preservative, replacing toxic chromated copper arsenate (CCA). Micronized Cu is malachite [Cu₂CO₃(OH)₂] that has been milled to micron/submicron particles, with many particle diameters less than 100 nm, mixed with biocides and then used to treat wood. In addition to concerns about the fate of the Cu from μ-Cu, there is interest in the fate of the nano-Cu (n-Cu) constituents. We examined movement of Cu from μ-Cu-treated wood after placing treated-wood stakes into model wetland ecosystems. Release of Cu into surface and subsurface water was monitored. Surface water Cu reached maximum levels 3 days after stake installation and remained elevated if the systems remained inundated. Subsurface water Cu levels were 10% of surface water levels at day 3 and increased gradually thereafter. Sequential filtering indicated that a large portion of the Cu in solution was associating with soluble organics, but there was no evidence for n-Cu in solution. After 4 months, Cu in thin-sections of treated wood and adjacent soil were characterized with micro X-ray absorption fine structure spectroscopy (μ-XAFS). Localization and speciation of Cu in the wood and adjacent soil using μ-XAFS clearly indicated that Cu concentrations decreased over time in the treated wood and increased in the adjacent soil. However, n-Cu from the treated wood was not found in the adjacent soil or plant roots. The results of this study indicate that Cu in the μ-Cu-treated wood dissolves and migrates into adjacent soil and waters primarily in ionic form (i.e., Cu²⁺) and not as nano-sized Cu particles. A reduced form of Cu (Cu₂S) was identified in deep soil proximal to the treated wood, indicating strong reducing conditions. The formation of the insoluble Cu₂S effectively removes some portion of dissolved Cu from solution, reducing movement of Cu²⁺ to the water column and diminishing exposure.
Show more [+] Less [-]Investigating the effects of municipal and hospital wastewaters on horizontal gene transfer
2021
Hutinel, Marion | Fick, Jerker | Larsson, D.G Joakim | Flach, Carl-Fredrik
Horizontal gene transfer (HGT) plays an important role in the dissemination of antibiotic resistance genes. In sewer systems, human-associated and environmental bacteria are mixed together and exposed to many substances known to increase HGT, including various antibacterial compounds. In wastewaters, those substances are most often detected below concentrations known to induce HGT individually. Still, it is possible that such wastewaters induce HGT, for example via mixture effects. Here, a panel of antibiotics, biocides and other pharmaceuticals was measured in filter-sterilized municipal and hospital wastewater samples from Gothenburg, Sweden. The effects on HGT of the chemical mixtures in these samples were investigated by exposing a complex bacterial donor community together with a GFP-tagged E. coli recipient strain. Recipients that captured sulfonamide resistance-conferring mobile genetic elements (MGEs) from the bacterial community were enumerated and characterized by replicon typing, antibiotic susceptibility testing and long read sequencing. While exposure to municipal wastewater did not result in any detectable change in HGT rates, exposure to hospital wastewater was associated with an increase in the proportion of recipients that acquired sulfonamide resistance but also a drastic decrease in the total number of recipients. Although, concentrations were generally higher in hospital than municipal wastewater, none of the measured substances could individually explain the observed effects of hospital wastewater. The great majority of the MGEs captured were IncN plasmids, and resistance to several antibiotics was co-transferred in most cases. Taken together, the data show no evidence that chemicals present in the studied municipal wastewater induce HGT. Still, the increased relative abundance of transconjugants after exposure to hospital wastewater could have implications for the risks of both emergence and transmission of resistant bacteria.
Show more [+] Less [-]A flow-through aqueous standard generation system for thin film microextraction investigations of UV filters and biocides partitioning to different environmental compartments
2017
Ahmadi, Fardin | Sparham, Chris | Pawliszyn, Janusz
In this paper problems associated with preparation of aqueous standard of highly hydrophobic compounds such as partial precipitation, being lost on the surfaces, low solubility in water and limited sample volume for accurate determination of their distribution coefficients are addressed. The following work presents two approaches that utilize blade thin film microextraction (TFME) to investigate partitioning of UV filters and biocides to humic acid (dissolved organic carbon) and sediment. A steady-state concentration of target analytes in water was generated using a flow-through aqueous standard generation (ASG) system. Dialysis membranes, a polytetrafluoroethylene permeation tube, and a frit porous (0.5 μm) coated by epoxy glue were basic elements used for preparation of the ASG system. In the currently presented study, negligible depletion TFME using hydrophilic-lipophilic balance (HLB) and octadecyl silica-based (C18) sorbents was employed towards the attainment of free concentration values of target analytes in the studied matrices. Thin film geometry provided a large volume of extraction phase, which improved the sensitivity of the method towards highly matrix-bound analytes. Extractions were performed in the equilibrium regime so as to prevent matrix effects and with aims to reach maximum method sensitivity for all analytes under study. Partitioning of analytes on dissolved organic carbon (DOC) was investigated in ASG to facilitate large sample volume conditions. Binding percentages and DOC distribution coefficients (Log KDOC) ranged from 20 to 98% and 3.71–6.72, respectively. Furthermore, sediment-water partition coefficients (Kd), organic-carbon normalized partition coefficients (Log KOC), and DOC distribution coefficients (Log KDOC) were investigated in slurry sediment, and ranged from 33 to 2860, 3.31–5.24 and 4.52–5.75 Lkg-1, respectively. The obtained results demonstrated that investigations utilizing ASG and TFME can yield reliable binding information for compounds with high log KOW values. This information is useful for study of fate, transport, and ecotoxicological effects of UV filters and biocides in aquatic environment.
Show more [+] Less [-]Hazard assessment of chemical constituents in biocide formulations used in offshore oil and gas operations
2022
Bejarano, Adriana C. | Hughes, Sarah A. | Saunders, David
Biocides used in offshore oil and gas operations could be present in water discharges, and thus identifying such chemicals and their hazard could help address concerns regarding non-target organisms. Aquatic toxicity data, queried from different sources and augmented with predictive models, were used to develop species sensitivity distributions and their corresponding 5th percentile hazard concentrations (HC5s). Curated data, including over 1000 empirical records for 137 species, indicated no evidence of bias when comparing sensitivity between marine and freshwater species, even when predicted data were used. HC5s facilitated estimation of an acute-to-chronic ratio (ACR = 10), appropriate for most chemicals and useful in filling data gaps. Comparison of chronic-HC5s with the default approach for deriving predicted no effect concentrations showed that the latter systematically overstates aquatic hazard. The present approach shows promise of using acute-to-chronic HC5 ratios for defining assessment factors for different chemical classes, instead of the use of generic assessment factors.
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